################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbrPI BbvCI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaI BseAI BsiEI BsiWI BsmI Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I CelII CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MfeI MluI MroI MunI NaeI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfuI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccII 372 CGCG CG^CG AcyI 3 GGCGCC G[GA]^CG[CT]C AgeI 600 ACCGGT A^CCGGT AlwNI 1556 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 451 CCTGAGG CC^T[ACGT]AGG Asp700I 165 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanII 1176 GGGCTC G[GA]GC[CT]^C BbeI 3 GGCGCC GGCGC^C BcgI 374 CGAAGGTGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1355 AGATCT A^GATCT BpmI 80 CTGGAG CTGGAG(16/14) BsaBI 636 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 3 GGCGCC G[GA]^CG[CT]C BsgI 701 GTGCAG GTGCAG(16/14) BsmBI 796 GAGACG CGTCTC(1/5) BspDI 250 ATCGAT AT^CGAT BsrDI 1457 GCAATG GCAATG(2/0) BsrFI 600 ACCGGT [GA]^CCGG[CT] BssSI 693 CACGAG CACGAG(-5/-1) BstEII 243 GGTCACC G^GT[ACGT]ACC BstPI 243 GGTCACC G^GT[ACGT]ACC BstUI 372 CGCG CG^CG Bsu36I 451 CCTGAGG CC^T[ACGT]AGG Cfr10I 600 ACCGGT [GA]^CCGG[CT] ClaI 250 ATCGAT AT^CGAT DraII 338 AGGCCCC [GA]G^G[ACGT]CC[CT] DrdI 235 GACCTGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 385 CCATGG C^C[GA][CT]GG Eco81I 451 CCTGAGG CC^T[ACGT]AGG EcoO109I 338 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoO65I 243 GGTCACC G^GT[ACGT]ACC EcoRV 121 GATATC GAT^ATC Hin1I 3 GGCGCC G[GA]^CG[CT]C KasI 3 GGCGCC G^GCGCC MamI 636 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 632 TTAA T^TAA MvnI 372 CGCG CG^CG NarI 3 GGCGCC GG^CGCC NcoI 385 CCATGG C^CATGG NheI 907 GCTAGC G^CTAGC PflMI 765 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 600 ACCGGT A^CCGGT PstI 1553 CTGCAG CTGCA^G ScaI 101 AGTACT AGT^ACT SfoI 3 GGCGCC GGC^GCC SgrAI 599 CACCGGTG C[GA]^CCGG[CT]G SmaI 1331 CCCGGG CCC^GGG Tru9I 632 TTAA T^TAA Van91I 765 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 297 TCTAGA T^CTAGA XcmI 27 CCAGCGGGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1331 CCCGGG C^CCGGG XmaI 1331 CCCGGG C^CCGGG XmnI 165 GAACAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AvaII 819 GGACC G^G[AT]CC 987 GGTCC BalI 39 TGGCCA TGG^CCA 1467 TGGCCA BbsI 540 GAAGAC GAAGAC(2/6) 619 GTCTTC BpuAI 540 GAAGAC GAAGAC(2/6) 619 GTCTTC BstXI 188 CCACTTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1533 CCAGGAGGATGG EaeI 39 TGGCCA [CT]^GGCC[GA] 1467 TGGCCA FauI 30 GCGGG CCCGC(4/6) 816 GCGGG MaeII 799 ACGT A^CGT 893 ACGT MluNI 39 TGGCCA TGG^CCA 1467 TGGCCA MscI 39 TGGCCA TGG^CCA 1467 TGGCCA MslI 208 CACAAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 597 CACACCGGTG PvuII 305 CAGCTG CAG^CTG 704 CAGCTG SfcI 788 CTGTAG C^T[GA][CT]AG 1553 CTGCAG Tsp509I 52 AATT ^AATT 870 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaI BanI BbvI BcnI BfaI BmyI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XhoII