(Circular) (Six-base) MAPSORT of: pFN21AE3585.seq Check: 2596 from: 1 to: 6298 pFN21AE3585 6298 bp With 182 enzymes: SgfI * March 28, 2012 12:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4108 278 Size: 53 83 186 936 2572 2468 Fragments arranged by size: 2572 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3641 1937 Size: 1704 4594 Acc65I G'GTAC_C Cuts at: 3619 3619 Size: 6298 AclI AA'CG_TT Cuts at: 1563 4425 4798 1563 Size: 2862 373 3063 Fragments arranged by size: 3063 2862 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4361 5745 6013 1632 Size: 2729 1384 268 1917 Fragments arranged by size: 2729 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6101 AflIII A'CryG_T Cuts at: 1203 5197 6174 1203 Size: 3994 977 1327 Fragments arranged by size: 3994 1327 977 AgeI A'CCGG_T Cuts at: 2605 5129 2605 Size: 2524 3774 AhdI GACnn_n'nnGTC Cuts at: 5027 5027 Size: 6298 AleI CACnn'nnGTG Cuts at: 1166 2606 1166 Size: 1440 4858 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6298 AlwNI CAG_nnn'CTG Cuts at: 3566 4041 5613 3566 Size: 475 1572 4251 Fragments arranged by size: 4251 1572 475 ApaLI G'TGCA_C Cuts at: 4355 5511 4355 Size: 1156 5142 ApoI r'AATT_y Cuts at: 1493 3607 3833 3985 6094 1493 Size: 2114 226 152 2109 1697 Fragments arranged by size: 2114 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4852 160 Size: 872 3820 1606 Fragments arranged by size: 3820 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 AvaI C'yCGr_G Cuts at: 1870 1943 2595 2880 3244 3336 3623 1870 Size: 73 652 285 364 92 287 4545 Fragments arranged by size: 4545 652 364 287 285 92 73 BamHI G'GATC_C Cuts at: 3628 3995 3628 Size: 367 5931 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2386 3035 3619 5074 Size: 325 217 627 221 378 649 584 1455 Cuts at: 5074 618 Size: 1842 Fragments arranged by size: 1842 1455 649 627 584 378 325 221 217 BanII G_rGCy'C Cuts at: 1391 3185 3617 1391 Size: 1794 432 4072 Fragments arranged by size: 4072 1794 432 BbeI G_GCGC'C Cuts at: 2012 2012 Size: 6298 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2552 2617 928 Size: 971 653 65 4609 Fragments arranged by size: 4609 971 653 65 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2368 2402 4489 4523 1903 Size: 34 431 34 2087 34 3678 Fragments arranged by size: 3678 2087 431 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4190 5400 4190 Size: 1210 5088 BclI T'GATC_A Cuts at: 1548 1548 Size: 6298 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4909 136 Size: 107 122 71 631 605 3237 1525 Fragments arranged by size: 3237 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3360 6293 3360 Size: 2933 3365 BlpI GC'TnA_GC Cuts at: 3715 3715 Size: 6298 Bme1580I G_kGCm'C Cuts at: 1790 4359 5515 1790 Size: 2569 1156 2573 Fragments arranged by size: 2573 2569 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6298 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3510 4029 4987 Size: 416 53 83 399 24 2093 519 958 Cuts at: 4987 442 Size: 1753 Fragments arranged by size: 2093 1753 958 519 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2916 1056 Size: 1860 4438 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2431 2431 Size: 6298 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2106 4958 1239 Size: 867 2852 2579 Fragments arranged by size: 2852 2579 867 Bpu10I CC'TnA_GC Cuts at: 2661 3093 2661 Size: 432 5866 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4423 5288 5586 4423 Size: 865 298 5135 Fragments arranged by size: 5135 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4961 882 Size: 617 3462 2219 Fragments arranged by size: 3462 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4089 493 Size: 711 2885 2702 Fragments arranged by size: 2885 2702 711 BsaBI GATnn'nnATC Cuts at: 2645 6292 2645 Size: 3647 2651 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 4105 4487 5122 Size: 53 83 186 936 476 2096 382 635 Cuts at: 5122 275 Size: 1451 Fragments arranged by size: 2096 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2064 2605 3130 4731 5129 5403 5550 5961 2064 Size: 541 525 1601 398 274 147 411 2401 Fragments arranged by size: 2401 1601 541 525 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2208 2238 2711 2741 1178 Size: 30 165 30 805 30 473 30 4735 Fragments arranged by size: 4735 805 473 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2348 2846 2849 3149 3164 3206 3371 1186 Size: 1162 498 3 300 15 42 165 4113 Fragments arranged by size: 4113 1162 498 300 165 42 15 3 BseYI C'CCAG_C Cuts at: 2031 2367 2683 3020 3111 3176 5501 5997 2031 Size: 336 316 337 91 65 2325 496 2332 Fragments arranged by size: 2332 2325 496 337 336 316 91 65 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2727 1163 Size: 573 991 4734 Fragments arranged by size: 4734 991 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3748 4509 5537 1540 Size: 275 186 1747 761 1028 2301 Fragments arranged by size: 2301 1747 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2662 3243 3531 4359 4444 5515 2662 Size: 581 288 828 85 1071 3445 Fragments arranged by size: 3445 1071 828 581 288 85 BsmI GAATG_Cn' Cuts at: 3809 3902 3809 Size: 93 6205 BsmBI CGTCTCn'nnnn_ Cuts at: 2795 2795 Size: 6298 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2662 3185 3243 3531 3617 4359 4444 Size: 399 872 523 58 288 86 742 85 Cuts at: 4444 5515 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 872 742 523 399 288 86 85 58 BspEI T'CCGG_A Cuts at: 5961 5961 Size: 6298 BspHI T'CATG_A Cuts at: 4187 5917 4187 Size: 1730 4568 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3653 844 Size: 2809 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4185 1394 Size: 429 2362 3507 Fragments arranged by size: 3507 2362 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3469 4793 4967 65 Size: 1183 2221 1324 174 1396 Fragments arranged by size: 2221 1396 1324 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2605 4942 5129 1700 Size: 110 795 2337 187 2869 Fragments arranged by size: 2869 2337 795 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6298 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6298 BssSI C'ACGA_G Cuts at: 2698 4358 5370 2698 Size: 1660 1012 3626 Fragments arranged by size: 3626 1660 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6298 BstBI TT'CG_AA Cuts at: 5102 5102 Size: 6298 BstEII G'GTnAC_C Cuts at: 1184 2248 1184 Size: 1064 5234 BstXI CCAn_nnnn'nTGG Cuts at: 2200 3545 2200 Size: 1345 4953 BstYI r'GATC_y Cuts at: 1972 2305 2363 3360 3628 3995 4380 4397 5849 Size: 333 58 997 268 367 385 17 1452 Cuts at: 5849 5935 5947 6293 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 997 385 367 346 333 268 86 58 17 12 Bsu36I CC'TnA_GG Cuts at: 2457 2457 Size: 6298 BtgI C'CryG_G Cuts at: 513 1065 2004 2390 513 Size: 552 939 386 4421 Fragments arranged by size: 4421 939 552 386 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5559 Fragments arranged by size: 5559 683 56 BtsI GCAGTG_nn' Cuts at: 3220 3235 3310 3376 3442 3556 3814 4608 4628 Size: 15 75 66 66 114 258 794 20 Cuts at: 4628 3220 Size: 4890 Fragments arranged by size: 4890 794 258 114 75 66 66 20 15 ClaI AT'CG_AT Cuts at: 1554 2256 1554 Size: 702 5596 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3451 3486 529 Size: 35 2887 35 3341 Fragments arranged by size: 3341 2887 35 35 DraI TTT'AAA Cuts at: 3602 3602 Size: 6298 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2246 5305 809 Size: 507 930 3059 1802 Fragments arranged by size: 3059 1802 930 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2044 3472 3745 Size: 54 1425 50 275 18 214 1428 273 Cuts at: 3745 4634 6263 8 Size: 889 1629 43 Fragments arranged by size: 1629 1428 1425 889 275 273 214 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3745 1537 Size: 275 1933 4090 Fragments arranged by size: 4090 1933 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2325 2793 3135 3141 3159 4228 1353 Size: 972 468 342 6 18 1069 3423 Fragments arranged by size: 3423 1069 972 468 342 18 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2984 4885 5259 5405 387 Size: 1064 138 1395 1901 374 146 1280 Fragments arranged by size: 1901 1395 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2106 4361 4958 5745 6013 1239 Size: 393 474 2255 597 787 268 1524 Fragments arranged by size: 2255 1524 787 597 474 393 268 EcoO109I rG'GnC_Cy Cuts at: 2344 2344 Size: 6298 EcoRI G'AATT_C Cuts at: 3607 3985 3607 Size: 378 5920 EcoRV GAT'ATC Cuts at: 2128 2128 Size: 6298 FspI TGC'GCA Cuts at: 4804 4804 Size: 6298 HaeII r_GCGC'y Cuts at: 1678 2012 3010 3504 5445 1678 Size: 334 998 494 1941 2531 Fragments arranged by size: 2531 1941 998 494 334 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2711 2713 2743 2745 3227 Size: 32 135 32 1139 2 30 2 482 Cuts at: 3227 3259 3383 3415 4939 4971 5013 5045 1373 Size: 32 124 32 1524 32 42 32 2626 Fragments arranged by size: 2626 1524 1139 482 135 124 42 32 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 3642 3888 669 Size: 988 281 1704 246 3079 Fragments arranged by size: 3079 1704 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6298 HpaI GTT'AAC Cuts at: 3888 3888 Size: 6298 KasI G'GCGC_C Cuts at: 2008 2008 Size: 6298 KpnI G_GTAC'C Cuts at: 3623 3623 Size: 6298 MfeI C'AATT_G Cuts at: 3897 3897 Size: 6298 MluI A'CGCG_T Cuts at: 6174 6174 Size: 6298 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2697 2961 5412 5596 1305 Size: 1392 264 2451 184 2007 Fragments arranged by size: 2451 2007 1392 264 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2046 3474 10 Size: 54 1425 343 214 1428 2834 Fragments arranged by size: 2834 1428 1425 343 214 54 MslI CAynn'nnrTG Cuts at: 518 1166 2217 2606 4256 4615 4774 518 Size: 648 1051 389 1650 359 159 2042 Fragments arranged by size: 2042 1650 1051 648 389 359 159 MspA1I CmG'CkG Cuts at: 1606 2035 2312 2711 3456 3478 3714 4391 5539 Size: 429 277 399 745 22 236 677 1148 Cuts at: 5539 5784 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 745 677 429 399 277 245 236 22 NarI GG'CG_CC Cuts at: 2009 2009 Size: 6298 NcoI C'CATG_G Cuts at: 513 1065 2004 2390 513 Size: 552 939 386 4421 Fragments arranged by size: 4421 939 552 386 NdeI CA'TA_TG Cuts at: 387 387 Size: 6298 NheI G'CTAG_C Cuts at: 1052 2912 1052 Size: 1860 4438 NotI GC'GGCC_GC Cuts at: 3745 3745 Size: 6298 NspI r_CATG'y Cuts at: 1132 1229 3656 5201 1132 Size: 97 2427 1545 2229 Fragments arranged by size: 2427 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6298 PciI A'CATG_T Cuts at: 5197 5197 Size: 6298 PflMI CCAn_nnn'nTGG Cuts at: 1108 2776 1108 Size: 1668 4630 PmeI GTTT'AAAC Cuts at: 3602 3602 Size: 6298 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4367 4399 5880 5912 4367 Size: 32 1481 32 4753 Fragments arranged by size: 4753 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6298 PsiI TTA'TAA Cuts at: 3868 3868 Size: 6298 PstI C_TGCA'G Cuts at: 830 3562 3650 830 Size: 2732 88 3478 Fragments arranged by size: 3478 2732 88 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6298 PvuII CAG'CTG Cuts at: 2312 2711 2312 Size: 399 5899 SalI G'TCGA_C Cuts at: 1936 3640 1936 Size: 1704 4594 SbfI CC_TGCA'GG Cuts at: 3650 3650 Size: 6298 ScaI AGT'ACT Cuts at: 2108 4546 6258 2108 Size: 2438 1712 2148 Fragments arranged by size: 2438 2148 1712 SfcI C'TryA_G Cuts at: 826 1045 2793 3558 3646 4781 5462 5653 826 Size: 219 1748 765 88 1135 681 191 1471 Fragments arranged by size: 1748 1471 1135 765 681 219 191 88 SfoI GGC'GCC Cuts at: 2010 2010 Size: 6298 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 SgrAI Cr'CCGG_yG Cuts at: 1700 2605 1700 Size: 905 5393 SmaI CCC'GGG Cuts at: 1872 3338 3625 1872 Size: 1466 287 4545 Fragments arranged by size: 4545 1466 287 SmlI C'TyrA_G Cuts at: 820 1017 1943 4402 5303 5565 820 Size: 197 926 2459 901 262 1553 Fragments arranged by size: 2459 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4089 493 Size: 3596 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6298 SphI G_CATG'C Cuts at: 1132 3656 1132 Size: 2524 3774 SrfI GCCC'GGGC Cuts at: 3338 3338 Size: 6298 SspI AAT'ATT Cuts at: 5 52 4222 5 Size: 47 4170 2081 Fragments arranged by size: 4170 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6298 StyI C'CwwG_G Cuts at: 513 1065 2004 2199 2390 2691 3015 3427 3737 Size: 552 939 195 191 301 324 412 310 Cuts at: 3737 513 Size: 3074 Fragments arranged by size: 3074 939 552 412 324 310 301 195 191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4325 4342 4495 4680 5125 325 Size: 1255 2745 17 153 185 445 1498 Fragments arranged by size: 2745 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2106 2184 2409 Size: 274 80 33 51 1443 129 78 225 Cuts at: 2409 2886 4544 6256 96 Size: 477 1658 1712 138 Fragments arranged by size: 1712 1658 1443 477 274 225 138 129 80 78 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6258 6290 6258 Size: 32 6266 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6298 XbaI T'CTAG_A Cuts at: 2302 3634 2302 Size: 1332 4966 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2039 2039 Size: 6298 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6298 XmaI C'CCGG_G Cuts at: 1870 3336 3623 1870 Size: 1466 287 4545 Fragments arranged by size: 4545 1466 287 XmnI GAAnn'nnTTC Cuts at: 2174 4427 2174 Size: 2253 4045 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4106 276 Size: 53 83 186 936 2572 2468 Fragments arranged by size: 2572 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SwaI