(Linear) (Six-base) MAPSORT of: KIEE3585.seq Check: 4857 from: 1 to: 1593 KIEE3585.orf With 182 enzymes: SgfI * March 22, 2012 16:36 .. AgeI A'CCGG_T Cuts at: 0 600 1593 Size: 600 993 AleI CACnn'nnGTG Cuts at: 0 601 1593 Size: 601 992 AlwNI CAG_nnn'CTG Cuts at: 0 1561 1593 Size: 1561 32 AvaI C'yCGr_G Cuts at: 0 590 875 1239 1331 1593 Size: 590 285 364 92 262 Fragments arranged by size: 590 364 285 262 92 BanI G'GyrC_C Cuts at: 0 3 381 1030 1593 Size: 3 378 649 563 Fragments arranged by size: 649 563 378 3 BanII G_rGCy'C Cuts at: 0 1180 1593 Size: 1180 413 BbeI G_GCGC'C Cuts at: 0 7 1593 Size: 7 1586 BbsI GAAGACnn'nnnn_ Cuts at: 0 547 612 1593 Size: 547 65 981 Fragments arranged by size: 981 547 65 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 363 397 1593 Size: 363 34 1196 Fragments arranged by size: 1196 363 34 BglII A'GATC_T Cuts at: 0 1355 1593 Size: 1355 238 BmrI ACTGGGnnnn_n' Cuts at: 0 1505 1593 Size: 1505 88 BmtI G_CTAG'C Cuts at: 0 911 1593 Size: 911 682 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 426 1593 Size: 426 1167 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 101 1593 Size: 101 1492 Bpu10I CC'TnA_GC Cuts at: 0 656 1088 1593 Size: 656 432 505 Fragments arranged by size: 656 505 432 BsaBI GATnn'nnATC Cuts at: 0 640 1593 Size: 640 953 BsaHI Gr'CG_yC Cuts at: 0 4 1593 Size: 4 1589 BsaWI w'CCGG_w Cuts at: 0 59 600 1125 1593 Size: 59 541 525 468 Fragments arranged by size: 541 525 468 59 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 203 233 706 736 1593 Size: 203 30 473 30 857 Fragments arranged by size: 857 473 203 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 343 841 844 1144 1159 1201 1366 1593 Size: 343 498 3 300 15 42 165 227 Fragments arranged by size: 498 343 300 227 165 42 15 3 BseYI C'CCAG_C Cuts at: 0 26 362 678 1015 1106 1171 1593 Size: 26 336 316 337 91 65 422 Fragments arranged by size: 422 337 336 316 91 65 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 722 1593 Size: 722 871 BsiHKAI G_wGCw'C Cuts at: 0 657 1238 1526 1593 Size: 657 581 288 67 Fragments arranged by size: 657 581 288 67 BsmBI CGTCTCn'nnnn_ Cuts at: 0 790 1593 Size: 790 803 Bsp1286I G_dGCh'C Cuts at: 0 657 1180 1238 1526 1593 Size: 657 523 58 288 67 Fragments arranged by size: 657 523 288 67 58 BsrDI GCAATG_nn' Cuts at: 0 1464 1593 Size: 1464 129 BsrFI r'CCGG_y Cuts at: 0 600 1593 Size: 600 993 BssSI C'ACGA_G Cuts at: 0 693 1593 Size: 693 900 BstEII G'GTnAC_C Cuts at: 0 243 1593 Size: 243 1350 BstXI CCAn_nnnn'nTGG Cuts at: 0 195 1540 1593 Size: 195 1345 53 Fragments arranged by size: 1345 195 53 BstYI r'GATC_y Cuts at: 0 300 358 1355 1593 Size: 300 58 997 238 Fragments arranged by size: 997 300 238 58 Bsu36I CC'TnA_GG Cuts at: 0 452 1593 Size: 452 1141 BtgI C'CryG_G Cuts at: 0 385 1593 Size: 385 1208 BtsI GCAGTG_nn' Cuts at: 0 1215 1230 1305 1371 1437 1551 1593 Size: 1215 15 75 66 66 114 42 Fragments arranged by size: 1215 114 75 66 66 42 15 ClaI AT'CG_AT Cuts at: 0 251 1593 Size: 251 1342 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1446 1481 1593 Size: 1446 35 112 Fragments arranged by size: 1446 112 35 DrdI GACnn_nn'nnGTC Cuts at: 0 241 1593 Size: 241 1352 EaeI y'GGCC_r Cuts at: 0 39 1467 1593 Size: 39 1428 126 Fragments arranged by size: 1428 126 39 EarI CTCTTCn'nnn_ Cuts at: 0 320 788 1130 1136 1154 1593 Size: 320 468 342 6 18 439 Fragments arranged by size: 468 439 342 320 18 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 979 1593 Size: 979 614 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 101 1593 Size: 101 1492 EcoO109I rG'GnC_Cy Cuts at: 0 339 1593 Size: 339 1254 EcoRV GAT'ATC Cuts at: 0 123 1593 Size: 123 1470 HaeII r_GCGC'y Cuts at: 0 7 1005 1499 1593 Size: 7 998 494 94 Fragments arranged by size: 998 494 94 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 706 708 738 740 1222 1254 1378 1410 Size: 706 2 30 2 482 32 124 32 Cuts at: 1410 1593 Size: 183 Fragments arranged by size: 706 482 183 124 32 32 30 2 2 KasI G'GCGC_C Cuts at: 0 3 1593 Size: 3 1590 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 692 956 1593 Size: 692 264 637 Fragments arranged by size: 692 637 264 MscI TGG'CCA Cuts at: 0 41 1469 1593 Size: 41 1428 124 Fragments arranged by size: 1428 124 41 MslI CAynn'nnrTG Cuts at: 0 212 601 1593 Size: 212 389 992 Fragments arranged by size: 992 389 212 MspA1I CmG'CkG Cuts at: 0 30 307 706 1451 1473 1593 Size: 30 277 399 745 22 120 Fragments arranged by size: 745 399 277 120 30 22 NarI GG'CG_CC Cuts at: 0 4 1593 Size: 4 1589 NcoI C'CATG_G Cuts at: 0 385 1593 Size: 385 1208 NheI G'CTAG_C Cuts at: 0 907 1593 Size: 907 686 PflMI CCAn_nnn'nTGG Cuts at: 0 771 1593 Size: 771 822 PstI C_TGCA'G Cuts at: 0 1557 1593 Size: 1557 36 PvuII CAG'CTG Cuts at: 0 307 706 1593 Size: 307 399 887 Fragments arranged by size: 887 399 307 ScaI AGT'ACT Cuts at: 0 103 1593 Size: 103 1490 SfcI C'TryA_G Cuts at: 0 788 1553 1593 Size: 788 765 40 Fragments arranged by size: 788 765 40 SfoI GGC'GCC Cuts at: 0 5 1593 Size: 5 1588 SgrAI Cr'CCGG_yG Cuts at: 0 600 1593 Size: 600 993 SmaI CCC'GGG Cuts at: 0 1333 1593 Size: 1333 260 SrfI GCCC'GGGC Cuts at: 0 1333 1593 Size: 1333 260 StyI C'CwwG_G Cuts at: 0 194 385 686 1010 1422 1593 Size: 194 191 301 324 412 171 Fragments arranged by size: 412 324 301 194 191 171 TatI w'GTAC_w Cuts at: 0 101 179 404 881 1593 Size: 101 78 225 477 712 Fragments arranged by size: 712 477 225 101 78 XbaI T'CTAG_A Cuts at: 0 297 1593 Size: 297 1296 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 34 1593 Size: 34 1559 XmaI C'CCGG_G Cuts at: 0 1331 1593 Size: 1331 262 XmnI GAAnn'nnTTC Cuts at: 0 169 1593 Size: 169 1424 Enzymes that do cut: AgeI AleI AlwNI AvaI BanI BanII BbeI BbsI BcgI BglII BmrI BmtI BplI BpmI Bpu10I BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrDI BsrFI BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI DrdI EaeI EarI EciI Eco57MI EcoO109I EcoRV HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI NcoI NheI PflMI PstI PvuII ScaI SfcI SfoI SgrAI SmaI SrfI StyI TatI XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BpuEI BsaI BsaAI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrGI BssHII BstAPI BstBI BstZ17I BtgZI DraI DraIII EagI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SmlI SnaBI SpeI SphI SspI StuI SwaI TaqII TstI Tth111I XhoI ZraI