################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccI AflII AflIII AgeI AhdI AocI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BcgI BciVI BfrI BlnI BlpI Bpu1102I BsaAI BsgI BsiWI BsmBI Bsp1407I BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I CpoI DraI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI FspI HindIII HpaI KasI KpnI MfeI MluI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 623 TCCGGA T^CCGGA AclI 504 AACGTT AA^CGTT AfaI 93 GTAC GT^AC Alw44I 675 GTGCAC G^TGCAC ApaI 1735 GGGCCC GGGCC^C ApaLI 675 GTGCAC G^TGCAC AvaI 911 CTCGAG C^[CT]CG[GA]G BamHI 1788 GGATCC G^GATCC BbsI 1483 GAAGAC GAAGAC(2/6) BbvCI 316 CCTCAGC CCTCAGC(-5/-2) BclI 1550 TGATCA T^GATCA BfaI 1205 CTAG C^TAG BpuAI 1483 GAAGAC GAAGAC(2/6) BsaBI 654 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 623 TCCGGA [AT]^CCGG[AT] BseAI 623 TCCGGA T^CCGGA BseRI 1573 GAGGAG GAGGAG(10/8) BsiEI 122 CGATCG CG[GA][CT]^CG BsmI 541 GCATTC GAATGC(1/-1) BsoBI 911 CTCGAG C^[CT]CG[GA]G BspDI 124 ATCGAT AT^CGAT BspEI 623 TCCGGA T^CCGGA BspHI 950 TCATGA T^CATGA BsrDI 261 CATTGC GCAATG(2/0) ClaI 124 ATCGAT AT^CGAT DraIII 1918 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1378 GACTGCGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 717 GAAGAG CTCTTC(1/4) EcoNI 66 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 769 GAATTC G^AATTC FbaI 1550 TGATCA T^GATCA Ksp632I 717 GAAGAG CTCTTC(1/4) MaeI 1205 CTAG C^TAG MamI 654 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 623 TCCGGA T^CCGGA MslI 1227 CACACCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NruI 1368 TCGCGA TCG^CGA PaeR7I 911 CTCGAG C^TCGAG PpuMI 290 GGGTCCC [GA]G^G[AT]CC[CT] Psp1406I 504 AACGTT AA^CGTT PvuI 122 CGATCG CGAT^CG RcaI 950 TCATGA T^CATGA RsaI 93 GTAC GT^AC SacI 1600 GAGCTC GAGCT^C SphI 744 GCATGC GCATG^C XhoI 911 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AcyI 1336 GGCGTC G[GA]^CG[CT]C 1384 GGCGTC BalI 1418 TGGCCA TGG^CCA 2024 TGGCCA BsaHI 1336 GGCGTC G[GA]^CG[CT]C 1384 GGCGTC BsmFI 34 GTCCC GGGAC(10/14) 292 GTCCC BspMI 1654 ACCTGC ACCTGC(4/8) 112 GCAGGT BstBI 58 TTCGAA TT^CGAA 991 TTCGAA EcoT14I 1427 CCAAGG C^C[AT][AT]GG 1865 CCAAGG FauI 1835 CCCGC CCCGC(4/6) 1635 GCGGG HgaI 1337 GCGTC GACGC(5/10) 1385 GCGTC Hin1I 1336 GGCGTC G[GA]^CG[CT]C 1384 GGCGTC HincII 304 GTCAAC GT[CT]^[GA]AC 2005 GTTGAC HindII 304 GTCAAC GT[CT]^[GA]AC 2005 GTTGAC KspI 1280 CCGCGG CCGC^GG 1317 CCGCGG MluNI 1418 TGGCCA TGG^CCA 2024 TGGCCA MscI 1418 TGGCCA TGG^CCA 2024 TGGCCA NspI 744 GCATGC [GA]CATG^[CT] 1694 ACATGC NspV 58 TTCGAA TT^CGAA 991 TTCGAA PflMI 1584 CCACGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1917 CCACACTGTGG PvuII 163 CAGCTG CAG^CTG 1963 CAGCTG SacII 1280 CCGCGG CCGC^GG 1317 CCGCGG SfuI 58 TTCGAA TT^CGAA 991 TTCGAA SmlI 911 CTCGAG C^T[CT][GA]AG 1530 CTTGAG StyI 1427 CCAAGG C^C[AT][AT]GG 1865 CCAAGG Tsp45I 1339 GTCAC ^GT[GC]AC 1828 GTCAC Van91I 1584 CCACGACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1917 CCACACTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AccII AciI AcsI AluI AlwI AlwNI Aor51HI ApoI AspHI AvaII BanII BbvI BcnI BglI BglII BmyI BpmI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eco47III EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII