(Circular) (Six-base) MAPSORT of: pFN21AE6718.seq Check: 5044 from: 1 to: 6751 pFN21AE6718 6751 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2108 3668 2108 Size: 1560 5191 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4561 278 Size: 53 83 186 936 3025 2468 Fragments arranged by size: 3025 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4094 1937 Size: 2157 4594 Acc65I G'GTAC_C Cuts at: 4072 4072 Size: 6751 AclI AA'CG_TT Cuts at: 1563 2510 4878 5251 1563 Size: 947 2368 373 3063 Fragments arranged by size: 3063 2368 947 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2953 3507 3752 4814 6198 6466 1632 Size: 1321 554 245 1062 1384 268 1917 Fragments arranged by size: 1917 1384 1321 1062 554 268 245 AfeI AGC'GCT Cuts at: 2327 2800 2894 2327 Size: 473 94 6184 Fragments arranged by size: 6184 473 94 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6554 AflIII A'CryG_T Cuts at: 1203 5650 6627 1203 Size: 4447 977 1327 Fragments arranged by size: 4447 1327 977 AgeI A'CCGG_T Cuts at: 5582 5582 Size: 6751 AhdI GACnn_n'nnGTC Cuts at: 5480 5480 Size: 6751 AleI CACnn'nnGTG Cuts at: 1166 3236 1166 Size: 2070 4681 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2630 1184 Size: 1446 5305 AlwNI CAG_nnn'CTG Cuts at: 2092 2861 3973 4494 6066 2092 Size: 769 1112 521 1572 2777 Fragments arranged by size: 2777 1572 1112 769 521 ApaI G_GGCC'C Cuts at: 3744 3744 Size: 6751 ApaLI G'TGCA_C Cuts at: 2680 4808 5964 2680 Size: 2128 1156 3467 Fragments arranged by size: 3467 2128 1156 ApoI r'AATT_y Cuts at: 1493 2345 2396 2774 3040 3495 4060 4286 4438 Size: 852 51 378 266 455 565 226 152 Cuts at: 4438 6547 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 852 565 455 378 266 226 152 51 AseI AT'TA_AT Cuts at: 160 1032 5305 160 Size: 872 4273 1606 Fragments arranged by size: 4273 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6751 AvaI C'yCGr_G Cuts at: 1870 1943 2916 4076 1870 Size: 73 973 1160 4545 Fragments arranged by size: 4545 1160 973 73 BamHI G'GATC_C Cuts at: 3793 4081 4448 3793 Size: 288 367 6096 Fragments arranged by size: 6096 367 288 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4072 5527 618 Size: 325 217 627 2285 1455 1842 Fragments arranged by size: 2285 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3609 3676 3744 4070 1391 Size: 2218 67 68 326 4072 Fragments arranged by size: 4072 2218 326 68 67 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3495 928 Size: 971 1596 4184 Fragments arranged by size: 4184 1596 971 BbvCI CC'TCA_GC Cuts at: 2322 2322 Size: 6751 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4942 4976 1903 Size: 34 3005 34 3678 Fragments arranged by size: 3678 3005 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4643 5853 4643 Size: 1210 5541 BclI T'GATC_A Cuts at: 1548 3555 1548 Size: 2007 4744 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2858 3276 3942 Size: 107 122 71 631 605 1186 418 666 Cuts at: 3942 5362 136 Size: 1420 1525 Fragments arranged by size: 1525 1420 1186 666 631 605 418 122 107 71 BglII A'GATC_T Cuts at: 2058 2386 2991 3539 6746 2058 Size: 328 605 548 3207 2063 Fragments arranged by size: 3207 2063 605 548 328 BlpI GC'TnA_GC Cuts at: 4168 4168 Size: 6751 Bme1580I G_kGCm'C Cuts at: 1790 2684 3744 4812 5968 1790 Size: 894 1060 1068 1156 2573 Fragments arranged by size: 2573 1156 1068 1060 894 BmgBI CAC'GTC Cuts at: 1318 2679 1318 Size: 1361 5390 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2295 4482 5440 Size: 416 53 83 399 24 878 2187 958 Cuts at: 5440 442 Size: 1753 Fragments arranged by size: 2187 1753 958 878 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6751 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2114 2708 2855 3596 3623 3923 5411 1239 Size: 875 594 147 741 27 300 1488 2579 Fragments arranged by size: 2579 1488 875 741 594 300 147 27 Bpu10I CC'TnA_GC Cuts at: 2322 3549 3950 2322 Size: 1227 401 5123 Fragments arranged by size: 5123 1227 401 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3556 4876 5741 6039 3556 Size: 1320 865 298 4268 Fragments arranged by size: 4268 1320 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2438 3275 3371 5414 882 Size: 617 939 837 96 2043 2219 Fragments arranged by size: 2219 2043 939 837 617 96 BsaAI yAC'GTr Cuts at: 493 1204 4542 493 Size: 711 3338 2702 Fragments arranged by size: 3338 2702 711 BsaBI GATnn'nnATC Cuts at: 2663 6745 2663 Size: 4082 2669 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3342 3390 4558 4940 Size: 53 83 186 936 1809 48 1168 382 Cuts at: 4940 5575 275 Size: 635 1451 Fragments arranged by size: 1809 1451 1168 936 635 382 186 83 53 48 BsaWI w'CCGG_w Cuts at: 2628 5184 5582 5856 6003 6414 2628 Size: 2556 398 274 147 411 2965 Fragments arranged by size: 2965 2556 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2574 2604 2630 2660 1178 Size: 30 165 30 1171 30 26 30 5269 Fragments arranged by size: 5269 1171 165 30 30 30 30 26 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3593 1186 Size: 2407 4344 BseYI C'CCAG_C Cuts at: 2280 3722 5954 6450 2280 Size: 1442 2232 496 2581 Fragments arranged by size: 2581 2232 1442 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6178 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2130 4201 4962 5990 1540 Size: 275 186 129 2071 761 1028 2301 Fragments arranged by size: 2301 2071 1028 761 275 186 129 BsiHKAI G_wGCw'C Cuts at: 2684 2814 3063 3609 3869 4812 4897 5968 2684 Size: 130 249 546 260 943 85 1071 3467 Fragments arranged by size: 3467 1071 943 546 260 249 130 85 BsmI GAATG_Cn' Cuts at: 2546 4262 4355 2546 Size: 1716 93 4942 Fragments arranged by size: 4942 1716 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2684 2814 3063 3609 3676 3744 3869 Size: 399 894 130 249 546 67 68 125 Cuts at: 3869 4070 4812 4897 5968 1391 Size: 201 742 85 1071 2174 Fragments arranged by size: 2174 1071 894 742 546 399 249 201 130 125 85 68 67 BspEI T'CCGG_A Cuts at: 2628 6414 2628 Size: 3786 2965 BspHI T'CATG_A Cuts at: 2955 4640 6370 2955 Size: 1685 1730 3336 Fragments arranged by size: 3336 1730 1685 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2108 3668 4106 844 Size: 1264 1560 438 3489 Fragments arranged by size: 3489 1560 1264 438 BsrBI CCG'CTC Cuts at: 1394 1823 4638 1394 Size: 429 2815 3507 Fragments arranged by size: 3507 2815 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2265 5246 5420 65 Size: 1183 1017 2981 174 1396 Fragments arranged by size: 2981 1396 1183 1017 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5395 5582 1700 Size: 110 3585 187 2869 Fragments arranged by size: 3585 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6751 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6751 BssSI C'ACGA_G Cuts at: 4811 5823 4811 Size: 1012 5739 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6751 BstBI TT'CG_AA Cuts at: 2064 2997 5555 2064 Size: 933 2558 3260 Fragments arranged by size: 3260 2558 933 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6751 BstXI CCAn_nnnn'nTGG Cuts at: 3355 3664 4027 3355 Size: 309 363 6079 Fragments arranged by size: 6079 363 309 BstYI r'GATC_y Cuts at: 1972 2058 2386 2658 2991 3539 3585 3793 4081 Size: 86 328 272 333 548 46 208 288 Cuts at: 4081 4448 4833 4850 6302 6388 6400 6746 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 548 385 367 346 333 328 288 272 208 86 86 46 17 12 BtgI C'CryG_G Cuts at: 513 1065 2290 2769 3150 3285 3322 3336 3354 Size: 552 1225 479 381 135 37 14 18 Cuts at: 3354 3529 513 Size: 175 3735 Fragments arranged by size: 3735 1225 552 479 381 175 135 37 18 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2434 2833 487 Size: 683 56 1208 399 4405 Fragments arranged by size: 4405 1208 683 399 56 BtsI GCAGTG_nn' Cuts at: 2862 4267 5061 5081 2862 Size: 1405 794 20 4532 Fragments arranged by size: 4532 1405 794 20 ClaI AT'CG_AT Cuts at: 1554 2130 1554 Size: 576 6175 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6716 DraI TTT'AAA Cuts at: 4055 4055 Size: 6751 DraIII CAC_nnn'GTG Cuts at: 3928 3928 Size: 6751 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3389 5758 809 Size: 507 2073 2369 1802 Fragments arranged by size: 2369 2073 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2898 3423 4029 Size: 54 1425 50 275 18 1068 525 606 Cuts at: 4029 4198 5087 6716 8 Size: 169 889 1629 43 Fragments arranged by size: 1629 1425 1068 889 606 525 275 169 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4198 1537 Size: 275 2386 4090 Fragments arranged by size: 4090 2386 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2717 4681 1353 Size: 1364 1964 3423 Fragments arranged by size: 3423 1964 1364 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2728 5338 5712 5858 387 Size: 1064 138 1139 2610 374 146 1280 Fragments arranged by size: 2610 1280 1139 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3607 3607 Size: 6751 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2114 2708 2855 2953 3507 3596 3623 Size: 393 482 594 147 98 554 89 27 Cuts at: 3623 3752 3923 4814 5411 6198 6466 1239 Size: 129 171 891 597 787 268 1524 Fragments arranged by size: 1524 891 787 597 594 554 482 393 268 171 147 129 98 89 27 EcoNI CCTnn'n_nnAGG Cuts at: 2075 2075 Size: 6751 EcoO109I rG'GnC_Cy Cuts at: 2080 2296 3741 2080 Size: 216 1445 5090 Fragments arranged by size: 5090 1445 216 EcoRI G'AATT_C Cuts at: 2774 4060 4438 2774 Size: 1286 378 5087 Fragments arranged by size: 5087 1286 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2077 3010 3100 2077 Size: 933 90 5728 Fragments arranged by size: 5728 933 90 FspI TGC'GCA Cuts at: 5257 5257 Size: 6751 HaeII r_GCGC'y Cuts at: 1678 2329 2802 2896 3305 3330 5898 1678 Size: 651 473 94 409 25 2568 2531 Fragments arranged by size: 2568 2531 651 473 409 94 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2411 2443 2668 2700 2897 Size: 32 135 32 839 32 225 32 197 Cuts at: 2897 2929 3099 3131 5392 5424 5466 5498 1373 Size: 32 170 32 2261 32 42 32 2626 Fragments arranged by size: 2626 2261 839 225 197 170 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2311 4012 4095 4341 669 Size: 988 281 373 1701 83 246 3079 Fragments arranged by size: 3079 1701 988 373 281 246 83 HindIII A'AGCT_T Cuts at: 748 748 Size: 6751 HpaI GTT'AAC Cuts at: 4341 4341 Size: 6751 KpnI G_GTAC'C Cuts at: 4076 4076 Size: 6751 MfeI C'AATT_G Cuts at: 4350 4350 Size: 6751 MluI A'CGCG_T Cuts at: 6627 6627 Size: 6751 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2517 5865 6049 1305 Size: 1212 3348 184 2007 Fragments arranged by size: 3348 2007 1212 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3425 4031 10 Size: 54 1425 343 1593 606 2730 Fragments arranged by size: 2730 1593 1425 606 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3236 4709 5068 5227 518 Size: 648 2070 1473 359 159 2042 Fragments arranged by size: 2070 2042 1473 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2089 2170 3287 3324 3964 3970 4167 4844 Size: 483 81 1117 37 640 6 197 677 Cuts at: 4844 5992 6237 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 1117 677 640 483 245 197 81 37 6 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6199 NdeI CA'TA_TG Cuts at: 387 387 Size: 6751 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6751 NotI GC'GGCC_GC Cuts at: 4198 4198 Size: 6751 NruI TCG'CGA Cuts at: 3375 3375 Size: 6751 NspI r_CATG'y Cuts at: 1132 1229 2753 3703 4109 5654 1132 Size: 97 1524 950 406 1545 2229 Fragments arranged by size: 2229 1545 1524 950 406 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6751 PciI A'CATG_T Cuts at: 5650 5650 Size: 6751 PflMI CCAn_nnn'nTGG Cuts at: 1108 3595 3928 1108 Size: 2487 333 3931 Fragments arranged by size: 3931 2487 333 PfoI T'CCnGG_A Cuts at: 2832 2832 Size: 6751 PmeI GTTT'AAAC Cuts at: 4055 4055 Size: 6751 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4820 4852 6333 6365 4820 Size: 32 1481 32 5206 Fragments arranged by size: 5206 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2296 2296 Size: 6751 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6751 PsiI TTA'TAA Cuts at: 4321 4321 Size: 6751 PspOMI G'GGCC_C Cuts at: 3740 3740 Size: 6751 PstI C_TGCA'G Cuts at: 830 2088 2949 3828 3969 4103 830 Size: 1258 861 879 141 134 3478 Fragments arranged by size: 3478 1258 879 861 141 134 PvuI CG_AT'CG Cuts at: 2001 2130 2001 Size: 129 6622 PvuII CAG'CTG Cuts at: 2170 3970 2170 Size: 1800 4951 SacI G_AGCT'C Cuts at: 3609 3609 Size: 6751 SacII CC_GC'GG Cuts at: 3288 3325 3288 Size: 37 6714 SalI G'TCGA_C Cuts at: 1936 4093 1936 Size: 2157 4594 SanDI GG'GwC_CC Cuts at: 2296 2296 Size: 6751 SbfI CC_TGCA'GG Cuts at: 4103 4103 Size: 6751 ScaI AGT'ACT Cuts at: 4999 6711 4999 Size: 1712 5039 SfcI C'TryA_G Cuts at: 826 1045 2032 2084 2945 3824 3965 4099 5234 Size: 219 987 52 861 879 141 134 1135 Cuts at: 5234 5915 6106 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 987 879 861 681 219 191 141 134 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6751 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6751 SmaI CCC'GGG Cuts at: 1872 4078 1872 Size: 2206 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2916 3535 4855 5756 6018 820 Size: 197 926 973 619 1320 901 262 1553 Fragments arranged by size: 1553 1320 973 926 901 619 262 197 SnaBI TAC'GTA Cuts at: 493 4542 493 Size: 4049 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6751 SphI G_CATG'C Cuts at: 1132 2753 4109 1132 Size: 1621 1356 3774 Fragments arranged by size: 3774 1621 1356 SspI AAT'ATT Cuts at: 5 52 4675 5 Size: 47 4623 2081 Fragments arranged by size: 4623 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6751 StyI C'CwwG_G Cuts at: 513 1065 3432 3870 4190 513 Size: 552 2367 438 320 3074 Fragments arranged by size: 3074 2367 552 438 320 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2303 3056 3395 3507 4778 4795 4948 Size: 1255 723 753 339 112 1271 17 153 Cuts at: 4948 5133 5578 325 Size: 185 445 1498 Fragments arranged by size: 1498 1271 1255 753 723 445 339 185 153 112 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2097 4997 6709 Size: 274 80 33 51 1443 120 2900 1712 Cuts at: 6709 96 Size: 138 Fragments arranged by size: 2900 1712 1443 274 138 120 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6711 6743 6711 Size: 32 6719 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6751 XbaI T'CTAG_A Cuts at: 4087 4087 Size: 6751 XhoI C'TCGA_G Cuts at: 1943 2916 1943 Size: 973 5778 XmaI C'CCGG_G Cuts at: 1870 4076 1870 Size: 2206 4545 XmnI GAAnn'nnTTC Cuts at: 4880 4880 Size: 6751 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4559 276 Size: 53 83 186 936 3025 2468 Fragments arranged by size: 3025 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspXI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI XcmI