(Linear) (Six-base) MAPSORT of: KIEE6718.seq Check: 3955 from: 1 to: 2046 KIEE6718.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 103 1663 2046 Size: 103 1560 383 Fragments arranged by size: 1560 383 103 AclI AA'CG_TT Cuts at: 0 505 2046 Size: 505 1541 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 948 1502 1747 2046 Size: 948 554 245 299 Fragments arranged by size: 948 554 299 245 AfeI AGC'GCT Cuts at: 0 322 795 889 2046 Size: 322 473 94 1157 Fragments arranged by size: 1157 473 322 94 AleI CACnn'nnGTG Cuts at: 0 1231 2046 Size: 1231 815 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 625 2046 Size: 625 1421 AlwNI CAG_nnn'CTG Cuts at: 0 87 856 1968 2046 Size: 87 769 1112 78 Fragments arranged by size: 1112 769 87 78 ApaI G_GGCC'C Cuts at: 0 1739 2046 Size: 1739 307 ApaLI G'TGCA_C Cuts at: 0 675 2046 Size: 675 1371 ApoI r'AATT_y Cuts at: 0 340 391 769 1035 1490 2046 Size: 340 51 378 266 455 556 Fragments arranged by size: 556 455 378 340 266 51 AvaI C'yCGr_G Cuts at: 0 911 2046 Size: 911 1135 BamHI G'GATC_C Cuts at: 0 1788 2046 Size: 1788 258 BanII G_rGCy'C Cuts at: 0 1604 1671 1739 2046 Size: 1604 67 68 307 Fragments arranged by size: 1604 307 68 67 BbsI GAAGACnn'nnnn_ Cuts at: 0 1490 2046 Size: 1490 556 BbvCI CC'TCA_GC Cuts at: 0 317 2046 Size: 317 1729 BclI T'GATC_A Cuts at: 0 1550 2046 Size: 1550 496 BglI GCCn_nnn'nGGC Cuts at: 0 853 1271 1937 2046 Size: 853 418 666 109 Fragments arranged by size: 853 666 418 109 BglII A'GATC_T Cuts at: 0 53 381 986 1534 2046 Size: 53 328 605 548 512 Fragments arranged by size: 605 548 512 328 53 Bme1580I G_kGCm'C Cuts at: 0 679 1739 2046 Size: 679 1060 307 Fragments arranged by size: 1060 679 307 BmgBI CAC'GTC Cuts at: 0 674 2046 Size: 674 1372 BmrI ACTGGGnnnn_n' Cuts at: 0 290 2046 Size: 290 1756 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 703 850 1591 1618 1918 2046 Size: 109 594 147 741 27 300 128 Fragments arranged by size: 741 594 300 147 128 109 27 Bpu10I CC'TnA_GC Cuts at: 0 317 1544 1945 2046 Size: 317 1227 401 101 Fragments arranged by size: 1227 401 317 101 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1551 2046 Size: 1551 495 BsaI GGTCTCn'nnnn_ Cuts at: 0 433 1270 1366 2046 Size: 433 837 96 680 Fragments arranged by size: 837 680 433 96 BsaBI GATnn'nnATC Cuts at: 0 658 2046 Size: 658 1388 BsaHI Gr'CG_yC Cuts at: 0 1337 1385 2046 Size: 1337 48 661 Fragments arranged by size: 1337 661 48 BsaWI w'CCGG_w Cuts at: 0 623 2046 Size: 623 1423 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 569 599 625 655 2046 Size: 569 30 26 30 1391 Fragments arranged by size: 1391 569 30 30 26 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1588 2046 Size: 1588 458 BseYI C'CCAG_C Cuts at: 0 275 1717 2046 Size: 275 1442 329 Fragments arranged by size: 1442 329 275 BsiEI CG_ry'CG Cuts at: 0 125 2046 Size: 125 1921 BsiHKAI G_wGCw'C Cuts at: 0 679 809 1058 1604 1864 2046 Size: 679 130 249 546 260 182 Fragments arranged by size: 679 546 260 249 182 130 BsmI GAATG_Cn' Cuts at: 0 541 2046 Size: 541 1505 Bsp1286I G_dGCh'C Cuts at: 0 679 809 1058 1604 1671 1739 1864 2046 Size: 679 130 249 546 67 68 125 182 Fragments arranged by size: 679 546 249 182 130 125 68 67 BspEI T'CCGG_A Cuts at: 0 623 2046 Size: 623 1423 BspHI T'CATG_A Cuts at: 0 950 2046 Size: 950 1096 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 103 1663 2046 Size: 103 1560 383 Fragments arranged by size: 1560 383 103 BsrDI GCAATG_nn' Cuts at: 0 260 2046 Size: 260 1786 BstBI TT'CG_AA Cuts at: 0 59 992 2046 Size: 59 933 1054 Fragments arranged by size: 1054 933 59 BstXI CCAn_nnnn'nTGG Cuts at: 0 1350 1659 2022 2046 Size: 1350 309 363 24 Fragments arranged by size: 1350 363 309 24 BstYI r'GATC_y Cuts at: 0 53 381 653 986 1534 1580 1788 2046 Size: 53 328 272 333 548 46 208 258 Fragments arranged by size: 548 333 328 272 258 208 53 46 BtgI C'CryG_G Cuts at: 0 285 764 1145 1280 1317 1331 1349 1524 Size: 285 479 381 135 37 14 18 175 Cuts at: 1524 2046 Size: 522 Fragments arranged by size: 522 479 381 285 175 135 37 18 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 429 828 2046 Size: 429 399 1218 Fragments arranged by size: 1218 429 399 BtsI GCAGTG_nn' Cuts at: 0 857 2046 Size: 857 1189 ClaI AT'CG_AT Cuts at: 0 125 2046 Size: 125 1921 DraIII CAC_nnn'GTG Cuts at: 0 1923 2046 Size: 1923 123 DrdI GACnn_nn'nnGTC Cuts at: 0 1384 2046 Size: 1384 662 EaeI y'GGCC_r Cuts at: 0 893 1418 2024 2046 Size: 893 525 606 22 Fragments arranged by size: 893 606 525 22 EarI CTCTTCn'nnn_ Cuts at: 0 712 2046 Size: 712 1334 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 723 2046 Size: 723 1323 EcoICRI GAG'CTC Cuts at: 0 1602 2046 Size: 1602 444 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 703 850 948 1502 1591 1618 1747 Size: 109 594 147 98 554 89 27 129 Cuts at: 1747 1918 2046 Size: 171 128 Fragments arranged by size: 594 554 171 147 129 128 109 98 89 27 EcoNI CCTnn'n_nnAGG Cuts at: 0 70 2046 Size: 70 1976 EcoO109I rG'GnC_Cy Cuts at: 0 75 291 1736 2046 Size: 75 216 1445 310 Fragments arranged by size: 1445 310 216 75 EcoRI G'AATT_C Cuts at: 0 769 2046 Size: 769 1277 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 72 1005 1095 2046 Size: 72 933 90 951 Fragments arranged by size: 951 933 90 72 HaeII r_GCGC'y Cuts at: 0 324 797 891 1300 1325 2046 Size: 324 473 94 409 25 721 Fragments arranged by size: 721 473 409 324 94 25 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 406 438 663 695 892 924 1094 1126 Size: 406 32 225 32 197 32 170 32 Cuts at: 1126 2046 Size: 920 Fragments arranged by size: 920 406 225 197 170 32 32 32 32 HincII GTy'rAC Cuts at: 0 306 2007 2046 Size: 306 1701 39 Fragments arranged by size: 1701 306 39 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 512 2046 Size: 512 1534 MscI TGG'CCA Cuts at: 0 1420 2026 2046 Size: 1420 606 20 Fragments arranged by size: 1420 606 20 MslI CAynn'nnrTG Cuts at: 0 1231 2046 Size: 1231 815 MspA1I CmG'CkG Cuts at: 0 84 165 1282 1319 1959 1965 2046 Size: 84 81 1117 37 640 6 81 Fragments arranged by size: 1117 640 84 81 81 37 6 NruI TCG'CGA Cuts at: 0 1370 2046 Size: 1370 676 NspI r_CATG'y Cuts at: 0 748 1698 2046 Size: 748 950 348 Fragments arranged by size: 950 748 348 PflMI CCAn_nnn'nTGG Cuts at: 0 1590 1923 2046 Size: 1590 333 123 Fragments arranged by size: 1590 333 123 PfoI T'CCnGG_A Cuts at: 0 827 2046 Size: 827 1219 PpuMI rG'GwC_Cy Cuts at: 0 291 2046 Size: 291 1755 PspOMI G'GGCC_C Cuts at: 0 1735 2046 Size: 1735 311 PstI C_TGCA'G Cuts at: 0 83 944 1823 1964 2046 Size: 83 861 879 141 82 Fragments arranged by size: 879 861 141 83 82 PvuI CG_AT'CG Cuts at: 0 125 2046 Size: 125 1921 PvuII CAG'CTG Cuts at: 0 165 1965 2046 Size: 165 1800 81 Fragments arranged by size: 1800 165 81 SacI G_AGCT'C Cuts at: 0 1604 2046 Size: 1604 442 SacII CC_GC'GG Cuts at: 0 1283 1320 2046 Size: 1283 37 726 Fragments arranged by size: 1283 726 37 SanDI GG'GwC_CC Cuts at: 0 291 2046 Size: 291 1755 SfcI C'TryA_G Cuts at: 0 27 79 940 1819 1960 2046 Size: 27 52 861 879 141 86 Fragments arranged by size: 879 861 141 86 52 27 SmlI C'TyrA_G Cuts at: 0 911 1530 2046 Size: 911 619 516 Fragments arranged by size: 911 619 516 SphI G_CATG'C Cuts at: 0 748 2046 Size: 748 1298 StyI C'CwwG_G Cuts at: 0 1427 1865 2046 Size: 1427 438 181 Fragments arranged by size: 1427 438 181 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 298 1051 1390 1502 2046 Size: 298 753 339 112 544 Fragments arranged by size: 753 544 339 298 112 TatI w'GTAC_w Cuts at: 0 92 2046 Size: 92 1954 XhoI C'TCGA_G Cuts at: 0 911 2046 Size: 911 1135 Enzymes that do cut: AarI AclI AcuI AfeI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanII BbsI BbvCI BclI BglI BglII Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BstBI BstXI BstYI BtgI BtgZI BtsI ClaI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII MmeI MscI MslI MspA1I NruI NspI PflMI PfoI PpuMI PspOMI PstI PvuI PvuII SacI SacII SanDI SfcI SmlI SphI StyI TaqII TatI XhoI Enzymes that do not cut: AatII AccI Acc65I AflII AflIII AgeI AhdI AloI AscI AseI AsiSI AvrII BaeI BanI BbeI BcgI BciVI BfrBI BlpI BmtI BplI BsaAI BsgI BsiWI BsmBI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstZ17I Bsu36I CspCI DraI EagI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI XmaI XmnI ZraI