################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## Acc65I AccI AccII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsgI BsiEI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoT22I FbaI FseI FspI HindIII HinfI HpaI KasI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 580 GACGTC GACGT^C AccIII 903 TCCGGA T^CCGGA AcyI 580 GACGTC G[GA]^CG[CT]C AlwNI 835 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 574 CCTGAGG CC^T[ACGT]AGG AspHI 247 GAGCAC G[AT]GC[AT]^C AvaI 25 CCCGGG C^[CT]CG[GA]G AvaII 1002 GGTCC G^G[AT]CC BanI 910 GGCACC G^G[CT][GA]CC BglI 676 GCCGCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 580 GACGTC G[GA]^CG[CT]C BsaWI 903 TCCGGA [AT]^CCGG[AT] BseAI 903 TCCGGA T^CCGGA BseRI 307 GAGGAG GAGGAG(10/8) BsiHKAI 247 GAGCAC G[AT]GC[AT]^C BsiWI 239 CGTACG C^GTACG BsmAI 404 GAGAC GTCTC(1/5) BsmFI 23 GTCCC GGGAC(10/14) BsmI 222 GCATTC GAATGC(1/-1) BsoBI 25 CCCGGG C^[CT]CG[GA]G BspEI 903 TCCGGA T^CCGGA BspMI 587 ACCTGC ACCTGC(4/8) BsrBI 565 CCGCTC CCGCTC(-3/-3) BstAPI 834 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 695 CCAAGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 574 CCTGAGG CC^T[ACGT]AGG DdeI 575 CTGAG C^T[ACGT]AG DraII 1001 AGGTCCT [GA]G^G[ACGT]CC[CT] Eco81I 574 CCTGAGG CC^T[ACGT]AGG EcoNI 197 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1001 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoRV 666 GATATC GAT^ATC HaeII 29 GGCGCT [GA]GCGC^[CT] HgaI 798 GCGTC GACGC(5/10) Hin1I 580 GACGTC G[GA]^CG[CT]C HincII 583 GTCAAC GT[CT]^[GA]AC HindII 583 GTCAAC GT[CT]^[GA]AC MroI 903 TCCGGA T^CCGGA MslI 1029 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NspI 929 ACATGC [GA]CATG^[CT] PpuMI 1001 AGGTCCT [GA]G^G[AT]CC[CT] PstI 292 CTGCAG CTGCA^G PvuII 835 CAGCTG CAG^CTG SfaNI 474 GATGC GCATC(5/9) SfcI 292 CTGCAG C^T[GA][CT]AG SmaI 25 CCCGGG CCC^GGG TaqI 217 TCGA T^CGA TthHB8I 217 TCGA T^CGA XcmI 914 CCATTCTAACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 25 CCCGGG C^CCGGG XmaI 25 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### BalI 125 TGGCCA TGG^CCA 887 TGGCCA BanII 819 GGGCTC G[GA]GC[CT]^C 952 GAGCCC BbsI 234 GAAGAC GAAGAC(2/6) 973 GAAGAC BcnI 25 CCCGG CC^[GC]GG 26 CCGGG BfaI 172 CTAG C^TAG 988 CTAG BpuAI 234 GAAGAC GAAGAC(2/6) 973 GAAGAC BsrDI 63 CATTGC GCAATG(2/0) 860 GCAATG BstYI 317 GGATCT [GA]^GATC[CT] 397 GGATCT Cfr13I 692 GGGCC G^G[ACGT]CC 1002 GGTCC EarI 162 GAAGAG CTCTTC(1/4) 1018 GAAGAG EcoRI 175 GAATTC G^AATTC 358 GAATTC HapII 26 CCGG C^CGG 904 CCGG HpaII 26 CCGG C^CGG 904 CCGG Ksp632I 162 GAAGAG CTCTTC(1/4) 1018 GAAGAG MaeI 172 CTAG C^TAG 988 CTAG MflI 317 GGATCT [GA]^GATC[CT] 397 GGATCT MluNI 125 TGGCCA TGG^CCA 887 TGGCCA MscI 125 TGGCCA TGG^CCA 887 TGGCCA MseI 185 TTAA T^TAA 983 TTAA MspA1I 4 CCGCTG C[AC]G^C[GT]G 835 CAGCTG MspI 26 CCGG C^CGG 904 CCGG NciI 25 CCCGG CC^[GC]GG 26 CCGGG Sau96I 692 GGGCC G^G[ACGT]CC 1002 GGTCC Tru9I 185 TTAA T^TAA 983 TTAA XhoII 317 GGATCT [GA]^GATC[CT] 397 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI BbvI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI DpnII EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HhaI HinP1I HphI ItaI MaeII MaeIII MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI StyI TseI Tsp45I Tsp509I TspRI