(Circular) (Six-base) MAPSORT of: pFN21AE0416.seq Check: 5640 from: 1 to: 5761 pFN21AE0416 5761 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3571 278 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3104 1937 Size: 1167 4594 Acc65I G'GTAC_C Cuts at: 3082 3082 Size: 5761 AclI AA'CG_TT Cuts at: 1563 3888 4261 1563 Size: 2325 373 3063 Fragments arranged by size: 3063 2325 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2276 2450 2591 2918 3824 5208 5476 1632 Size: 644 174 141 327 906 1384 268 1917 Fragments arranged by size: 1917 1384 906 644 327 268 174 141 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5564 AflIII A'CryG_T Cuts at: 1203 2756 4660 5637 1203 Size: 1553 1904 977 1327 Fragments arranged by size: 1904 1553 1327 977 AgeI A'CCGG_T Cuts at: 4592 4592 Size: 5761 AhdI GACnn_n'nnGTC Cuts at: 4490 4490 Size: 5761 AleI CACnn'nnGTG Cuts at: 1166 2060 3038 1166 Size: 894 978 3889 Fragments arranged by size: 3889 978 894 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5761 AlwNI CAG_nnn'CTG Cuts at: 2284 2721 2794 2863 3504 5076 2284 Size: 437 73 69 641 1572 2969 Fragments arranged by size: 2969 1572 641 437 73 69 ApaLI G'TGCA_C Cuts at: 3818 4974 3818 Size: 1156 4605 ApoI r'AATT_y Cuts at: 1493 3070 3296 3448 5557 1493 Size: 1577 226 152 2109 1697 Fragments arranged by size: 2109 1697 1577 226 152 AseI AT'TA_AT Cuts at: 160 1032 4315 160 Size: 872 3283 1606 Fragments arranged by size: 3283 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 AvaI C'yCGr_G Cuts at: 1870 1943 2103 3036 3086 1870 Size: 73 160 933 50 4545 Fragments arranged by size: 4545 933 160 73 50 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3035 3068 3035 Size: 33 5728 BamHI G'GATC_C Cuts at: 3091 3458 3091 Size: 367 5394 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2051 2389 3082 4537 618 Size: 325 217 627 264 338 693 1455 1842 Fragments arranged by size: 1842 1455 693 627 338 325 264 217 BanII G_rGCy'C Cuts at: 1391 2121 3003 3080 1391 Size: 730 882 77 4072 Fragments arranged by size: 4072 882 730 77 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4790 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3952 3986 1903 Size: 34 2015 34 3678 Fragments arranged by size: 3678 2015 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3653 4863 3653 Size: 1210 4551 BclI T'GATC_A Cuts at: 1548 1548 Size: 5761 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4372 136 Size: 107 122 71 631 605 2700 1525 Fragments arranged by size: 2700 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5756 5756 Size: 5761 BlpI GC'TnA_GC Cuts at: 2864 3178 2864 Size: 314 5447 Bme1580I G_kGCm'C Cuts at: 1790 2054 2379 2394 2961 3014 3822 4978 1790 Size: 264 325 15 567 53 808 1156 2573 Fragments arranged by size: 2573 1156 808 567 325 264 53 15 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5761 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3492 4450 442 Size: 416 53 83 399 24 2075 958 1753 Fragments arranged by size: 2075 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5761 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2163 2969 4421 1239 Size: 924 806 1452 2579 Fragments arranged by size: 2579 1452 924 806 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2105 2804 3886 4751 5049 2105 Size: 699 1082 865 298 2817 Fragments arranged by size: 2817 1082 865 699 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2113 2549 4424 882 Size: 617 614 436 1875 2219 Fragments arranged by size: 2219 1875 617 614 436 BsaAI yAC'GTr Cuts at: 493 1204 3552 493 Size: 711 2348 2702 Fragments arranged by size: 2702 2348 711 BsaBI GATnn'nnATC Cuts at: 5755 5755 Size: 5761 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3568 3950 4585 275 Size: 53 83 186 936 2035 382 635 1451 Fragments arranged by size: 2035 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2781 4194 4592 4866 5013 5424 2781 Size: 1413 398 274 147 411 3118 Fragments arranged by size: 3118 1413 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5536 Fragments arranged by size: 5536 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2576 2849 1186 Size: 1390 273 4098 Fragments arranged by size: 4098 1390 273 BseYI C'CCAG_C Cuts at: 2856 4964 5460 2856 Size: 2108 496 3157 Fragments arranged by size: 3157 2108 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2956 1163 Size: 573 1220 3968 Fragments arranged by size: 3968 1220 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3211 3972 5000 1540 Size: 275 186 1210 761 1028 2301 Fragments arranged by size: 2301 1210 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2121 2301 3822 3907 4978 2121 Size: 180 1521 85 1071 2904 Fragments arranged by size: 2904 1521 1071 180 85 BsmI GAATG_Cn' Cuts at: 3272 3365 3272 Size: 93 5668 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2054 2121 2301 2379 2394 2961 3003 Size: 399 264 67 180 78 15 567 42 Cuts at: 3003 3014 3080 3822 3907 4978 1391 Size: 11 66 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 567 399 264 180 85 78 67 66 42 15 11 BspEI T'CCGG_A Cuts at: 2781 5424 2781 Size: 2643 3118 BspHI T'CATG_A Cuts at: 3650 5380 3650 Size: 1730 4031 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3116 844 Size: 2272 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2013 2536 3648 1394 Size: 429 190 523 1112 3507 Fragments arranged by size: 3507 1112 523 429 190 BsrDI GCAATG_nn' Cuts at: 65 1248 4256 4430 65 Size: 1183 3008 174 1396 Fragments arranged by size: 3008 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2091 4405 4592 1700 Size: 110 281 2314 187 2869 Fragments arranged by size: 2869 2314 281 187 110 BsrGI T'GTAC_A Cuts at: 96 3051 96 Size: 2955 2806 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5761 BssSI C'ACGA_G Cuts at: 2396 3821 4833 2396 Size: 1425 1012 3324 Fragments arranged by size: 3324 1425 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2794 2979 794 Size: 2000 185 3576 Fragments arranged by size: 3576 2000 185 BstBI TT'CG_AA Cuts at: 4565 4565 Size: 5761 BstEII G'GTnAC_C Cuts at: 1184 2065 1184 Size: 881 4880 BstXI CCAn_nnnn'nTGG Cuts at: 2062 2402 2062 Size: 340 5421 BstYI r'GATC_y Cuts at: 1972 2449 3091 3458 3843 3860 5312 5398 5410 Size: 477 642 367 385 17 1452 86 12 Cuts at: 5410 5756 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 642 477 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2880 2880 Size: 5761 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4270 Fragments arranged by size: 4270 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5022 Fragments arranged by size: 5022 683 56 BtsI GCAGTG_nn' Cuts at: 3277 4071 4091 3277 Size: 794 20 4947 Fragments arranged by size: 4947 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5761 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5726 DraI TTT'AAA Cuts at: 2157 3065 2157 Size: 908 4853 DraIII CAC_nnn'GTG Cuts at: 2979 2979 Size: 5761 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4768 809 Size: 507 3452 1802 Fragments arranged by size: 3452 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2515 3208 4097 Size: 54 1425 50 275 18 685 693 889 Cuts at: 4097 5726 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 693 685 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3208 1537 Size: 275 1396 4090 Fragments arranged by size: 4090 1396 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2774 2894 3691 1353 Size: 1421 120 797 3423 Fragments arranged by size: 3423 1421 797 120 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2618 4348 4722 4868 387 Size: 1064 138 1029 1730 374 146 1280 Fragments arranged by size: 1730 1280 1064 1029 374 146 138 EcoICRI GAG'CTC Cuts at: 2119 2119 Size: 5761 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2163 2276 2450 2591 2918 2969 3824 Size: 393 531 113 174 141 327 51 855 Cuts at: 3824 4421 5208 5476 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 855 787 597 531 393 327 268 174 141 113 51 EcoRI G'AATT_C Cuts at: 3070 3448 3070 Size: 378 5383 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2255 2255 Size: 5761 FspI TGC'GCA Cuts at: 4267 4267 Size: 5761 HaeII r_GCGC'y Cuts at: 1678 2738 2812 2907 4908 1678 Size: 1060 74 95 2001 2531 Fragments arranged by size: 2531 2001 1060 95 74 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2341 2373 2606 2638 3015 Size: 32 135 32 769 32 233 32 377 Cuts at: 3015 3047 4402 4434 4476 4508 1373 Size: 32 1355 32 42 32 2626 Fragments arranged by size: 2626 1355 769 377 233 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3105 3351 669 Size: 988 281 1167 246 3079 Fragments arranged by size: 3079 1167 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5761 HpaI GTT'AAC Cuts at: 3351 3351 Size: 5761 KpnI G_GTAC'C Cuts at: 3086 3086 Size: 5761 MfeI C'AATT_G Cuts at: 3360 3360 Size: 5761 MluI A'CGCG_T Cuts at: 5637 5637 Size: 5761 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4875 5059 1305 Size: 3570 184 2007 Fragments arranged by size: 3570 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3939 Fragments arranged by size: 3939 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2060 2145 2400 3038 3719 4078 4237 Size: 648 894 85 255 638 681 359 159 Cuts at: 4237 518 Size: 2042 Fragments arranged by size: 2042 894 681 648 638 359 255 159 85 MspA1I CmG'CkG Cuts at: 1606 2281 2529 2800 2860 3177 3854 5002 5247 Size: 675 248 271 60 317 677 1148 245 Cuts at: 5247 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 675 317 271 248 245 60 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4270 Fragments arranged by size: 4270 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5761 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5761 NotI GC'GGCC_GC Cuts at: 3208 3208 Size: 5761 NspI r_CATG'y Cuts at: 1132 1229 2656 2760 3119 4664 1132 Size: 97 1427 104 359 1545 2229 Fragments arranged by size: 2229 1545 1427 359 104 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5761 PciI A'CATG_T Cuts at: 2756 4660 2756 Size: 1904 3857 PflMI CCAn_nnn'nTGG Cuts at: 1108 2254 1108 Size: 1146 4615 PfoI T'CCnGG_A Cuts at: 2179 2610 2841 2179 Size: 431 231 5099 Fragments arranged by size: 5099 431 231 PmeI GTTT'AAAC Cuts at: 3065 3065 Size: 5761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3830 3862 5343 5375 3830 Size: 32 1481 32 4216 Fragments arranged by size: 4216 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5761 PsiI TTA'TAA Cuts at: 3331 3331 Size: 5761 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2462 2494 2462 Size: 32 5729 PstI C_TGCA'G Cuts at: 830 2637 2697 2799 3023 3113 830 Size: 1807 60 102 224 90 3478 Fragments arranged by size: 3478 1807 224 102 90 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5761 PvuII CAG'CTG Cuts at: 2860 2860 Size: 5761 SacI G_AGCT'C Cuts at: 2121 2121 Size: 5761 SalI G'TCGA_C Cuts at: 1936 3103 1936 Size: 1167 4594 SapI GCTCTTCn'nnn_ Cuts at: 2894 2894 Size: 5761 SbfI CC_TGCA'GG Cuts at: 3113 3113 Size: 5761 ScaI AGT'ACT Cuts at: 2660 4009 5721 2660 Size: 1349 1712 2700 Fragments arranged by size: 2700 1712 1349 SexAI A'CCwGG_T Cuts at: 2385 2385 Size: 5761 SfcI C'TryA_G Cuts at: 826 1045 2633 2693 2795 3019 3109 4244 4925 Size: 219 1588 60 102 224 90 1135 681 Cuts at: 4925 5116 826 Size: 191 1471 Fragments arranged by size: 1588 1471 1135 681 224 219 191 102 90 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5761 SmaI CCC'GGG Cuts at: 1872 2105 3038 3088 1872 Size: 233 933 50 4545 Fragments arranged by size: 4545 933 233 50 SmlI C'TyrA_G Cuts at: 820 1017 1943 2120 2819 3865 4766 5028 820 Size: 197 926 177 699 1046 901 262 1553 Fragments arranged by size: 1553 1046 926 901 699 262 197 177 SnaBI TAC'GTA Cuts at: 493 3552 493 Size: 3059 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5761 SphI G_CATG'C Cuts at: 1132 2656 3119 1132 Size: 1524 463 3774 Fragments arranged by size: 3774 1524 463 SspI AAT'ATT Cuts at: 5 52 3685 5 Size: 47 3633 2081 Fragments arranged by size: 3633 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5761 StyI C'CwwG_G Cuts at: 513 1065 2004 3200 513 Size: 552 939 1196 3074 Fragments arranged by size: 3074 1196 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2135 2339 2573 3788 3805 3958 4143 Size: 1255 555 204 234 1215 17 153 185 Cuts at: 4143 4588 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1215 555 445 234 204 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2079 2658 3051 Size: 274 80 33 51 1443 102 579 393 Cuts at: 3051 4007 5719 96 Size: 956 1712 138 Fragments arranged by size: 1712 1443 956 579 393 274 138 102 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2175 2207 2951 2983 5721 5753 2175 Size: 32 744 32 2738 32 2183 Fragments arranged by size: 2738 2183 744 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5761 XbaI T'CTAG_A Cuts at: 2458 3097 2458 Size: 639 5122 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5761 XmaI C'CCGG_G Cuts at: 1870 2103 3036 3086 1870 Size: 233 933 50 4545 Fragments arranged by size: 4545 933 233 50 XmnI GAAnn'nnTTC Cuts at: 3890 3890 Size: 5761 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3569 276 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI