(Circular) (Six-base) MAPSORT of: pFN21AE0004.seq Check: 1265 from: 1 to: 6163 pFN21AE0004 6163 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2187 2187 Size: 6163 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3973 278 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3506 1937 Size: 1569 4594 Acc65I G'GTAC_C Cuts at: 3484 3484 Size: 6163 AclI AA'CG_TT Cuts at: 1563 4290 4663 1563 Size: 2727 373 3063 Fragments arranged by size: 3063 2727 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2270 2478 3227 3298 3395 4226 5610 5878 Size: 638 208 749 71 97 831 1384 268 Cuts at: 5878 1632 Size: 1917 Fragments arranged by size: 1917 1384 831 749 638 268 208 97 71 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5966 AflIII A'CryG_T Cuts at: 1203 2307 2735 3294 5062 6039 1203 Size: 1104 428 559 1768 977 1327 Fragments arranged by size: 1768 1327 1104 977 559 428 AgeI A'CCGG_T Cuts at: 4994 4994 Size: 6163 AhdI GACnn_n'nnGTC Cuts at: 4892 4892 Size: 6163 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6163 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6163 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2447 2479 2447 Size: 32 6131 AlwNI CAG_nnn'CTG Cuts at: 3906 5478 3906 Size: 1572 4591 ApaI G_GGCC'C Cuts at: 3275 3275 Size: 6163 ApaLI G'TGCA_C Cuts at: 4220 5376 4220 Size: 1156 5007 ApoI r'AATT_y Cuts at: 1493 3472 3698 3850 5959 1493 Size: 1979 226 152 2109 1697 Fragments arranged by size: 2109 1979 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4717 160 Size: 872 3685 1606 Fragments arranged by size: 3685 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 AvaI C'yCGr_G Cuts at: 1870 1943 2186 2473 3488 1870 Size: 73 243 287 1015 4545 Fragments arranged by size: 4545 1015 287 243 73 AvrII C'CTAG_G Cuts at: 3082 3082 Size: 6163 BamHI G'GATC_C Cuts at: 2799 3493 3860 2799 Size: 694 367 5102 Fragments arranged by size: 5102 694 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2933 2945 3484 4939 618 Size: 325 217 627 1146 12 539 1455 1842 Fragments arranged by size: 1842 1455 1146 627 539 325 217 12 BanII G_rGCy'C Cuts at: 1391 2334 3275 3330 3407 3482 1391 Size: 943 941 55 77 75 4072 Fragments arranged by size: 4072 943 941 77 75 55 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5192 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4354 4388 1903 Size: 34 2417 34 3678 Fragments arranged by size: 3678 2417 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4055 5265 4055 Size: 1210 4953 BclI T'GATC_A Cuts at: 1548 2575 2865 1548 Size: 1027 290 4846 Fragments arranged by size: 4846 1027 290 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4774 136 Size: 107 122 71 631 605 3102 1525 Fragments arranged by size: 3102 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6158 6158 Size: 6163 BlpI GC'TnA_GC Cuts at: 3580 3580 Size: 6163 Bme1580I G_kGCm'C Cuts at: 1790 2948 3275 4224 5380 1790 Size: 1158 327 949 1156 2573 Fragments arranged by size: 2573 1158 1156 949 327 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6163 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2101 2337 3894 Size: 416 53 83 399 24 684 236 1557 Cuts at: 3894 4852 442 Size: 958 1753 Fragments arranged by size: 1753 1557 958 684 416 399 236 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6163 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2279 2660 2855 3314 4823 1239 Size: 1040 381 195 459 1509 2579 Fragments arranged by size: 2579 1509 1040 459 381 195 Bpu10I CC'TnA_GC Cuts at: 3401 3401 Size: 6163 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4288 5153 5451 4288 Size: 865 298 5000 Fragments arranged by size: 5000 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4826 882 Size: 617 3327 2219 Fragments arranged by size: 3327 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2310 3954 493 Size: 711 1106 1644 2702 Fragments arranged by size: 2702 1644 1106 711 BsaBI GATnn'nnATC Cuts at: 6157 6157 Size: 6163 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2627 3970 4352 4987 Size: 53 83 186 936 1094 1343 382 635 Cuts at: 4987 275 Size: 1451 Fragments arranged by size: 1451 1343 1094 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2460 3115 4596 4994 5268 5415 5826 2460 Size: 655 1481 398 274 147 411 2797 Fragments arranged by size: 2797 1481 655 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2205 2235 2541 2571 1178 Size: 30 165 30 802 30 306 30 4770 Fragments arranged by size: 4770 802 306 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2088 2436 1186 Size: 902 348 4913 Fragments arranged by size: 4913 902 348 BseYI C'CCAG_C Cuts at: 3190 5366 5862 3190 Size: 2176 496 3491 Fragments arranged by size: 3491 2176 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2555 1163 Size: 573 819 4771 Fragments arranged by size: 4771 819 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3613 4374 5402 1540 Size: 275 186 1612 761 1028 2301 Fragments arranged by size: 2301 1612 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2193 4224 4309 5380 2193 Size: 2031 85 1071 2976 Fragments arranged by size: 2976 2031 1071 85 BsmI GAATG_Cn' Cuts at: 2858 3674 3767 2858 Size: 816 93 5254 Fragments arranged by size: 5254 816 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2193 2334 2948 3275 3330 3407 3482 Size: 399 403 141 614 327 55 77 75 Cuts at: 3482 4224 4309 5380 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 614 403 399 327 141 85 77 75 55 BspEI T'CCGG_A Cuts at: 5826 5826 Size: 6163 BspHI T'CATG_A Cuts at: 4052 5782 4052 Size: 1730 4433 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2187 3372 3518 844 Size: 1343 1185 146 3489 Fragments arranged by size: 3489 1343 1185 146 BsrBI CCG'CTC Cuts at: 1394 1823 4050 1394 Size: 429 2227 3507 Fragments arranged by size: 3507 2227 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2363 4658 4832 65 Size: 1183 1115 2295 174 1396 Fragments arranged by size: 2295 1396 1183 1115 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3227 4807 4994 1700 Size: 110 1417 1580 187 2869 Fragments arranged by size: 2869 1580 1417 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6163 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6163 BssSI C'ACGA_G Cuts at: 4223 5235 4223 Size: 1012 5151 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6163 BstBI TT'CG_AA Cuts at: 4967 4967 Size: 6163 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6163 BstYI r'GATC_y Cuts at: 1972 2799 3441 3493 3860 4245 4262 5714 5800 Size: 827 642 52 367 385 17 1452 86 Cuts at: 5800 5812 6158 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 827 642 385 367 346 86 52 17 12 Bsu36I CC'TnA_GG Cuts at: 3182 3182 Size: 6163 BtgI C'CryG_G Cuts at: 513 1065 2514 513 Size: 552 1449 4162 Fragments arranged by size: 4162 1449 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5424 Fragments arranged by size: 5424 683 56 BtsI GCAGTG_nn' Cuts at: 2992 3679 4473 4493 2992 Size: 687 794 20 4662 Fragments arranged by size: 4662 794 687 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6163 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6128 DraI TTT'AAA Cuts at: 3467 3467 Size: 6163 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5170 809 Size: 507 3854 1802 Fragments arranged by size: 3854 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2430 3610 4499 Size: 54 1425 50 275 18 600 1180 889 Cuts at: 4499 6128 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1180 889 600 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3610 1537 Size: 275 1798 4090 Fragments arranged by size: 4090 1798 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2752 4093 1353 Size: 1399 1341 3423 Fragments arranged by size: 3423 1399 1341 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4750 5124 5270 387 Size: 1064 138 3161 374 146 1280 Fragments arranged by size: 3161 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2270 2279 2478 2660 2855 3227 3298 Size: 393 638 9 199 182 195 372 71 Cuts at: 3298 3314 3395 4226 4823 5610 5878 1239 Size: 16 81 831 597 787 268 1524 Fragments arranged by size: 1524 831 787 638 597 393 372 268 199 195 182 81 71 16 9 EcoO109I rG'GnC_Cy Cuts at: 2041 2148 3187 3272 3337 2041 Size: 107 1039 85 65 4867 Fragments arranged by size: 4867 1039 107 85 65 EcoRI G'AATT_C Cuts at: 3472 3850 3472 Size: 378 5785 FspI TGC'GCA Cuts at: 2892 4669 2892 Size: 1777 4386 HaeII r_GCGC'y Cuts at: 1678 3222 5310 1678 Size: 1544 2088 2531 Fragments arranged by size: 2531 2088 1544 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3426 3458 4804 4836 4878 Size: 32 135 32 1854 32 1346 32 42 Cuts at: 4878 4910 1373 Size: 32 2626 Fragments arranged by size: 2626 1854 1346 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2766 3292 3507 3753 669 Size: 988 281 828 526 215 246 3079 Fragments arranged by size: 3079 988 828 526 281 246 215 HindIII A'AGCT_T Cuts at: 748 748 Size: 6163 HpaI GTT'AAC Cuts at: 3753 3753 Size: 6163 KpnI G_GTAC'C Cuts at: 3488 3488 Size: 6163 MfeI C'AATT_G Cuts at: 3762 3762 Size: 6163 MluI A'CGCG_T Cuts at: 6039 6039 Size: 6163 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2896 5277 5461 1305 Size: 1591 2381 184 2007 Fragments arranged by size: 2381 2007 1591 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4341 Fragments arranged by size: 4341 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2196 4121 4480 4639 518 Size: 648 1030 1925 359 159 2042 Fragments arranged by size: 2042 1925 1030 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3579 4256 5404 5649 1606 Size: 1973 677 1148 245 2120 Fragments arranged by size: 2120 1973 1148 677 245 NaeI GCC'GGC Cuts at: 3229 3229 Size: 6163 NcoI C'CATG_G Cuts at: 513 1065 2514 513 Size: 552 1449 4162 Fragments arranged by size: 4162 1449 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6163 NgoMIV G'CCGG_C Cuts at: 3227 3227 Size: 6163 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6163 NotI GC'GGCC_GC Cuts at: 3610 3610 Size: 6163 NspI r_CATG'y Cuts at: 1132 1229 2764 3298 3521 5066 1132 Size: 97 1535 534 223 1545 2229 Fragments arranged by size: 2229 1545 1535 534 223 97 PasI CC'CwG_GG Cuts at: 1348 3001 1348 Size: 1653 4510 PciI A'CATG_T Cuts at: 3294 5062 3294 Size: 1768 4395 PflMI CCAn_nnn'nTGG Cuts at: 1108 2704 2848 1108 Size: 1596 144 4423 Fragments arranged by size: 4423 1596 144 PfoI T'CCnGG_A Cuts at: 3153 3444 3153 Size: 291 5872 PmeI GTTT'AAAC Cuts at: 3467 3467 Size: 6163 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4232 4264 5745 5777 4232 Size: 32 1481 32 4618 Fragments arranged by size: 4618 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2148 3187 2148 Size: 1039 5124 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6163 PsiI TTA'TAA Cuts at: 3733 3733 Size: 6163 PspOMI G'GGCC_C Cuts at: 3271 3271 Size: 6163 PstI C_TGCA'G Cuts at: 830 2512 2943 3515 830 Size: 1682 431 572 3478 Fragments arranged by size: 3478 1682 572 431 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6163 SalI G'TCGA_C Cuts at: 1936 3505 1936 Size: 1569 4594 SanDI GG'GwC_CC Cuts at: 3187 3187 Size: 6163 SbfI CC_TGCA'GG Cuts at: 2943 3515 2943 Size: 572 5591 ScaI AGT'ACT Cuts at: 4411 6123 4411 Size: 1712 4451 SfcI C'TryA_G Cuts at: 826 1045 2508 2939 3357 3511 4646 5327 5518 Size: 219 1463 431 418 154 1135 681 191 Cuts at: 5518 826 Size: 1471 Fragments arranged by size: 1471 1463 1135 681 431 418 219 191 154 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6163 SmaI CCC'GGG Cuts at: 1872 3490 1872 Size: 1618 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4267 5168 5430 820 Size: 197 926 2324 901 262 1553 Fragments arranged by size: 2324 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3954 493 Size: 3461 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6163 SphI G_CATG'C Cuts at: 1132 3521 1132 Size: 2389 3774 SspI AAT'ATT Cuts at: 5 52 4087 5 Size: 47 4035 2081 Fragments arranged by size: 4035 2081 47 StuI AGG'CCT Cuts at: 1517 2321 3012 1517 Size: 804 691 4668 Fragments arranged by size: 4668 804 691 StyI C'CwwG_G Cuts at: 513 1065 2514 2703 3082 3602 513 Size: 552 1449 189 379 520 3074 Fragments arranged by size: 3074 1449 552 520 379 189 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4190 4207 4360 4545 4990 325 Size: 1255 2610 17 153 185 445 1498 Fragments arranged by size: 2610 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3297 4409 6121 Size: 274 80 33 51 1443 1320 1112 1712 Cuts at: 6121 96 Size: 138 Fragments arranged by size: 1712 1443 1320 1112 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2205 2237 3149 3181 6123 6155 2205 Size: 32 912 32 2942 32 2213 Fragments arranged by size: 2942 2213 912 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6163 XbaI T'CTAG_A Cuts at: 3499 3499 Size: 6163 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2956 3338 2956 Size: 382 5781 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6163 XmaI C'CCGG_G Cuts at: 1870 3488 1870 Size: 1618 4545 XmnI GAAnn'nnTTC Cuts at: 4292 4292 Size: 6163 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3971 276 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI