Restriction map for
pFN21AE0116
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp700I AspEI
AspI AviII BamHI BbeI BbrPI
BbvCI BciVI BclI BfrI BglII
BlpI Bpu1102I BsaAI BsaBI BsaI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoO65I FbaI FseI FspI HincII
HindII HpaI KasI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI TspRI Tth111I XbaI XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
Acc65I 608 GGTACC G^GTACC
AccII 11 CGCG CG^CG
AcyI 958 GACGCC G[GA]^CG[CT]C
Alw44I 37 GTGCAC G^TGCAC
AlwNI 327 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 475 CCTGAGG CC^T[ACGT]AGG
ApaLI 37 GTGCAC G^TGCAC
Asp718I 608 GGTACC G^GTACC
AvrII 381 CCTAGG C^CTAGG
BalI 539 TGGCCA TGG^CCA
BanI 608 GGTACC G^G[CT][GA]CC
BanII 973 GAGCTC G[GA]GC[CT]^C
BcgI 1136 CGAAGCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 381 CCTAGG C^CTAGG
BsaHI 958 GACGCC G[GA]^CG[CT]C
BsaWI 1025 ACCGGA [AT]^CCGG[AT]
BsgI 526 GTGCAG GTGCAG(16/14)
BsmBI 718 GAGACG CGTCTC(1/5)
Bsp1407I 461 TGTACA T^GTACA
BsrFI 605 GCCGGT [GA]^CCGG[CT]
BsrGI 461 TGTACA T^GTACA
BssSI 274 CACGAG CACGAG(-5/-1)
BstAPI 528 GCAGGACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 11 CGCG CG^CG
BstXI 168 CCACTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 860 AGATCC [GA]^GATC[CT]
Bsu36I 475 CCTGAGG CC^T[ACGT]AGG
CfoI 702 GCGC GCG^C
Cfr10I 605 GCCGGT [GA]^CCGG[CT]
DsaI 155 CCATGG C^C[GA][CT]GG
EarI 297 GAAGAG CTCTTC(1/4)
Eco81I 475 CCTGAGG CC^T[ACGT]AGG
EcoNI 828 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 930 GAATTC G^AATTC
EcoRV 1116 GATATC GAT^ATC
EcoT22I 1018 ATGCAT ATGCA^T
HaeII 701 GGCGCT [GA]GCGC^[CT]
HhaI 702 GCGC GCG^C
Hin1I 958 GACGCC G[GA]^CG[CT]C
HinP1I 702 GCGC G^CGC
HphI 94 GGTGA GGTGA(8/7)
KpnI 608 GGTACC GGTAC^C
Ksp632I 297 GAAGAG CTCTTC(1/4)
MaeII 35 ACGT A^CGT
MaeIII 595 GTCAC ^GT[ACGT]AC
MflI 860 AGATCC [GA]^GATC[CT]
MluNI 539 TGGCCA TGG^CCA
MscI 539 TGGCCA TGG^CCA
MvnI 11 CGCG CG^CG
NcoI 155 CCATGG C^CATGG
NsiI 1018 ATGCAT ATGCA^T
NspI 878 ACATGC [GA]CATG^[CT]
PflMI 864 CCACCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 694 CTGCAG CTGCA^G
SacI 973 GAGCTC GAGCT^C
SspBI 461 TGTACA T^GTACA
SspI 283 AATATT AAT^ATT
StuI 731 AGGCCT AGG^CCT
TaqI 399 TCGA T^CGA
Tsp45I 595 GTCAC ^GT[GC]AC
TthHB8I 399 TCGA T^CGA
Van91I 864 CCACCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 860 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 930 GAATTC [GA]^AATT[CT]
1008 GAATTT
AlwI 861 GATCC GGATC(4/5)
945 GATCC
ApoI 930 GAATTC [GA]^AATT[CT]
1008 GAATTT
AspHI 37 GTGCAC G[AT]GC[AT]^C
973 GAGCTC
AvaI 1082 CCCGGG C^[CT]CG[GA]G
1101 CCCGGG
AvaII 48 GGACC G^G[AT]CC
306 GGTCC
BbsI 144 GAAGAC GAAGAC(2/6)
519 GAAGAC
BglI 127 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
733 GCCTTACTGGC
BmyI 37 GTGCAC G[AGT]GC[ACT]^C
973 GAGCTC
BpuAI 144 GAAGAC GAAGAC(2/6)
519 GAAGAC
BseRI 223 GAGGAG GAGGAG(10/8)
652 GAGGAG
BsiHKAI 37 GTGCAC G[AT]GC[AT]^C
973 GAGCTC
BsmAI 260 GAGAC GTCTC(1/5)
718 GAGAC
BsoBI 1082 CCCGGG C^[CT]CG[GA]G
1101 CCCGGG
Bsp1286I 37 GTGCAC G[AGT]GC[ACT]^C
973 GAGCTC
EaeI 539 TGGCCA [CT]^GGCC[GA]
842 TGGCCG
EcoT14I 155 CCATGG C^C[AT][AT]GG
381 CCTAGG
HgaI 9 GACGC GACGC(5/10)
958 GACGC
HindIII 367 AAGCTT A^AGCTT
544 AAGCTT
MseI 233 TTAA T^TAA
287 TTAA
NlaIV 377 GGAACC GG[ACGT]^[ACGT]CC
608 GGTACC
PvuII 173 CAGCTG CAG^CTG
292 CAGCTG
SfaNI 1017 GATGC GCATC(5/9)
726 GCATC
SfcI 337 CTACAG C^T[GA][CT]AG
694 CTGCAG
SmaI 1082 CCCGGG CCC^GGG
1101 CCCGGG
StyI 155 CCATGG C^C[AT][AT]GG
381 CCTAGG
TfiI 270 GATTC G^A[AT]TC
388 GATTC
Tru9I 233 TTAA T^TAA
287 TTAA
Tsp509I 931 AATT ^AATT
1009 AATT
XmaCI 1082 CCCGGG C^CCGGG
1101 CCCGGG
XmaI 1082 CCCGGG C^CCGGG
1101 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI BbvI BcnI
BfaI BpmI BsaJI BsiYI BslI
BsmFI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI MaeI MboI
MboII MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
PleI RsaI Sau3AI Sau96I ScrFI
SmlI TseI