(Circular) (Six-base) MAPSORT of: pFN21AE0116.seq Check: 8794 from: 1 to: 5875 pFN21AE0116 5875 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3685 278 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3218 1937 Size: 1281 4594 Acc65I G'GTAC_C Cuts at: 2613 3196 2613 Size: 583 5292 AclI AA'CG_TT Cuts at: 1563 4002 4375 1563 Size: 2439 373 3063 Fragments arranged by size: 3063 2439 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2181 2321 2537 2633 3058 3938 5322 5590 Size: 549 140 216 96 425 880 1384 268 Cuts at: 5590 1632 Size: 1917 Fragments arranged by size: 1917 1384 880 549 425 268 216 140 96 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5678 AflIII A'CryG_T Cuts at: 1203 4774 5751 1203 Size: 3571 977 1327 Fragments arranged by size: 3571 1327 977 AgeI A'CCGG_T Cuts at: 4706 4706 Size: 5875 AhdI GACnn_n'nnGTC Cuts at: 4604 4604 Size: 5875 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5875 AlwNI CAG_nnn'CTG Cuts at: 2337 3618 5190 2337 Size: 1281 1572 3022 Fragments arranged by size: 3022 1572 1281 ApaLI G'TGCA_C Cuts at: 2042 3932 5088 2042 Size: 1890 1156 2829 Fragments arranged by size: 2829 1890 1156 ApoI r'AATT_y Cuts at: 1493 2935 3013 3184 3410 3562 5671 1493 Size: 1442 78 171 226 152 2109 1697 Fragments arranged by size: 2109 1697 1442 226 171 152 78 AseI AT'TA_AT Cuts at: 160 1032 4429 160 Size: 872 3397 1606 Fragments arranged by size: 3397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 AvaI C'yCGr_G Cuts at: 1870 1943 3087 3106 3200 1870 Size: 73 1144 19 94 4545 Fragments arranged by size: 4545 1144 94 73 19 AvrII C'CTAG_G Cuts at: 2386 2386 Size: 5875 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2011 2044 2011 Size: 33 5842 BamHI G'GATC_C Cuts at: 3205 3572 3205 Size: 367 5508 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2613 3196 4651 618 Size: 325 217 627 826 583 1455 1842 Fragments arranged by size: 1842 1455 826 627 583 325 217 BanII G_rGCy'C Cuts at: 1391 2982 3194 1391 Size: 1591 212 4072 Fragments arranged by size: 4072 1591 212 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2156 2531 928 Size: 971 257 375 4272 Fragments arranged by size: 4272 971 375 257 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3130 3164 4066 4100 1903 Size: 34 1193 34 902 34 3678 Fragments arranged by size: 3678 1193 902 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3767 4977 3767 Size: 1210 4665 BclI T'GATC_A Cuts at: 1548 1548 Size: 5875 BfrBI ATG'CAT Cuts at: 3025 3025 Size: 5875 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2138 2744 4486 Size: 107 122 71 631 605 466 606 1742 Cuts at: 4486 136 Size: 1525 Fragments arranged by size: 1742 1525 631 606 605 466 122 107 71 BglII A'GATC_T Cuts at: 5870 5870 Size: 5875 BlpI GC'TnA_GC Cuts at: 3292 3292 Size: 5875 Bme1580I G_kGCm'C Cuts at: 1790 2046 3936 5092 1790 Size: 256 1890 1156 2573 Fragments arranged by size: 2573 1890 1156 256 BmgBI CAC'GTC Cuts at: 1318 2041 1318 Size: 723 5152 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3606 4564 442 Size: 416 53 83 399 24 2189 958 1753 Fragments arranged by size: 2189 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5875 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2414 2414 Size: 5875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2078 2283 2915 3023 4535 1239 Size: 839 205 632 108 1512 2579 Fragments arranged by size: 2579 1512 839 632 205 108 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2539 2873 2966 4000 4865 5163 2539 Size: 334 93 1034 865 298 3251 Fragments arranged by size: 3251 1034 865 334 298 93 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4538 882 Size: 617 3039 2219 Fragments arranged by size: 3039 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3666 493 Size: 711 2462 2702 Fragments arranged by size: 2702 2462 711 BsaBI GATnn'nnATC Cuts at: 5869 5869 Size: 5875 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2964 3682 4064 4699 Size: 53 83 186 936 1431 718 382 635 Cuts at: 4699 275 Size: 1451 Fragments arranged by size: 1451 1431 936 718 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3030 4308 4706 4980 5127 5538 3030 Size: 1278 398 274 147 411 3367 Fragments arranged by size: 3367 1278 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2334 2364 1178 Size: 30 165 30 931 30 4689 Fragments arranged by size: 4689 931 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2243 2672 1186 Size: 1057 429 4389 Fragments arranged by size: 4389 1057 429 BseYI C'CCAG_C Cuts at: 5078 5574 5078 Size: 496 5379 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2552 1163 Size: 573 816 4486 Fragments arranged by size: 4486 816 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3325 4086 5114 1540 Size: 275 186 1324 761 1028 2301 Fragments arranged by size: 2301 1324 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 2982 3936 4021 5092 2046 Size: 936 954 85 1071 2829 Fragments arranged by size: 2829 1071 954 936 85 BsmI GAATG_Cn' Cuts at: 3386 3479 3386 Size: 93 5782 BsmBI CGTCTCn'nnnn_ Cuts at: 2717 2717 Size: 5875 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2982 3194 3936 4021 5092 1391 Size: 399 256 936 212 742 85 1071 2174 Fragments arranged by size: 2174 1071 936 742 399 256 212 85 BspEI T'CCGG_A Cuts at: 5538 5538 Size: 5875 BspHI T'CATG_A Cuts at: 3764 5494 3764 Size: 1730 4145 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3230 844 Size: 2386 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3762 1394 Size: 429 1939 3507 Fragments arranged by size: 3507 1939 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4370 4544 65 Size: 1183 3122 174 1396 Fragments arranged by size: 3122 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2610 4519 4706 1700 Size: 110 800 1909 187 2869 Fragments arranged by size: 2869 1909 800 187 110 BsrGI T'GTAC_A Cuts at: 96 2466 96 Size: 2370 3505 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5875 BssSI C'ACGA_G Cuts at: 2279 3935 4947 2279 Size: 1656 1012 3207 Fragments arranged by size: 3207 1656 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2539 794 Size: 1745 4130 BstBI TT'CG_AA Cuts at: 4679 4679 Size: 5875 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5875 BstXI CCAn_nnnn'nTGG Cuts at: 2180 2180 Size: 5875 BstYI r'GATC_y Cuts at: 1972 2865 3205 3572 3957 3974 5426 5512 5524 Size: 893 340 367 385 17 1452 86 12 Cuts at: 5524 5870 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 893 385 367 346 340 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2481 2481 Size: 5875 BtgI C'CryG_G Cuts at: 513 1065 2160 513 Size: 552 1095 4228 Fragments arranged by size: 4228 1095 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5136 Fragments arranged by size: 5136 683 56 BtsI GCAGTG_nn' Cuts at: 3391 4185 4205 3391 Size: 794 20 5061 Fragments arranged by size: 5061 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5875 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5840 DraI TTT'AAA Cuts at: 3179 3179 Size: 5875 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4882 809 Size: 507 3566 1802 Fragments arranged by size: 3566 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2544 2847 3322 Size: 54 1425 50 275 18 714 303 475 Cuts at: 3322 4211 5840 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 714 475 303 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3322 1537 Size: 275 1510 4090 Fragments arranged by size: 4090 1510 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2297 3805 1353 Size: 944 1508 3423 Fragments arranged by size: 3423 1508 944 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4462 4836 4982 387 Size: 1064 138 2873 374 146 1280 Fragments arranged by size: 2873 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2980 2980 Size: 5875 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2181 2283 2321 2537 2633 2915 Size: 393 446 103 102 38 216 96 282 Cuts at: 2915 3023 3058 3938 4535 5322 5590 1239 Size: 108 35 880 597 787 268 1524 Fragments arranged by size: 1524 880 787 597 446 393 282 268 216 108 103 102 96 38 35 EcoNI CCTnn'n_nnAGG Cuts at: 2837 2837 Size: 5875 EcoRI G'AATT_C Cuts at: 2935 3184 3562 2935 Size: 249 378 5248 Fragments arranged by size: 5248 378 249 EcoRV GAT'ATC Cuts at: 3123 3123 Size: 5875 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2263 2263 Size: 5875 FspI TGC'GCA Cuts at: 4381 4381 Size: 5875 HaeII r_GCGC'y Cuts at: 1678 2710 5022 1678 Size: 1032 2312 2531 Fragments arranged by size: 2531 2312 1032 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2716 2748 4516 4548 4590 Size: 32 135 32 1144 32 1768 32 42 Cuts at: 4590 4622 1373 Size: 32 2626 Fragments arranged by size: 2626 1768 1144 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3219 3465 669 Size: 988 281 1281 246 3079 Fragments arranged by size: 3079 1281 988 281 246 HindIII A'AGCT_T Cuts at: 748 2372 2549 748 Size: 1624 177 4074 Fragments arranged by size: 4074 1624 177 HpaI GTT'AAC Cuts at: 3465 3465 Size: 5875 KpnI G_GTAC'C Cuts at: 2617 3200 2617 Size: 583 5292 MfeI C'AATT_G Cuts at: 3474 3474 Size: 5875 MluI A'CGCG_T Cuts at: 5751 5751 Size: 5875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2671 4989 5173 1305 Size: 1366 2318 184 2007 Fragments arranged by size: 2318 2007 1366 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2546 10 Size: 54 1425 343 714 3339 Fragments arranged by size: 3339 1425 714 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2240 2877 3161 3833 4192 4351 518 Size: 648 1074 637 284 672 359 159 2042 Fragments arranged by size: 2042 1074 672 648 637 359 284 159 MspA1I CmG'CkG Cuts at: 1606 2180 2299 2902 3291 3968 5116 5361 1606 Size: 574 119 603 389 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 603 574 389 245 119 NcoI C'CATG_G Cuts at: 513 1065 2160 513 Size: 552 1095 4228 Fragments arranged by size: 4228 1095 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5875 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5875 NotI GC'GGCC_GC Cuts at: 3322 3322 Size: 5875 NsiI A_TGCA'T Cuts at: 3027 3027 Size: 5875 NspI r_CATG'y Cuts at: 1132 1229 2887 3233 4778 1132 Size: 97 1658 346 1545 2229 Fragments arranged by size: 2229 1658 1545 346 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5875 PciI A'CATG_T Cuts at: 4774 4774 Size: 5875 PflMI CCAn_nnn'nTGG Cuts at: 1108 2875 1108 Size: 1767 4108 PfoI T'CCnGG_A Cuts at: 2322 2352 2973 2322 Size: 30 621 5224 Fragments arranged by size: 5224 621 30 PmeI GTTT'AAAC Cuts at: 3179 3179 Size: 5875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3944 3976 5457 5489 3944 Size: 32 1481 32 4330 Fragments arranged by size: 4330 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5875 PsiI TTA'TAA Cuts at: 3445 3445 Size: 5875 PstI C_TGCA'G Cuts at: 830 2703 3227 830 Size: 1873 524 3478 Fragments arranged by size: 3478 1873 524 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5875 PvuII CAG'CTG Cuts at: 2180 2299 2180 Size: 119 5756 SacI G_AGCT'C Cuts at: 2982 2982 Size: 5875 SalI G'TCGA_C Cuts at: 1936 3217 1936 Size: 1281 4594 SbfI CC_TGCA'GG Cuts at: 3227 3227 Size: 5875 ScaI AGT'ACT Cuts at: 4123 5835 4123 Size: 1712 4163 SfcI C'TryA_G Cuts at: 826 1045 2342 2699 3223 4358 5039 5230 826 Size: 219 1297 357 524 1135 681 191 1471 Fragments arranged by size: 1471 1297 1135 681 524 357 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5875 SmaI CCC'GGG Cuts at: 1872 3089 3108 3202 1872 Size: 1217 19 94 4545 Fragments arranged by size: 4545 1217 94 19 SmlI C'TyrA_G Cuts at: 820 1017 1943 2518 2888 2981 3979 4880 5142 Size: 197 926 575 370 93 998 901 262 Cuts at: 5142 820 Size: 1553 Fragments arranged by size: 1553 998 926 901 575 370 262 197 93 SnaBI TAC'GTA Cuts at: 493 3666 493 Size: 3173 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5875 SphI G_CATG'C Cuts at: 1132 3233 1132 Size: 2101 3774 SrfI GCCC'GGGC Cuts at: 3089 3089 Size: 5875 SspI AAT'ATT Cuts at: 5 52 2290 3799 5 Size: 47 2238 1509 2081 Fragments arranged by size: 2238 2081 1509 47 StuI AGG'CCT Cuts at: 1517 2738 1517 Size: 1221 4654 StyI C'CwwG_G Cuts at: 513 1065 2160 2386 3314 513 Size: 552 1095 226 928 3074 Fragments arranged by size: 3074 1095 928 552 226 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2061 3095 3902 3919 4072 4257 4702 Size: 1255 481 1034 807 17 153 185 445 Cuts at: 4702 325 Size: 1498 Fragments arranged by size: 1498 1255 1034 807 481 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2244 2316 2466 Size: 274 80 33 51 1443 267 72 150 Cuts at: 2466 4121 5833 96 Size: 1655 1712 138 Fragments arranged by size: 1712 1655 1443 274 267 150 138 80 72 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5835 5867 5835 Size: 32 5843 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5875 XbaI T'CTAG_A Cuts at: 3211 3211 Size: 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5875 XmaI C'CCGG_G Cuts at: 1870 3087 3106 3200 1870 Size: 1217 19 94 4545 Fragments arranged by size: 4545 1217 94 19 XmnI GAAnn'nnTTC Cuts at: 4004 4004 Size: 5875 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3683 276 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BbeI BbvCI Bpu10I BsiWI BstZ17I DraIII EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SwaI XcmI