Restriction map for
pFN21AE0116
(Linear) (Six-base) MAPSORT of: KIEE0116.seq Check: 6196 from: 1 to: 1170
KIEE0116.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
Acc65I G'GTAC_C
Cuts at: 0 608 1170
Size: 608 562
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 316 532 628 1053 1170
Size: 176 140 216 96 425 117
Fragments arranged by size:
425 216 176 140 117 96
AlwNI CAG_nnn'CTG
Cuts at: 0 332 1170
Size: 332 838
ApaLI G'TGCA_C
Cuts at: 0 37 1170
Size: 37 1133
ApoI r'AATT_y
Cuts at: 0 930 1008 1170
Size: 930 78 162
Fragments arranged by size:
930 162 78
AvaI C'yCGr_G
Cuts at: 0 1082 1101 1170
Size: 1082 19 69
Fragments arranged by size:
1082 69 19
AvrII C'CTAG_G
Cuts at: 0 381 1170
Size: 381 789
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 6 39 1170
Size: 6 33 1131
Fragments arranged by size:
1131 33 6
BanI G'GyrC_C
Cuts at: 0 608 1170
Size: 608 562
BanII G_rGCy'C
Cuts at: 0 977 1170
Size: 977 193
BbsI GAAGACnn'nnnn_
Cuts at: 0 151 526 1170
Size: 151 375 644
Fragments arranged by size:
644 375 151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1125 1159 1170
Size: 1125 34 11
Fragments arranged by size:
1125 34 11
BfrBI ATG'CAT
Cuts at: 0 1020 1170
Size: 1020 150
BglI GCCn_nnn'nGGC
Cuts at: 0 133 739 1170
Size: 133 606 431
Fragments arranged by size:
606 431 133
Bme1580I G_kGCm'C
Cuts at: 0 41 1170
Size: 41 1129
BmgBI CAC'GTC
Cuts at: 0 36 1170
Size: 36 1134
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 409 1170
Size: 409 761
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 278 910 1018 1170
Size: 73 205 632 108 152
Fragments arranged by size:
632 205 152 108 73
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 534 868 961 1170
Size: 534 334 93 209
Fragments arranged by size:
534 334 209 93
BsaHI Gr'CG_yC
Cuts at: 0 959 1170
Size: 959 211
BsaWI w'CCGG_w
Cuts at: 0 1025 1170
Size: 1025 145
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 329 359 1170
Size: 329 30 811
Fragments arranged by size:
811 329 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 238 667 1170
Size: 238 429 503
Fragments arranged by size:
503 429 238
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 1170
Size: 547 623
BsiHKAI G_wGCw'C
Cuts at: 0 41 977 1170
Size: 41 936 193
Fragments arranged by size:
936 193 41
BsmBI CGTCTCn'nnnn_
Cuts at: 0 712 1170
Size: 712 458
Bsp1286I G_dGCh'C
Cuts at: 0 41 977 1170
Size: 41 936 193
Fragments arranged by size:
936 193 41
BsrFI r'CCGG_y
Cuts at: 0 605 1170
Size: 605 565
BsrGI T'GTAC_A
Cuts at: 0 461 1170
Size: 461 709
BssSI C'ACGA_G
Cuts at: 0 274 1170
Size: 274 896
BstAPI GCAn_nnn'nTGC
Cuts at: 0 534 1170
Size: 534 636
BstXI CCAn_nnnn'nTGG
Cuts at: 0 175 1170
Size: 175 995
BstYI r'GATC_y
Cuts at: 0 860 1170
Size: 860 310
Bsu36I CC'TnA_GG
Cuts at: 0 476 1170
Size: 476 694
BtgI C'CryG_G
Cuts at: 0 155 1170
Size: 155 1015
EaeI y'GGCC_r
Cuts at: 0 539 842 1170
Size: 539 303 328
Fragments arranged by size:
539 328 303
EarI CTCTTCn'nnn_
Cuts at: 0 292 1170
Size: 292 878
EcoICRI GAG'CTC
Cuts at: 0 975 1170
Size: 975 195
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 176 278 316 532 628 910 1018
Size: 73 103 102 38 216 96 282 108
Cuts at: 1018 1053 1170
Size: 35 117
Fragments arranged by size:
282 216 117 108 103 102 96 73
38 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 832 1170
Size: 832 338
EcoRI G'AATT_C
Cuts at: 0 930 1170
Size: 930 240
EcoRV GAT'ATC
Cuts at: 0 1118 1170
Size: 1118 52
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 258 1170
Size: 258 912
HaeII r_GCGC'y
Cuts at: 0 705 1170
Size: 705 465
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 711 743 1170
Size: 711 32 427
Fragments arranged by size:
711 427 32
HindIII A'AGCT_T
Cuts at: 0 367 544 1170
Size: 367 177 626
Fragments arranged by size:
626 367 177
KpnI G_GTAC'C
Cuts at: 0 612 1170
Size: 612 558
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 666 1170
Size: 666 504
MscI TGG'CCA
Cuts at: 0 541 1170
Size: 541 629
MslI CAynn'nnrTG
Cuts at: 0 235 872 1156 1170
Size: 235 637 284 14
Fragments arranged by size:
637 284 235 14
MspA1I CmG'CkG
Cuts at: 0 175 294 897 1170
Size: 175 119 603 273
Fragments arranged by size:
603 273 175 119
NcoI C'CATG_G
Cuts at: 0 155 1170
Size: 155 1015
NsiI A_TGCA'T
Cuts at: 0 1022 1170
Size: 1022 148
NspI r_CATG'y
Cuts at: 0 882 1170
Size: 882 288
PflMI CCAn_nnn'nTGG
Cuts at: 0 870 1170
Size: 870 300
PfoI T'CCnGG_A
Cuts at: 0 317 347 968 1170
Size: 317 30 621 202
Fragments arranged by size:
621 317 202 30
PstI C_TGCA'G
Cuts at: 0 698 1170
Size: 698 472
PvuII CAG'CTG
Cuts at: 0 175 294 1170
Size: 175 119 876
Fragments arranged by size:
876 175 119
SacI G_AGCT'C
Cuts at: 0 977 1170
Size: 977 193
SfcI C'TryA_G
Cuts at: 0 337 694 1170
Size: 337 357 476
Fragments arranged by size:
476 357 337
SmaI CCC'GGG
Cuts at: 0 1084 1103 1170
Size: 1084 19 67
Fragments arranged by size:
1084 67 19
SmlI C'TyrA_G
Cuts at: 0 513 883 976 1170
Size: 513 370 93 194
Fragments arranged by size:
513 370 194 93
SrfI GCCC'GGGC
Cuts at: 0 1084 1170
Size: 1084 86
SspI AAT'ATT
Cuts at: 0 285 1170
Size: 285 885
StuI AGG'CCT
Cuts at: 0 733 1170
Size: 733 437
StyI C'CwwG_G
Cuts at: 0 155 381 1170
Size: 155 226 789
Fragments arranged by size:
789 226 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 56 1090 1170
Size: 56 1034 80
Fragments arranged by size:
1034 80 56
TatI w'GTAC_w
Cuts at: 0 239 311 461 1170
Size: 239 72 150 709
Fragments arranged by size:
709 239 150 72
XmaI C'CCGG_G
Cuts at: 0 1082 1101 1170
Size: 1082 19 69
Fragments arranged by size:
1082 69 19
Enzymes that do cut:
Acc65I AcuI AlwNI ApaLI ApoI AvaI AvrII BaeI
BanI BanII BbsI BcgI BfrBI BglI Bme1580I BmgBI
BplI BpmI BpuEI BsaHI BsaWI BsaXI BseRI BsgI
BsiHKAI BsmBI Bsp1286I BsrFI BsrGI BssSI BstAPI BstXI
BstYI Bsu36I BtgI EaeI EarI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI HaeII Hin4I HindIII KpnI MmeI
MscI MslI MspA1I NcoI NsiI NspI PflMI PfoI
PstI PvuII SacI SfcI SmaI SmlI SrfI SspI
StuI StyI TaqII TatI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
BamHI BbeI BbvCI BciVI BclI BglII BlpI BmrI
BmtI Bpu10I BsaI BsaAI BsaBI BseYI BsiEI BsiWI
BsmI BspEI BspHI BspMI BsrBI BsrDI BssHII BstBI
BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoO109I FseI FspI FspAI HincII
HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SwaI
TstI Tth111I XbaI XcmI XhoI XmnI ZraI