Restriction map for
pFN21AE0150
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BalI
BamHI BanII BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI Ksp632I KspI MamI
MfeI MflI MluI MluNI MroI
MscI MunI NciI NcoI NdeI
NheI NotI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TspRI Tth111I Van91I XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 916 GAATTC [GA]^AATT[CT]
AocI 481 CCTGAGG CC^T[ACGT]AGG
ApoI 916 GAATTC [GA]^AATT[CT]
AvaI 175 CCCGAG C^[CT]CG[GA]G
BbsI 740 GAAGAC GAAGAC(2/6)
BfaI 436 CTAG C^TAG
BpuAI 740 GAAGAC GAAGAC(2/6)
BseRI 484 GAGGAG GAGGAG(10/8)
BsgI 148 GTGCAG GTGCAG(16/14)
BsmAI 670 GTCTC GTCTC(1/5)
BsoBI 175 CCCGAG C^[CT]CG[GA]G
BspMI 144 GCAGGT ACCTGC(4/8)
BsrBI 124 GAGCGG CCGCTC(-3/-3)
BsrDI 681 CATTGC GCAATG(2/0)
BsrFI 76 GCCGGC [GA]^CCGG[CT]
BstXI 558 CCAAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 481 CCTGAGG CC^T[ACGT]AGG
Cfr10I 76 GCCGGC [GA]^CCGG[CT]
DpnII 168 GATC ^GATC
EaeI 74 TGGCCG [CT]^GGCC[GA]
Eco81I 481 CCTGAGG CC^T[ACGT]AGG
EcoRI 916 GAATTC G^AATTC
EcoT14I 558 CCAAGG C^C[AT][AT]GG
EcoT22I 1 ATGCAT ATGCA^T
FauI 126 GCGGG CCCGC(4/6)
HaeIII 75 GGCC GG^CC
HapII 77 CCGG C^CGG
HpaII 77 CCGG C^CGG
MaeI 436 CTAG C^TAG
MaeII 744 ACGT A^CGT
MboI 168 GATC ^GATC
MspI 77 CCGG C^CGG
NaeI 76 GCCGGC GCC^GGC
NdeII 168 GATC ^GATC
NgoMIV 76 GCCGGC G^CCGGC
NruI 222 TCGCGA TCG^CGA
NsiI 1 ATGCAT ATGCA^T
NspI 700 ACATGC [GA]CATG^[CT]
PleI 83 GAGTC GAGTC(4/5)
PstI 142 CTGCAG CTGCA^G
PvuII 92 CAGCTG CAG^CTG
Sau3AI 168 GATC ^GATC
SfcI 142 CTGCAG C^T[GA][CT]AG
SmlI 686 CTCAAG C^T[CT][GA]AG
StyI 558 CCAAGG C^C[AT][AT]GG
XbaI 435 TCTAGA T^CTAGA
XcmI 101 CCAAGACAGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AvaII 66 GGACC G^G[AT]CC
478 GGTCC
BanI 114 GGCGCC G^G[CT][GA]CC
847 GGCGCC
BbeI 114 GGCGCC GGCGC^C
847 GGCGCC
BpmI 338 CTGGAG CTGGAG(16/14)
363 CTGGAG
BsaJI 176 CCGAGG C^C[ACGT][ACGT]GG
558 CCAAGG
BsiYI 119 CCATCGAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
176 CCGAGGCGCGG
BslI 119 CCATCGAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
176 CCGAGGCGCGG
BssKI 207 CCTGG ^CC[ACGT]GG
384 CCTGG
BstF5I 53 CATCC GGATG(2/0)
413 CATCC
BstNI 207 CCTGG CC^[AT]GG
384 CCTGG
Cfr13I 66 GGACC G^G[ACGT]CC
478 GGTCC
DdeI 482 CTGAG C^T[ACGT]AG
792 CTTAG
DraII 65 AGGACCT [GA]G^G[ACGT]CC[CT]
477 AGGTCCT
EcoO109I 65 AGGACCT [GA]G^G[ACGT]CC[CT]
477 AGGTCCT
EcoRII 207 CCTGG ^CC[AT]GG
384 CCTGG
FokI 53 CATCC GGATG(9/13)
413 CATCC
HaeII 114 GGCGCC [GA]GCGC^[CT]
847 GGCGCC
HgaI 46 GACGC GACGC(5/10)
154 GCGTC
KasI 114 GGCGCC G^GCGCC
847 GGCGCC
MaeIII 509 GTGAC ^GT[ACGT]AC
694 GTGAC
MslI 413 CATCCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
585 CATCATTGTG
MspA1I 92 CAGCTG C[AC]G^C[GT]G
107 CAGCGG
MvaI 207 CCTGG CC^[AT]GG
384 CCTGG
NarI 114 GGCGCC GG^CGCC
847 GGCGCC
PpuMI 65 AGGACCT [GA]G^G[AT]CC[CT]
477 AGGTCCT
Sau96I 66 GGACC G^G[ACGT]CC
478 GGTCC
ScrFI 207 CCTGG CC^[ACGT]GG
384 CCTGG
SfoI 114 GGCGCC GGC^GCC
847 GGCGCC
TfiI 216 GATTC G^A[AT]TC
827 GAATC
Tsp45I 509 GTGAC ^GT[GC]AC
694 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Asp700I BbvI BsaHI BstUI Cac8I
CfoI Fnu4HI HhaI Hin1I HinP1I
HinfI HphI ItaI MboII MnlI
MseI MvnI MwoI NlaIII NlaIV
RsaI SfaNI TaqI Tru9I TseI
Tsp509I TthHB8I XmnI