Restriction map for
pFN21AE0150
(Circular) (Six-base) MAPSORT of: pFN21AE0150.seq Check: 9945 from: 1 to: 5650
pFN21AE0150 5650 bp
With 182 enzymes: SgfI *
October 20, 2009 10:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2140 2140
Size: 5650
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3460 278
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2993 1937
Size: 1056 4594
Acc65I G'GTAC_C
Cuts at: 2971 2971
Size: 5650
AclI AA'CG_TT
Cuts at: 1563 3777 4150 1563
Size: 2214 373 3063
Fragments arranged by size:
3063 2214 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2227 3713 5097 5365 1632
Size: 595 1486 1384 268 1917
Fragments arranged by size:
1917 1486 1384 595 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5453
AflIII A'CryG_T
Cuts at: 1203 4549 5526 1203
Size: 3346 977 1327
Fragments arranged by size:
3346 1327 977
AgeI A'CCGG_T
Cuts at: 4481 4481
Size: 5650
AhdI GACnn_n'nnGTC
Cuts at: 4379 4379
Size: 5650
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5650
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5650
AlwNI CAG_nnn'CTG
Cuts at: 3393 4965 3393
Size: 1572 4078
ApaLI G'TGCA_C
Cuts at: 3707 4863 3707
Size: 1156 4494
ApoI r'AATT_y
Cuts at: 1493 2921 2959 3185 3337 5446 1493
Size: 1428 38 226 152 2109 1697
Fragments arranged by size:
2109 1697 1428 226 152 38
AseI AT'TA_AT
Cuts at: 160 1032 4204 160
Size: 872 3172 1606
Fragments arranged by size:
3172 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
AvaI C'yCGr_G
Cuts at: 1870 1943 2180 2975 1870
Size: 73 237 795 4545
Fragments arranged by size:
4545 795 237 73
BamHI G'GATC_C
Cuts at: 2980 3347 2980
Size: 367 5283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2119 2852 2971 4426 618
Size: 325 217 627 332 733 119 1455 1842
Fragments arranged by size:
1842 1455 733 627 332 325 217 119
BanII G_rGCy'C
Cuts at: 1391 2969 1391
Size: 1578 4072
BbeI G_GCGC'C
Cuts at: 2123 2856 2123
Size: 733 4917
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2752 928
Size: 971 853 3826
Fragments arranged by size:
3826 971 853
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3841 3875 1903
Size: 34 50 34 1820 34 3678
Fragments arranged by size:
3678 1820 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3542 4752 3542
Size: 1210 4440
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5650
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 5650
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4261 136
Size: 107 122 71 631 605 2589 1525
Fragments arranged by size:
2589 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5645 5645
Size: 5650
BlpI GC'TnA_GC
Cuts at: 3067 3067
Size: 5650
Bme1580I G_kGCm'C
Cuts at: 1790 3711 4867 1790
Size: 1921 1156 2573
Fragments arranged by size:
2573 1921 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5650
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3381 4339 442
Size: 416 53 83 399 24 1964 958 1753
Fragments arranged by size:
1964 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5650
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2364 2389 4310 1239
Size: 1125 25 1921 2579
Fragments arranged by size:
2579 1921 1125 25
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2676 3775 4640 4938 2676
Size: 1099 865 298 3388
Fragments arranged by size:
3388 1099 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4313 882
Size: 617 2814 2219
Fragments arranged by size:
2814 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3441 493
Size: 711 2237 2702
Fragments arranged by size:
2702 2237 711
BsaBI GATnn'nnATC
Cuts at: 5644 5644
Size: 5650
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2052 2120 2159 2853
Size: 53 83 186 936 519 68 39 694
Cuts at: 2853 3457 3839 4474 275
Size: 604 382 635 1451
Fragments arranged by size:
1451 936 694 635 604 519 382 186
83 68 53 39
BsaWI w'CCGG_w
Cuts at: 4083 4481 4755 4902 5313 4083
Size: 398 274 147 411 4420
Fragments arranged by size:
4420 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5425
Fragments arranged by size:
5425 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2504 1186
Size: 1318 4332
BseYI C'CCAG_C
Cuts at: 2408 4853 5349 2408
Size: 2445 496 2709
Fragments arranged by size:
2709 2445 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2174 1163
Size: 573 438 4639
Fragments arranged by size:
4639 573 438
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3100 3861 4889 1540
Size: 275 186 1099 761 1028 2301
Fragments arranged by size:
2301 1099 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3711 3796 4867 3711
Size: 85 1071 4494
Fragments arranged by size:
4494 1071 85
BsmI GAATG_Cn'
Cuts at: 3161 3254 3161
Size: 93 5557
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2969 3711 3796 4867 1391
Size: 399 1179 742 85 1071 2174
Fragments arranged by size:
2174 1179 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5313 5313
Size: 5650
BspHI T'CATG_A
Cuts at: 3539 5269 3539
Size: 1730 3920
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2140 3005 844
Size: 1296 865 3489
Fragments arranged by size:
3489 1296 865
BsrBI CCG'CTC
Cuts at: 1394 1823 2131 3537 1394
Size: 429 308 1406 3507
Fragments arranged by size:
3507 1406 429 308
BsrDI GCAATG_nn'
Cuts at: 65 1248 2685 4145 4319 65
Size: 1183 1437 1460 174 1396
Fragments arranged by size:
1460 1437 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2081 4294 4481 1700
Size: 110 271 2213 187 2869
Fragments arranged by size:
2869 2213 271 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5650
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5650
BssSI C'ACGA_G
Cuts at: 3710 4722 3710
Size: 1012 4638
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5650
BstBI TT'CG_AA
Cuts at: 4454 4454
Size: 5650
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5650
BstXI CCAn_nnnn'nTGG
Cuts at: 2570 2570
Size: 5650
BstYI r'GATC_y
Cuts at: 1972 2980 3347 3732 3749 5201 5287 5299 5645
Size: 1008 367 385 17 1452 86 12 346
Cuts at: 5645 1972
Size: 1977
Fragments arranged by size:
1977 1452 1008 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2487 2487
Size: 5650
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5098
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4911
Fragments arranged by size:
4911 683 56
BtsI GCAGTG_nn'
Cuts at: 3166 3960 3980 3166
Size: 794 20 4836
Fragments arranged by size:
4836 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5650
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5615
DraI TTT'AAA
Cuts at: 2954 2954
Size: 5650
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4657 809
Size: 507 3341 1802
Fragments arranged by size:
3341 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 3097 3986
Size: 54 1425 50 275 18 249 1018 889
Cuts at: 3986 5615 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1018 889 275 249 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3097 1537
Size: 275 1285 4090
Fragments arranged by size:
4090 1285 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3580 1353
Size: 2227 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2100 4237 4611 4757 387
Size: 1064 138 511 2137 374 146 1280
Fragments arranged by size:
2137 1280 1064 511 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2227 2364 2389 3713 4310 5097 5365
Size: 393 595 137 25 1324 597 787 268
Cuts at: 5365 1239
Size: 1524
Fragments arranged by size:
1524 1324 787 597 595 393 268 137
25
EcoO109I rG'GnC_Cy
Cuts at: 2071 2483 2071
Size: 412 5238
EcoRI G'AATT_C
Cuts at: 2921 2959 3337 2921
Size: 38 378 5234
Fragments arranged by size:
5234 378 38
FspI TGC'GCA
Cuts at: 4156 4156
Size: 5650
HaeII r_GCGC'y
Cuts at: 1678 2123 2856 4797 1678
Size: 445 733 1941 2531
Fragments arranged by size:
2531 1941 733 445
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2042 2074 2451 2483 4291
Size: 32 135 32 470 32 377 32 1808
Cuts at: 4291 4323 4365 4397 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1808 470 377 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2994 3240 669
Size: 988 281 1056 246 3079
Fragments arranged by size:
3079 1056 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5650
HpaI GTT'AAC
Cuts at: 3240 3240
Size: 5650
KasI G'GCGC_C
Cuts at: 2119 2852 2119
Size: 733 4917
KpnI G_GTAC'C
Cuts at: 2975 2975
Size: 5650
MfeI C'AATT_G
Cuts at: 3249 3249
Size: 5650
MluI A'CGCG_T
Cuts at: 5526 5526
Size: 5650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2312 4764 4948 1305
Size: 1007 2452 184 2007
Fragments arranged by size:
2452 2007 1007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3828
Fragments arranged by size:
3828 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2422 2594 3608 3967 4126 518
Size: 648 1256 172 1014 359 159 2042
Fragments arranged by size:
2042 1256 1014 648 359 172 159
MspA1I CmG'CkG
Cuts at: 1606 2099 2114 3066 3743 4891 5136 1606
Size: 493 15 952 677 1148 245 2120
Fragments arranged by size:
2120 1148 952 677 493 245 15
NaeI GCC'GGC
Cuts at: 2083 2083
Size: 5650
NarI GG'CG_CC
Cuts at: 2120 2853 2120
Size: 733 4917
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5098
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5650
NgoMIV G'CCGG_C
Cuts at: 2081 2081
Size: 5650
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5650
NotI GC'GGCC_GC
Cuts at: 3097 3097
Size: 5650
NruI TCG'CGA
Cuts at: 2229 2229
Size: 5650
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 5650
NspI r_CATG'y
Cuts at: 1132 1229 2709 3008 4553 1132
Size: 97 1480 299 1545 2229
Fragments arranged by size:
2229 1545 1480 299 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5650
PciI A'CATG_T
Cuts at: 4549 4549
Size: 5650
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5650
PmeI GTTT'AAAC
Cuts at: 2954 2954
Size: 5650
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3719 3751 5232 5264 3719
Size: 32 1481 32 4105
Fragments arranged by size:
4105 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2071 2483 2071
Size: 412 5238
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5650
PsiI TTA'TAA
Cuts at: 3220 3220
Size: 5650
PstI C_TGCA'G
Cuts at: 830 2151 3002 830
Size: 1321 851 3478
Fragments arranged by size:
3478 1321 851
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5650
PvuII CAG'CTG
Cuts at: 2099 2099
Size: 5650
SalI G'TCGA_C
Cuts at: 1936 2992 1936
Size: 1056 4594
SbfI CC_TGCA'GG
Cuts at: 3002 3002
Size: 5650
ScaI AGT'ACT
Cuts at: 3898 5610 3898
Size: 1712 3938
SfcI C'TryA_G
Cuts at: 826 1045 2147 2998 4133 4814 5005 826
Size: 219 1102 851 1135 681 191 1471
Fragments arranged by size:
1471 1135 1102 851 681 219 191
SfoI GGC'GCC
Cuts at: 2121 2854 2121
Size: 733 4917
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5650
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5650
SmaI CCC'GGG
Cuts at: 1872 2977 1872
Size: 1105 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2691 3754 4655 4917 820
Size: 197 926 748 1063 901 262 1553
Fragments arranged by size:
1553 1063 926 901 748 262 197
SnaBI TAC'GTA
Cuts at: 493 3441 493
Size: 2948 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5650
SphI G_CATG'C
Cuts at: 1132 3008 1132
Size: 1876 3774
SspI AAT'ATT
Cuts at: 5 52 3574 5
Size: 47 3522 2081
Fragments arranged by size:
3522 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5650
StyI C'CwwG_G
Cuts at: 513 1065 2563 3089 513
Size: 552 1498 526 3074
Fragments arranged by size:
3074 1498 552 526
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3677 3694 3847 4032 4477 325
Size: 1255 2097 17 153 185 445 1498
Fragments arranged by size:
2097 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2581 3896 5608
Size: 274 80 33 51 1443 604 1315 1712
Cuts at: 5608 96
Size: 138
Fragments arranged by size:
1712 1443 1315 604 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5610 5642 5610
Size: 32 5618
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5650
XbaI T'CTAG_A
Cuts at: 2440 2986 2440
Size: 546 5104
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2113 2113
Size: 5650
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5650
XmaI C'CCGG_G
Cuts at: 1870 2975 1870
Size: 1105 4545
XmnI GAAnn'nnTTC
Cuts at: 2719 2836 2925 3779 2719
Size: 117 89 854 4590
Fragments arranged by size:
4590 854 117 89
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3458 276
Size: 53 83 186 936 1924 2468
Fragments arranged by size:
2468 1924 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FalI FseI FspAI PacI PfoI PmlI PspOMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI