(Circular) (Six-base) MAPSORT of: pFN21AE0005.seq Check: 6642 from: 1 to: 6808 pFN21AE0005 6808 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4618 278 Size: 53 83 186 936 3082 2468 Fragments arranged by size: 3082 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4151 1937 Size: 2214 4594 Acc65I G'GTAC_C Cuts at: 3843 4129 3843 Size: 286 6522 AclI AA'CG_TT Cuts at: 1563 4935 5308 1563 Size: 3372 373 3063 Fragments arranged by size: 3372 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2658 3374 3765 4103 4871 6255 6523 1632 Size: 1026 716 391 338 768 1384 268 1917 Fragments arranged by size: 1917 1384 1026 768 716 391 338 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6611 AflIII A'CryG_T Cuts at: 1203 3878 5707 6684 1203 Size: 2675 1829 977 1327 Fragments arranged by size: 2675 1829 1327 977 AgeI A'CCGG_T Cuts at: 5639 5639 Size: 6808 AhdI GACnn_n'nnGTC Cuts at: 5537 5537 Size: 6808 AleI CACnn'nnGTG Cuts at: 1166 2862 1166 Size: 1696 5112 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6808 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3306 3338 3306 Size: 32 6776 AlwNI CAG_nnn'CTG Cuts at: 4551 6123 4551 Size: 1572 5236 ApaLI G'TGCA_C Cuts at: 4865 6021 4865 Size: 1156 5652 ApoI r'AATT_y Cuts at: 1493 2483 2549 2686 2693 3196 3482 3615 3712 Size: 990 66 137 7 503 286 133 97 Cuts at: 3712 3729 4117 4343 4495 6604 1493 Size: 17 388 226 152 2109 1697 Fragments arranged by size: 2109 1697 990 503 388 286 226 152 137 133 97 66 17 7 AseI AT'TA_AT Cuts at: 160 1032 5362 160 Size: 872 4330 1606 Fragments arranged by size: 4330 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6808 AvaI C'yCGr_G Cuts at: 1870 1943 4133 1870 Size: 73 2190 4545 Fragments arranged by size: 4545 2190 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2322 2355 2322 Size: 33 6775 BamHI G'GATC_C Cuts at: 4138 4505 4138 Size: 367 6441 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3843 4129 5584 618 Size: 325 217 627 2056 286 1455 1842 Fragments arranged by size: 2056 1842 1455 627 325 286 217 BanII G_rGCy'C Cuts at: 1391 4127 1391 Size: 2736 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3405 3702 928 Size: 971 1506 297 4034 Fragments arranged by size: 4034 1506 971 297 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4999 5033 1903 Size: 34 3062 34 3678 Fragments arranged by size: 3678 3062 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3560 3797 4700 5910 3560 Size: 237 903 1210 4458 Fragments arranged by size: 4458 1210 903 237 BclI T'GATC_A Cuts at: 1548 2059 1548 Size: 511 6297 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5419 136 Size: 107 122 71 631 605 3747 1525 Fragments arranged by size: 3747 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2224 6803 2224 Size: 4579 2229 BlpI GC'TnA_GC Cuts at: 4225 4225 Size: 6808 Bme1580I G_kGCm'C Cuts at: 1790 4869 6025 1790 Size: 3079 1156 2573 Fragments arranged by size: 3079 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6808 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4539 5497 442 Size: 416 53 83 399 24 3122 958 1753 Fragments arranged by size: 3122 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6808 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3051 5468 1239 Size: 1812 2417 2579 Fragments arranged by size: 2579 2417 1812 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2210 4933 5798 6096 2210 Size: 2723 865 298 2922 Fragments arranged by size: 2922 2723 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5471 882 Size: 617 3972 2219 Fragments arranged by size: 3972 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4599 493 Size: 711 3395 2702 Fragments arranged by size: 3395 2702 711 BsaBI GATnn'nnATC Cuts at: 6802 6802 Size: 6808 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4615 4997 5632 275 Size: 53 83 186 936 3082 382 635 1451 Fragments arranged by size: 3082 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5241 5639 5913 6060 6471 5241 Size: 398 274 147 411 5578 Fragments arranged by size: 5578 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3648 3678 1178 Size: 30 165 30 2245 30 4308 Fragments arranged by size: 4308 2245 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3764 1186 Size: 2578 4230 BseYI C'CCAG_C Cuts at: 6011 6507 6011 Size: 496 6312 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3689 1163 Size: 573 1953 4282 Fragments arranged by size: 4282 1953 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4258 5019 6047 1540 Size: 275 186 2257 761 1028 2301 Fragments arranged by size: 2301 2257 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4869 4954 6025 4869 Size: 85 1071 5652 Fragments arranged by size: 5652 1071 85 BsmI GAATG_Cn' Cuts at: 2891 4319 4412 2891 Size: 1428 93 5287 Fragments arranged by size: 5287 1428 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4127 4869 4954 6025 1391 Size: 399 2337 742 85 1071 2174 Fragments arranged by size: 2337 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6471 6471 Size: 6808 BspHI T'CATG_A Cuts at: 2404 3979 4697 6427 2404 Size: 1575 718 1730 2785 Fragments arranged by size: 2785 1730 1575 718 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4163 844 Size: 3319 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4695 1394 Size: 429 2872 3507 Fragments arranged by size: 3507 2872 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3051 3515 5303 5477 65 Size: 1183 1803 464 1788 174 1396 Fragments arranged by size: 1803 1788 1396 1183 464 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5452 5639 1700 Size: 110 3642 187 2869 Fragments arranged by size: 3642 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6808 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6808 BssSI C'ACGA_G Cuts at: 4868 5880 4868 Size: 1012 5796 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6808 BstBI TT'CG_AA Cuts at: 5612 5612 Size: 6808 BstEII G'GTnAC_C Cuts at: 1184 2294 1184 Size: 1110 5698 BstYI r'GATC_y Cuts at: 1972 2224 4138 4505 4890 4907 6359 6445 6457 Size: 252 1914 367 385 17 1452 86 12 Cuts at: 6457 6803 1972 Size: 346 1977 Fragments arranged by size: 1977 1914 1452 385 367 346 252 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3230 3230 Size: 6808 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5317 Fragments arranged by size: 5317 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6069 Fragments arranged by size: 6069 683 56 BtsI GCAGTG_nn' Cuts at: 2812 3845 4324 5118 5138 2812 Size: 1033 479 794 20 4482 Fragments arranged by size: 4482 1033 794 479 20 ClaI AT'CG_AT Cuts at: 1554 2154 1554 Size: 600 6208 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6773 DraI TTT'AAA Cuts at: 2662 2704 3201 3889 4112 2662 Size: 42 497 688 223 5358 Fragments arranged by size: 5358 688 497 223 42 DraIII CAC_nnn'GTG Cuts at: 2099 2099 Size: 6808 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3989 5815 809 Size: 507 2673 1826 1802 Fragments arranged by size: 2673 1826 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4255 5144 6773 Size: 54 1425 50 275 18 2425 889 1629 Cuts at: 6773 8 Size: 43 Fragments arranged by size: 2425 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4255 1537 Size: 275 2443 4090 Fragments arranged by size: 4090 2443 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2634 3741 4738 1353 Size: 1281 1107 997 3423 Fragments arranged by size: 3423 1281 1107 997 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5395 5769 5915 387 Size: 1064 138 3806 374 146 1280 Fragments arranged by size: 3806 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2658 3051 3374 3765 4103 4871 5468 Size: 393 1026 393 323 391 338 768 597 Cuts at: 5468 6255 6523 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1026 787 768 597 393 393 391 338 323 268 EcoNI CCTnn'n_nnAGG Cuts at: 2927 2927 Size: 6808 EcoRI G'AATT_C Cuts at: 2483 3482 4117 4495 2483 Size: 999 635 378 4796 Fragments arranged by size: 4796 999 635 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3482 3482 Size: 6808 FspI TGC'GCA Cuts at: 3584 5314 3584 Size: 1730 5078 FspAI rTGC'GCAy Cuts at: 3584 3584 Size: 6808 HaeII r_GCGC'y Cuts at: 1678 5955 1678 Size: 4277 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3142 3174 3617 3649 3740 Size: 32 135 32 1570 32 443 32 91 Cuts at: 3740 3772 5449 5481 5523 5555 1373 Size: 32 1677 32 42 32 2626 Fragments arranged by size: 2626 1677 1570 443 135 91 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2752 4152 4398 669 Size: 988 281 814 1400 246 3079 Fragments arranged by size: 3079 1400 988 814 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6808 HpaI GTT'AAC Cuts at: 4398 4398 Size: 6808 KpnI G_GTAC'C Cuts at: 3847 4133 3847 Size: 286 6522 MfeI C'AATT_G Cuts at: 4407 4407 Size: 6808 MluI A'CGCG_T Cuts at: 6684 6684 Size: 6808 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2047 3489 5922 6106 1305 Size: 742 1442 2433 184 2007 Fragments arranged by size: 2433 2007 1442 742 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4986 Fragments arranged by size: 4986 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2862 4014 4766 5125 5284 518 Size: 648 1696 1152 752 359 159 2042 Fragments arranged by size: 2042 1696 1152 752 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4224 4901 6049 6294 1606 Size: 2618 677 1148 245 2120 Fragments arranged by size: 2618 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5317 Fragments arranged by size: 5317 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6808 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6808 NotI GC'GGCC_GC Cuts at: 4255 4255 Size: 6808 NspI r_CATG'y Cuts at: 1132 1229 3882 4166 5711 1132 Size: 97 2653 284 1545 2229 Fragments arranged by size: 2653 2229 1545 284 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6808 PciI A'CATG_T Cuts at: 3878 5707 3878 Size: 1829 4979 PflMI CCAn_nnn'nTGG Cuts at: 1108 3800 3970 1108 Size: 2692 170 3946 Fragments arranged by size: 3946 2692 170 PmeI GTTT'AAAC Cuts at: 4112 4112 Size: 6808 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4877 4909 6390 6422 4877 Size: 32 1481 32 5263 Fragments arranged by size: 5263 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6808 PsiI TTA'TAA Cuts at: 4378 4378 Size: 6808 PstI C_TGCA'G Cuts at: 830 4160 830 Size: 3330 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6808 SalI G'TCGA_C Cuts at: 1936 4150 1936 Size: 2214 4594 SbfI CC_TGCA'GG Cuts at: 4160 4160 Size: 6808 ScaI AGT'ACT Cuts at: 3798 5056 6768 3798 Size: 1258 1712 3838 Fragments arranged by size: 3838 1712 1258 SexAI A'CCwGG_T Cuts at: 3735 3916 3735 Size: 181 6627 SfcI C'TryA_G Cuts at: 826 1045 2568 3135 3872 3925 4156 5291 5972 Size: 219 1523 567 737 53 231 1135 681 Cuts at: 5972 6163 826 Size: 191 1471 Fragments arranged by size: 1523 1471 1135 737 681 567 231 219 191 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6808 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6808 SmaI CCC'GGG Cuts at: 1872 4135 1872 Size: 2263 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2189 4912 5813 6075 820 Size: 197 926 246 2723 901 262 1553 Fragments arranged by size: 2723 1553 926 901 262 246 197 SnaBI TAC'GTA Cuts at: 493 4599 493 Size: 4106 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6808 SphI G_CATG'C Cuts at: 1132 4166 1132 Size: 3034 3774 SspI AAT'ATT Cuts at: 5 52 2666 3085 3127 3811 4732 5 Size: 47 2614 419 42 684 921 2081 Fragments arranged by size: 2614 2081 921 684 419 47 42 StuI AGG'CCT Cuts at: 1517 2875 3012 1517 Size: 1358 137 5313 Fragments arranged by size: 5313 1358 137 StyI C'CwwG_G Cuts at: 513 1065 2004 4247 513 Size: 552 939 2243 3074 Fragments arranged by size: 3074 2243 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3120 3551 4835 4852 5005 5190 5635 Size: 1255 1540 431 1284 17 153 185 445 Cuts at: 5635 325 Size: 1498 Fragments arranged by size: 1540 1498 1284 1255 445 431 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2283 3796 5054 Size: 274 80 33 51 1443 306 1513 1258 Cuts at: 5054 6766 96 Size: 1712 138 Fragments arranged by size: 1712 1513 1443 1258 306 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2757 2789 6768 6800 2757 Size: 32 3979 32 2765 Fragments arranged by size: 3979 2765 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3156 1316 Size: 1840 4968 XbaI T'CTAG_A Cuts at: 4144 4144 Size: 6808 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6808 XmaI C'CCGG_G Cuts at: 1870 4133 1870 Size: 2263 4545 XmnI GAAnn'nnTTC Cuts at: 4937 4937 Size: 6808 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4616 276 Size: 53 83 186 936 3082 2468 Fragments arranged by size: 3082 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoO109I EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI