(Circular) (Six-base) MAPSORT of: pFN21AE0408.seq Check: 4540 from: 1 to: 5692 pFN21AE0408 5692 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2215 2215 Size: 5692 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3502 278 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2652 3035 1937 Size: 715 383 4594 Fragments arranged by size: 4594 715 383 Acc65I G'GTAC_C Cuts at: 2828 3013 2828 Size: 185 5507 AclI AA'CG_TT Cuts at: 1563 3819 4192 1563 Size: 2256 373 3063 Fragments arranged by size: 3063 2256 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2055 2118 2184 3755 5139 5407 1632 Size: 423 63 66 1571 1384 268 1917 Fragments arranged by size: 1917 1571 1384 423 268 66 63 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5495 AflIII A'CryG_T Cuts at: 1203 4591 5568 1203 Size: 3388 977 1327 Fragments arranged by size: 3388 1327 977 AgeI A'CCGG_T Cuts at: 4523 4523 Size: 5692 AhdI GACnn_n'nnGTC Cuts at: 4421 4421 Size: 5692 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5692 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5692 AlwNI CAG_nnn'CTG Cuts at: 2243 3435 5007 2243 Size: 1192 1572 2928 Fragments arranged by size: 2928 1572 1192 ApaLI G'TGCA_C Cuts at: 3749 4905 3749 Size: 1156 4536 ApoI r'AATT_y Cuts at: 1493 2253 3001 3227 3379 5488 1493 Size: 760 748 226 152 2109 1697 Fragments arranged by size: 2109 1697 760 748 226 152 AseI AT'TA_AT Cuts at: 160 1032 4246 160 Size: 872 3214 1606 Fragments arranged by size: 3214 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 AvaI C'yCGr_G Cuts at: 1870 1943 3017 1870 Size: 73 1074 4545 Fragments arranged by size: 4545 1074 73 BamHI G'GATC_C Cuts at: 3022 3389 3022 Size: 367 5325 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2227 2828 3013 4468 618 Size: 325 217 627 440 601 185 1455 1842 Fragments arranged by size: 1842 1455 627 601 440 325 217 185 BanII G_rGCy'C Cuts at: 1391 2022 2502 2697 3011 1391 Size: 631 480 195 314 4072 Fragments arranged by size: 4072 631 480 314 195 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4721 BbvCI CC'TCA_GC Cuts at: 2106 2106 Size: 5692 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3883 3917 1903 Size: 34 1946 34 3678 Fragments arranged by size: 3678 1946 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2812 3584 4794 2812 Size: 772 1210 3710 Fragments arranged by size: 3710 1210 772 BclI T'GATC_A Cuts at: 1548 1548 Size: 5692 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4303 136 Size: 107 122 71 631 605 2631 1525 Fragments arranged by size: 2631 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5687 5687 Size: 5692 BlpI GC'TnA_GC Cuts at: 3109 3109 Size: 5692 Bme1580I G_kGCm'C Cuts at: 1790 2128 2232 2720 3753 4909 1790 Size: 338 104 488 1033 1156 2573 Fragments arranged by size: 2573 1156 1033 488 338 104 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5692 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2671 3423 4381 Size: 416 53 83 399 24 1254 752 958 Cuts at: 4381 442 Size: 1753 Fragments arranged by size: 1753 1254 958 752 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5692 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2077 2548 2077 Size: 471 5221 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2354 2543 2891 2972 4352 1239 Size: 1115 189 348 81 1380 2579 Fragments arranged by size: 2579 1380 1115 348 189 81 Bpu10I CC'TnA_GC Cuts at: 2106 2106 Size: 5692 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3817 4682 4980 3817 Size: 865 298 4529 Fragments arranged by size: 4529 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4355 882 Size: 617 2856 2219 Fragments arranged by size: 2856 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3483 493 Size: 711 2279 2702 Fragments arranged by size: 2702 2279 711 BsaBI GATnn'nnATC Cuts at: 5686 5686 Size: 5692 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3499 3881 4516 275 Size: 53 83 186 936 1966 382 635 1451 Fragments arranged by size: 1966 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2593 4125 4523 4797 4944 5355 2593 Size: 1532 398 274 147 411 2930 Fragments arranged by size: 2930 1532 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5467 Fragments arranged by size: 5467 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2057 2060 2069 2777 2786 2789 2969 Size: 868 3 3 9 708 9 3 180 Cuts at: 2969 2972 1186 Size: 3 3906 Fragments arranged by size: 3906 868 708 180 9 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 2021 2858 4895 5391 2021 Size: 837 2037 496 2322 Fragments arranged by size: 2322 2037 837 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2630 1163 Size: 573 894 4225 Fragments arranged by size: 4225 894 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3142 3903 4931 1540 Size: 275 186 1141 761 1028 2301 Fragments arranged by size: 2301 1141 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2061 2502 3753 3838 4909 2061 Size: 441 1251 85 1071 2844 Fragments arranged by size: 2844 1251 1071 441 85 BsmI GAATG_Cn' Cuts at: 3203 3296 3203 Size: 93 5599 BsmBI CGTCTCn'nnnn_ Cuts at: 2519 2519 Size: 5692 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2061 2128 2232 2502 2697 2720 Size: 399 232 39 67 104 270 195 23 Cuts at: 2720 3011 3753 3838 4909 1391 Size: 291 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 291 270 232 195 104 85 67 39 23 BspEI T'CCGG_A Cuts at: 5355 5355 Size: 5692 BspHI T'CATG_A Cuts at: 3581 5311 3581 Size: 1730 3962 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2215 3047 844 Size: 1371 832 3489 Fragments arranged by size: 3489 1371 832 BsrBI CCG'CTC Cuts at: 1394 1823 3579 1394 Size: 429 1756 3507 Fragments arranged by size: 3507 1756 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4187 4361 65 Size: 1183 2939 174 1396 Fragments arranged by size: 2939 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2207 4336 4523 1700 Size: 110 397 2129 187 2869 Fragments arranged by size: 2869 2129 397 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5692 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5692 BssSI C'ACGA_G Cuts at: 2429 3752 4764 2429 Size: 1323 1012 3357 Fragments arranged by size: 3357 1323 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5692 BstBI TT'CG_AA Cuts at: 4496 4496 Size: 5692 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5692 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5692 BstYI r'GATC_y Cuts at: 1972 3022 3389 3774 3791 5243 5329 5341 5687 Size: 1050 367 385 17 1452 86 12 346 Cuts at: 5687 1972 Size: 1977 Fragments arranged by size: 1977 1452 1050 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2244 2244 Size: 5692 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4201 Fragments arranged by size: 4201 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4953 Fragments arranged by size: 4953 683 56 BtsI GCAGTG_nn' Cuts at: 2321 3208 4002 4022 2321 Size: 887 794 20 3991 Fragments arranged by size: 3991 887 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5692 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5657 DraI TTT'AAA Cuts at: 2996 2996 Size: 5692 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4699 809 Size: 507 3383 1802 Fragments arranged by size: 3383 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3139 4028 5657 Size: 54 1425 50 275 18 1309 889 1629 Cuts at: 5657 8 Size: 43 Fragments arranged by size: 1629 1425 1309 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3139 1537 Size: 275 1327 4090 Fragments arranged by size: 4090 1327 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2031 2046 3622 1353 Size: 678 15 1576 3423 Fragments arranged by size: 3423 1576 678 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4279 4653 4799 387 Size: 1064 138 2690 374 146 1280 Fragments arranged by size: 2690 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2500 2500 Size: 5692 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2055 2118 2184 2354 2543 2891 2972 Size: 393 423 63 66 170 189 348 81 Cuts at: 2972 3755 4352 5139 5407 1239 Size: 783 597 787 268 1524 Fragments arranged by size: 1524 787 783 597 423 393 348 268 189 170 81 66 63 EcoNI CCTnn'n_nnAGG Cuts at: 2309 2951 2309 Size: 642 5050 EcoRI G'AATT_C Cuts at: 3001 3379 3001 Size: 378 5314 FspI TGC'GCA Cuts at: 4198 4198 Size: 5692 HaeII r_GCGC'y Cuts at: 1678 4839 1678 Size: 3161 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2303 2335 4333 4365 4407 Size: 32 135 32 731 32 1998 32 42 Cuts at: 4407 4439 1373 Size: 32 2626 Fragments arranged by size: 2626 1998 731 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2272 3036 3282 669 Size: 988 281 334 764 246 3079 Fragments arranged by size: 3079 988 764 334 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5692 HpaI GTT'AAC Cuts at: 3282 3282 Size: 5692 KpnI G_GTAC'C Cuts at: 2832 3017 2832 Size: 185 5507 MfeI C'AATT_G Cuts at: 3291 3291 Size: 5692 MluI A'CGCG_T Cuts at: 5568 5568 Size: 5692 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2511 2765 4806 4990 1305 Size: 1206 254 2041 184 2007 Fragments arranged by size: 2041 2007 1206 254 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3870 Fragments arranged by size: 3870 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3650 4009 4168 518 Size: 648 843 1641 359 159 2042 Fragments arranged by size: 2042 1641 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2917 3108 3785 4933 5178 1606 Size: 1311 191 677 1148 245 2120 Fragments arranged by size: 2120 1311 1148 677 245 191 NaeI GCC'GGC Cuts at: 2209 2209 Size: 5692 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4201 Fragments arranged by size: 4201 939 552 NdeI CA'TA_TG Cuts at: 387 2629 387 Size: 2242 3450 NgoMIV G'CCGG_C Cuts at: 2207 2207 Size: 5692 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5692 NotI GC'GGCC_GC Cuts at: 3139 3139 Size: 5692 NspI r_CATG'y Cuts at: 1132 1229 3050 4595 1132 Size: 97 1821 1545 2229 Fragments arranged by size: 2229 1821 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5692 PciI A'CATG_T Cuts at: 4591 4591 Size: 5692 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5692 PmeI GTTT'AAAC Cuts at: 2996 2996 Size: 5692 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3761 3793 5274 5306 3761 Size: 32 1481 32 4147 Fragments arranged by size: 4147 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5692 PsiI TTA'TAA Cuts at: 3262 3262 Size: 5692 PstI C_TGCA'G Cuts at: 830 2916 3044 830 Size: 2086 128 3478 Fragments arranged by size: 3478 2086 128 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5692 SacI G_AGCT'C Cuts at: 2502 2502 Size: 5692 SalI G'TCGA_C Cuts at: 1936 3034 1936 Size: 1098 4594 SbfI CC_TGCA'GG Cuts at: 3044 3044 Size: 5692 ScaI AGT'ACT Cuts at: 3940 5652 3940 Size: 1712 3980 SfcI C'TryA_G Cuts at: 826 1045 2503 2653 2912 2941 3040 4175 4856 Size: 219 1458 150 259 29 99 1135 681 Cuts at: 4856 5047 826 Size: 191 1471 Fragments arranged by size: 1471 1458 1135 681 259 219 191 150 99 29 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5692 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5692 SmaI CCC'GGG Cuts at: 1872 3019 1872 Size: 1147 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3796 4697 4959 820 Size: 197 926 1853 901 262 1553 Fragments arranged by size: 1853 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3483 493 Size: 2990 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5692 SphI G_CATG'C Cuts at: 1132 3050 1132 Size: 1918 3774 SspI AAT'ATT Cuts at: 5 52 3616 5 Size: 47 3564 2081 Fragments arranged by size: 3564 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5692 StyI C'CwwG_G Cuts at: 513 1065 2004 2264 2823 3131 513 Size: 552 939 260 559 308 3074 Fragments arranged by size: 3074 939 559 552 308 260 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3719 3736 3889 4074 4519 325 Size: 1255 2139 17 153 185 445 1498 Fragments arranged by size: 2139 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3938 5650 96 Size: 274 80 33 51 1443 1961 1712 138 Fragments arranged by size: 1961 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5652 5684 5652 Size: 32 5660 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5692 XbaI T'CTAG_A Cuts at: 3028 3028 Size: 5692 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2548 2548 Size: 5692 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5692 XmaI C'CCGG_G Cuts at: 1870 3017 1870 Size: 1147 4545 XmnI GAAnn'nnTTC Cuts at: 2708 3821 2708 Size: 1113 4579 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3500 276 Size: 53 83 186 936 1966 2468 Fragments arranged by size: 2468 1966 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI