Restriction map for
pFN21AE0120
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflIII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII BamHI
BanI BbeI BbsI BcgI BciVI
BclI BglI BlpI Bpu1102I BpuAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KasI KpnI
Ksp632I KspI MaeIII MamI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI TfiI Tsp45I Tsp509I Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcyI 766 GACGCC G[GA]^CG[CT]C
AfaI 609 GTAC GT^AC
AflII 811 CTTAAG C^TTAAG
ApaI 160 GGGCCC GGGCC^C
AvaI 505 CCCGAG C^[CT]CG[GA]G
AvrII 186 CCTAGG C^CTAGG
BalI 2 TGGCCA TGG^CCA
BbrPI 727 CACGTG CAC^GTG
BfaI 187 CTAG C^TAG
BfrI 811 CTTAAG C^TTAAG
BglII 821 AGATCT A^GATCT
BlnI 186 CCTAGG C^CTAGG
BsaAI 727 CACGTG [CT]AC^GT[GA]
BsaHI 766 GACGCC G[GA]^CG[CT]C
BsmAI 787 GTCTC GTCTC(1/5)
BsmBI 786 CGTCTC CGTCTC(1/5)
BsmFI 389 GGGAC GGGAC(10/14)
BsoBI 505 CCCGAG C^[CT]CG[GA]G
BsrBI 31 GAGCGG CCGCTC(-3/-3)
BsrFI 473 ACCGGC [GA]^CCGG[CT]
BstAPI 618 GCATGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 5 CCACCGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 473 ACCGGC [GA]^CCGG[CT]
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoT14I 186 CCTAGG C^C[AT][AT]GG
Hin1I 766 GACGCC G[GA]^CG[CT]C
HincII 568 GTCAAC GT[CT]^[GA]AC
HindII 568 GTCAAC GT[CT]^[GA]AC
HinfI 225 GACTC G^A[ACGT]TC
MaeI 187 CTAG C^TAG
MboII 267 GAAGA GAAGA(8/7)
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MseI 812 TTAA T^TAA
NspI 618 GCATGC [GA]CATG^[CT]
PflMI 836 CCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 225 GACTC GAGTC(4/5)
PmaCI 727 CACGTG CAC^GTG
PmlI 727 CACGTG CAC^GTG
PpuMI 422 AGGTCCT [GA]G^G[AT]CC[CT]
RsaI 609 GTAC GT^AC
SexAI 206 ACCAGGT A^CC[AT]GGT
SphI 618 GCATGC GCATG^C
StuI 119 AGGCCT AGG^CCT
StyI 186 CCTAGG C^C[AT][AT]GG
TaqI 695 TCGA T^CGA
Tru9I 812 TTAA T^TAA
TthHB8I 695 TCGA T^CGA
Van91I 836 CCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 574 GTCTAC GT^[AC][GT]AC
775 GTCTAC
AccII 530 CGCG CG^CG
666 CGCG
Alw44I 79 GTGCAC G^TGCAC
730 GTGCAC
AlwI 336 GATCC GGATC(4/5)
750 GATCC
ApaLI 79 GTGCAC G^TGCAC
730 GTGCAC
AspHI 79 GTGCAC G[AT]GC[AT]^C
730 GTGCAC
AvaII 390 GGACC G^G[AT]CC
423 GGTCC
BbvCI 243 CCTCAGC CCTCAGC(-5/-2)
418 GCTGAGG
BseRI 46 GAGGAG GAGGAG(10/8)
49 GAGGAG
BsgI 67 CTGCAC GTGCAG(16/14)
292 CTGCAC
BsiHKAI 79 GTGCAC G[AT]GC[AT]^C
730 GTGCAC
BspMI 374 ACCTGC ACCTGC(4/8)
560 ACCTGC
BsrI 842 ACTGG ACTGG(1/-1)
402 CCAGT
BstUI 530 CGCG CG^CG
666 CGCG
BstYI 335 AGATCC [GA]^GATC[CT]
821 AGATCT
DraII 159 GGGGCCC [GA]G^G[ACGT]CC[CT]
422 AGGTCCT
DraIII 82 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
733 CACAAAGTG
DrdI 559 GACCTGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
766 GACGCCCTGGTC
EcoNI 111 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
243 CCTCAGCCAGG
EcoO109I 159 GGGGCCC [GA]G^G[ACGT]CC[CT]
422 AGGTCCT
HaeII 415 GGCGCT [GA]GCGC^[CT]
710 AGCGCC
HgaI 439 GACGC GACGC(5/10)
766 GACGC
MaeII 572 ACGT A^CGT
728 ACGT
MflI 335 AGATCC [GA]^GATC[CT]
821 AGATCT
MslI 6 CACCGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
528 CACGCGGATG
MvnI 530 CGCG CG^CG
666 CGCG
XhoII 335 AGATCC [GA]^GATC[CT]
821 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AlwNI BanII BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TseI
TspRI