(Circular) (Six-base) MAPSORT of: pFN21AE0120.seq Check: 2369 from: 1 to: 5566 pFN21AE0120 5566 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3376 278 Size: 53 83 186 936 1840 2468 Fragments arranged by size: 2468 1840 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2580 2781 2909 1937 Size: 643 201 128 4594 Fragments arranged by size: 4594 643 201 128 Acc65I G'GTAC_C Cuts at: 2887 2887 Size: 5566 AclI AA'CG_TT Cuts at: 1563 3693 4066 1563 Size: 2130 373 3063 Fragments arranged by size: 3063 2130 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2291 3629 5013 5281 1632 Size: 659 1338 1384 268 1917 Fragments arranged by size: 1917 1384 1338 659 268 AflII C'TTAA_G Cuts at: 820 1017 2816 820 Size: 197 1799 3570 Fragments arranged by size: 3570 1799 197 AflIII A'CryG_T Cuts at: 1203 4465 5442 1203 Size: 3262 977 1327 Fragments arranged by size: 3262 1327 977 AgeI A'CCGG_T Cuts at: 4397 4397 Size: 5566 AhdI GACnn_n'nnGTC Cuts at: 4295 4295 Size: 5566 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5566 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2259 1184 Size: 1075 4491 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2042 2074 2042 Size: 32 5534 AlwNI CAG_nnn'CTG Cuts at: 2296 2719 2847 3309 4881 2296 Size: 423 128 462 1572 2981 Fragments arranged by size: 2981 1572 462 423 128 ApaI G_GGCC'C Cuts at: 2169 2169 Size: 5566 ApaLI G'TGCA_C Cuts at: 2084 2735 3623 4779 2084 Size: 651 888 1156 2871 Fragments arranged by size: 2871 1156 888 651 ApoI r'AATT_y Cuts at: 1493 2875 3101 3253 5362 1493 Size: 1382 226 152 2109 1697 Fragments arranged by size: 2109 1697 1382 226 152 AseI AT'TA_AT Cuts at: 160 1032 4120 160 Size: 872 3088 1606 Fragments arranged by size: 3088 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5566 AvaI C'yCGr_G Cuts at: 1870 1943 2510 2891 1870 Size: 73 567 381 4545 Fragments arranged by size: 4545 567 381 73 AvrII C'CTAG_G Cuts at: 2191 2191 Size: 5566 BamHI G'GATC_C Cuts at: 2896 3263 2896 Size: 367 5199 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2887 4342 618 Size: 325 217 627 1100 1455 1842 Fragments arranged by size: 1842 1455 1100 627 325 217 BanII G_rGCy'C Cuts at: 1391 2153 2169 2473 2711 2885 1391 Size: 762 16 304 238 174 4072 Fragments arranged by size: 4072 762 304 238 174 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4595 BbvCI CC'TCA_GC Cuts at: 2249 2424 2249 Size: 175 5391 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3757 3791 1903 Size: 34 1820 34 3678 Fragments arranged by size: 3678 1820 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3458 4668 3458 Size: 1210 4356 BclI T'GATC_A Cuts at: 1548 1548 Size: 5566 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4177 136 Size: 107 122 71 631 605 2505 1525 Fragments arranged by size: 2505 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2826 5561 2826 Size: 2735 2831 BlpI GC'TnA_GC Cuts at: 2983 2983 Size: 5566 Bme1580I G_kGCm'C Cuts at: 1790 2088 2169 2739 3627 4783 1790 Size: 298 81 570 888 1156 2573 Fragments arranged by size: 2573 1156 888 570 298 81 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5566 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3297 4255 442 Size: 416 53 83 399 24 1880 958 1753 Fragments arranged by size: 1880 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5566 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2725 2725 Size: 5566 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2741 2824 2869 4226 1239 Size: 830 672 83 45 1357 2579 Fragments arranged by size: 2579 1357 830 672 83 45 Bpu10I CC'TnA_GC Cuts at: 2249 2424 2249 Size: 175 5391 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2102 2417 3691 4556 4854 2102 Size: 315 1274 865 298 2814 Fragments arranged by size: 2814 1274 865 315 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4229 882 Size: 617 2730 2219 Fragments arranged by size: 2730 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2734 3357 493 Size: 711 1530 623 2702 Fragments arranged by size: 2702 1530 711 623 BsaBI GATnn'nnATC Cuts at: 5560 5560 Size: 5566 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2772 3373 3755 4390 Size: 53 83 186 936 1239 601 382 635 Cuts at: 4390 275 Size: 1451 Fragments arranged by size: 1451 1239 936 635 601 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3999 4397 4671 4818 5229 3999 Size: 398 274 147 411 4336 Fragments arranged by size: 4336 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2042 2072 2813 2843 1178 Size: 30 165 30 639 30 741 30 3901 Fragments arranged by size: 3901 741 639 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 2069 1186 Size: 880 3 4683 Fragments arranged by size: 4683 880 3 BseYI C'CCAG_C Cuts at: 2659 2663 4769 5265 2659 Size: 4 2106 496 2960 Fragments arranged by size: 2960 2106 496 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2057 2282 1163 Size: 573 321 225 4447 Fragments arranged by size: 4447 573 321 225 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3016 3777 4805 1540 Size: 275 186 1015 761 1028 2301 Fragments arranged by size: 2301 1028 1015 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2088 2739 3627 3712 4783 2088 Size: 651 888 85 1071 2871 Fragments arranged by size: 2871 1071 888 651 85 BsmI GAATG_Cn' Cuts at: 3077 3170 3077 Size: 93 5473 BsmBI CGTCTCn'nnnn_ Cuts at: 2797 2797 Size: 5566 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2153 2169 2473 2711 2739 2885 Size: 399 298 65 16 304 238 28 146 Cuts at: 2885 3627 3712 4783 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 304 298 238 146 85 65 28 16 BspEI T'CCGG_A Cuts at: 5229 5229 Size: 5566 BspHI T'CATG_A Cuts at: 3455 5185 3455 Size: 1730 3836 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2388 2574 2921 844 Size: 1544 186 347 3489 Fragments arranged by size: 3489 1544 347 186 BsrBI CCG'CTC Cuts at: 1394 1823 2038 3453 1394 Size: 429 215 1415 3507 Fragments arranged by size: 3507 1415 429 215 BsrDI GCAATG_nn' Cuts at: 65 1248 4061 4235 65 Size: 1183 2813 174 1396 Fragments arranged by size: 2813 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2478 4210 4397 1700 Size: 110 668 1732 187 2869 Fragments arranged by size: 2869 1732 668 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5566 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5566 BssSI C'ACGA_G Cuts at: 3626 4638 3626 Size: 1012 4554 BstAPI GCAn_nnn'nTGC Cuts at: 794 2629 794 Size: 1835 3731 BstBI TT'CG_AA Cuts at: 4370 4370 Size: 5566 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5566 BstXI CCAn_nnnn'nTGG Cuts at: 2017 2017 Size: 5566 BstYI r'GATC_y Cuts at: 1972 2340 2826 2896 3263 3648 3665 5117 5203 Size: 368 486 70 367 385 17 1452 86 Cuts at: 5203 5215 5561 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 486 385 368 367 346 86 70 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4075 Fragments arranged by size: 4075 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4827 Fragments arranged by size: 4827 683 56 BtsI GCAGTG_nn' Cuts at: 2330 2454 3082 3876 3896 2330 Size: 124 628 794 20 4000 Fragments arranged by size: 4000 794 628 124 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5566 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5531 DraI TTT'AAA Cuts at: 2870 2870 Size: 5566 DraIII CAC_nnn'GTG Cuts at: 2092 2743 2092 Size: 651 4915 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2570 2777 4573 809 Size: 507 1254 207 1796 1802 Fragments arranged by size: 1802 1796 1254 507 207 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3013 3902 Size: 54 1425 50 275 18 177 1006 889 Cuts at: 3902 5531 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1006 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3013 1537 Size: 275 1201 4090 Fragments arranged by size: 4090 1201 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3496 1353 Size: 2143 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2048 2273 2645 4153 4527 4673 Size: 1064 138 459 225 372 1508 374 146 Cuts at: 4673 387 Size: 1280 Fragments arranged by size: 1508 1280 1064 459 374 372 225 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2291 2741 2824 2869 3629 4226 Size: 393 437 222 450 83 45 760 597 Cuts at: 4226 5013 5281 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 760 597 450 437 393 268 222 83 45 EcoNI CCTnn'n_nnAGG Cuts at: 2120 2252 2120 Size: 132 5434 EcoO109I rG'GnC_Cy Cuts at: 2165 2428 2165 Size: 263 5303 EcoRI G'AATT_C Cuts at: 2875 3253 2875 Size: 378 5188 FspI TGC'GCA Cuts at: 4072 4072 Size: 5566 HaeII r_GCGC'y Cuts at: 1678 2424 2719 4713 1678 Size: 746 295 1994 2531 Fragments arranged by size: 2531 1994 746 295 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2813 2845 4207 4239 4281 Size: 32 135 32 1241 32 1362 32 42 Cuts at: 4281 4313 1373 Size: 32 2626 Fragments arranged by size: 2626 1362 1241 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2575 2910 3156 669 Size: 988 281 637 335 246 3079 Fragments arranged by size: 3079 988 637 335 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5566 HpaI GTT'AAC Cuts at: 3156 3156 Size: 5566 KpnI G_GTAC'C Cuts at: 2891 2891 Size: 5566 MfeI C'AATT_G Cuts at: 3165 3165 Size: 5566 MluI A'CGCG_T Cuts at: 5442 5442 Size: 5566 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2586 4680 4864 1305 Size: 1281 2094 184 2007 Fragments arranged by size: 2094 2007 1281 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3567 Fragments arranged by size: 3567 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2015 2537 3524 3883 4042 518 Size: 648 849 522 987 359 159 2042 Fragments arranged by size: 2042 987 849 648 522 359 159 MspA1I CmG'CkG Cuts at: 1606 2064 2104 2199 2293 2461 2526 2620 2663 Size: 458 40 95 94 168 65 94 43 Cuts at: 2663 2982 3659 4807 5052 1606 Size: 319 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 458 319 245 168 95 94 94 65 43 40 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4075 Fragments arranged by size: 4075 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5566 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5566 NotI GC'GGCC_GC Cuts at: 3013 3013 Size: 5566 NspI r_CATG'y Cuts at: 1132 1229 2627 2924 4469 1132 Size: 97 1398 297 1545 2229 Fragments arranged by size: 2229 1545 1398 297 97 PasI CC'CwG_GG Cuts at: 1348 2687 1348 Size: 1339 4227 PciI A'CATG_T Cuts at: 4465 4465 Size: 5566 PflMI CCAn_nnn'nTGG Cuts at: 1108 2847 1108 Size: 1739 3827 PfoI T'CCnGG_A Cuts at: 2310 2343 2310 Size: 33 5533 PmeI GTTT'AAAC Cuts at: 2870 2870 Size: 5566 PmlI CAC'GTG Cuts at: 2734 2734 Size: 5566 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2042 2074 3635 3667 5148 5180 2042 Size: 32 1561 32 1481 32 2428 Fragments arranged by size: 2428 1561 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2428 2428 Size: 5566 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5566 PsiI TTA'TAA Cuts at: 3136 3136 Size: 5566 PspOMI G'GGCC_C Cuts at: 2165 2165 Size: 5566 PstI C_TGCA'G Cuts at: 830 2109 2238 2337 2460 2634 2802 2918 830 Size: 1279 129 99 123 174 168 116 3478 Fragments arranged by size: 3478 1279 174 168 129 123 116 99 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5566 PvuII CAG'CTG Cuts at: 2104 2199 2293 2461 2620 2663 2104 Size: 95 94 168 159 43 5007 Fragments arranged by size: 5007 168 159 95 94 43 SalI G'TCGA_C Cuts at: 1936 2908 1936 Size: 972 4594 SbfI CC_TGCA'GG Cuts at: 2918 2918 Size: 5566 ScaI AGT'ACT Cuts at: 3814 5526 3814 Size: 1712 3854 SexAI A'CCwGG_T Cuts at: 2211 2211 Size: 5566 SfcI C'TryA_G Cuts at: 826 1045 2105 2234 2333 2456 2630 2798 2854 Size: 219 1060 129 99 123 174 168 56 Cuts at: 2854 2914 4049 4730 4921 826 Size: 60 1135 681 191 1471 Fragments arranged by size: 1471 1135 1060 681 219 191 174 168 129 123 99 60 56 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5566 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5566 SmaI CCC'GGG Cuts at: 1872 2893 1872 Size: 1021 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2117 2432 2816 3670 4571 4833 Size: 197 926 174 315 384 854 901 262 Cuts at: 4833 820 Size: 1553 Fragments arranged by size: 1553 926 901 854 384 315 262 197 174 SnaBI TAC'GTA Cuts at: 493 3357 493 Size: 2864 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5566 SphI G_CATG'C Cuts at: 1132 2627 2924 1132 Size: 1495 297 3774 Fragments arranged by size: 3774 1495 297 SspI AAT'ATT Cuts at: 5 52 3490 5 Size: 47 3438 2081 Fragments arranged by size: 3438 2081 47 StuI AGG'CCT Cuts at: 1517 2126 1517 Size: 609 4957 StyI C'CwwG_G Cuts at: 513 1065 2004 2191 3005 513 Size: 552 939 187 814 3074 Fragments arranged by size: 3074 939 814 552 187 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3593 3610 3763 3948 4393 325 Size: 1255 2013 17 153 185 445 1498 Fragments arranged by size: 2013 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3812 5524 96 Size: 274 80 33 51 1443 1835 1712 138 Fragments arranged by size: 1835 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2309 2341 5526 5558 2309 Size: 32 3185 32 2317 Fragments arranged by size: 3185 2317 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5566 XbaI T'CTAG_A Cuts at: 2902 2902 Size: 5566 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5566 XmaI C'CCGG_G Cuts at: 1870 2891 1870 Size: 1021 4545 XmnI GAAnn'nnTTC Cuts at: 3695 3695 Size: 5566 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3374 276 Size: 53 83 186 936 1840 2468 Fragments arranged by size: 2468 1840 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI