Restriction map for
pFN21AE0120
(Linear) (Six-base) MAPSORT of: KIEE0120.seq Check: 5346 from: 1 to: 861
KIEE0120.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 0 575 776 861
Size: 575 201 85
Fragments arranged by size:
575 201 85
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 861
Size: 286 575
AflII C'TTAA_G
Cuts at: 0 811 861
Size: 811 50
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 254 861
Size: 254 607
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 37 69 861
Size: 37 32 792
Fragments arranged by size:
792 37 32
AlwNI CAG_nnn'CTG
Cuts at: 0 291 714 842 861
Size: 291 423 128 19
Fragments arranged by size:
423 291 128 19
ApaI G_GGCC'C
Cuts at: 0 164 861
Size: 164 697
ApaLI G'TGCA_C
Cuts at: 0 79 730 861
Size: 79 651 131
Fragments arranged by size:
651 131 79
AvaI C'yCGr_G
Cuts at: 0 505 861
Size: 505 356
AvrII C'CTAG_G
Cuts at: 0 186 861
Size: 186 675
BanII G_rGCy'C
Cuts at: 0 148 164 468 706 861
Size: 148 16 304 238 155
Fragments arranged by size:
304 238 155 148 16
BbvCI CC'TCA_GC
Cuts at: 0 244 419 861
Size: 244 175 442
Fragments arranged by size:
442 244 175
BglII A'GATC_T
Cuts at: 0 821 861
Size: 821 40
Bme1580I G_kGCm'C
Cuts at: 0 83 164 734 861
Size: 83 81 570 127
Fragments arranged by size:
570 127 83 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 720 861
Size: 720 141
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 736 819 861
Size: 64 672 83 42
Fragments arranged by size:
672 83 64 42
Bpu10I CC'TnA_GC
Cuts at: 0 244 419 861
Size: 244 175 442
Fragments arranged by size:
442 244 175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 412 861
Size: 97 315 449
Fragments arranged by size:
449 315 97
BsaAI yAC'GTr
Cuts at: 0 729 861
Size: 729 132
BsaHI Gr'CG_yC
Cuts at: 0 767 861
Size: 767 94
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 37 67 808 838 861
Size: 37 30 741 30 23
Fragments arranged by size:
741 37 30 30 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 64 861
Size: 61 3 797
Fragments arranged by size:
797 61 3
BseYI C'CCAG_C
Cuts at: 0 654 658 861
Size: 654 4 203
Fragments arranged by size:
654 203 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 277 861
Size: 52 225 584
Fragments arranged by size:
584 225 52
BsiHKAI G_wGCw'C
Cuts at: 0 83 734 861
Size: 83 651 127
Fragments arranged by size:
651 127 83
BsmBI CGTCTCn'nnnn_
Cuts at: 0 792 861
Size: 792 69
Bsp1286I G_dGCh'C
Cuts at: 0 83 148 164 468 706 734 861
Size: 83 65 16 304 238 28 127
Fragments arranged by size:
304 238 127 83 65 28 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 383 569 861
Size: 383 186 292
Fragments arranged by size:
383 292 186
BsrBI CCG'CTC
Cuts at: 0 33 861
Size: 33 828
BsrFI r'CCGG_y
Cuts at: 0 473 861
Size: 473 388
BstAPI GCAn_nnn'nTGC
Cuts at: 0 624 861
Size: 624 237
BstXI CCAn_nnnn'nTGG
Cuts at: 0 12 861
Size: 12 849
BstYI r'GATC_y
Cuts at: 0 335 821 861
Size: 335 486 40
Fragments arranged by size:
486 335 40
BtsI GCAGTG_nn'
Cuts at: 0 325 449 861
Size: 325 124 412
Fragments arranged by size:
412 325 124
DraIII CAC_nnn'GTG
Cuts at: 0 87 738 861
Size: 87 651 123
Fragments arranged by size:
651 123 87
DrdI GACnn_nn'nnGTC
Cuts at: 0 565 772 861
Size: 565 207 89
Fragments arranged by size:
565 207 89
EaeI y'GGCC_r
Cuts at: 0 2 861
Size: 2 859
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 43 268 640 861
Size: 43 225 372 221
Fragments arranged by size:
372 225 221 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 286 736 819 861
Size: 64 222 450 83 42
Fragments arranged by size:
450 222 83 64 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 115 247 861
Size: 115 132 614
Fragments arranged by size:
614 132 115
EcoO109I rG'GnC_Cy
Cuts at: 0 160 423 861
Size: 160 263 438
Fragments arranged by size:
438 263 160
HaeII r_GCGC'y
Cuts at: 0 419 714 861
Size: 419 295 147
Fragments arranged by size:
419 295 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 808 840 861
Size: 808 32 21
Fragments arranged by size:
808 32 21
HincII GTy'rAC
Cuts at: 0 570 861
Size: 570 291
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 861
Size: 581 280
MscI TGG'CCA
Cuts at: 0 4 861
Size: 4 857
MslI CAynn'nnrTG
Cuts at: 0 10 532 861
Size: 10 522 329
Fragments arranged by size:
522 329 10
MspA1I CmG'CkG
Cuts at: 0 59 99 194 288 456 521 615 658
Size: 59 40 95 94 168 65 94 43
Cuts at: 658 861
Size: 203
Fragments arranged by size:
203 168 95 94 94 65 59 43
40
NspI r_CATG'y
Cuts at: 0 622 861
Size: 622 239
PasI CC'CwG_GG
Cuts at: 0 682 861
Size: 682 179
PflMI CCAn_nnn'nTGG
Cuts at: 0 842 861
Size: 842 19
PfoI T'CCnGG_A
Cuts at: 0 305 338 861
Size: 305 33 523
Fragments arranged by size:
523 305 33
PmlI CAC'GTG
Cuts at: 0 729 861
Size: 729 132
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 37 69 861
Size: 37 32 792
Fragments arranged by size:
792 37 32
PpuMI rG'GwC_Cy
Cuts at: 0 423 861
Size: 423 438
PspOMI G'GGCC_C
Cuts at: 0 160 861
Size: 160 701
PstI C_TGCA'G
Cuts at: 0 104 233 332 455 629 797 861
Size: 104 129 99 123 174 168 64
Fragments arranged by size:
174 168 129 123 104 99 64
PvuII CAG'CTG
Cuts at: 0 99 194 288 456 615 658 861
Size: 99 95 94 168 159 43 203
Fragments arranged by size:
203 168 159 99 95 94 43
SexAI A'CCwGG_T
Cuts at: 0 206 861
Size: 206 655
SfcI C'TryA_G
Cuts at: 0 100 229 328 451 625 793 849 861
Size: 100 129 99 123 174 168 56 12
Fragments arranged by size:
174 168 129 123 100 99 56 12
SmlI C'TyrA_G
Cuts at: 0 112 427 811 861
Size: 112 315 384 50
Fragments arranged by size:
384 315 112 50
SphI G_CATG'C
Cuts at: 0 622 861
Size: 622 239
StuI AGG'CCT
Cuts at: 0 121 861
Size: 121 740
StyI C'CwwG_G
Cuts at: 0 186 861
Size: 186 675
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 304 336 861
Size: 304 32 525
Fragments arranged by size:
525 304 32
Enzymes that do cut:
AccI AcuI AflII AlfI AloI AlwNI ApaI ApaLI
AvaI AvrII BanII BbvCI BglII Bme1580I BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI
BstYI BtsI DraIII DrdI EaeI EciI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII MmeI MscI MslI MspA1I
NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI
PstI PvuII SexAI SfcI SmlI SphI StuI StyI
TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI ApoI AscI AseI AsiSI BaeI BamHI BanI
BbeI BbsI BcgI BciVI BclI BfrBI BglI BlpI
BmgBI BmrI BmtI BsaI BsaBI BsaWI BsiEI BsiWI
BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
EagI EarI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI TaqII TatI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI