(Circular) (Six-base) MAPSORT of: pFN21AB9865.seq Check: 4361 from: 1 to: 6001 pFN21AB9865 6001 bp With 182 enzymes: SgfI * February 27, 2009 14:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2115 2371 2115 Size: 256 5745 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3811 278 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3344 1937 Size: 1407 4594 Acc65I G'GTAC_C Cuts at: 3322 3322 Size: 6001 AclI AA'CG_TT Cuts at: 1563 4128 4501 1563 Size: 2565 373 3063 Fragments arranged by size: 3063 2565 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4064 5448 5716 1632 Size: 2432 1384 268 1917 Fragments arranged by size: 2432 1917 1384 268 AfeI AGC'GCT Cuts at: 2201 2201 Size: 6001 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5804 AflIII A'CryG_T Cuts at: 1203 2214 4900 5877 1203 Size: 1011 2686 977 1327 Fragments arranged by size: 2686 1327 1011 977 AgeI A'CCGG_T Cuts at: 4832 4832 Size: 6001 AhdI GACnn_n'nnGTC Cuts at: 2191 4730 2191 Size: 2539 3462 AleI CACnn'nnGTG Cuts at: 1166 2228 1166 Size: 1062 4939 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6001 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3083 3115 3083 Size: 32 5969 AlwNI CAG_nnn'CTG Cuts at: 2584 2866 3744 5316 2584 Size: 282 878 1572 3269 Fragments arranged by size: 3269 1572 878 282 ApaLI G'TGCA_C Cuts at: 2111 2384 4058 5214 2111 Size: 273 1674 1156 2898 Fragments arranged by size: 2898 1674 1156 273 ApoI r'AATT_y Cuts at: 1493 3310 3536 3688 5797 1493 Size: 1817 226 152 2109 1697 Fragments arranged by size: 2109 1817 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4555 160 Size: 872 3523 1606 Fragments arranged by size: 3523 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 AvaI C'yCGr_G Cuts at: 1870 1943 3326 1870 Size: 73 1383 4545 Fragments arranged by size: 4545 1383 73 BamHI G'GATC_C Cuts at: 3331 3698 3331 Size: 367 5634 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2084 2127 2537 3052 3322 Size: 325 217 627 297 43 410 515 270 Cuts at: 3322 4777 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 515 410 325 297 270 217 43 BanII G_rGCy'C Cuts at: 1391 2991 3320 1391 Size: 1600 329 4072 Fragments arranged by size: 4072 1600 329 BbeI G_GCGC'C Cuts at: 2088 2088 Size: 6001 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2473 928 Size: 971 574 4456 Fragments arranged by size: 4456 971 574 BbvCI CC'TCA_GC Cuts at: 2349 2349 Size: 6001 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2122 2156 4192 4226 1903 Size: 34 185 34 2036 34 3678 Fragments arranged by size: 3678 2036 185 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3893 5103 3893 Size: 1210 4791 BclI T'GATC_A Cuts at: 1548 1548 Size: 6001 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2021 2546 2555 Size: 107 122 71 631 605 349 525 9 Cuts at: 2555 2609 3207 4612 136 Size: 54 598 1405 1525 Fragments arranged by size: 1525 1405 631 605 598 525 349 122 107 71 54 9 BglII A'GATC_T Cuts at: 5996 5996 Size: 6001 BlpI GC'TnA_GC Cuts at: 3418 3418 Size: 6001 Bme1580I G_kGCm'C Cuts at: 1790 2115 2388 2471 2578 3057 4062 5218 1790 Size: 325 273 83 107 479 1005 1156 2573 Fragments arranged by size: 2573 1156 1005 479 325 273 107 83 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6001 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2491 3732 4690 Size: 416 53 83 399 24 1074 1241 958 Cuts at: 4690 442 Size: 1753 Fragments arranged by size: 1753 1241 1074 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6001 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3073 3073 Size: 6001 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2360 2888 3230 3287 4661 1239 Size: 1121 528 342 57 1374 2579 Fragments arranged by size: 2579 1374 1121 528 342 57 Bpu10I CC'TnA_GC Cuts at: 2349 3152 2349 Size: 803 5198 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4126 4991 5289 4126 Size: 865 298 4838 Fragments arranged by size: 4838 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3177 4664 882 Size: 617 1678 1487 2219 Fragments arranged by size: 2219 1678 1487 617 BsaAI yAC'GTr Cuts at: 493 1204 3792 493 Size: 711 2588 2702 Fragments arranged by size: 2702 2588 711 BsaBI GATnn'nnATC Cuts at: 2753 5995 2753 Size: 3242 2759 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2085 3808 4190 4825 Size: 53 83 186 936 552 1723 382 635 Cuts at: 4825 275 Size: 1451 Fragments arranged by size: 1723 1451 936 635 552 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4434 4832 5106 5253 5664 4434 Size: 398 274 147 411 4771 Fragments arranged by size: 4771 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2714 2744 3083 3113 1178 Size: 30 165 30 1311 30 339 30 4066 Fragments arranged by size: 4066 1311 339 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 2477 2735 3179 1186 Size: 880 411 258 444 4008 Fragments arranged by size: 4008 880 444 411 258 BseYI C'CCAG_C Cuts at: 5204 5700 5204 Size: 496 5505 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2048 1163 Size: 573 312 5116 Fragments arranged by size: 5116 573 312 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3451 4212 5240 1540 Size: 275 186 1450 761 1028 2301 Fragments arranged by size: 2301 1450 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2115 2388 2997 4062 4147 5218 2115 Size: 273 609 1065 85 1071 2898 Fragments arranged by size: 2898 1071 1065 609 273 85 BsmI GAATG_Cn' Cuts at: 3512 3605 3512 Size: 93 5908 BsmBI CGTCTCn'nnnn_ Cuts at: 2414 2864 2414 Size: 450 5551 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2388 2471 2578 2991 2997 3057 Size: 399 325 273 83 107 413 6 60 Cuts at: 3057 3320 4062 4147 5218 1391 Size: 263 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 413 399 325 273 263 107 85 83 60 6 BspEI T'CCGG_A Cuts at: 5664 5664 Size: 6001 BspHI T'CATG_A Cuts at: 2529 3890 5620 2529 Size: 1361 1730 2910 Fragments arranged by size: 2910 1730 1361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2115 2371 2664 3356 844 Size: 1271 256 293 692 3489 Fragments arranged by size: 3489 1271 692 293 256 BsrBI CCG'CTC Cuts at: 1394 1823 2842 3888 1394 Size: 429 1019 1046 3507 Fragments arranged by size: 3507 1046 1019 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4496 4670 65 Size: 1183 3248 174 1396 Fragments arranged by size: 3248 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2344 2856 4645 4832 1700 Size: 110 534 512 1789 187 2869 Fragments arranged by size: 2869 1789 534 512 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6001 BssHII G'CGCG_C Cuts at: 1925 2689 2691 1925 Size: 764 2 5235 Fragments arranged by size: 5235 764 2 BssSI C'ACGA_G Cuts at: 4061 5073 4061 Size: 1012 4989 BstAPI GCAn_nnn'nTGC Cuts at: 794 2631 794 Size: 1837 4164 BstBI TT'CG_AA Cuts at: 4805 4805 Size: 6001 BstEII G'GTnAC_C Cuts at: 1184 2071 2333 2651 2713 2975 1184 Size: 887 262 318 62 262 4210 Fragments arranged by size: 4210 887 318 262 262 62 BstXI CCAn_nnnn'nTGG Cuts at: 2230 3254 2230 Size: 1024 4977 BstYI r'GATC_y Cuts at: 1972 2964 3082 3331 3698 4083 4100 5552 5638 Size: 992 118 249 367 385 17 1452 86 Cuts at: 5638 5650 5996 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 992 385 367 346 249 118 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2010 2604 2708 513 Size: 552 945 594 104 3806 Fragments arranged by size: 3806 945 594 552 104 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2302 487 Size: 683 56 1076 4186 Fragments arranged by size: 4186 1076 683 56 BtsI GCAGTG_nn' Cuts at: 3517 4311 4331 3517 Size: 794 20 5187 Fragments arranged by size: 5187 794 20 ClaI AT'CG_AT Cuts at: 1554 2757 1554 Size: 1203 4798 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5966 DraI TTT'AAA Cuts at: 3305 3305 Size: 6001 DraIII CAC_nnn'GTG Cuts at: 2333 2372 2674 2333 Size: 39 302 5660 Fragments arranged by size: 5660 302 39 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5008 809 Size: 507 3692 1802 Fragments arranged by size: 3692 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2155 2475 Size: 54 1425 50 275 18 183 142 320 Cuts at: 2475 2547 2955 3193 3448 4337 5966 8 Size: 72 408 238 255 889 1629 43 Fragments arranged by size: 1629 1425 889 408 320 275 255 238 183 142 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3448 1537 Size: 275 1636 4090 Fragments arranged by size: 4090 1636 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2414 2849 3002 3931 1353 Size: 1061 435 153 929 3423 Fragments arranged by size: 3423 1061 929 435 153 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3191 4588 4962 5108 387 Size: 1064 138 1602 1397 374 146 1280 Fragments arranged by size: 1602 1397 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2360 2888 3230 3287 4064 4661 5448 Size: 393 728 528 342 57 777 597 787 Cuts at: 5448 5716 1239 Size: 268 1524 Fragments arranged by size: 1524 787 777 728 597 528 393 342 268 57 EcoO109I rG'GnC_Cy Cuts at: 2776 2863 3262 2776 Size: 87 399 5515 Fragments arranged by size: 5515 399 87 EcoRI G'AATT_C Cuts at: 3310 3688 3310 Size: 378 5623 FspI TGC'GCA Cuts at: 2660 4507 2660 Size: 1847 4154 HaeII r_GCGC'y Cuts at: 1678 2088 2203 5148 1678 Size: 410 115 2945 2531 Fragments arranged by size: 2945 2531 410 115 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2177 2209 4642 4674 4716 Size: 32 135 32 605 32 2433 32 42 Cuts at: 4716 4748 1373 Size: 32 2626 Fragments arranged by size: 2626 2433 605 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3345 3591 669 Size: 988 281 1407 246 3079 Fragments arranged by size: 3079 1407 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6001 HpaI GTT'AAC Cuts at: 3591 3591 Size: 6001 KasI G'GCGC_C Cuts at: 2084 2084 Size: 6001 KpnI G_GTAC'C Cuts at: 3326 3326 Size: 6001 MfeI C'AATT_G Cuts at: 3600 3600 Size: 6001 MluI A'CGCG_T Cuts at: 5877 5877 Size: 6001 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5115 5299 1305 Size: 3810 184 2007 Fragments arranged by size: 3810 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2157 2549 2957 10 Size: 54 1425 343 325 392 408 3054 Fragments arranged by size: 3054 1425 408 392 343 325 54 MslI CAynn'nnrTG Cuts at: 518 1166 2228 2321 2489 2759 2924 3959 4318 Size: 648 1062 93 168 270 165 1035 359 Cuts at: 4318 4477 518 Size: 159 2042 Fragments arranged by size: 2042 1062 1035 648 359 270 168 165 159 93 MspA1I CmG'CkG Cuts at: 1606 2012 2241 2710 2740 3069 3274 3417 4094 Size: 406 229 469 30 329 205 143 677 Cuts at: 4094 5242 5487 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 469 406 329 245 229 205 143 30 NaeI GCC'GGC Cuts at: 2346 2858 2346 Size: 512 5489 NarI GG'CG_CC Cuts at: 2085 2085 Size: 6001 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5449 NdeI CA'TA_TG Cuts at: 387 387 Size: 6001 NgoMIV G'CCGG_C Cuts at: 2344 2856 2344 Size: 512 5489 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6001 NotI GC'GGCC_GC Cuts at: 3448 3448 Size: 6001 NspI r_CATG'y Cuts at: 1132 1229 2218 2254 3359 4904 1132 Size: 97 989 36 1105 1545 2229 Fragments arranged by size: 2229 1545 1105 989 97 36 PasI CC'CwG_GG Cuts at: 1348 3111 1348 Size: 1763 4238 PciI A'CATG_T Cuts at: 2214 4900 2214 Size: 2686 3315 PflMI CCAn_nnn'nTGG Cuts at: 1108 2674 1108 Size: 1566 4435 PfoI T'CCnGG_A Cuts at: 2898 3264 2898 Size: 366 5635 PmeI GTTT'AAAC Cuts at: 3305 3305 Size: 6001 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3083 3115 4070 4102 5583 5615 3083 Size: 32 955 32 1481 32 3469 Fragments arranged by size: 3469 1481 955 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2863 3262 2863 Size: 399 5602 PshAI GACnn'nnGTC Cuts at: 1613 3289 1613 Size: 1676 4325 PsiI TTA'TAA Cuts at: 3571 3571 Size: 6001 PstI C_TGCA'G Cuts at: 830 3074 3353 830 Size: 2244 279 3478 Fragments arranged by size: 3478 2244 279 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6001 SacII CC_GC'GG Cuts at: 2013 2711 2013 Size: 698 5303 SalI G'TCGA_C Cuts at: 1936 3343 1936 Size: 1407 4594 SapI GCTCTTCn'nnn_ Cuts at: 2849 3002 2849 Size: 153 5848 SbfI CC_TGCA'GG Cuts at: 3353 3353 Size: 6001 ScaI AGT'ACT Cuts at: 4249 5961 4249 Size: 1712 4289 SexAI A'CCwGG_T Cuts at: 2067 2067 Size: 6001 SfcI C'TryA_G Cuts at: 826 1045 2032 3070 3280 3349 4484 5165 5356 Size: 219 987 1038 210 69 1135 681 191 Cuts at: 5356 826 Size: 1471 Fragments arranged by size: 1471 1135 1038 987 681 219 210 191 69 SfiI GGCCn_nnn'nGGCC Cuts at: 2021 2021 Size: 6001 SfoI GGC'GCC Cuts at: 2086 2086 Size: 6001 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6001 SmaI CCC'GGG Cuts at: 1872 3328 1872 Size: 1456 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4105 5006 5268 820 Size: 197 926 2162 901 262 1553 Fragments arranged by size: 2162 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3792 493 Size: 3299 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6001 SphI G_CATG'C Cuts at: 1132 3359 1132 Size: 2227 3774 SspI AAT'ATT Cuts at: 5 52 3925 5 Size: 47 3873 2081 Fragments arranged by size: 3873 2081 47 StuI AGG'CCT Cuts at: 1517 3210 1517 Size: 1693 4308 StyI C'CwwG_G Cuts at: 513 1065 3440 513 Size: 552 2375 3074 Fragments arranged by size: 3074 2375 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2948 4028 4045 4198 4383 4828 325 Size: 1255 1368 1080 17 153 185 445 1498 Fragments arranged by size: 1498 1368 1255 1080 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2730 3012 4247 Size: 274 80 33 51 1443 753 282 1235 Cuts at: 4247 5959 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1235 753 282 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5961 5993 5961 Size: 32 5969 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6001 XbaI T'CTAG_A Cuts at: 3337 3337 Size: 6001 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6001 XmaI C'CCGG_G Cuts at: 1870 3326 1870 Size: 1456 4545 XmnI GAAnn'nnTTC Cuts at: 3098 4130 3098 Size: 1032 4969 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3809 276 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SrfI SwaI XcmI