################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## Acc65I AccII AccIII AclI AflII AgeI AhdI AlwNI AocI ApaI AscI AseI AsnI Asp718I AspEI AviII AvrII BbrPI BbsI BbvCI BcgI BfrI BglI BlnI BlpI Bpu1102I BpuAI BseAI BsiEI BsiWI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoRI FseI FspI HindIII KpnI KspI MluI MroI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SgrAI SmlI SpeI SphI Sse8387I SspI StuI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AatII 1800 GACGTC GACGT^C Alw44I 2652 GTGCAC G^TGCAC Aor51HI 359 AGCGCT AGC^GCT ApaLI 2652 GTGCAC G^TGCAC Asp700I 911 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 556 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2582 CCCGGG C^[CT]CG[GA]G BalI 2424 TGGCCA TGG^CCA BanII 2980 GGGCTC G[GA]GC[CT]^C BbeI 2470 GGCGCC GGCGC^C BciVI 2507 GTATCC GTATCC(6/5) BclI 2132 TGATCA T^GATCA BglII 167 AGATCT A^GATCT BsaBI 1926 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1473 ACCGGA [AT]^CCGG[AT] BseRI 163 GAGGAG GAGGAG(10/8) BsgI 294 CTGCAC GTGCAG(16/14) BsmBI 2554 GAGACG CGTCTC(1/5) BsoBI 2582 CCCGGG C^[CT]CG[GA]G Bsp1407I 1848 TGTACA T^GTACA BsrGI 1848 TGTACA T^GTACA BstEII 145 GGTGACC G^GT[ACGT]ACC BstPI 145 GGTGACC G^GT[ACGT]ACC EaeI 2424 TGGCCA [CT]^GGCC[GA] Eco47III 359 AGCGCT AGC^GCT EcoNI 1261 CCTGTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 145 GGTGACC G^GT[ACGT]ACC EcoRV 529 GATATC GAT^ATC EcoT22I 334 ATGCAT ATGCA^T FauI 1859 GCGGG CCCGC(4/6) FbaI 2132 TGATCA T^GATCA HgaI 328 GACGC GACGC(5/10) HpaI 840 GTTAAC GTT^AAC KasI 2470 GGCGCC G^GCGCC MamI 1926 GATTGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2424 TGGCCA TGG^CCA MscI 2424 TGGCCA TGG^CCA NarI 2470 GGCGCC GG^CGCC NsiI 334 ATGCAT ATGCA^T PflFI 556 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC SapI 1219 GAAGAGC GCTCTTC(1/4) SfcI 710 CTACAG C^T[GA][CT]AG SfoI 2470 GGCGCC GGC^GCC SmaI 2582 CCCGGG CCC^GGG SnaBI 1465 TACGTA TAC^GTA SspBI 1848 TGTACA T^GTACA Tth111I 556 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1412 CCATTGATGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 2582 CCCGGG C^CCGGG XmaI 2582 CCCGGG C^CCGGG XmnI 911 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### BfaI 1153 CTAG C^TAG 2047 CTAG BsaAI 1328 TACGTG [CT]AC^GT[GA] 1465 TACGTA BsmI 63 GAATGC GAATGC(1/-1) 2183 GCATTC BsrDI 1716 CATTGC GCAATG(2/0) 2144 GCAATG BstBI 1238 TTCGAA TT^CGAA 1646 TTCGAA DraII 373 GGGACCC [GA]G^G[ACGT]CC[CT] 2006 AGGACCT DsaI 534 CCGTGG C^C[GA][CT]GG 897 CCGTGG EarI 349 CTCTTC CTCTTC(1/4) 1219 GAAGAG EcoO109I 373 GGGACCC [GA]G^G[ACGT]CC[CT] 2006 AGGACCT EcoT14I 379 CCAAGG C^C[AT][AT]GG 1200 CCTTGG HincII 840 GTTAAC GT[CT]^[GA]AC 1140 GTTGAC HindII 840 GTTAAC GT[CT]^[GA]AC 1140 GTTGAC Ksp632I 349 CTCTTC CTCTTC(1/4) 1219 GAAGAG MaeI 1153 CTAG C^TAG 2047 CTAG MfeI 830 CAATTG C^AATTG 2696 CAATTG MslI 2541 CATAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2632 CATAGACGTG MunI 830 CAATTG C^AATTG 2696 CAATTG NspV 1238 TTCGAA TT^CGAA 1646 TTCGAA PflMI 315 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 379 CCAAGGAGTGG PpuMI 373 GGGACCC [GA]G^G[AT]CC[CT] 2006 AGGACCT PvuII 368 CAGCTG CAG^CTG 2228 CAGCTG SfuI 1238 TTCGAA TT^CGAA 1646 TTCGAA StyI 379 CCAAGG C^C[AT][AT]GG 1200 CCTTGG Van91I 315 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 379 CCAAGGAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AflIII AluI AlwI ApoI AspHI AvaII BamHI BanI BbvI BcnI BmyI BpmI BsaHI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII