(Circular) (Six-base) MAPSORT of: pFN21AB4222.seq Check: 5177 from: 1 to: 7726 pFN21AB4222 7726 bp With 182 enzymes: SgfI * February 27, 2009 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3809 5536 278 Size: 53 83 186 936 2273 1727 2468 Fragments arranged by size: 2468 2273 1727 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2454 3748 3756 3809 5069 1937 Size: 517 1294 8 53 1260 4594 Fragments arranged by size: 4594 1294 1260 517 53 8 Acc65I G'GTAC_C Cuts at: 5047 5047 Size: 7726 AclI AA'CG_TT Cuts at: 1563 5853 6226 1563 Size: 4290 373 3063 Fragments arranged by size: 4290 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2579 4361 4424 5789 7173 7441 1632 Size: 947 1782 63 1365 1384 268 1917 Fragments arranged by size: 1917 1782 1384 1365 947 268 63 AfeI AGC'GCT Cuts at: 2366 2366 Size: 7726 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7529 AflIII A'CryG_T Cuts at: 1203 3334 3617 4642 6625 7602 1203 Size: 2131 283 1025 1983 977 1327 Fragments arranged by size: 2131 1983 1327 1025 977 283 AgeI A'CCGG_T Cuts at: 6557 6557 Size: 7726 AhdI GACnn_n'nnGTC Cuts at: 6455 6455 Size: 7726 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7726 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7726 AlwNI CAG_nnn'CTG Cuts at: 5469 7041 5469 Size: 1572 6154 ApaLI G'TGCA_C Cuts at: 4657 5783 6939 4657 Size: 1126 1156 5444 Fragments arranged by size: 5444 1156 1126 ApoI r'AATT_y Cuts at: 1493 2504 3161 3465 5035 5261 5413 7522 1493 Size: 1011 657 304 1570 226 152 2109 1697 Fragments arranged by size: 2109 1697 1570 1011 657 304 226 152 AseI AT'TA_AT Cuts at: 160 1032 6280 160 Size: 872 5248 1606 Fragments arranged by size: 5248 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7726 AvaI C'yCGr_G Cuts at: 1870 1943 4587 5051 1870 Size: 73 2644 464 4545 Fragments arranged by size: 4545 2644 464 73 BamHI G'GATC_C Cuts at: 2312 2944 4365 5056 5423 2312 Size: 632 1421 691 367 4615 Fragments arranged by size: 4615 1421 691 632 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4475 4909 5015 5047 6502 Size: 325 217 627 2688 434 106 32 1455 Cuts at: 6502 618 Size: 1842 Fragments arranged by size: 2688 1842 1455 627 434 325 217 106 32 BanII G_rGCy'C Cuts at: 1391 4989 5045 1391 Size: 3598 56 4072 Fragments arranged by size: 4072 3598 56 BbeI G_GCGC'C Cuts at: 4479 4479 Size: 7726 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6755 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5917 5951 1903 Size: 34 3980 34 3678 Fragments arranged by size: 3980 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4523 5618 6828 4523 Size: 1095 1210 5421 Fragments arranged by size: 5421 1210 1095 BclI T'GATC_A Cuts at: 1548 4137 1548 Size: 2589 5137 BfrBI ATG'CAT Cuts at: 2341 2341 Size: 7726 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6337 136 Size: 107 122 71 631 605 4665 1525 Fragments arranged by size: 4665 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2172 7721 2172 Size: 5549 2177 BlpI GC'TnA_GC Cuts at: 5143 5143 Size: 7726 Bme1580I G_kGCm'C Cuts at: 1790 4661 4912 5787 6943 1790 Size: 2871 251 875 1156 2573 Fragments arranged by size: 2871 2573 1156 875 251 BmgBI CAC'GTC Cuts at: 1318 2304 4563 4643 1318 Size: 986 2259 80 4401 Fragments arranged by size: 4401 2259 986 80 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5457 6415 442 Size: 416 53 83 399 24 4040 958 1753 Fragments arranged by size: 4040 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7726 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2303 3050 3171 3570 3992 4193 4391 4577 Size: 1064 747 121 399 422 201 198 186 Cuts at: 4577 4646 4772 6386 1239 Size: 69 126 1614 2579 Fragments arranged by size: 2579 1614 1064 747 422 399 201 198 186 126 121 69 Bpu10I CC'TnA_GC Cuts at: 4878 4878 Size: 7726 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5851 6716 7014 5851 Size: 865 298 6563 Fragments arranged by size: 6563 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2052 3110 3376 6389 882 Size: 617 553 1058 266 3013 2219 Fragments arranged by size: 3013 2219 1058 617 553 266 BsaAI yAC'GTr Cuts at: 493 1204 3335 3472 5517 493 Size: 711 2131 137 2045 2702 Fragments arranged by size: 2702 2131 2045 711 137 BsaBI GATnn'nnATC Cuts at: 3935 7720 3935 Size: 3785 3941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2334 3806 4476 5533 Size: 53 83 186 936 801 1472 670 1057 Cuts at: 5533 5915 6550 275 Size: 382 635 1451 Fragments arranged by size: 1472 1451 1057 936 801 670 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3478 6159 6557 6831 6978 7389 3478 Size: 2681 398 274 147 411 3815 Fragments arranged by size: 3815 2681 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2380 2410 3525 3555 3568 Size: 30 165 30 977 30 1115 30 13 Cuts at: 3568 3598 4550 4580 1178 Size: 30 952 30 4324 Fragments arranged by size: 4324 1115 977 952 165 30 30 30 30 30 30 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2183 1186 Size: 997 6729 BseYI C'CCAG_C Cuts at: 2375 6929 7425 2375 Size: 4554 496 2676 Fragments arranged by size: 4554 2676 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2284 1163 Size: 573 548 6605 Fragments arranged by size: 6605 573 548 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5176 5937 6965 1540 Size: 275 186 3175 761 1028 2301 Fragments arranged by size: 3175 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4632 4661 4884 5787 5872 6943 4632 Size: 29 223 903 85 1071 5415 Fragments arranged by size: 5415 1071 903 223 85 29 BsmI GAATG_Cn' Cuts at: 2074 4188 5237 5330 2074 Size: 2114 1049 93 4470 Fragments arranged by size: 4470 2114 1049 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4553 4553 Size: 7726 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4632 4661 4884 4912 4989 5045 5787 Size: 399 2842 29 223 28 77 56 742 Cuts at: 5787 5872 6943 1391 Size: 85 1071 2174 Fragments arranged by size: 2842 2174 1071 742 399 223 85 77 56 29 28 BspEI T'CCGG_A Cuts at: 7389 7389 Size: 7726 BspHI T'CATG_A Cuts at: 5615 7345 5615 Size: 1730 5996 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5081 844 Size: 4237 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5613 1394 Size: 429 3790 3507 Fragments arranged by size: 3790 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3720 4156 6221 6395 65 Size: 1183 2472 436 2065 174 1396 Fragments arranged by size: 2472 2065 1396 1183 436 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6370 6557 1700 Size: 110 4560 187 2869 Fragments arranged by size: 4560 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3853 96 Size: 3757 3969 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7726 BssSI C'ACGA_G Cuts at: 5786 6798 5786 Size: 1012 6714 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7726 BstBI TT'CG_AA Cuts at: 3244 3652 6530 3244 Size: 408 2878 4440 Fragments arranged by size: 4440 2878 408 BstEII G'GTnAC_C Cuts at: 1184 2150 1184 Size: 966 6760 BstYI r'GATC_y Cuts at: 1972 2172 2312 2944 3845 4365 4812 5056 5423 Size: 200 140 632 901 520 447 244 367 Cuts at: 5423 5808 5825 7277 7363 7375 7721 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 901 632 520 447 385 367 346 244 200 140 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2539 2902 513 Size: 552 939 535 363 5337 Fragments arranged by size: 5337 939 552 535 363 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6987 Fragments arranged by size: 6987 683 56 BtsI GCAGTG_nn' Cuts at: 2139 5242 6036 6056 2139 Size: 3103 794 20 3809 Fragments arranged by size: 3809 3103 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7726 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2620 2655 3112 3147 529 Size: 35 2056 35 457 35 5108 Fragments arranged by size: 5108 2056 457 35 35 35 DraI TTT'AAA Cuts at: 5030 5030 Size: 7726 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6733 809 Size: 507 5417 1802 Fragments arranged by size: 5417 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4429 5173 6062 Size: 54 1425 50 275 18 2599 744 889 Cuts at: 6062 7691 8 Size: 1629 43 Fragments arranged by size: 2599 1629 1425 889 744 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5173 1537 Size: 275 3361 4090 Fragments arranged by size: 4090 3361 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2360 3219 5656 1353 Size: 1007 859 2437 3423 Fragments arranged by size: 3423 2437 1007 859 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4147 6313 6687 6833 387 Size: 1064 138 2558 2166 374 146 1280 Fragments arranged by size: 2558 2166 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2303 2579 3050 3171 3570 3992 4193 Size: 393 671 276 471 121 399 422 201 Cuts at: 4193 4361 4391 4424 4577 4646 4772 5789 6386 Size: 168 30 33 153 69 126 1017 597 Cuts at: 6386 7173 7441 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1017 787 671 597 471 422 399 393 276 268 201 168 153 126 121 69 33 30 EcoNI CCTnn'n_nnAGG Cuts at: 3270 3270 Size: 7726 EcoO109I rG'GnC_Cy Cuts at: 2379 4012 2379 Size: 1633 6093 EcoRI G'AATT_C Cuts at: 5035 5413 5035 Size: 378 7348 EcoRV GAT'ATC Cuts at: 2536 2536 Size: 7726 FspI TGC'GCA Cuts at: 6232 6232 Size: 7726 HaeII r_GCGC'y Cuts at: 1678 2368 3106 4479 6873 1678 Size: 690 738 1373 2394 2531 Fragments arranged by size: 2531 2394 1373 738 690 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2552 2584 2617 2649 3422 Size: 32 135 32 980 32 33 32 773 Cuts at: 3422 3454 3525 3557 4430 4462 4804 4836 6367 Size: 32 71 32 873 32 342 32 1531 Cuts at: 6367 6399 6441 6473 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1531 980 873 773 342 135 71 42 33 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2847 3147 5070 5316 669 Size: 988 281 909 300 1923 246 3079 Fragments arranged by size: 3079 1923 988 909 300 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7726 HpaI GTT'AAC Cuts at: 2847 5316 2847 Size: 2469 5257 KasI G'GCGC_C Cuts at: 4475 4475 Size: 7726 KpnI G_GTAC'C Cuts at: 5051 5051 Size: 7726 MfeI C'AATT_G Cuts at: 2835 4701 5325 2835 Size: 1866 624 5236 Fragments arranged by size: 5236 1866 624 MluI A'CGCG_T Cuts at: 7602 7602 Size: 7726 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2997 3093 6840 7024 1305 Size: 1692 96 3747 184 2007 Fragments arranged by size: 3747 2007 1692 184 96 MscI TGG'CCA Cuts at: 10 64 1489 1832 4431 10 Size: 54 1425 343 2599 3305 Fragments arranged by size: 3305 2599 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4550 4641 5684 6043 6202 518 Size: 648 3384 91 1043 359 159 2042 Fragments arranged by size: 3384 2042 1043 648 359 159 91 MspA1I CmG'CkG Cuts at: 1606 2375 2893 4235 5142 5819 6967 7212 1606 Size: 769 518 1342 907 677 1148 245 2120 Fragments arranged by size: 2120 1342 1148 907 769 677 518 245 NarI GG'CG_CC Cuts at: 4476 4476 Size: 7726 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6235 Fragments arranged by size: 6235 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7726 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7726 NotI GC'GGCC_GC Cuts at: 5173 5173 Size: 7726 NsiI A_TGCA'T Cuts at: 2343 2343 Size: 7726 NspI r_CATG'y Cuts at: 1132 1229 3319 4076 4117 5084 6629 1132 Size: 97 2090 757 41 967 1545 2229 Fragments arranged by size: 2229 2090 1545 967 757 97 41 PasI CC'CwG_GG Cuts at: 1348 4981 1348 Size: 3633 4093 PciI A'CATG_T Cuts at: 6625 6625 Size: 7726 PflMI CCAn_nnn'nTGG Cuts at: 1108 2326 2390 1108 Size: 1218 64 6444 Fragments arranged by size: 6444 1218 64 PfoI T'CCnGG_A Cuts at: 4008 4368 4008 Size: 360 7366 PmeI GTTT'AAAC Cuts at: 5030 5030 Size: 7726 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5795 5827 7308 7340 5795 Size: 32 1481 32 6181 Fragments arranged by size: 6181 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2379 4012 2379 Size: 1633 6093 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7726 PsiI TTA'TAA Cuts at: 2786 5296 2786 Size: 2510 5216 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3330 3362 3330 Size: 32 7694 PstI C_TGCA'G Cuts at: 830 5078 830 Size: 4248 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7726 PvuII CAG'CTG Cuts at: 2375 4235 2375 Size: 1860 5866 SalI G'TCGA_C Cuts at: 1936 5068 1936 Size: 3132 4594 SanDI GG'GwC_CC Cuts at: 2379 2379 Size: 7726 SapI GCTCTTCn'nnn_ Cuts at: 3219 3219 Size: 7726 SbfI CC_TGCA'GG Cuts at: 5078 5078 Size: 7726 ScaI AGT'ACT Cuts at: 5974 7686 5974 Size: 1712 6014 SfcI C'TryA_G Cuts at: 826 1045 2715 5074 6209 6890 7081 826 Size: 219 1670 2359 1135 681 191 1471 Fragments arranged by size: 2359 1670 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 4477 4477 Size: 7726 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7726 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7726 SmaI CCC'GGG Cuts at: 1872 4589 5053 1872 Size: 2717 464 4545 Fragments arranged by size: 4545 2717 464 SmlI C'TyrA_G Cuts at: 820 1017 1943 5830 6731 6993 820 Size: 197 926 3887 901 262 1553 Fragments arranged by size: 3887 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3472 5517 493 Size: 2979 2045 2702 Fragments arranged by size: 2979 2702 2045 SpeI A'CTAG_T Cuts at: 152 152 Size: 7726 SphI G_CATG'C Cuts at: 1132 5084 1132 Size: 3952 3774 SspI AAT'ATT Cuts at: 5 52 5650 5 Size: 47 5598 2081 Fragments arranged by size: 5598 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7726 StyI C'CwwG_G Cuts at: 513 1065 2004 2384 3205 5165 513 Size: 552 939 380 821 1960 3074 Fragments arranged by size: 3074 1960 939 821 552 380 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3051 3243 5025 5753 5770 5923 6108 Size: 1255 1471 192 1782 728 17 153 185 Cuts at: 6108 6553 325 Size: 445 1498 Fragments arranged by size: 1782 1498 1471 1255 728 445 192 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3853 4443 5972 Size: 274 80 33 51 1443 1876 590 1529 Cuts at: 5972 7684 96 Size: 1712 138 Fragments arranged by size: 1876 1712 1529 1443 590 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2380 2412 3026 3058 4350 4382 4550 4582 7686 Size: 32 614 32 1292 32 168 32 3104 Cuts at: 7686 7718 2380 Size: 32 2388 Fragments arranged by size: 3104 2388 1292 614 168 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2564 1316 Size: 1248 6478 XbaI T'CTAG_A Cuts at: 5062 5062 Size: 7726 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3424 3424 Size: 7726 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7726 XmaI C'CCGG_G Cuts at: 1870 4587 5051 1870 Size: 2717 464 4545 Fragments arranged by size: 4545 2717 464 XmnI GAAnn'nnTTC Cuts at: 2920 5855 2920 Size: 2935 4791 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3807 5534 276 Size: 53 83 186 936 2273 1727 2468 Fragments arranged by size: 2468 2273 1727 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacI SacII SexAI SfiI SrfI SwaI