(Linear) (Six-base) MAPSORT of: KIBB4222.seq Check: 3863 from: 1 to: 3021 KIBB4222.orf With 182 enzymes: SgfI * January 9, 2009 11:10 .. AatII G_ACGT'C Cuts at: 0 1804 3021 Size: 1804 1217 AccI GT'mk_AC Cuts at: 0 449 1743 1751 1804 3021 Size: 449 1294 8 53 1217 Fragments arranged by size: 1294 1217 449 53 8 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 574 2356 2419 3021 Size: 574 1782 63 602 Fragments arranged by size: 1782 602 574 63 AfeI AGC'GCT Cuts at: 0 361 3021 Size: 361 2660 AflIII A'CryG_T Cuts at: 0 1329 1612 2637 3021 Size: 1329 283 1025 384 Fragments arranged by size: 1329 1025 384 283 ApaLI G'TGCA_C Cuts at: 0 2652 3021 Size: 2652 369 ApoI r'AATT_y Cuts at: 0 499 1156 1460 3021 Size: 499 657 304 1561 Fragments arranged by size: 1561 657 499 304 AvaI C'yCGr_G Cuts at: 0 2582 3021 Size: 2582 439 BamHI G'GATC_C Cuts at: 0 307 939 2360 3021 Size: 307 632 1421 661 Fragments arranged by size: 1421 661 632 307 BanI G'GyrC_C Cuts at: 0 2470 2904 3010 3021 Size: 2470 434 106 11 Fragments arranged by size: 2470 434 106 11 BanII G_rGCy'C Cuts at: 0 2984 3021 Size: 2984 37 BbeI G_GCGC'C Cuts at: 0 2474 3021 Size: 2474 547 BciVI GTATCCnnnnn_n' Cuts at: 0 2518 3021 Size: 2518 503 BclI T'GATC_A Cuts at: 0 2132 3021 Size: 2132 889 BfrBI ATG'CAT Cuts at: 0 336 3021 Size: 336 2685 BglII A'GATC_T Cuts at: 0 167 3021 Size: 167 2854 Bme1580I G_kGCm'C Cuts at: 0 2656 2907 3021 Size: 2656 251 114 Fragments arranged by size: 2656 251 114 BmgBI CAC'GTC Cuts at: 0 299 2558 2638 3021 Size: 299 2259 80 383 Fragments arranged by size: 2259 383 299 80 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 298 1045 1166 1565 1987 2188 2386 2572 Size: 298 747 121 399 422 201 198 186 Cuts at: 2572 2641 2767 3021 Size: 69 126 254 Fragments arranged by size: 747 422 399 298 254 201 198 186 126 121 69 Bpu10I CC'TnA_GC Cuts at: 0 2873 3021 Size: 2873 148 BsaI GGTCTCn'nnnn_ Cuts at: 0 47 1105 1371 3021 Size: 47 1058 266 1650 Fragments arranged by size: 1650 1058 266 47 BsaAI yAC'GTr Cuts at: 0 1330 1467 3021 Size: 1330 137 1554 Fragments arranged by size: 1554 1330 137 BsaBI GATnn'nnATC Cuts at: 0 1930 3021 Size: 1930 1091 BsaHI Gr'CG_yC Cuts at: 0 329 1801 2471 3021 Size: 329 1472 670 550 Fragments arranged by size: 1472 670 550 329 BsaWI w'CCGG_w Cuts at: 0 1473 3021 Size: 1473 1548 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 375 405 1520 1550 1563 1593 2545 2575 Size: 375 30 1115 30 13 30 952 30 Cuts at: 2575 3021 Size: 446 Fragments arranged by size: 1115 952 446 375 30 30 30 30 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 178 3021 Size: 178 2843 BseYI C'CCAG_C Cuts at: 0 370 3021 Size: 370 2651 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 279 3021 Size: 279 2742 BsiHKAI G_wGCw'C Cuts at: 0 2627 2656 2879 3021 Size: 2627 29 223 142 Fragments arranged by size: 2627 223 142 29 BsmI GAATG_Cn' Cuts at: 0 69 2183 3021 Size: 69 2114 838 Fragments arranged by size: 2114 838 69 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2548 3021 Size: 2548 473 Bsp1286I G_dGCh'C Cuts at: 0 2627 2656 2879 2907 2984 3021 Size: 2627 29 223 28 77 37 Fragments arranged by size: 2627 223 77 37 29 28 BsrDI GCAATG_nn' Cuts at: 0 1715 2151 3021 Size: 1715 436 870 Fragments arranged by size: 1715 870 436 BsrGI T'GTAC_A Cuts at: 0 1848 3021 Size: 1848 1173 BstBI TT'CG_AA Cuts at: 0 1239 1647 3021 Size: 1239 408 1374 Fragments arranged by size: 1374 1239 408 BstEII G'GTnAC_C Cuts at: 0 145 3021 Size: 145 2876 BstYI r'GATC_y Cuts at: 0 167 307 939 1840 2360 2807 3021 Size: 167 140 632 901 520 447 214 Fragments arranged by size: 901 632 520 447 214 167 140 BtgI C'CryG_G Cuts at: 0 534 897 3021 Size: 534 363 2124 Fragments arranged by size: 2124 534 363 BtsI GCAGTG_nn' Cuts at: 0 134 3021 Size: 134 2887 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 615 650 1107 1142 3021 Size: 615 35 457 35 1879 Fragments arranged by size: 1879 615 457 35 35 EaeI y'GGCC_r Cuts at: 0 2424 3021 Size: 2424 597 EarI CTCTTCn'nnn_ Cuts at: 0 355 1214 3021 Size: 355 859 1807 Fragments arranged by size: 1807 859 355 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2142 3021 Size: 2142 879 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 298 574 1045 1166 1565 1987 2188 2356 Size: 298 276 471 121 399 422 201 168 Cuts at: 2356 2386 2419 2572 2641 2767 3021 Size: 30 33 153 69 126 254 Fragments arranged by size: 471 422 399 298 276 254 201 168 153 126 121 69 33 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 1265 3021 Size: 1265 1756 EcoO109I rG'GnC_Cy Cuts at: 0 374 2007 3021 Size: 374 1633 1014 Fragments arranged by size: 1633 1014 374 EcoRV GAT'ATC Cuts at: 0 531 3021 Size: 531 2490 HaeII r_GCGC'y Cuts at: 0 363 1101 2474 3021 Size: 363 738 1373 547 Fragments arranged by size: 1373 738 547 363 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 547 579 612 644 1417 1449 1520 1552 Size: 547 32 33 32 773 32 71 32 Cuts at: 1552 2425 2457 2799 2831 3021 Size: 873 32 342 32 190 Fragments arranged by size: 873 773 547 342 190 71 33 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 842 1142 3021 Size: 842 300 1879 Fragments arranged by size: 1879 842 300 HpaI GTT'AAC Cuts at: 0 842 3021 Size: 842 2179 KasI G'GCGC_C Cuts at: 0 2470 3021 Size: 2470 551 MfeI C'AATT_G Cuts at: 0 830 2696 3021 Size: 830 1866 325 Fragments arranged by size: 1866 830 325 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 992 1088 3021 Size: 992 96 1933 Fragments arranged by size: 1933 992 96 MscI TGG'CCA Cuts at: 0 2426 3021 Size: 2426 595 MslI CAynn'nnrTG Cuts at: 0 2545 2636 3021 Size: 2545 91 385 Fragments arranged by size: 2545 385 91 MspA1I CmG'CkG Cuts at: 0 370 888 2230 3021 Size: 370 518 1342 791 Fragments arranged by size: 1342 791 518 370 NarI GG'CG_CC Cuts at: 0 2471 3021 Size: 2471 550 NsiI A_TGCA'T Cuts at: 0 338 3021 Size: 338 2683 NspI r_CATG'y Cuts at: 0 1314 2071 2112 3021 Size: 1314 757 41 909 Fragments arranged by size: 1314 909 757 41 PasI CC'CwG_GG Cuts at: 0 2976 3021 Size: 2976 45 PflMI CCAn_nnn'nTGG Cuts at: 0 321 385 3021 Size: 321 64 2636 Fragments arranged by size: 2636 321 64 PfoI T'CCnGG_A Cuts at: 0 2003 2363 3021 Size: 2003 360 658 Fragments arranged by size: 2003 658 360 PpuMI rG'GwC_Cy Cuts at: 0 374 2007 3021 Size: 374 1633 1014 Fragments arranged by size: 1633 1014 374 PsiI TTA'TAA Cuts at: 0 781 3021 Size: 781 2240 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1325 1357 3021 Size: 1325 32 1664 Fragments arranged by size: 1664 1325 32 PvuII CAG'CTG Cuts at: 0 370 2230 3021 Size: 370 1860 791 Fragments arranged by size: 1860 791 370 SanDI GG'GwC_CC Cuts at: 0 374 3021 Size: 374 2647 SapI GCTCTTCn'nnn_ Cuts at: 0 1214 3021 Size: 1214 1807 SfcI C'TryA_G Cuts at: 0 710 3021 Size: 710 2311 SfoI GGC'GCC Cuts at: 0 2472 3021 Size: 2472 549 SmaI CCC'GGG Cuts at: 0 2584 3021 Size: 2584 437 SnaBI TAC'GTA Cuts at: 0 1467 3021 Size: 1467 1554 StyI C'CwwG_G Cuts at: 0 379 1200 3021 Size: 379 821 1821 Fragments arranged by size: 1821 821 379 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1046 1238 3020 3021 Size: 1046 192 1782 1 Fragments arranged by size: 1782 1046 192 1 TatI w'GTAC_w Cuts at: 0 1848 2438 3021 Size: 1848 590 583 Fragments arranged by size: 1848 590 583 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 375 407 1021 1053 2345 2377 2545 2577 Size: 375 32 614 32 1292 32 168 32 Cuts at: 2577 3021 Size: 444 Fragments arranged by size: 1292 614 444 375 168 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 559 3021 Size: 559 2462 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1419 3021 Size: 1419 1602 XmaI C'CCGG_G Cuts at: 0 2582 3021 Size: 2582 439 XmnI GAAnn'nnTTC Cuts at: 0 915 3021 Size: 915 2106 ZraI GAC'GTC Cuts at: 0 1802 3021 Size: 1802 1219 Enzymes that do cut: AatII AccI AcuI AfeI AflIII ApaLI ApoI AvaI BamHI BanI BanII BbeI BciVI BclI BfrBI BglII Bme1580I BmgBI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BsrDI BsrGI BstBI BstEII BstYI BtgI BtsI CspCI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII HpaI KasI MfeI MmeI MscI MslI MspA1I NarI NsiI NspI PasI PflMI PfoI PpuMI PsiI PsrI PvuII SanDI SapI SfcI SfoI SmaI SnaBI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AflII AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI BbsI BbvCI BcgI BglI BlpI BmrI BmtI BplI BpuEI BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstXI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EcoICRI EcoRI FalI FseI FspI FspAI HindIII KpnI MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI PacI PciI PmeI PmlI PpiI PshAI PspOMI PspXI PstI PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SpeI SphI SrfI SspI StuI SwaI XbaI XhoI