(Circular) (Six-base) MAPSORT of: pFN21AB8824.seq Check: 6091 from: 1 to: 5947 pFN21AB8824 5947 bp With 182 enzymes: SgfI * February 27, 2009 14:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3757 278 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3290 1937 Size: 1353 4594 Acc65I G'GTAC_C Cuts at: 3268 3268 Size: 5947 AclI AA'CG_TT Cuts at: 1563 4074 4447 1563 Size: 2511 373 3063 Fragments arranged by size: 3063 2511 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2299 2966 4010 5394 5662 1632 Size: 667 667 1044 1384 268 1917 Fragments arranged by size: 1917 1384 1044 667 667 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5750 AflIII A'CryG_T Cuts at: 1203 2287 4846 5823 1203 Size: 1084 2559 977 1327 Fragments arranged by size: 2559 1327 1084 977 AgeI A'CCGG_T Cuts at: 4778 4778 Size: 5947 AhdI GACnn_n'nnGTC Cuts at: 4676 4676 Size: 5947 AleI CACnn'nnGTG Cuts at: 1166 2024 2237 1166 Size: 858 213 4876 Fragments arranged by size: 4876 858 213 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2318 1184 Size: 1134 4813 AlwNI CAG_nnn'CTG Cuts at: 2848 2944 3122 3690 5262 2848 Size: 96 178 568 1572 3533 Fragments arranged by size: 3533 1572 568 178 96 ApaI G_GGCC'C Cuts at: 2884 2884 Size: 5947 ApaLI G'TGCA_C Cuts at: 4004 5160 4004 Size: 1156 4791 ApoI r'AATT_y Cuts at: 1493 3256 3482 3634 5743 1493 Size: 1763 226 152 2109 1697 Fragments arranged by size: 2109 1763 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4501 160 Size: 872 3469 1606 Fragments arranged by size: 3469 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 AvaI C'yCGr_G Cuts at: 1870 1943 3272 1870 Size: 73 1329 4545 Fragments arranged by size: 4545 1329 73 AvrII C'CTAG_G Cuts at: 2218 2218 Size: 5947 BamHI G'GATC_C Cuts at: 3277 3644 3277 Size: 367 5580 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2185 2384 2499 2812 2821 Size: 325 217 627 398 199 115 313 9 Cuts at: 2821 3268 4723 618 Size: 447 1455 1842 Fragments arranged by size: 1842 1455 627 447 398 325 313 217 199 115 9 BanII G_rGCy'C Cuts at: 1391 2353 2478 2600 2884 3147 3266 1391 Size: 962 125 122 284 263 119 4072 Fragments arranged by size: 4072 962 284 263 125 122 119 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2503 2747 928 Size: 971 604 244 4128 Fragments arranged by size: 4128 971 604 244 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4138 4172 1903 Size: 34 50 34 2117 34 3678 Fragments arranged by size: 3678 2117 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3839 5049 3839 Size: 1210 4737 BclI T'GATC_A Cuts at: 1548 1548 Size: 5947 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2138 4558 136 Size: 107 122 71 631 605 466 2420 1525 Fragments arranged by size: 2420 1525 631 605 466 122 107 71 BglII A'GATC_T Cuts at: 5942 5942 Size: 5947 BlpI GC'TnA_GC Cuts at: 3364 3364 Size: 5947 Bme1580I G_kGCm'C Cuts at: 1790 2502 2634 2817 2826 2884 4008 5164 1790 Size: 712 132 183 9 58 1124 1156 2573 Fragments arranged by size: 2573 1156 1124 712 183 132 58 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5947 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2858 3678 4636 Size: 416 53 83 399 24 1441 820 958 Cuts at: 4636 442 Size: 1753 Fragments arranged by size: 1753 1441 958 820 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5947 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3148 3148 Size: 5947 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 3064 4607 1239 Size: 797 1028 1543 2579 Fragments arranged by size: 2579 1543 1028 797 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2606 2638 4072 4937 5235 2606 Size: 32 1434 865 298 3318 Fragments arranged by size: 3318 1434 865 298 32 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4610 882 Size: 617 3111 2219 Fragments arranged by size: 3111 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2288 3738 493 Size: 711 1084 1450 2702 Fragments arranged by size: 2702 1450 1084 711 BsaBI GATnn'nnATC Cuts at: 5941 5941 Size: 5947 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3754 4136 4771 275 Size: 53 83 186 936 2221 382 635 1451 Fragments arranged by size: 2221 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4380 4778 5052 5199 5610 4380 Size: 398 274 147 411 4717 Fragments arranged by size: 4717 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3037 3067 1178 Size: 30 165 30 1634 30 4058 Fragments arranged by size: 4058 1634 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5947 BseYI C'CCAG_C Cuts at: 3012 3076 3103 3233 5150 5646 3012 Size: 64 27 130 1917 496 3313 Fragments arranged by size: 3313 1917 496 130 64 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5374 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3397 4158 5186 1540 Size: 275 186 1396 761 1028 2301 Fragments arranged by size: 2301 1396 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2031 3147 4008 4093 5164 2031 Size: 1116 861 85 1071 2814 Fragments arranged by size: 2814 1116 1071 861 85 BsmI GAATG_Cn' Cuts at: 3458 3551 3458 Size: 93 5854 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2353 2478 2502 2600 2634 2817 Size: 399 241 322 125 24 98 34 183 Cuts at: 2817 2826 2884 3147 3266 4008 4093 5164 1391 Size: 9 58 263 119 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 322 263 241 183 125 119 98 85 58 34 24 9 BspEI T'CCGG_A Cuts at: 5610 5610 Size: 5947 BspHI T'CATG_A Cuts at: 3836 5566 3836 Size: 1730 4217 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2598 3171 3302 844 Size: 1754 573 131 3489 Fragments arranged by size: 3489 1754 573 131 BsrBI CCG'CTC Cuts at: 1394 1823 3834 1394 Size: 429 2011 3507 Fragments arranged by size: 3507 2011 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4442 4616 65 Size: 1183 3194 174 1396 Fragments arranged by size: 3194 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2802 4591 4778 1700 Size: 110 992 1789 187 2869 Fragments arranged by size: 2869 1789 992 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5947 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5947 BssSI C'ACGA_G Cuts at: 4007 5019 4007 Size: 1012 4935 BstAPI GCAn_nnn'nTGC Cuts at: 794 3055 794 Size: 2261 3686 BstBI TT'CG_AA Cuts at: 4751 4751 Size: 5947 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5947 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5947 BstYI r'GATC_y Cuts at: 1972 3118 3277 3644 4029 4046 5498 5584 5596 Size: 1146 159 367 385 17 1452 86 12 Cuts at: 5596 5942 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1146 385 367 346 159 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2703 2703 Size: 5947 BtgI C'CryG_G Cuts at: 513 1065 2854 2983 513 Size: 552 1789 129 3477 Fragments arranged by size: 3477 1789 552 129 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5208 Fragments arranged by size: 5208 683 56 BtsI GCAGTG_nn' Cuts at: 2167 2573 2904 3463 4257 4277 2167 Size: 406 331 559 794 20 3837 Fragments arranged by size: 3837 794 559 406 331 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5947 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5912 DraI TTT'AAA Cuts at: 3251 3251 Size: 5947 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4954 809 Size: 507 3638 1802 Fragments arranged by size: 3638 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2139 2448 2769 Size: 54 1425 50 275 18 309 309 321 Cuts at: 2769 2885 3394 4283 5912 8 Size: 116 509 889 1629 43 Fragments arranged by size: 1629 1425 889 509 321 309 309 275 116 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3394 1537 Size: 275 1582 4090 Fragments arranged by size: 4090 1582 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2175 3877 1353 Size: 822 1702 3423 Fragments arranged by size: 3423 1702 822 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4534 4908 5054 387 Size: 1064 138 2945 374 146 1280 Fragments arranged by size: 2945 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3145 3145 Size: 5947 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2299 2966 3064 4010 4607 5394 Size: 393 404 263 667 98 946 597 787 Cuts at: 5394 5662 1239 Size: 268 1524 Fragments arranged by size: 1524 946 787 667 597 404 393 268 263 98 EcoO109I rG'GnC_Cy Cuts at: 2094 2880 2094 Size: 786 5161 EcoRI G'AATT_C Cuts at: 3256 3634 3256 Size: 378 5569 FspI TGC'GCA Cuts at: 4453 4453 Size: 5947 HaeII r_GCGC'y Cuts at: 1678 3100 5094 1678 Size: 1422 1994 2531 Fragments arranged by size: 2531 1994 1422 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2627 2659 2850 2882 4588 Size: 32 135 32 1055 32 191 32 1706 Cuts at: 4588 4620 4662 4694 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1706 1055 191 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2956 3291 3537 669 Size: 988 281 1018 335 246 3079 Fragments arranged by size: 3079 1018 988 335 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5947 HpaI GTT'AAC Cuts at: 3537 3537 Size: 5947 KpnI G_GTAC'C Cuts at: 3272 3272 Size: 5947 MfeI C'AATT_G Cuts at: 3546 3546 Size: 5947 MluI A'CGCG_T Cuts at: 5823 5823 Size: 5947 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5061 5245 1305 Size: 3756 184 2007 Fragments arranged by size: 3756 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2450 2771 10 Size: 54 1425 343 618 321 3186 Fragments arranged by size: 3186 1425 618 343 321 54 MslI CAynn'nnrTG Cuts at: 518 1166 2024 2237 2324 2505 2969 3905 4264 Size: 648 858 213 87 181 464 936 359 Cuts at: 4264 4423 518 Size: 159 2042 Fragments arranged by size: 2042 936 858 648 464 359 213 181 159 87 MspA1I CmG'CkG Cuts at: 1606 2192 2547 3016 3363 4040 5188 5433 1606 Size: 586 355 469 347 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 586 469 355 347 245 NaeI GCC'GGC Cuts at: 2804 2804 Size: 5947 NcoI C'CATG_G Cuts at: 513 1065 2854 513 Size: 552 1789 3606 Fragments arranged by size: 3606 1789 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5947 NgoMIV G'CCGG_C Cuts at: 2802 2802 Size: 5947 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5947 NotI GC'GGCC_GC Cuts at: 3394 3394 Size: 5947 NspI r_CATG'y Cuts at: 1132 1229 2305 2329 2897 3305 4850 1132 Size: 97 1076 24 568 408 1545 2229 Fragments arranged by size: 2229 1545 1076 568 408 97 24 PasI CC'CwG_GG Cuts at: 1348 2808 1348 Size: 1460 4487 PciI A'CATG_T Cuts at: 4846 4846 Size: 5947 PflMI CCAn_nnn'nTGG Cuts at: 1108 2523 1108 Size: 1415 4532 PfoI T'CCnGG_A Cuts at: 2457 2754 2457 Size: 297 5650 PmeI GTTT'AAAC Cuts at: 3251 3251 Size: 5947 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4016 4048 5529 5561 4016 Size: 32 1481 32 4402 Fragments arranged by size: 4402 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2094 2094 Size: 5947 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5947 PsiI TTA'TAA Cuts at: 3517 3517 Size: 5947 PspOMI G'GGCC_C Cuts at: 2880 2880 Size: 5947 PstI C_TGCA'G Cuts at: 830 2595 3299 830 Size: 1765 704 3478 Fragments arranged by size: 3478 1765 704 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5947 PvuII CAG'CTG Cuts at: 2192 3016 2192 Size: 824 5123 SacI G_AGCT'C Cuts at: 3147 3147 Size: 5947 SalI G'TCGA_C Cuts at: 1936 3289 1936 Size: 1353 4594 SbfI CC_TGCA'GG Cuts at: 3299 3299 Size: 5947 ScaI AGT'ACT Cuts at: 4195 5907 4195 Size: 1712 4235 SfcI C'TryA_G Cuts at: 826 1045 2193 2591 3295 4430 5111 5302 826 Size: 219 1148 398 704 1135 681 191 1471 Fragments arranged by size: 1471 1148 1135 704 681 398 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5947 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5947 SmaI CCC'GGG Cuts at: 1872 3274 1872 Size: 1402 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2621 2653 4051 4952 5214 820 Size: 197 926 678 32 1398 901 262 1553 Fragments arranged by size: 1553 1398 926 901 678 262 197 32 SnaBI TAC'GTA Cuts at: 493 3738 493 Size: 3245 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5947 SphI G_CATG'C Cuts at: 1132 2897 3305 1132 Size: 1765 408 3774 Fragments arranged by size: 3774 1765 408 SspI AAT'ATT Cuts at: 5 52 3871 5 Size: 47 3819 2081 Fragments arranged by size: 3819 2081 47 StuI AGG'CCT Cuts at: 1517 2057 2370 1517 Size: 540 313 5094 Fragments arranged by size: 5094 540 313 StyI C'CwwG_G Cuts at: 513 1065 2218 2666 2682 2854 3386 513 Size: 552 1153 448 16 172 532 3074 Fragments arranged by size: 3074 1153 552 532 448 172 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2800 3026 3068 3974 3991 4144 4329 Size: 1255 1220 226 42 906 17 153 185 Cuts at: 4329 4774 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1220 906 445 226 185 153 42 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2373 4193 5905 Size: 274 80 33 51 1443 396 1820 1712 Cuts at: 5905 96 Size: 138 Fragments arranged by size: 1820 1712 1443 396 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2524 2556 3037 3069 5907 5939 2524 Size: 32 481 32 2838 32 2532 Fragments arranged by size: 2838 2532 481 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5947 XbaI T'CTAG_A Cuts at: 3283 3283 Size: 5947 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2196 2196 Size: 5947 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5947 XmaI C'CCGG_G Cuts at: 1870 3272 1870 Size: 1402 4545 XmnI GAAnn'nnTTC Cuts at: 4076 4076 Size: 5947 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3755 276 Size: 53 83 186 936 2221 2468 Fragments arranged by size: 2468 2221 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI