(Circular) (Six-base) MAPSORT of: pFN21AB3126.seq Check: 1798 from: 1 to: 7609 pFN21AB3126 7609 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2334 2334 Size: 7609 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4035 5419 278 Size: 53 83 186 936 2499 1384 2468 Fragments arranged by size: 2499 2468 1384 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2425 4952 1937 Size: 488 2527 4594 Fragments arranged by size: 4594 2527 488 Acc65I G'GTAC_C Cuts at: 4930 4930 Size: 7609 AclI AA'CG_TT Cuts at: 1563 5736 6109 1563 Size: 4173 373 3063 Fragments arranged by size: 4173 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3354 4461 5672 7056 7324 1632 Size: 1722 1107 1211 1384 268 1917 Fragments arranged by size: 1917 1722 1384 1211 1107 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7412 AflIII A'CryG_T Cuts at: 1203 6508 7485 1203 Size: 5305 977 1327 Fragments arranged by size: 5305 1327 977 AgeI A'CCGG_T Cuts at: 6440 6440 Size: 7609 AhdI GACnn_n'nnGTC Cuts at: 6338 6338 Size: 7609 AleI CACnn'nnGTG Cuts at: 1166 3614 1166 Size: 2448 5161 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3561 3885 4010 4085 1184 Size: 2377 324 125 75 4708 Fragments arranged by size: 4708 2377 324 125 75 AlwNI CAG_nnn'CTG Cuts at: 2987 4776 5352 6924 2987 Size: 1789 576 1572 3672 Fragments arranged by size: 3672 1789 1572 576 ApaLI G'TGCA_C Cuts at: 2516 5666 6822 2516 Size: 3150 1156 3303 Fragments arranged by size: 3303 3150 1156 ApoI r'AATT_y Cuts at: 1493 2805 4476 4918 5144 5296 7405 1493 Size: 1312 1671 442 226 152 2109 1697 Fragments arranged by size: 2109 1697 1671 1312 442 226 152 AseI AT'TA_AT Cuts at: 160 1032 2967 6163 160 Size: 872 1935 3196 1606 Fragments arranged by size: 3196 1935 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7609 AvaI C'yCGr_G Cuts at: 1870 1943 4934 1870 Size: 73 2991 4545 Fragments arranged by size: 4545 2991 73 BamHI G'GATC_C Cuts at: 2056 4939 5306 2056 Size: 2883 367 4359 Fragments arranged by size: 4359 2883 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2216 2691 3140 4838 4930 Size: 325 217 627 429 475 449 1698 92 Cuts at: 4930 6385 618 Size: 1455 1842 Fragments arranged by size: 1842 1698 1455 627 475 449 429 325 217 92 BanII G_rGCy'C Cuts at: 1391 3417 4758 4928 1391 Size: 2026 1341 170 4072 Fragments arranged by size: 4072 2026 1341 170 BbeI G_GCGC'C Cuts at: 2695 2695 Size: 7609 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2932 4697 928 Size: 971 1033 1765 3840 Fragments arranged by size: 3840 1765 1033 971 BbvCI CC'TCA_GC Cuts at: 2630 3809 2630 Size: 1179 6430 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5800 5834 1903 Size: 34 3863 34 3678 Fragments arranged by size: 3863 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4524 5501 6711 4524 Size: 977 1210 5422 Fragments arranged by size: 5422 1210 977 BclI T'GATC_A Cuts at: 1548 3774 1548 Size: 2226 5383 BfrBI ATG'CAT Cuts at: 2744 2744 Size: 7609 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4025 6220 136 Size: 107 122 71 631 605 2353 2195 1525 Fragments arranged by size: 2353 2195 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7604 7604 Size: 7609 BlpI GC'TnA_GC Cuts at: 5026 5026 Size: 7609 Bme1580I G_kGCm'C Cuts at: 1790 2520 3194 5670 6826 1790 Size: 730 674 2476 1156 2573 Fragments arranged by size: 2573 2476 1156 730 674 BmgBI CAC'GTC Cuts at: 1318 3577 1318 Size: 2259 5350 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4507 4679 5340 Size: 416 53 83 399 24 3090 172 661 Cuts at: 5340 6298 442 Size: 958 1753 Fragments arranged by size: 3090 1753 958 661 416 399 172 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7609 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4578 4578 Size: 7609 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2814 3171 3507 4142 4663 6269 1239 Size: 1575 357 336 635 521 1606 2579 Fragments arranged by size: 2579 1606 1575 635 521 357 336 Bpu10I CC'TnA_GC Cuts at: 2630 3809 3827 4041 2630 Size: 1179 18 214 6198 Fragments arranged by size: 6198 1179 214 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3893 5734 6599 6897 3893 Size: 1841 865 298 4605 Fragments arranged by size: 4605 1841 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4600 6272 882 Size: 617 3101 1672 2219 Fragments arranged by size: 3101 2219 1672 617 BsaAI yAC'GTr Cuts at: 493 1204 5400 493 Size: 711 4196 2702 Fragments arranged by size: 4196 2702 711 BsaBI GATnn'nnATC Cuts at: 7603 7603 Size: 7609 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2692 2715 3755 4032 Size: 53 83 186 936 1159 23 1040 277 Cuts at: 4032 5416 5798 6433 275 Size: 1384 382 635 1451 Fragments arranged by size: 1451 1384 1159 1040 936 635 382 277 186 83 53 23 BsaWI w'CCGG_w Cuts at: 2665 6042 6440 6714 6861 7272 2665 Size: 3377 398 274 147 411 3002 Fragments arranged by size: 3377 3002 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4037 4067 1178 Size: 30 165 30 2634 30 4720 Fragments arranged by size: 4720 2634 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3198 1186 Size: 2012 5597 BseYI C'CCAG_C Cuts at: 2566 3287 3416 3815 4022 4184 6812 7308 2566 Size: 721 129 399 207 162 2628 496 2867 Fragments arranged by size: 2867 2628 721 496 399 207 162 129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2356 3101 3638 4055 1163 Size: 573 620 745 537 417 4717 Fragments arranged by size: 4717 745 620 573 537 417 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5059 5820 6848 1540 Size: 275 186 3058 761 1028 2301 Fragments arranged by size: 3058 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2520 4089 4758 5670 5755 6826 2520 Size: 1569 669 912 85 1071 3303 Fragments arranged by size: 3303 1569 1071 912 669 85 BsmI GAATG_Cn' Cuts at: 3967 5120 5213 3967 Size: 1153 93 6363 Fragments arranged by size: 6363 1153 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3567 3567 Size: 7609 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2520 3194 3417 4089 4758 4928 5670 Size: 399 730 674 223 672 669 170 742 Cuts at: 5670 5755 6826 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 730 674 672 669 399 223 170 85 BspEI T'CCGG_A Cuts at: 2665 7272 2665 Size: 4607 3002 BspHI T'CATG_A Cuts at: 5498 7228 5498 Size: 1730 5879 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2334 4342 4964 844 Size: 1490 2008 622 3489 Fragments arranged by size: 3489 2008 1490 622 BsrBI CCG'CTC Cuts at: 1394 1823 5496 1394 Size: 429 3673 3507 Fragments arranged by size: 3673 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 6104 6278 65 Size: 1183 4856 174 1396 Fragments arranged by size: 4856 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2559 6253 6440 1700 Size: 110 749 3694 187 2869 Fragments arranged by size: 3694 2869 749 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7609 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7609 BssSI C'ACGA_G Cuts at: 5669 6681 5669 Size: 1012 6597 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7609 BstBI TT'CG_AA Cuts at: 6413 6413 Size: 7609 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7609 BstXI CCAn_nnnn'nTGG Cuts at: 2398 2623 4204 4877 2398 Size: 225 1581 673 5130 Fragments arranged by size: 5130 1581 673 225 BstYI r'GATC_y Cuts at: 1972 2056 2367 3685 4234 4939 5306 5691 5708 Size: 84 311 1318 549 705 367 385 17 Cuts at: 5708 7160 7246 7258 7604 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1318 705 549 385 367 346 311 86 84 17 12 BstZ17I GTA'TAC Cuts at: 2426 2426 Size: 7609 Bsu36I CC'TnA_GG Cuts at: 2250 2250 Size: 7609 BtgI C'CryG_G Cuts at: 513 1065 2004 2200 2703 3306 513 Size: 552 939 196 503 603 4816 Fragments arranged by size: 4816 939 603 552 503 196 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6870 Fragments arranged by size: 6870 683 56 BtsI GCAGTG_nn' Cuts at: 2111 3609 3777 4067 4844 5125 5919 5939 2111 Size: 1498 168 290 777 281 794 20 3781 Fragments arranged by size: 3781 1498 794 777 290 281 168 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7609 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7574 DraI TTT'AAA Cuts at: 4913 4913 Size: 7609 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2402 6616 809 Size: 507 1086 4214 1802 Fragments arranged by size: 4214 1802 1086 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3591 4017 4338 Size: 54 1425 50 275 18 1761 426 321 Cuts at: 4338 5056 5945 7574 8 Size: 718 889 1629 43 Fragments arranged by size: 1761 1629 1425 889 718 426 321 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5056 1537 Size: 275 3244 4090 Fragments arranged by size: 4090 3244 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2856 3471 4218 5539 1353 Size: 1503 615 747 1321 3423 Fragments arranged by size: 3423 1503 1321 747 615 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2582 3171 6196 6570 6716 387 Size: 1064 138 993 589 3025 374 146 1280 Fragments arranged by size: 3025 1280 1064 993 589 374 146 138 EcoICRI GAG'CTC Cuts at: 4756 4756 Size: 7609 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2814 3171 3354 3507 4142 4461 4663 Size: 393 1182 357 183 153 635 319 202 Cuts at: 4663 5672 6269 7056 7324 1239 Size: 1009 597 787 268 1524 Fragments arranged by size: 1524 1182 1009 787 635 597 393 357 319 268 202 183 153 EcoNI CCTnn'n_nnAGG Cuts at: 4735 4896 4735 Size: 161 7448 EcoO109I rG'GnC_Cy Cuts at: 3328 4627 4889 3328 Size: 1299 262 6048 Fragments arranged by size: 6048 1299 262 EcoRI G'AATT_C Cuts at: 4918 5296 4918 Size: 378 7231 FspI TGC'GCA Cuts at: 6115 6115 Size: 7609 HaeII r_GCGC'y Cuts at: 1678 2695 3604 6756 1678 Size: 1017 909 3152 2531 Fragments arranged by size: 3152 2531 1017 909 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3076 3108 3302 3334 3727 Size: 32 135 32 1504 32 194 32 393 Cuts at: 3727 3759 4037 4069 4082 4114 4382 4414 4684 Size: 32 278 32 13 32 268 32 270 Cuts at: 4684 4716 6250 6282 6324 6356 1373 Size: 32 1534 32 42 32 2626 Fragments arranged by size: 2626 1534 1504 393 278 270 268 194 135 42 32 32 32 32 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 4953 5199 669 Size: 988 281 3015 246 3079 Fragments arranged by size: 3079 3015 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7609 HpaI GTT'AAC Cuts at: 5199 5199 Size: 7609 KasI G'GCGC_C Cuts at: 2691 2691 Size: 7609 KpnI G_GTAC'C Cuts at: 4934 4934 Size: 7609 MfeI C'AATT_G Cuts at: 4656 5208 4656 Size: 552 7057 MluI A'CGCG_T Cuts at: 7485 7485 Size: 7609 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2772 6723 6907 1305 Size: 1467 3951 184 2007 Fragments arranged by size: 3951 2007 1467 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4340 10 Size: 54 1425 343 2508 3279 Fragments arranged by size: 3279 2508 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2328 3543 3614 4875 5567 5926 6085 Size: 648 1162 1215 71 1261 692 359 159 Cuts at: 6085 518 Size: 2042 Fragments arranged by size: 2042 1261 1215 1162 692 648 359 159 71 MspA1I CmG'CkG Cuts at: 1606 2566 2780 4022 4184 4347 4435 4459 4785 Size: 960 214 1242 162 163 88 24 326 Cuts at: 4785 5025 5702 6850 7095 1606 Size: 240 677 1148 245 2120 Fragments arranged by size: 2120 1242 1148 960 677 326 245 240 214 163 162 88 24 NarI GG'CG_CC Cuts at: 2692 2692 Size: 7609 NcoI C'CATG_G Cuts at: 513 1065 2004 2200 3306 513 Size: 552 939 196 1106 4816 Fragments arranged by size: 4816 1106 939 552 196 NdeI CA'TA_TG Cuts at: 387 4381 387 Size: 3994 3615 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7609 NotI GC'GGCC_GC Cuts at: 5056 5056 Size: 7609 NsiI A_TGCA'T Cuts at: 2746 2746 Size: 7609 NspI r_CATG'y Cuts at: 1132 1229 2333 3166 3542 4967 6512 1132 Size: 97 1104 833 376 1425 1545 2229 Fragments arranged by size: 2229 1545 1425 1104 833 376 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7609 PciI A'CATG_T Cuts at: 6508 6508 Size: 7609 PflMI CCAn_nnn'nTGG Cuts at: 1108 2123 2201 2341 3230 4347 1108 Size: 1015 78 140 889 1117 4370 Fragments arranged by size: 4370 1117 1015 889 140 78 PfoI T'CCnGG_A Cuts at: 2503 3265 4119 2503 Size: 762 854 5993 Fragments arranged by size: 5993 854 762 PmeI GTTT'AAAC Cuts at: 4913 4913 Size: 7609 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4312 4344 5678 5710 7191 7223 4312 Size: 32 1334 32 1481 32 4698 Fragments arranged by size: 4698 1481 1334 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3328 4889 3328 Size: 1561 6048 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7609 PsiI TTA'TAA Cuts at: 5179 5179 Size: 7609 PstI C_TGCA'G Cuts at: 830 2610 4458 4961 830 Size: 1780 1848 503 3478 Fragments arranged by size: 3478 1848 1780 503 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7609 PvuII CAG'CTG Cuts at: 2566 2780 4184 4347 4435 4459 2566 Size: 214 1404 163 88 24 5716 Fragments arranged by size: 5716 1404 214 163 88 24 SacI G_AGCT'C Cuts at: 4758 4758 Size: 7609 SalI G'TCGA_C Cuts at: 1936 4951 1936 Size: 3015 4594 SapI GCTCTTCn'nnn_ Cuts at: 4218 4218 Size: 7609 SbfI CC_TGCA'GG Cuts at: 4961 4961 Size: 7609 ScaI AGT'ACT Cuts at: 4147 5857 7569 4147 Size: 1710 1712 4187 Fragments arranged by size: 4187 1712 1710 SexAI A'CCwGG_T Cuts at: 2212 2212 Size: 7609 SfcI C'TryA_G Cuts at: 826 1045 2606 4454 4957 6092 6773 6964 826 Size: 219 1561 1848 503 1135 681 191 1471 Fragments arranged by size: 1848 1561 1471 1135 681 503 219 191 SfoI GGC'GCC Cuts at: 2693 2693 Size: 7609 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7609 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7609 SmaI CCC'GGG Cuts at: 1872 4936 1872 Size: 3064 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3908 5713 6614 6876 820 Size: 197 926 1965 1805 901 262 1553 Fragments arranged by size: 1965 1805 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5400 493 Size: 4907 2702 SpeI A'CTAG_T Cuts at: 152 3026 152 Size: 2874 4735 SphI G_CATG'C Cuts at: 1132 2333 3542 4967 1132 Size: 1201 1209 1425 3774 Fragments arranged by size: 3774 1425 1209 1201 SspI AAT'ATT Cuts at: 5 52 2079 2142 2971 5533 5 Size: 47 2027 63 829 2562 2081 Fragments arranged by size: 2562 2081 2027 829 63 47 StuI AGG'CCT Cuts at: 1517 2035 1517 Size: 518 7091 StyI C'CwwG_G Cuts at: 513 1065 2004 2200 2391 2616 3306 5048 513 Size: 552 939 196 191 225 690 1742 3074 Fragments arranged by size: 3074 1742 939 690 552 225 196 191 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5636 5653 5806 5991 6436 325 Size: 1255 4056 17 153 185 445 1498 Fragments arranged by size: 4056 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3200 4145 5855 Size: 274 80 33 51 1443 1223 945 1710 Cuts at: 5855 7567 96 Size: 1712 138 Fragments arranged by size: 1712 1710 1443 1223 945 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2499 2531 7569 7601 2499 Size: 32 5038 32 2507 Fragments arranged by size: 5038 2507 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3638 4772 1316 Size: 2322 1134 4153 Fragments arranged by size: 4153 2322 1134 XbaI T'CTAG_A Cuts at: 3508 4330 4894 4945 3508 Size: 822 564 51 6172 Fragments arranged by size: 6172 822 564 51 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2398 4494 4576 2398 Size: 2096 82 5431 Fragments arranged by size: 5431 2096 82 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7609 XmaI C'CCGG_G Cuts at: 1870 4934 1870 Size: 3064 4545 XmnI GAAnn'nnTTC Cuts at: 2094 5738 2094 Size: 3644 3965 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4033 5417 276 Size: 53 83 186 936 2499 1384 2468 Fragments arranged by size: 2499 2468 1384 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BsiWI DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI