Restriction map for
pFN21AB3126
(Linear) (Six-base) MAPSORT of: KIBB3126.seq Check: 2303 from: 1 to: 2904
KIBB3126.orf
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 329 2904
Size: 329 2575
AatII G_ACGT'C
Cuts at: 0 2030 2904
Size: 2030 874
AccI GT'mk_AC
Cuts at: 0 420 2904
Size: 420 2484
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1349 2456 2904
Size: 1349 1107 448
Fragments arranged by size:
1349 1107 448
AleI CACnn'nnGTG
Cuts at: 0 1609 2904
Size: 1609 1295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1556 1880 2005 2080 2904
Size: 1556 324 125 75 824
Fragments arranged by size:
1556 824 324 125 75
AlwNI CAG_nnn'CTG
Cuts at: 0 982 2771 2904
Size: 982 1789 133
Fragments arranged by size:
1789 982 133
ApaLI G'TGCA_C
Cuts at: 0 511 2904
Size: 511 2393
ApoI r'AATT_y
Cuts at: 0 800 2471 2904
Size: 800 1671 433
Fragments arranged by size:
1671 800 433
AseI AT'TA_AT
Cuts at: 0 962 2904
Size: 962 1942
BamHI G'GATC_C
Cuts at: 0 51 2904
Size: 51 2853
BanI G'GyrC_C
Cuts at: 0 211 686 1135 2833 2904
Size: 211 475 449 1698 71
Fragments arranged by size:
1698 475 449 211 71
BanII G_rGCy'C
Cuts at: 0 1412 2753 2904
Size: 1412 1341 151
Fragments arranged by size:
1412 1341 151
BbeI G_GCGC'C
Cuts at: 0 690 2904
Size: 690 2214
BbsI GAAGACnn'nnnn_
Cuts at: 0 927 2692 2904
Size: 927 1765 212
Fragments arranged by size:
1765 927 212
BbvCI CC'TCA_GC
Cuts at: 0 625 1804 2904
Size: 625 1179 1100
Fragments arranged by size:
1179 1100 625
BciVI GTATCCnnnnn_n'
Cuts at: 0 2519 2904
Size: 2519 385
BclI T'GATC_A
Cuts at: 0 1769 2904
Size: 1769 1135
BfrBI ATG'CAT
Cuts at: 0 739 2904
Size: 739 2165
BglI GCCn_nnn'nGGC
Cuts at: 0 2020 2904
Size: 2020 884
Bme1580I G_kGCm'C
Cuts at: 0 515 1189 2904
Size: 515 674 1715
Fragments arranged by size:
1715 674 515
BmgBI CAC'GTC
Cuts at: 0 1572 2904
Size: 1572 1332
BmrI ACTGGGnnnn_n'
Cuts at: 0 2502 2674 2904
Size: 2502 172 230
Fragments arranged by size:
2502 230 172
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2573 2904
Size: 2573 331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 1166 1502 2137 2658 2904
Size: 809 357 336 635 521 246
Fragments arranged by size:
809 635 521 357 336 246
Bpu10I CC'TnA_GC
Cuts at: 0 625 1804 1822 2036 2904
Size: 625 1179 18 214 868
Fragments arranged by size:
1179 868 625 214 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1888 2904
Size: 1888 1016
BsaI GGTCTCn'nnnn_
Cuts at: 0 2595 2904
Size: 2595 309
BsaHI Gr'CG_yC
Cuts at: 0 687 710 1750 2027 2904
Size: 687 23 1040 277 877
Fragments arranged by size:
1040 877 687 277 23
BsaWI w'CCGG_w
Cuts at: 0 660 2904
Size: 660 2244
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2032 2062 2904
Size: 2032 30 842
Fragments arranged by size:
2032 842 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1193 2904
Size: 1193 1711
BseYI C'CCAG_C
Cuts at: 0 561 1282 1411 1810 2017 2179 2904
Size: 561 721 129 399 207 162 725
Fragments arranged by size:
725 721 561 399 207 162 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 1096 1633 2050 2904
Size: 351 745 537 417 854
Fragments arranged by size:
854 745 537 417 351
BsiHKAI G_wGCw'C
Cuts at: 0 515 2084 2753 2904
Size: 515 1569 669 151
Fragments arranged by size:
1569 669 515 151
BsmI GAATG_Cn'
Cuts at: 0 1962 2904
Size: 1962 942
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1562 2904
Size: 1562 1342
Bsp1286I G_dGCh'C
Cuts at: 0 515 1189 1412 2084 2753 2904
Size: 515 674 223 672 669 151
Fragments arranged by size:
674 672 669 515 223 151
BspEI T'CCGG_A
Cuts at: 0 660 2904
Size: 660 2244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 329 2337 2904
Size: 329 2008 567
Fragments arranged by size:
2008 567 329
BsrFI r'CCGG_y
Cuts at: 0 554 2904
Size: 554 2350
BstXI CCAn_nnnn'nTGG
Cuts at: 0 393 618 2199 2872 2904
Size: 393 225 1581 673 32
Fragments arranged by size:
1581 673 393 225 32
BstYI r'GATC_y
Cuts at: 0 51 362 1680 2229 2904
Size: 51 311 1318 549 675
Fragments arranged by size:
1318 675 549 311 51
BstZ17I GTA'TAC
Cuts at: 0 421 2904
Size: 421 2483
Bsu36I CC'TnA_GG
Cuts at: 0 245 2904
Size: 245 2659
BtgI C'CryG_G
Cuts at: 0 195 698 1301 2904
Size: 195 503 603 1603
Fragments arranged by size:
1603 603 503 195
BtsI GCAGTG_nn'
Cuts at: 0 106 1604 1772 2062 2839 2904
Size: 106 1498 168 290 777 65
Fragments arranged by size:
1498 777 290 168 106 65
DrdI GACnn_nn'nnGTC
Cuts at: 0 397 2904
Size: 397 2507
EaeI y'GGCC_r
Cuts at: 0 1586 2012 2333 2904
Size: 1586 426 321 571
Fragments arranged by size:
1586 571 426 321
EarI CTCTTCn'nnn_
Cuts at: 0 851 1466 2213 2904
Size: 851 615 747 691
Fragments arranged by size:
851 747 691 615
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 577 1166 2904
Size: 577 589 1738
Fragments arranged by size:
1738 589 577
EcoICRI GAG'CTC
Cuts at: 0 2751 2904
Size: 2751 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 1166 1349 1502 2137 2456 2658 2904
Size: 809 357 183 153 635 319 202 246
Fragments arranged by size:
809 635 357 319 246 202 183 153
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2730 2891 2904
Size: 2730 161 13
Fragments arranged by size:
2730 161 13
EcoO109I rG'GnC_Cy
Cuts at: 0 1323 2622 2884 2904
Size: 1323 1299 262 20
Fragments arranged by size:
1323 1299 262 20
HaeII r_GCGC'y
Cuts at: 0 690 1599 2904
Size: 690 909 1305
Fragments arranged by size:
1305 909 690
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1071 1103 1297 1329 1722 1754 2032 2064
Size: 1071 32 194 32 393 32 278 32
Cuts at: 2064 2077 2109 2377 2409 2679 2711 2904
Size: 13 32 268 32 270 32 193
Fragments arranged by size:
1071 393 278 270 268 194 193 32
32 32 32 32 32 32 13
KasI G'GCGC_C
Cuts at: 0 686 2904
Size: 686 2218
MfeI C'AATT_G
Cuts at: 0 2651 2904
Size: 2651 253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 767 2904
Size: 767 2137
MscI TGG'CCA
Cuts at: 0 2335 2904
Size: 2335 569
MslI CAynn'nnrTG
Cuts at: 0 323 1538 1609 2870 2904
Size: 323 1215 71 1261 34
Fragments arranged by size:
1261 1215 323 71 34
MspA1I CmG'CkG
Cuts at: 0 561 775 2017 2179 2342 2430 2454 2780
Size: 561 214 1242 162 163 88 24 326
Cuts at: 2780 2904
Size: 124
Fragments arranged by size:
1242 561 326 214 163 162 124 88
24
NarI GG'CG_CC
Cuts at: 0 687 2904
Size: 687 2217
NcoI C'CATG_G
Cuts at: 0 195 1301 2904
Size: 195 1106 1603
Fragments arranged by size:
1603 1106 195
NdeI CA'TA_TG
Cuts at: 0 2376 2904
Size: 2376 528
NsiI A_TGCA'T
Cuts at: 0 741 2904
Size: 741 2163
NspI r_CATG'y
Cuts at: 0 328 1161 1537 2904
Size: 328 833 376 1367
Fragments arranged by size:
1367 833 376 328
PflMI CCAn_nnn'nTGG
Cuts at: 0 118 196 336 1225 2342 2904
Size: 118 78 140 889 1117 562
Fragments arranged by size:
1117 889 562 140 118 78
PfoI T'CCnGG_A
Cuts at: 0 498 1260 2114 2904
Size: 498 762 854 790
Fragments arranged by size:
854 790 762 498
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2307 2339 2904
Size: 2307 32 565
Fragments arranged by size:
2307 565 32
PpuMI rG'GwC_Cy
Cuts at: 0 1323 2884 2904
Size: 1323 1561 20
Fragments arranged by size:
1561 1323 20
PstI C_TGCA'G
Cuts at: 0 605 2453 2904
Size: 605 1848 451
Fragments arranged by size:
1848 605 451
PvuII CAG'CTG
Cuts at: 0 561 775 2179 2342 2430 2454 2904
Size: 561 214 1404 163 88 24 450
Fragments arranged by size:
1404 561 450 214 163 88 24
SacI G_AGCT'C
Cuts at: 0 2753 2904
Size: 2753 151
SapI GCTCTTCn'nnn_
Cuts at: 0 2213 2904
Size: 2213 691
ScaI AGT'ACT
Cuts at: 0 2142 2904
Size: 2142 762
SexAI A'CCwGG_T
Cuts at: 0 207 2904
Size: 207 2697
SfcI C'TryA_G
Cuts at: 0 601 2449 2904
Size: 601 1848 455
Fragments arranged by size:
1848 601 455
SfoI GGC'GCC
Cuts at: 0 688 2904
Size: 688 2216
SmlI C'TyrA_G
Cuts at: 0 1903 2904
Size: 1903 1001
SpeI A'CTAG_T
Cuts at: 0 1021 2904
Size: 1021 1883
SphI G_CATG'C
Cuts at: 0 328 1537 2904
Size: 328 1209 1367
Fragments arranged by size:
1367 1209 328
SspI AAT'ATT
Cuts at: 0 74 137 966 2904
Size: 74 63 829 1938
Fragments arranged by size:
1938 829 74 63
StuI AGG'CCT
Cuts at: 0 30 2904
Size: 30 2874
StyI C'CwwG_G
Cuts at: 0 195 386 611 1301 2904
Size: 195 191 225 690 1603
Fragments arranged by size:
1603 690 225 195 191
TatI w'GTAC_w
Cuts at: 0 1195 2140 2904
Size: 1195 945 764
Fragments arranged by size:
1195 945 764
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 494 526 2904
Size: 494 32 2378
Fragments arranged by size:
2378 494 32
Tth111I GACn'n_nGTC
Cuts at: 0 1633 2767 2904
Size: 1633 1134 137
Fragments arranged by size:
1633 1134 137
XbaI T'CTAG_A
Cuts at: 0 1503 2325 2889 2904
Size: 1503 822 564 15
Fragments arranged by size:
1503 822 564 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 393 2489 2571 2904
Size: 393 2096 82 333
Fragments arranged by size:
2096 393 333 82
XmnI GAAnn'nnTTC
Cuts at: 0 89 2904
Size: 89 2815
ZraI GAC'GTC
Cuts at: 0 2028 2904
Size: 2028 876
Enzymes that do cut:
AarI AatII AccI AcuI AleI AlfI AlwNI ApaLI
ApoI AseI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BfrBI BglI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrFI BstXI BstYI BstZ17I Bsu36I BtgI BtsI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I KasI MfeI MmeI MscI MslI MspA1I NarI
NcoI NdeI NsiI NspI PflMI PfoI PpiI PpuMI
PstI PvuII SacI SapI ScaI SexAI SfcI SfoI
SmlI SpeI SphI SspI StuI StyI TatI TstI
Tth111I XbaI XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI
ApaI AscI AsiSI AvaI AvrII BaeI BcgI BglII
BlpI BmtI BsaAI BsaBI BsiEI BsiWI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BtgZI
ClaI CspCI DraI DraIII EagI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MluI
NaeI NgoMIV NheI NotI NruI PacI PasI PciI
PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI SfiI SgfI SgrAI
SmaI SnaBI SrfI SwaI TaqII XhoI XmaI