(Circular) (Six-base) MAPSORT of: pFN21AB0054.seq Check: 112 from: 1 to: 7813 pFN21AB0054 7813 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5623 278 Size: 53 83 186 936 4087 2468 Fragments arranged by size: 4087 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2778 4216 4503 5156 1937 Size: 841 1438 287 653 4594 Fragments arranged by size: 4594 1438 841 653 287 Acc65I G'GTAC_C Cuts at: 5134 5134 Size: 7813 AclI AA'CG_TT Cuts at: 1563 5940 6313 1563 Size: 4377 373 3063 Fragments arranged by size: 4377 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2408 2925 3514 4053 4139 4589 4964 5876 Size: 776 517 589 539 86 450 375 912 Cuts at: 5876 7260 7528 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 912 776 589 539 517 450 375 268 86 AflII C'TTAA_G Cuts at: 820 1017 3347 820 Size: 197 2330 5286 Fragments arranged by size: 5286 2330 197 AflIII A'CryG_T Cuts at: 1203 3230 4705 6712 7689 1203 Size: 2027 1475 2007 977 1327 Fragments arranged by size: 2027 2007 1475 1327 977 AgeI A'CCGG_T Cuts at: 6644 6644 Size: 7813 AhdI GACnn_n'nnGTC Cuts at: 6542 6542 Size: 7813 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7813 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7813 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4425 4457 4425 Size: 32 7781 AlwNI CAG_nnn'CTG Cuts at: 5556 7128 5556 Size: 1572 6241 ApaLI G'TGCA_C Cuts at: 2756 5870 7026 2756 Size: 3114 1156 3543 Fragments arranged by size: 3543 3114 1156 ApoI r'AATT_y Cuts at: 1493 2254 2592 3070 3728 3870 5122 5348 5500 Size: 761 338 478 658 142 1252 226 152 Cuts at: 5500 7609 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1252 761 658 478 338 226 152 142 AseI AT'TA_AT Cuts at: 160 1032 3246 3579 4371 6367 160 Size: 872 2214 333 792 1996 1606 Fragments arranged by size: 2214 1996 1606 872 792 333 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7813 AvaI C'yCGr_G Cuts at: 1870 1943 4833 5138 1870 Size: 73 2890 305 4545 Fragments arranged by size: 4545 2890 305 73 BamHI G'GATC_C Cuts at: 5143 5510 5143 Size: 367 7446 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5134 6589 618 Size: 325 217 627 3347 1455 1842 Fragments arranged by size: 3347 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3758 5132 1391 Size: 2367 1374 4072 Fragments arranged by size: 4072 2367 1374 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2004 3034 4911 928 Size: 971 105 1030 1877 3830 Fragments arranged by size: 3830 1877 1030 971 105 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6004 6038 1903 Size: 34 4067 34 3678 Fragments arranged by size: 4067 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3959 5705 6915 3959 Size: 1746 1210 4857 Fragments arranged by size: 4857 1746 1210 BclI T'GATC_A Cuts at: 1548 4012 4093 1548 Size: 2464 81 5268 Fragments arranged by size: 5268 2464 81 BfrBI ATG'CAT Cuts at: 2318 3121 2318 Size: 803 7010 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6424 136 Size: 107 122 71 631 605 4752 1525 Fragments arranged by size: 4752 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2192 2800 7808 2192 Size: 608 5008 2197 Fragments arranged by size: 5008 2197 608 BlpI GC'TnA_GC Cuts at: 4041 5230 4041 Size: 1189 6624 Bme1580I G_kGCm'C Cuts at: 1790 2728 2760 4158 4764 5874 7030 1790 Size: 938 32 1398 606 1110 1156 2573 Fragments arranged by size: 2573 1398 1156 1110 938 606 32 BmgBI CAC'GTC Cuts at: 1318 4708 1318 Size: 3390 4423 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5544 6502 442 Size: 416 53 83 399 24 4127 958 1753 Fragments arranged by size: 4127 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7813 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2137 4452 6473 1239 Size: 898 2315 2021 2579 Fragments arranged by size: 2579 2315 2021 898 Bpu10I CC'TnA_GC Cuts at: 3752 4861 3752 Size: 1109 6704 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4892 5938 6803 7101 4892 Size: 1046 865 298 5604 Fragments arranged by size: 5604 1046 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6476 882 Size: 617 4977 2219 Fragments arranged by size: 4977 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5604 493 Size: 711 4400 2702 Fragments arranged by size: 4400 2702 711 BsaBI GATnn'nnATC Cuts at: 7807 7807 Size: 7813 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 5620 6002 6637 Size: 53 83 186 936 476 3611 382 635 Cuts at: 6637 275 Size: 1451 Fragments arranged by size: 3611 1451 936 635 476 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6246 6644 6918 7065 7476 6246 Size: 398 274 147 411 6583 Fragments arranged by size: 6583 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4425 4455 1178 Size: 30 165 30 3022 30 4536 Fragments arranged by size: 4536 3022 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4272 4451 1186 Size: 3086 179 4548 Fragments arranged by size: 4548 3086 179 BseYI C'CCAG_C Cuts at: 3961 4848 4921 7016 7512 3961 Size: 887 73 2095 496 4262 Fragments arranged by size: 4262 2095 887 496 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7240 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5263 6024 7052 1540 Size: 275 186 3262 761 1028 2301 Fragments arranged by size: 3262 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2760 5874 5959 7030 2760 Size: 3114 85 1071 3543 Fragments arranged by size: 3543 3114 1071 85 BsmI GAATG_Cn' Cuts at: 5324 5417 5324 Size: 93 7720 BsmBI CGTCTCn'nnnn_ Cuts at: 2330 2419 2522 2330 Size: 89 103 7621 Fragments arranged by size: 7621 103 89 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2728 2760 3758 4158 4764 5132 5874 Size: 399 938 32 998 400 606 368 742 Cuts at: 5874 5959 7030 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 998 938 742 606 400 399 368 85 32 BspEI T'CCGG_A Cuts at: 7476 7476 Size: 7813 BspHI T'CATG_A Cuts at: 5702 7432 5702 Size: 1730 6083 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2478 5168 844 Size: 1634 2690 3489 Fragments arranged by size: 3489 2690 1634 BsrBI CCG'CTC Cuts at: 1394 1823 5026 5700 1394 Size: 429 3203 674 3507 Fragments arranged by size: 3507 3203 674 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2318 3385 3841 3960 4396 6308 6482 Size: 1183 1070 1067 456 119 436 1912 174 Cuts at: 6482 65 Size: 1396 Fragments arranged by size: 1912 1396 1183 1070 1067 456 436 174 119 BsrFI r'CCGG_y Cuts at: 1700 1810 6457 6644 1700 Size: 110 4647 187 2869 Fragments arranged by size: 4647 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2268 96 Size: 2172 5641 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7813 BssSI C'ACGA_G Cuts at: 5873 6885 5873 Size: 1012 6801 BstAPI GCAn_nnn'nTGC Cuts at: 794 3564 794 Size: 2770 5043 BstBI TT'CG_AA Cuts at: 2252 3139 6617 2252 Size: 887 3478 3448 Fragments arranged by size: 3478 3448 887 BstEII G'GTnAC_C Cuts at: 1184 4482 1184 Size: 3298 4515 BstXI CCAn_nnnn'nTGG Cuts at: 3866 4774 3866 Size: 908 6905 BstYI r'GATC_y Cuts at: 1972 2192 2800 5143 5510 5895 5912 7364 7450 Size: 220 608 2343 367 385 17 1452 86 Cuts at: 7450 7462 7808 1972 Size: 12 346 1977 Fragments arranged by size: 2343 1977 1452 608 385 367 346 220 86 17 12 BstZ17I GTA'TAC Cuts at: 2779 4504 2779 Size: 1725 6088 BtgI C'CryG_G Cuts at: 513 1065 2004 2016 4800 513 Size: 552 939 12 2784 3526 Fragments arranged by size: 3526 2784 939 552 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7074 Fragments arranged by size: 7074 683 56 BtsI GCAGTG_nn' Cuts at: 2101 2461 2757 2902 3227 5329 6123 6143 2101 Size: 360 296 145 325 2102 794 20 3771 Fragments arranged by size: 3771 2102 794 360 325 296 145 20 ClaI AT'CG_AT Cuts at: 1554 2343 4137 1554 Size: 789 1794 5230 Fragments arranged by size: 5230 1794 789 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7778 DraI TTT'AAA Cuts at: 5117 5117 Size: 7813 DraIII CAC_nnn'GTG Cuts at: 2752 2752 Size: 7813 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6820 809 Size: 507 5504 1802 Fragments arranged by size: 5504 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 5260 6149 Size: 54 1425 50 275 18 198 3232 889 Cuts at: 6149 7778 8 Size: 1629 43 Fragments arranged by size: 3232 1629 1425 889 275 198 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5260 1537 Size: 275 3448 4090 Fragments arranged by size: 4090 3448 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2427 3000 5743 1353 Size: 1074 573 2743 3423 Fragments arranged by size: 3423 2743 1074 573 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6400 6774 6920 387 Size: 1064 138 4811 374 146 1280 Fragments arranged by size: 4811 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2137 2408 2925 3514 4053 4139 4452 Size: 393 505 271 517 589 539 86 313 Cuts at: 4452 4589 4964 5876 6473 7260 7528 1239 Size: 137 375 912 597 787 268 1524 Fragments arranged by size: 1524 912 787 597 589 539 517 505 393 375 313 271 268 137 86 EcoNI CCTnn'n_nnAGG Cuts at: 4254 4254 Size: 7813 EcoO109I rG'GnC_Cy Cuts at: 4794 4794 Size: 7813 EcoRI G'AATT_C Cuts at: 3870 5122 5500 3870 Size: 1252 378 6183 Fragments arranged by size: 6183 1252 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4223 4223 Size: 7813 FspI TGC'GCA Cuts at: 6319 6319 Size: 7813 HaeII r_GCGC'y Cuts at: 1678 3912 6960 1678 Size: 2234 3048 2531 Fragments arranged by size: 3048 2531 2234 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2728 2760 3152 3184 4130 Size: 32 135 32 1156 32 392 32 946 Cuts at: 4130 4162 4231 4263 6454 6486 6528 6560 1373 Size: 32 69 32 2191 32 42 32 2626 Fragments arranged by size: 2626 2191 1156 946 392 135 69 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5157 5403 669 Size: 988 281 3219 246 3079 Fragments arranged by size: 3219 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2244 2406 748 Size: 1496 162 6155 Fragments arranged by size: 6155 1496 162 HpaI GTT'AAC Cuts at: 5403 5403 Size: 7813 KpnI G_GTAC'C Cuts at: 5138 5138 Size: 7813 MfeI C'AATT_G Cuts at: 2298 2523 5412 2298 Size: 225 2889 4699 Fragments arranged by size: 4699 2889 225 MluI A'CGCG_T Cuts at: 3230 7689 3230 Size: 4459 3354 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3687 6927 7111 1305 Size: 2382 3240 184 2007 Fragments arranged by size: 3240 2382 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5991 Fragments arranged by size: 5991 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3864 4161 4772 4913 5771 6130 6289 Size: 648 2698 297 611 141 858 359 159 Cuts at: 6289 518 Size: 2042 Fragments arranged by size: 2698 2042 858 648 611 359 297 159 141 MspA1I CmG'CkG Cuts at: 1606 2093 2381 3848 4031 4723 5060 5229 5906 Size: 487 288 1467 183 692 337 169 677 Cuts at: 5906 7054 7299 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1467 1148 692 677 487 337 288 245 183 169 NcoI C'CATG_G Cuts at: 513 1065 2004 4800 513 Size: 552 939 2796 3526 Fragments arranged by size: 3526 2796 939 552 NdeI CA'TA_TG Cuts at: 387 3601 387 Size: 3214 4599 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7813 NotI GC'GGCC_GC Cuts at: 5260 5260 Size: 7813 NsiI A_TGCA'T Cuts at: 2320 3123 2320 Size: 803 7010 NspI r_CATG'y Cuts at: 1132 1229 2110 2318 2511 3715 5171 6716 1132 Size: 97 881 208 193 1204 1456 1545 2229 Fragments arranged by size: 2229 1545 1456 1204 881 208 193 97 PasI CC'CwG_GG Cuts at: 1348 2482 3971 1348 Size: 1134 1489 5190 Fragments arranged by size: 5190 1489 1134 PciI A'CATG_T Cuts at: 6712 6712 Size: 7813 PflMI CCAn_nnn'nTGG Cuts at: 1108 3764 3836 1108 Size: 2656 72 5085 Fragments arranged by size: 5085 2656 72 PmeI GTTT'AAAC Cuts at: 5117 5117 Size: 7813 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4425 4457 5882 5914 7395 7427 4425 Size: 32 1425 32 1481 32 4811 Fragments arranged by size: 4811 1481 1425 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4794 4794 Size: 7813 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7813 PsiI TTA'TAA Cuts at: 2922 3126 5383 2922 Size: 204 2257 5352 Fragments arranged by size: 5352 2257 204 PstI C_TGCA'G Cuts at: 830 3090 3108 3396 5165 830 Size: 2260 18 288 1769 3478 Fragments arranged by size: 3478 2260 1769 288 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7813 PvuII CAG'CTG Cuts at: 2093 3848 4031 4723 5060 2093 Size: 1755 183 692 337 4846 Fragments arranged by size: 4846 1755 692 337 183 SalI G'TCGA_C Cuts at: 1936 5155 1936 Size: 3219 4594 SanDI GG'GwC_CC Cuts at: 4794 4794 Size: 7813 SapI GCTCTTCn'nnn_ Cuts at: 2427 2427 Size: 7813 SbfI CC_TGCA'GG Cuts at: 5165 5165 Size: 7813 ScaI AGT'ACT Cuts at: 6061 7773 6061 Size: 1712 6101 SfcI C'TryA_G Cuts at: 826 1045 2818 3086 3104 3392 5036 5161 6296 Size: 219 1773 268 18 288 1644 125 1135 Cuts at: 6296 6977 7168 826 Size: 681 191 1471 Fragments arranged by size: 1773 1644 1471 1135 681 288 268 219 191 125 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7813 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7813 SmaI CCC'GGG Cuts at: 1872 5140 1872 Size: 3268 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3347 4833 4871 5917 6818 7080 Size: 197 926 1404 1486 38 1046 901 262 Cuts at: 7080 820 Size: 1553 Fragments arranged by size: 1553 1486 1404 1046 926 901 262 197 38 SnaBI TAC'GTA Cuts at: 493 5604 493 Size: 5111 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7813 SphI G_CATG'C Cuts at: 1132 2110 3715 5171 1132 Size: 978 1605 1456 3774 Fragments arranged by size: 3774 1605 1456 978 SspI AAT'ATT Cuts at: 5 52 3307 4630 5737 5 Size: 47 3255 1323 1107 2081 Fragments arranged by size: 3255 2081 1323 1107 47 StuI AGG'CCT Cuts at: 1517 2793 1517 Size: 1276 6537 StyI C'CwwG_G Cuts at: 513 1065 2004 2370 2502 4800 5252 513 Size: 552 939 366 132 2298 452 3074 Fragments arranged by size: 3074 2298 939 552 452 366 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2963 5840 5857 6010 6195 6640 325 Size: 1255 1383 2877 17 153 185 445 1498 Fragments arranged by size: 2877 1498 1383 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2268 2401 2427 Size: 274 80 33 51 1443 291 133 26 Cuts at: 2427 2442 3082 4588 6059 7771 96 Size: 15 640 1506 1471 1712 138 Fragments arranged by size: 1712 1506 1471 1443 640 291 274 138 133 80 51 33 26 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3967 3999 7773 7805 3967 Size: 32 3774 32 3975 Fragments arranged by size: 3975 3774 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7813 XbaI T'CTAG_A Cuts at: 4933 5149 4933 Size: 216 7597 XhoI C'TCGA_G Cuts at: 1943 4833 1943 Size: 2890 4923 XmaI C'CCGG_G Cuts at: 1870 5138 1870 Size: 3268 4545 XmnI GAAnn'nnTTC Cuts at: 5942 5942 Size: 7813 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5621 276 Size: 53 83 186 936 4087 2468 Fragments arranged by size: 4087 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI