Restriction map for
pFN21AB7265
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI AspEI AvaI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BsaAI BsaBI BsaI
BsiEI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KasI Ksp632I KspI MamI MfeI
MluI MseI MslI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tru9I Tsp509I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 670 GGTACC G^GTACC
AccII 611 CGCG CG^CG
AcyI 540 GACGCC G[GA]^CG[CT]C
AlwNI 562 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 670 GGTACC G^GTACC
AspI 916 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 759 TGGCCA TGG^CCA
BanII 886 GAGCTC G[GA]GC[CT]^C
BbsI 553 GAAGAC GAAGAC(2/6)
BfaI 208 CTAG C^TAG
BglII 524 AGATCT A^GATCT
BpuAI 553 GAAGAC GAAGAC(2/6)
BsaHI 540 GACGCC G[GA]^CG[CT]C
BsgI 685 GTGCAG GTGCAG(16/14)
BsmI 856 GAATGC GAATGC(1/-1)
BstUI 611 CGCG CG^CG
BstYI 524 AGATCT [GA]^GATC[CT]
EaeI 759 TGGCCA [CT]^GGCC[GA]
EcoRV 580 GATATC GAT^ATC
HaeII 478 AGCGCC [GA]GCGC^[CT]
Hin1I 540 GACGCC G[GA]^CG[CT]C
KpnI 670 GGTACC GGTAC^C
MaeI 208 CTAG C^TAG
MaeII 839 ACGT A^CGT
MflI 524 AGATCT [GA]^GATC[CT]
MluNI 759 TGGCCA TGG^CCA
MscI 759 TGGCCA TGG^CCA
MspA1I 483 CAGCTG C[AC]G^C[GT]G
MvnI 611 CGCG CG^CG
NaeI 488 GCCGGC GCC^GGC
NgoMIV 488 GCCGGC G^CCGGC
NspI 98 ACATGC [GA]CATG^[CT]
PflFI 916 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 483 CAGCTG CAG^CTG
SacI 886 GAGCTC GAGCT^C
SfcI 831 CTACAG C^T[GA][CT]AG
StuI 827 AGGCCT AGG^CCT
TfiI 146 GAATC G^A[AT]TC
TspRI 469 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 916 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 524 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 270 TCCGGA T^CCGGA
923 TCCGGA
Asp700I 15 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
522 GAAGATCTTC
AspHI 886 GAGCTC G[AT]GC[AT]^C
931 GAGCAC
BcnI 92 CCCGG CC^[GC]GG
416 CCGGG
BmyI 886 GAGCTC G[AGT]GC[ACT]^C
931 GAGCAC
BpmI 403 CTGGAG CTGGAG(16/14)
910 CTGGAG
BsaWI 270 TCCGGA [AT]^CCGG[AT]
923 TCCGGA
BseAI 270 TCCGGA T^CCGGA
923 TCCGGA
BseRI 655 CTCCTC GAGGAG(10/8)
800 GAGGAG
BsiHKAI 886 GAGCTC G[AT]GC[AT]^C
931 GAGCAC
Bsp1286I 886 GAGCTC G[AGT]GC[ACT]^C
931 GAGCAC
BspEI 270 TCCGGA T^CCGGA
923 TCCGGA
BspMI 1011 ACCTGC ACCTGC(4/8)
681 GCAGGT
BsrFI 190 ACCGGC [GA]^CCGG[CT]
488 GCCGGC
BsrI 762 CCAGT ACTGG(1/-1)
951 CCAGT
Cfr10I 190 ACCGGC [GA]^CCGG[CT]
488 GCCGGC
DpnII 249 GATC ^GATC
525 GATC
DraII 914 AGGACCT [GA]G^G[ACGT]CC[CT]
965 GGGTCCC
EcoNI 288 CCTCGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
348 CCTGCTGAAGG
EcoO109I 914 AGGACCT [GA]G^G[ACGT]CC[CT]
965 GGGTCCC
MaeIII 332 GTCAC ^GT[ACGT]AC
538 GTGAC
MboI 249 GATC ^GATC
525 GATC
MroI 270 TCCGGA T^CCGGA
923 TCCGGA
NciI 92 CCCGG CC^[GC]GG
416 CCGGG
NdeII 249 GATC ^GATC
525 GATC
PleI 817 GACTC GAGTC(4/5)
122 GAGTC
PpuMI 914 AGGACCT [GA]G^G[AT]CC[CT]
965 GGGTCCC
Sau3AI 249 GATC ^GATC
525 GATC
SfaNI 946 GATGC GCATC(5/9)
164 GCATC
Tsp45I 332 GTCAC ^GT[GC]AC
538 GTGAC
XmnI 15 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
522 GAAGATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BbvI BlpI Bpu1102I BsaJI BsiYI
BslI BsmFI BssKI BstF5I BstNI
Cac8I CelII CfoI Cfr13I DdeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MboII
MnlI MspI MvaI MwoI NlaIII
NlaIV RsaI Sau96I ScrFI StyI
TseI