Restriction map for
pFN21AB7265
(Linear) (Six-base) MAPSORT of: KIBB7265.seq Check: 6626 from: 1 to: 1056
KIBB7265.orf
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 672 1020 1056
Size: 672 348 36
Fragments arranged by size:
672 348 36
Acc65I G'GTAC_C
Cuts at: 0 670 1056
Size: 670 386
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 664 968 1056
Size: 373 291 304 88
Fragments arranged by size:
373 304 291 88
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 710 1056
Size: 710 346
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 7 39 1056
Size: 7 32 1017
Fragments arranged by size:
1017 32 7
AlwNI CAG_nnn'CTG
Cuts at: 0 567 1056
Size: 567 489
BanI G'GyrC_C
Cuts at: 0 451 670 714 1056
Size: 451 219 44 342
Fragments arranged by size:
451 342 219 44
BanII G_rGCy'C
Cuts at: 0 890 1056
Size: 890 166
BbsI GAAGACnn'nnnn_
Cuts at: 0 560 1056
Size: 560 496
BglII A'GATC_T
Cuts at: 0 524 1056
Size: 524 532
BlpI GC'TnA_GC
Cuts at: 0 34 505 884 1056
Size: 34 471 379 172
Fragments arranged by size:
471 379 172 34
BmgBI CAC'GTC
Cuts at: 0 840 1056
Size: 840 216
BmrI ACTGGGnnnn_n'
Cuts at: 0 945 1056
Size: 945 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 931 1056
Size: 424 507 125
Fragments arranged by size:
507 424 125
BsaHI Gr'CG_yC
Cuts at: 0 541 1056
Size: 541 515
BsaWI w'CCGG_w
Cuts at: 0 270 923 1056
Size: 270 653 133
Fragments arranged by size:
653 270 133
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 7 37 802 832 1056
Size: 7 30 765 30 224
Fragments arranged by size:
765 224 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 646 815 1056
Size: 646 169 241
Fragments arranged by size:
646 241 169
BseYI C'CCAG_C
Cuts at: 0 606 849 976 1056
Size: 606 243 127 80
Fragments arranged by size:
606 243 127 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1056
Size: 706 350
BsiHKAI G_wGCw'C
Cuts at: 0 890 935 1056
Size: 890 45 121
Fragments arranged by size:
890 121 45
BsmI GAATG_Cn'
Cuts at: 0 862 1056
Size: 862 194
Bsp1286I G_dGCh'C
Cuts at: 0 890 935 1056
Size: 890 45 121
Fragments arranged by size:
890 121 45
BspEI T'CCGG_A
Cuts at: 0 270 923 1056
Size: 270 653 133
Fragments arranged by size:
653 270 133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 672 1020 1056
Size: 672 348 36
Fragments arranged by size:
672 348 36
BsrFI r'CCGG_y
Cuts at: 0 190 488 1056
Size: 190 298 568
Fragments arranged by size:
568 298 190
BstYI r'GATC_y
Cuts at: 0 524 1056
Size: 524 532
BtsI GCAGTG_nn'
Cuts at: 0 468 1056
Size: 468 588
EaeI y'GGCC_r
Cuts at: 0 759 1056
Size: 759 297
EcoICRI GAG'CTC
Cuts at: 0 888 1056
Size: 888 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 424 664 931 968 1056
Size: 373 51 240 267 37 88
Fragments arranged by size:
373 267 240 88 51 37
EcoNI CCTnn'n_nnAGG
Cuts at: 0 292 352 1056
Size: 292 60 704
Fragments arranged by size:
704 292 60
EcoO109I rG'GnC_Cy
Cuts at: 0 915 966 1056
Size: 915 51 90
Fragments arranged by size:
915 90 51
EcoRV GAT'ATC
Cuts at: 0 582 1056
Size: 582 474
HaeII r_GCGC'y
Cuts at: 0 482 1056
Size: 482 574
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 131 163 195 802 834 937 969 1056
Size: 131 32 32 607 32 103 32 87
Fragments arranged by size:
607 131 103 87 32 32 32 32
KpnI G_GTAC'C
Cuts at: 0 674 1056
Size: 674 382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 1056
Size: 746 310
MscI TGG'CCA
Cuts at: 0 761 1056
Size: 761 295
MspA1I CmG'CkG
Cuts at: 0 485 1056
Size: 485 571
NaeI GCC'GGC
Cuts at: 0 490 1056
Size: 490 566
NgoMIV G'CCGG_C
Cuts at: 0 488 1056
Size: 488 568
NspI r_CATG'y
Cuts at: 0 102 1056
Size: 102 954
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 1056
Size: 7 32 1017
Fragments arranged by size:
1017 32 7
PpuMI rG'GwC_Cy
Cuts at: 0 915 966 1056
Size: 915 51 90
Fragments arranged by size:
915 90 51
PvuII CAG'CTG
Cuts at: 0 485 1056
Size: 485 571
SacI G_AGCT'C
Cuts at: 0 890 1056
Size: 890 166
SanDI GG'GwC_CC
Cuts at: 0 966 1056
Size: 966 90
SfcI C'TryA_G
Cuts at: 0 831 1056
Size: 831 225
StuI AGG'CCT
Cuts at: 0 829 1056
Size: 829 227
StyI C'CwwG_G
Cuts at: 0 77 293 891 1016 1056
Size: 77 216 598 125 40
Fragments arranged by size:
598 216 125 77 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 841 1056
Size: 841 215
Tth111I GACn'n_nGTC
Cuts at: 0 919 1056
Size: 919 137
XmnI GAAnn'nnTTC
Cuts at: 0 19 526 1056
Size: 19 507 530
Fragments arranged by size:
530 507 19
Enzymes that do cut:
AarI Acc65I AcuI AlfI AloI AlwNI BanI BanII
BbsI BglII BlpI BmgBI BmrI BpmI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrFI BstYI BtsI EaeI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV HaeII Hin4I KpnI MmeI MscI MspA1I
NaeI NgoMIV NspI PpiI PpuMI PvuII SacI SanDI
SfcI StuI StyI TaqII Tth111I XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI Bme1580I BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsiEI BsiWI BsmBI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoRI FalI FseI FspI FspAI
HincII HindIII HpaI KasI MfeI MluI MslI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI XbaI XcmI XhoI XmaI ZraI