(Circular) (Six-base) MAPSORT of: pFN21AB8168.seq Check: 2273 from: 1 to: 5617 pFN21AB8168 5617 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3427 278 Size: 53 83 186 936 1891 2468 Fragments arranged by size: 2468 1891 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2960 1937 Size: 1023 4594 Acc65I G'GTAC_C Cuts at: 2230 2938 2230 Size: 708 4909 AclI AA'CG_TT Cuts at: 1563 3744 4117 1563 Size: 2181 373 3063 Fragments arranged by size: 3063 2181 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2832 3680 5064 5332 1632 Size: 1200 848 1384 268 1917 Fragments arranged by size: 1917 1384 1200 848 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5420 AflIII A'CryG_T Cuts at: 1203 4516 5493 1203 Size: 3313 977 1327 Fragments arranged by size: 3313 1327 977 AgeI A'CCGG_T Cuts at: 2719 4448 2719 Size: 1729 3888 AhdI GACnn_n'nnGTC Cuts at: 4346 4346 Size: 5617 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5617 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2274 2373 1184 Size: 1090 99 4428 Fragments arranged by size: 4428 1090 99 AlwNI CAG_nnn'CTG Cuts at: 3360 4932 3360 Size: 1572 4045 ApaLI G'TGCA_C Cuts at: 3674 4830 3674 Size: 1156 4461 ApoI r'AATT_y Cuts at: 1493 2926 3152 3304 5413 1493 Size: 1433 226 152 2109 1697 Fragments arranged by size: 2109 1697 1433 226 152 AseI AT'TA_AT Cuts at: 160 1032 4171 160 Size: 872 3139 1606 Fragments arranged by size: 3139 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5617 AvaI C'yCGr_G Cuts at: 1870 1943 2021 2942 1870 Size: 73 78 921 4545 Fragments arranged by size: 4545 921 78 73 BamHI G'GATC_C Cuts at: 2947 3314 2947 Size: 367 5250 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2230 2513 2618 2680 2938 Size: 325 217 627 443 283 105 62 258 Cuts at: 2938 4393 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 443 325 283 258 217 105 62 BanII G_rGCy'C Cuts at: 1391 2017 2666 2846 2936 1391 Size: 626 649 180 90 4072 Fragments arranged by size: 4072 649 626 180 90 BbeI G_GCGC'C Cuts at: 2517 2684 2517 Size: 167 5450 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2545 2785 928 Size: 971 646 240 3760 Fragments arranged by size: 3760 971 646 240 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3808 3842 1903 Size: 34 1871 34 3678 Fragments arranged by size: 3678 1871 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3509 4719 3509 Size: 1210 4407 BclI T'GATC_A Cuts at: 1548 1548 Size: 5617 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4228 136 Size: 107 122 71 631 605 2556 1525 Fragments arranged by size: 2556 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5612 5612 Size: 5617 BlpI GC'TnA_GC Cuts at: 2262 3034 2262 Size: 772 4845 Bme1580I G_kGCm'C Cuts at: 1790 2621 3678 4834 1790 Size: 831 1057 1156 2573 Fragments arranged by size: 2573 1156 1057 831 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5617 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2229 3348 4306 Size: 416 53 83 399 24 812 1119 958 Cuts at: 4306 442 Size: 1753 Fragments arranged by size: 1753 1119 958 812 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5617 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2047 2124 2047 Size: 77 5540 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4277 1239 Size: 3038 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2119 3742 4607 4905 2119 Size: 1623 865 298 2831 Fragments arranged by size: 2831 1623 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2048 2184 4280 882 Size: 617 549 136 2096 2219 Fragments arranged by size: 2219 2096 617 549 136 BsaAI yAC'GTr Cuts at: 493 1204 3408 493 Size: 711 2204 2702 Fragments arranged by size: 2702 2204 711 BsaBI GATnn'nnATC Cuts at: 5611 5611 Size: 5617 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2514 2681 3424 3806 Size: 53 83 186 936 981 167 743 382 Cuts at: 3806 4441 275 Size: 635 1451 Fragments arranged by size: 1451 981 936 743 635 382 186 167 83 53 BsaWI w'CCGG_w Cuts at: 2193 2674 2719 4050 4448 4722 4869 5280 2193 Size: 481 45 1331 398 274 147 411 2530 Fragments arranged by size: 2530 1331 481 411 398 274 147 45 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2709 2739 2901 2931 1178 Size: 30 165 30 1306 30 162 30 3864 Fragments arranged by size: 3864 1306 165 162 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2761 1186 Size: 837 738 4042 Fragments arranged by size: 4042 837 738 BseYI C'CCAG_C Cuts at: 2493 2659 4820 5316 2493 Size: 166 2161 496 2794 Fragments arranged by size: 2794 2161 496 166 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5044 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3067 3828 4856 1540 Size: 275 186 1066 761 1028 2301 Fragments arranged by size: 2301 1066 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2017 2727 3678 3763 4834 2017 Size: 710 951 85 1071 2800 Fragments arranged by size: 2800 1071 951 710 85 BsmI GAATG_Cn' Cuts at: 2396 3128 3221 2396 Size: 732 93 4792 Fragments arranged by size: 4792 732 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2017 2621 2666 2727 2846 2936 3678 Size: 399 227 604 45 61 119 90 742 Cuts at: 3678 3763 4834 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 604 399 227 119 90 85 61 45 BspEI T'CCGG_A Cuts at: 2674 5280 2674 Size: 2606 3011 BspHI T'CATG_A Cuts at: 3506 5236 3506 Size: 1730 3887 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2972 844 Size: 2128 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2511 3504 1394 Size: 429 688 993 3507 Fragments arranged by size: 3507 993 688 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4112 4286 65 Size: 1183 2864 174 1396 Fragments arranged by size: 2864 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2719 4261 4448 1700 Size: 110 909 1542 187 2869 Fragments arranged by size: 2869 1542 909 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5617 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5617 BssSI C'ACGA_G Cuts at: 3677 4689 3677 Size: 1012 4605 BstAPI GCAn_nnn'nTGC Cuts at: 794 2405 794 Size: 1611 4006 BstBI TT'CG_AA Cuts at: 4421 4421 Size: 5617 BstEII G'GTnAC_C Cuts at: 1184 2734 1184 Size: 1550 4067 BstXI CCAn_nnnn'nTGG Cuts at: 2065 2065 Size: 5617 BstYI r'GATC_y Cuts at: 1972 2255 2280 2947 3314 3699 3716 5168 5254 Size: 283 25 667 367 385 17 1452 86 Cuts at: 5254 5266 5612 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 667 385 367 346 283 86 25 17 12 BtgI C'CryG_G Cuts at: 513 1065 2295 2605 2622 2634 513 Size: 552 1230 310 17 12 3496 Fragments arranged by size: 3496 1230 552 310 17 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2557 487 Size: 683 56 1331 3547 Fragments arranged by size: 3547 1331 683 56 BtsI GCAGTG_nn' Cuts at: 3133 3927 3947 3133 Size: 794 20 4803 Fragments arranged by size: 4803 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5617 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5582 DraI TTT'AAA Cuts at: 2921 2921 Size: 5617 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4624 809 Size: 507 3308 1802 Fragments arranged by size: 3308 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 2602 3064 Size: 54 1425 50 275 18 318 454 462 Cuts at: 3064 3953 5582 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 462 454 318 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3064 1537 Size: 275 1252 4090 Fragments arranged by size: 4090 1252 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3547 1353 Size: 2194 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2731 4204 4578 4724 387 Size: 1064 138 1142 1473 374 146 1280 Fragments arranged by size: 1473 1280 1142 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2015 2015 Size: 5617 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2832 3680 4277 5064 5332 1239 Size: 393 1200 848 597 787 268 1524 Fragments arranged by size: 1524 1200 848 787 597 393 268 EcoRI G'AATT_C Cuts at: 2926 3304 2926 Size: 378 5239 FspI TGC'GCA Cuts at: 4123 4123 Size: 5617 HaeII r_GCGC'y Cuts at: 1678 2185 2517 2684 2699 4764 1678 Size: 507 332 167 15 2065 2531 Fragments arranged by size: 2531 2065 507 332 167 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2181 2213 2284 2316 2709 Size: 32 135 32 609 32 71 32 393 Cuts at: 2709 2741 4258 4290 4332 4364 1373 Size: 32 1517 32 42 32 2626 Fragments arranged by size: 2626 1517 609 393 135 71 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2961 3207 669 Size: 988 281 1023 246 3079 Fragments arranged by size: 3079 1023 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5617 HpaI GTT'AAC Cuts at: 3207 3207 Size: 5617 KasI G'GCGC_C Cuts at: 2513 2680 2513 Size: 167 5450 KpnI G_GTAC'C Cuts at: 2234 2942 2234 Size: 708 4909 MfeI C'AATT_G Cuts at: 3216 3216 Size: 5617 MluI A'CGCG_T Cuts at: 5493 5493 Size: 5617 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4731 4915 1305 Size: 3426 184 2007 Fragments arranged by size: 3426 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2150 10 Size: 54 1425 343 318 3477 Fragments arranged by size: 3477 1425 343 318 54 MslI CAynn'nnrTG Cuts at: 518 1166 2063 3575 3934 4093 518 Size: 648 897 1512 359 159 2042 Fragments arranged by size: 2042 1512 897 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2497 2584 3033 3710 4858 5103 1606 Size: 891 87 449 677 1148 245 2120 Fragments arranged by size: 2120 1148 891 677 449 245 87 NarI GG'CG_CC Cuts at: 2514 2681 2514 Size: 167 5450 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5065 NdeI CA'TA_TG Cuts at: 387 387 Size: 5617 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5617 NotI GC'GGCC_GC Cuts at: 3064 3064 Size: 5617 NspI r_CATG'y Cuts at: 1132 1229 2975 4520 1132 Size: 97 1746 1545 2229 Fragments arranged by size: 2229 1746 1545 97 PasI CC'CwG_GG Cuts at: 1348 2541 1348 Size: 1193 4424 PciI A'CATG_T Cuts at: 4516 4516 Size: 5617 PflMI CCAn_nnn'nTGG Cuts at: 1108 2146 1108 Size: 1038 4579 PmeI GTTT'AAAC Cuts at: 2921 2921 Size: 5617 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3686 3718 5199 5231 3686 Size: 32 1481 32 4072 Fragments arranged by size: 4072 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5617 PsiI TTA'TAA Cuts at: 3187 3187 Size: 5617 PstI C_TGCA'G Cuts at: 830 2289 2969 830 Size: 1459 680 3478 Fragments arranged by size: 3478 1459 680 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5617 RsrII CG'GwC_CG Cuts at: 2717 2717 Size: 5617 SacI G_AGCT'C Cuts at: 2017 2017 Size: 5617 SalI G'TCGA_C Cuts at: 1936 2959 1936 Size: 1023 4594 SbfI CC_TGCA'GG Cuts at: 2969 2969 Size: 5617 ScaI AGT'ACT Cuts at: 3865 5577 3865 Size: 1712 3905 SfcI C'TryA_G Cuts at: 826 1045 2285 2884 2965 4100 4781 4972 826 Size: 219 1240 599 81 1135 681 191 1471 Fragments arranged by size: 1471 1240 1135 681 599 219 191 81 SfoI GGC'GCC Cuts at: 2515 2682 2515 Size: 167 5450 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5617 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5617 SmaI CCC'GGG Cuts at: 1872 2944 1872 Size: 1072 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2098 3721 4622 4884 820 Size: 197 926 155 1623 901 262 1553 Fragments arranged by size: 1623 1553 926 901 262 197 155 SnaBI TAC'GTA Cuts at: 493 3408 493 Size: 2915 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5617 SphI G_CATG'C Cuts at: 1132 2975 1132 Size: 1843 3774 SspI AAT'ATT Cuts at: 5 52 3541 5 Size: 47 3489 2081 Fragments arranged by size: 3489 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5617 StyI C'CwwG_G Cuts at: 513 1065 2872 2891 3056 513 Size: 552 1807 19 165 3074 Fragments arranged by size: 3074 1807 552 165 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2647 2779 2869 3644 3661 3814 3999 Size: 1255 1067 132 90 775 17 153 185 Cuts at: 3999 4444 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1067 775 445 185 153 132 90 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2025 3863 5575 Size: 274 80 33 51 1443 48 1838 1712 Cuts at: 5575 96 Size: 138 Fragments arranged by size: 1838 1712 1443 274 138 80 51 48 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5577 5609 5577 Size: 32 5585 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5617 XbaI T'CTAG_A Cuts at: 2953 2953 Size: 5617 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5617 XmaI C'CCGG_G Cuts at: 1870 2942 1870 Size: 1072 4545 XmnI GAAnn'nnTTC Cuts at: 3746 3746 Size: 5617 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3425 276 Size: 53 83 186 936 1891 2468 Fragments arranged by size: 2468 1891 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI RsrII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI