################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII AccIII AclI AcyI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI AspEI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaHI BsaWI BseAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstPI BstXI CelII CfoI Cfr10I ClaI CpoI DraII EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HpaI HpaII KasI MfeI MluI MroI MspI MunI NaeI NarI NciI NdeI NgoMIV NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SbfI SexAI SfiI SfoI SgrAI SmaI SpeI Sse8387I SspBI SspI StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 65 enzymes) # ################################### Acc65I 1327 GGTACC G^GTACC AccII 1866 CGCG CG^CG Alw44I 1977 GTGCAC G^TGCAC AlwI 1897 GGATC GGATC(4/5) AlwNI 1574 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1699 CCTCAGG CC^T[ACGT]AGG ApaLI 1977 GTGCAC G^TGCAC Asp718I 1327 GGTACC G^GTACC AspI 102 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 362 GGTCC G^G[AT]CC BalI 1562 TGGCCA TGG^CCA BglII 955 AGATCT A^GATCT BsaBI 1892 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 777 CTCCTC GAGGAG(10/8) BsiEI 101 CGACCG CG[GA][CT]^CG BsmFI 1478 GGGAC GGGAC(10/14) BsmI 1843 GAATGC GAATGC(1/-1) BspHI 1146 TCATGA T^CATGA BssKI 808 CCTGG ^CC[ACGT]GG Bst1107I 438 GTATAC GTA^TAC BstBI 485 TTCGAA TT^CGAA BstNI 808 CCTGG CC^[AT]GG BstUI 1866 CGCG CG^CG BstYI 955 AGATCT [GA]^GATC[CT] BstZ17I 438 GTATAC GTA^TAC Bsu36I 1699 CCTCAGG CC^T[ACGT]AGG Cfr13I 362 GGTCC G^G[ACGT]CC DraI 903 TTTAAA TTT^AAA DrdI 1043 GACAATCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 1562 TGGCCA [CT]^GGCC[GA] EarI 160 GAAGAG CTCTTC(1/4) Eco81I 1699 CCTCAGG CC^T[ACGT]AGG EcoNI 1695 CCTACCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 852 GAATTC G^AATTC EcoRII 808 CCTGG ^CC[AT]GG HincII 663 GTCGAC GT[CT]^[GA]AC HindII 663 GTCGAC GT[CT]^[GA]AC KpnI 1327 GGTACC GGTAC^C Ksp632I 160 GAAGAG CTCTTC(1/4) KspI 1865 CCGCGG CCGC^GG MamI 1892 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 955 AGATCT [GA]^GATC[CT] MluNI 1562 TGGCCA TGG^CCA MscI 1562 TGGCCA TGG^CCA MvaI 808 CCTGG CC^[AT]GG MvnI 1866 CGCG CG^CG NspV 485 TTCGAA TT^CGAA PflFI 102 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 921 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 34 CAGCTG CAG^CTG RcaI 1146 TCATGA T^CATGA SacI 2071 GAGCTC GAGCT^C SacII 1865 CCGCGG CCGC^GG SalI 663 GTCGAC G^TCGAC SapI 160 GAAGAGC GCTCTTC(1/4) Sau96I 362 GGTCC G^G[ACGT]CC ScaI 732 AGTACT AGT^ACT ScrFI 808 CCTGG CC^[ACGT]GG SfuI 485 TTCGAA TT^CGAA SnaBI 1603 TACGTA TAC^GTA SphI 165 GCATGC GCATG^C Tth111I 102 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 921 CCAATGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 135 CCATGTTGCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 955 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 438 GTATAC GT^[AC][GT]AC 663 GTCGAC AciI 1865 CCGC CCGC(-3/-1) 1867 GCGG AflIII 24 ACATGT A^C[GA][CT]GT 1432 ACATGT AspHI 1977 GTGCAC G[AT]GC[AT]^C 2071 GAGCTC BbsI 948 GAAGAC GAAGAC(2/6) 2101 GAAGAC BciVI 12 GGATAC GTATCC(6/5) 464 GGATAC BfaI 4 CTAG C^TAG 941 CTAG BpuAI 948 GAAGAC GAAGAC(2/6) 2101 GAAGAC BsaAI 15 TACGTG [CT]AC^GT[GA] 1603 TACGTA BsgI 333 CTGCAC GTGCAG(16/14) 1714 CTGCAC BsiHKAI 1977 GTGCAC G[AT]GC[AT]^C 2071 GAGCTC BsiYI 921 CCAATGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1695 CCTACCTCAGG BslI 921 CCAATGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1695 CCTACCTCAGG BspLU11I 24 ACATGT A^CATGT 1432 ACATGT BsrDI 7 GCAATG GCAATG(2/0) 1955 GCAATG DraIII 82 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 1219 CACTCAGTG EcoT14I 365 CCATGG C^C[AT][AT]GG 1881 CCATGG HaeIII 794 GGCC GG^CC 1563 GGCC HgaI 1267 GACGC GACGC(5/10) 2104 GACGC MaeI 4 CTAG C^TAG 941 CTAG MspA1I 34 CAGCTG C[AC]G^C[GT]G 1865 CCGCGG NcoI 365 CCATGG C^CATGG 1881 CCATGG NheI 3 GCTAGC G^CTAGC 940 GCTAGC PstI 37 CTGCAG CTGCA^G 1812 CTGCAG SfcI 37 CTGCAG C^T[GA][CT]AG 1812 CTGCAG StyI 365 CCATGG C^C[AT][AT]GG 1881 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AcsI AfaI AluI ApoI Asp700I BanI BanII BbvI BmyI BpmI BsaI BsaJI BsmAI Bsp1286I BsrI BstF5I Cac8I DdeI DpnII DsaI Fnu4HI FokI HindIII HinfI HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MslI MwoI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI SfaNI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI