(Linear) (Six-base) MAPSORT of: KIEE3522.seq Check: 8716 from: 1 to: 2136 KIEE3522.orf With 182 enzymes: SgfI * December 27, 2011 16:35 .. AccI GT'mk_AC Cuts at: 0 439 664 2136 Size: 439 225 1472 Fragments arranged by size: 1472 439 225 Acc65I G'GTAC_C Cuts at: 0 1327 2136 Size: 1327 809 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 304 847 1601 1952 2136 Size: 304 543 754 351 184 Fragments arranged by size: 754 543 351 304 184 AflIII A'CryG_T Cuts at: 0 24 1432 2136 Size: 24 1408 704 Fragments arranged by size: 1408 704 24 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1928 2136 Size: 1928 208 AlwNI CAG_nnn'CTG Cuts at: 0 1579 2136 Size: 1579 557 ApaLI G'TGCA_C Cuts at: 0 1977 2136 Size: 1977 159 ApoI r'AATT_y Cuts at: 0 828 852 1276 1567 2136 Size: 828 24 424 291 569 Fragments arranged by size: 828 569 424 291 24 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 974 1007 2136 Size: 974 33 1129 Fragments arranged by size: 1129 974 33 BanI G'GyrC_C Cuts at: 0 403 693 1327 2136 Size: 403 290 634 809 Fragments arranged by size: 809 634 403 290 BanII G_rGCy'C Cuts at: 0 656 1805 2075 2136 Size: 656 1149 270 61 Fragments arranged by size: 1149 656 270 61 BbsI GAAGACnn'nnnn_ Cuts at: 0 955 2108 2136 Size: 955 1153 28 Fragments arranged by size: 1153 955 28 BciVI GTATCCnnnnn_n' Cuts at: 0 6 458 2136 Size: 6 452 1678 Fragments arranged by size: 1678 452 6 BglII A'GATC_T Cuts at: 0 955 2136 Size: 955 1181 Bme1580I G_kGCm'C Cuts at: 0 408 1907 1981 2136 Size: 408 1499 74 155 Fragments arranged by size: 1499 408 155 74 BmtI G_CTAG'C Cuts at: 0 7 944 2136 Size: 7 937 1192 Fragments arranged by size: 1192 937 7 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 296 1023 2051 2136 Size: 296 727 1028 85 Fragments arranged by size: 1028 727 296 85 Bpu10I CC'TnA_GC Cuts at: 0 124 1271 2131 2136 Size: 124 1147 860 5 Fragments arranged by size: 1147 860 124 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 32 157 1988 2136 Size: 32 125 1831 148 Fragments arranged by size: 1831 148 125 32 BsaI GGTCTCn'nnnn_ Cuts at: 0 272 1203 1813 2136 Size: 272 931 610 323 Fragments arranged by size: 931 610 323 272 BsaAI yAC'GTr Cuts at: 0 17 1605 2136 Size: 17 1588 531 Fragments arranged by size: 1588 531 17 BsaBI GATnn'nnATC Cuts at: 0 1896 2136 Size: 1896 240 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 768 2136 Size: 768 1368 BseYI C'CCAG_C Cuts at: 0 1535 2136 Size: 1535 601 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 318 1699 2136 Size: 318 1381 437 Fragments arranged by size: 1381 437 318 BsiEI CG_ry'CG Cuts at: 0 104 2136 Size: 104 2032 BsiHKAI G_wGCw'C Cuts at: 0 1981 2075 2136 Size: 1981 94 61 Fragments arranged by size: 1981 94 61 BsmI GAATG_Cn' Cuts at: 0 1849 2136 Size: 1849 287 Bsp1286I G_dGCh'C Cuts at: 0 408 656 1805 1907 1981 2075 2136 Size: 408 248 1149 102 74 94 61 Fragments arranged by size: 1149 408 248 102 94 74 61 BspHI T'CATG_A Cuts at: 0 1146 2136 Size: 1146 990 BsrDI GCAATG_nn' Cuts at: 0 14 1962 2136 Size: 14 1948 174 Fragments arranged by size: 1948 174 14 BstBI TT'CG_AA Cuts at: 0 486 2136 Size: 486 1650 BstYI r'GATC_y Cuts at: 0 955 2136 Size: 955 1181 BstZ17I GTA'TAC Cuts at: 0 440 2136 Size: 440 1696 Bsu36I CC'TnA_GG Cuts at: 0 1700 2136 Size: 1700 436 BtgI C'CryG_G Cuts at: 0 365 1865 1881 2136 Size: 365 1500 16 255 Fragments arranged by size: 1500 365 255 16 BtsI GCAGTG_nn' Cuts at: 0 330 1521 1910 1950 2136 Size: 330 1191 389 40 186 Fragments arranged by size: 1191 389 330 186 40 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1156 1191 2136 Size: 1156 35 945 Fragments arranged by size: 1156 945 35 DraI TTT'AAA Cuts at: 0 905 2136 Size: 905 1231 DraIII CAC_nnn'GTG Cuts at: 0 87 1224 2136 Size: 87 1137 912 Fragments arranged by size: 1137 912 87 DrdI GACnn_nn'nnGTC Cuts at: 0 1049 2136 Size: 1049 1087 EaeI y'GGCC_r Cuts at: 0 1562 2136 Size: 1562 574 EarI CTCTTCn'nnn_ Cuts at: 0 155 2136 Size: 155 1981 EcoICRI GAG'CTC Cuts at: 0 2073 2136 Size: 2073 63 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 296 304 847 1023 1601 1952 2051 2136 Size: 296 8 543 176 578 351 99 85 Fragments arranged by size: 578 543 351 296 176 99 85 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 1699 2136 Size: 1699 437 EcoRI G'AATT_C Cuts at: 0 852 2136 Size: 852 1284 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1438 1470 1703 1735 2076 2101 2108 2133 Size: 1438 32 233 32 341 25 7 25 Cuts at: 2133 2136 Size: 3 Fragments arranged by size: 1438 341 233 32 32 25 25 7 3 HincII GTy'rAC Cuts at: 0 665 2136 Size: 665 1471 HindIII A'AGCT_T Cuts at: 0 907 1583 2006 2136 Size: 907 676 423 130 Fragments arranged by size: 907 676 423 130 KpnI G_GTAC'C Cuts at: 0 1331 2136 Size: 1331 805 MscI TGG'CCA Cuts at: 0 1564 2136 Size: 1564 572 MslI CAynn'nnrTG Cuts at: 0 401 1955 2096 2136 Size: 401 1554 141 40 Fragments arranged by size: 1554 401 141 40 MspA1I CmG'CkG Cuts at: 0 36 1867 2136 Size: 36 1831 269 Fragments arranged by size: 1831 269 36 NcoI C'CATG_G Cuts at: 0 365 1881 2136 Size: 365 1516 255 Fragments arranged by size: 1516 365 255 NheI G'CTAG_C Cuts at: 0 3 940 2136 Size: 3 937 1196 Fragments arranged by size: 1196 937 3 NspI r_CATG'y Cuts at: 0 28 169 1436 1445 1977 2136 Size: 28 141 1267 9 532 159 Fragments arranged by size: 1267 532 159 141 28 9 PciI A'CATG_T Cuts at: 0 24 1432 2136 Size: 24 1408 704 Fragments arranged by size: 1408 704 24 PflMI CCAn_nnn'nTGG Cuts at: 0 927 2136 Size: 927 1209 PstI C_TGCA'G Cuts at: 0 41 1816 2136 Size: 41 1775 320 Fragments arranged by size: 1775 320 41 PvuII CAG'CTG Cuts at: 0 36 2136 Size: 36 2100 SacI G_AGCT'C Cuts at: 0 2075 2136 Size: 2075 61 SacII CC_GC'GG Cuts at: 0 1868 2136 Size: 1868 268 SalI G'TCGA_C Cuts at: 0 663 2136 Size: 663 1473 SapI GCTCTTCn'nnn_ Cuts at: 0 155 2136 Size: 155 1981 ScaI AGT'ACT Cuts at: 0 734 2136 Size: 734 1402 SfcI C'TryA_G Cuts at: 0 37 1812 2136 Size: 37 1775 324 Fragments arranged by size: 1775 324 37 SmlI C'TyrA_G Cuts at: 0 47 172 2003 2136 Size: 47 125 1831 133 Fragments arranged by size: 1831 133 125 47 SnaBI TAC'GTA Cuts at: 0 1605 2136 Size: 1605 531 SphI G_CATG'C Cuts at: 0 169 2136 Size: 169 1967 StyI C'CwwG_G Cuts at: 0 365 1881 2136 Size: 365 1516 255 Fragments arranged by size: 1516 365 255 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 118 522 2136 Size: 118 404 1614 Fragments arranged by size: 1614 404 118 TatI w'GTAC_w Cuts at: 0 732 1130 1656 2136 Size: 732 398 526 480 Fragments arranged by size: 732 526 480 398 Tth111I GACn'n_nGTC Cuts at: 0 105 2136 Size: 105 2031 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 142 2136 Size: 142 1994 XmnI GAAnn'nnTTC Cuts at: 0 536 1103 1638 2136 Size: 536 567 535 498 Fragments arranged by size: 567 536 535 498 Enzymes that do cut: AccI Acc65I AcuI AflIII AlfI AlwNI ApaLI ApoI BaeI BanI BanII BbsI BciVI BglII Bme1580I BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrDI BstBI BstYI BstZ17I Bsu36I BtgI BtsI CspCI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoRI Hin4I HincII HindIII KpnI MscI MslI MspA1I NcoI NheI NspI PciI PflMI PstI PvuII SacI SacII SalI SapI ScaI SfcI SmlI SnaBI SphI StyI TaqII TatI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflII AgeI AhdI AleI AloI ApaI AscI AseI AsiSI AvaI AvrII BamHI BbeI BbvCI BcgI BclI BfrBI BglI BlpI BmgBI BmrI BplI BsaHI BsaWI BsaXI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BtgZI ClaI EagI EciI EcoO109I EcoRV FalI FseI FspI FspAI HaeII HpaI KasI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SrfI SspI StuI SwaI TstI XbaI XhoI XmaI ZraI