Restriction map for
pFN21AB8135
##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AgeI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BamHI BbvCI BcgI
BciVI BclI BglII BlnI BlpI
Bpu1102I BsaBI BsaWI BseAI BsmBI
BspDI BspEI BspHI BspMI BssHII
BssSI Bst1107I BstBI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI Eco47III Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
KpnI MamI MfeI MluI MroI
MunI NaeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspI StuI SwaI TaqI TfiI
Tsp509I TthHB8I Van91I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 901 GACGTC GACGT^C
AflII 663 CTTAAG C^TTAAG
AflIII 174 ACATGT A^C[GA][CT]GT
AhdI 844 GACCGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1338 GTGCAC G^TGCAC
ApaLI 1338 GTGCAC G^TGCAC
AspEI 844 GACCGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1338 GTGCAC G[AT]GC[AT]^C
BalI 104 TGGCCA TGG^CCA
BanII 10 GAGCCC G[GA]GC[CT]^C
BbeI 31 GGCGCC GGCGC^C
BbrPI 1456 CACGTG CAC^GTG
BfaI 1162 CTAG C^TAG
BfrI 663 CTTAAG C^TTAAG
BglI 775 GCCACCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1129 CTGGAG CTGGAG(16/14)
BsaAI 1456 CACGTG [CT]AC^GT[GA]
BsaI 271 GAGACC GGTCTC(1/5)
BseRI 323 CTCCTC GAGGAG(10/8)
BsgI 937 GTGCAG GTGCAG(16/14)
BsiHKAI 1338 GTGCAC G[AT]GC[AT]^C
BsiWI 408 CGTACG C^GTACG
BsmI 560 GCATTC GAATGC(1/-1)
Bsp1407I 437 TGTACA T^GTACA
BspLU11I 174 ACATGT A^CATGT
BsrBI 1288 GAGCGG CCGCTC(-3/-3)
BsrDI 764 CATTGC GCAATG(2/0)
BsrFI 1342 ACCGGC [GA]^CCGG[CT]
BsrGI 437 TGTACA T^GTACA
BstAPI 1340 GCACCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 1342 ACCGGC [GA]^CCGG[CT]
Eam1105I 844 GACCGAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 591 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 521 CCATGG C^C[AT][AT]GG
HaeII 31 GGCGCC [GA]GCGC^[CT]
HpaI 1309 GTTAAC GTT^AAC
KasI 31 GGCGCC G^GCGCC
MaeI 1162 CTAG C^TAG
MluNI 104 TGGCCA TGG^CCA
MscI 104 TGGCCA TGG^CCA
NarI 31 GGCGCC GG^CGCC
NcoI 521 CCATGG C^CATGG
NdeI 1410 CATATG CA^TATG
PmaCI 1456 CACGTG CAC^GTG
PmlI 1456 CACGTG CAC^GTG
PstI 122 CTGCAG CTGCA^G
SapI 530 GAAGAGC GCTCTTC(1/4)
SfcI 122 CTGCAG C^T[GA][CT]AG
SfoI 31 GGCGCC GGC^GCC
SmaI 13 CCCGGG CCC^GGG
SmlI 663 CTTAAG C^T[CT][GA]AG
SphI 1230 GCATGC GCATG^C
SspBI 437 TGTACA T^GTACA
StyI 521 CCATGG C^C[AT][AT]GG
XmaCI 13 CCCGGG C^CCGGG
XmaI 13 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 31 GGCGCC G[GA]^CG[CT]C
901 GACGTC
AspI 127 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1447 GACGAAGTC
BsaHI 31 GGCGCC G[GA]^CG[CT]C
901 GACGTC
BsiEI 462 CGGCCG CG[GA][CT]^CG
1317 CGGCCG
BsmAI 271 GAGAC GTCTC(1/5)
321 GTCTC
BstEII 87 GGTGACC G^GT[ACGT]ACC
1482 GGTGACC
BstPI 87 GGTGACC G^GT[ACGT]ACC
1482 GGTGACC
BstXI 140 CCACGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
249 CCACACAGATGG
BstYI 605 GGATCT [GA]^GATC[CT]
1271 AGATCC
EagI 462 CGGCCG C^GGCCG
1317 CGGCCG
EarI 530 GAAGAG CTCTTC(1/4)
1165 GAAGAG
EclXI 462 CGGCCG C^GGCCG
1317 CGGCCG
Eco52I 462 CGGCCG C^GGCCG
1317 CGGCCG
EcoO65I 87 GGTGACC G^GT[ACGT]ACC
1482 GGTGACC
FauI 1111 CCCGC CCCGC(4/6)
571 GCGGG
Hin1I 31 GGCGCC G[GA]^CG[CT]C
901 GACGTC
Ksp632I 530 GAAGAG CTCTTC(1/4)
1165 GAAGAG
KspI 779 CCGCGG CCGC^GG
1144 CCGCGG
MflI 605 GGATCT [GA]^GATC[CT]
1271 AGATCC
NspI 174 ACATGT [GA]CATG^[CT]
1230 GCATGC
PflFI 127 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1447 GACGAAGTC
PpuMI 312 AGGTCCT [GA]G^G[AT]CC[CT]
377 GGGACCC
SacII 779 CCGCGG CCGC^GG
1144 CCGCGG
Tth111I 127 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
1447 GACGAAGTC
XcmI 92 CCACAGTGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
348 CCAAGAAAAAGGTGG
XhoII 605 GGATCT [GA]^GATC[CT]
1271 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AvaI AvaII BanI BbsI
BbvI BcnI BmyI BpuAI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI Tru9I TseI Tsp45I TspRI