(Circular) (Six-base) MAPSORT of: pFN21AB8135.seq Check: 2936 from: 1 to: 6196 pFN21AB8135 6196 bp With 182 enzymes: SgfI * February 27, 2009 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2910 4006 278 Size: 53 83 186 936 1374 1096 2468 Fragments arranged by size: 2468 1374 1096 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3539 1937 Size: 1602 4594 Acc65I G'GTAC_C Cuts at: 3517 3517 Size: 6196 AclI AA'CG_TT Cuts at: 1563 4323 4696 1563 Size: 2760 373 3063 Fragments arranged by size: 3063 2760 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2189 3230 4259 5643 5911 1632 Size: 557 1041 1029 1384 268 1917 Fragments arranged by size: 1917 1384 1041 1029 557 268 AflII C'TTAA_G Cuts at: 820 1017 2668 820 Size: 197 1651 4348 Fragments arranged by size: 4348 1651 197 AflIII A'CryG_T Cuts at: 1203 2179 5095 6072 1203 Size: 976 2916 977 1327 Fragments arranged by size: 2916 1327 977 976 AgeI A'CCGG_T Cuts at: 5027 5027 Size: 6196 AhdI GACnn_n'nnGTC Cuts at: 2854 4925 2854 Size: 2071 4125 AleI CACnn'nnGTG Cuts at: 1166 2150 1166 Size: 984 5212 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6196 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2150 2182 2150 Size: 32 6164 AlwNI CAG_nnn'CTG Cuts at: 3026 3208 3434 3939 5511 3026 Size: 182 226 505 1572 3711 Fragments arranged by size: 3711 1572 505 226 182 ApaLI G'TGCA_C Cuts at: 3343 4253 5409 3343 Size: 910 1156 4130 Fragments arranged by size: 4130 1156 910 ApoI r'AATT_y Cuts at: 1493 3505 3731 3883 5992 1493 Size: 2012 226 152 2109 1697 Fragments arranged by size: 2109 2012 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4750 160 Size: 872 3718 1606 Fragments arranged by size: 3718 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 AvaI C'yCGr_G Cuts at: 1870 1943 2018 2812 2830 3521 1870 Size: 73 75 794 18 691 4545 Fragments arranged by size: 4545 794 691 75 73 18 BamHI G'GATC_C Cuts at: 3526 3893 3526 Size: 367 5829 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2036 2418 3109 3517 4972 Size: 325 217 627 249 382 691 408 1455 Cuts at: 4972 618 Size: 1842 Fragments arranged by size: 1842 1455 691 627 408 382 325 249 217 BanII G_rGCy'C Cuts at: 1391 2019 3515 1391 Size: 628 1496 4072 Fragments arranged by size: 4072 1496 628 BbeI G_GCGC'C Cuts at: 2040 2040 Size: 6196 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2131 2902 3218 928 Size: 971 232 771 316 3906 Fragments arranged by size: 3906 971 771 316 232 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4387 4421 1903 Size: 34 2450 34 3678 Fragments arranged by size: 3678 2450 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4088 5298 4088 Size: 1210 4986 BclI T'GATC_A Cuts at: 1548 1548 Size: 6196 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2786 4807 Size: 107 122 71 631 605 337 777 2021 Cuts at: 4807 136 Size: 1525 Fragments arranged by size: 2021 1525 777 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6191 6191 Size: 6196 BlpI GC'TnA_GC Cuts at: 3613 3613 Size: 6196 Bme1580I G_kGCm'C Cuts at: 1790 2025 2839 3347 4257 5413 1790 Size: 235 814 508 910 1156 2573 Fragments arranged by size: 2573 1156 910 814 508 235 BmgBI CAC'GTC Cuts at: 1318 2148 1318 Size: 830 5366 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2389 2672 3927 Size: 416 53 83 399 24 972 283 1255 Cuts at: 3927 4885 442 Size: 958 1753 Fragments arranged by size: 1753 1255 972 958 416 399 283 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6196 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3155 4856 1239 Size: 1916 1701 2579 Fragments arranged by size: 2579 1916 1701 Bpu10I CC'TnA_GC Cuts at: 2461 2970 2461 Size: 509 5687 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4321 5186 5484 4321 Size: 865 298 5033 Fragments arranged by size: 5033 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2270 4859 882 Size: 617 771 2589 2219 Fragments arranged by size: 2589 2219 771 617 BsaAI yAC'GTr Cuts at: 493 1204 3463 3987 493 Size: 711 2259 524 2702 Fragments arranged by size: 2702 2259 711 524 BsaBI GATnn'nnATC Cuts at: 6190 6190 Size: 6196 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2037 2907 4003 4385 Size: 53 83 186 936 504 870 1096 382 Cuts at: 4385 5020 275 Size: 635 1451 Fragments arranged by size: 1451 1096 936 870 635 504 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4629 5027 5301 5448 5859 4629 Size: 398 274 147 411 4966 Fragments arranged by size: 4966 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2486 2516 3356 3386 1178 Size: 30 165 30 1083 30 840 30 3988 Fragments arranged by size: 3988 1083 840 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2319 1186 Size: 1133 5063 BseYI C'CCAG_C Cuts at: 5399 5895 5399 Size: 496 5700 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2963 1163 Size: 573 1227 4396 Fragments arranged by size: 4396 1227 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2470 3325 3646 4407 5435 1540 Size: 275 186 469 855 321 761 1028 2301 Fragments arranged by size: 2301 1028 855 761 469 321 275 186 BsiHKAI G_wGCw'C Cuts at: 3347 4257 4342 5413 3347 Size: 910 85 1071 4130 Fragments arranged by size: 4130 1071 910 85 BsiWI C'GTAC_G Cuts at: 2413 2413 Size: 6196 BsmI GAATG_Cn' Cuts at: 2565 3707 3800 2565 Size: 1142 93 4961 Fragments arranged by size: 4961 1142 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2019 2025 2839 3347 3515 4257 4342 Size: 399 229 6 814 508 168 742 85 Cuts at: 4342 5413 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 814 742 508 399 229 168 85 6 BspEI T'CCGG_A Cuts at: 5859 5859 Size: 6196 BspHI T'CATG_A Cuts at: 4085 5815 4085 Size: 1730 4466 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3551 844 Size: 2707 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3295 4083 1394 Size: 429 1472 788 3507 Fragments arranged by size: 3507 1472 788 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2768 4691 4865 65 Size: 1183 1520 1923 174 1396 Fragments arranged by size: 1923 1520 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3347 4840 5027 1700 Size: 110 1537 1493 187 2869 Fragments arranged by size: 2869 1537 1493 187 110 BsrGI T'GTAC_A Cuts at: 96 2442 96 Size: 2346 3850 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6196 BssSI C'ACGA_G Cuts at: 4256 5268 4256 Size: 1012 5184 BstAPI GCAn_nnn'nTGC Cuts at: 794 3351 794 Size: 2557 3639 BstBI TT'CG_AA Cuts at: 5000 5000 Size: 6196 BstEII G'GTnAC_C Cuts at: 1184 2092 3487 1184 Size: 908 1395 3893 Fragments arranged by size: 3893 1395 908 BstXI CCAn_nnnn'nTGG Cuts at: 2152 2261 2152 Size: 109 6087 BstYI r'GATC_y Cuts at: 1972 2610 3276 3526 3893 4278 4295 5747 5833 Size: 638 666 250 367 385 17 1452 86 Cuts at: 5833 5845 6191 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 666 638 385 367 346 250 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2526 2784 2859 3149 3492 513 Size: 552 939 522 258 75 290 343 3217 Fragments arranged by size: 3217 939 552 522 343 290 258 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5457 Fragments arranged by size: 5457 683 56 BtsI GCAGTG_nn' Cuts at: 2597 2871 3712 4506 4526 2597 Size: 274 841 794 20 4267 Fragments arranged by size: 4267 841 794 274 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6196 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2500 2535 529 Size: 35 1936 35 4190 Fragments arranged by size: 4190 1936 35 35 DraI TTT'AAA Cuts at: 3500 3500 Size: 6196 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5203 809 Size: 507 3887 1802 Fragments arranged by size: 3887 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2109 2350 2467 Size: 54 1425 50 275 18 279 241 117 Cuts at: 2467 3322 3421 3643 4532 6161 8 Size: 855 99 222 889 1629 43 Fragments arranged by size: 1629 1425 889 855 279 275 241 222 117 99 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2467 3322 3643 1537 Size: 275 655 855 321 4090 Fragments arranged by size: 4090 855 655 321 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2530 3165 4126 1353 Size: 1177 635 961 3423 Fragments arranged by size: 3423 1177 961 635 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2027 4783 5157 5303 387 Size: 1064 138 438 2756 374 146 1280 Fragments arranged by size: 2756 1280 1064 438 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 3155 3230 4259 4856 5643 5911 Size: 393 557 966 75 1029 597 787 268 Cuts at: 5911 1239 Size: 1524 Fragments arranged by size: 1524 1029 966 787 597 557 393 268 75 EcoNI CCTnn'n_nnAGG Cuts at: 2600 2600 Size: 6196 EcoO109I rG'GnC_Cy Cuts at: 2044 2318 2383 2044 Size: 274 65 5857 Fragments arranged by size: 5857 274 65 EcoRI G'AATT_C Cuts at: 3505 3883 3505 Size: 378 5818 FspI TGC'GCA Cuts at: 4702 4702 Size: 6196 HaeII r_GCGC'y Cuts at: 1678 2040 5343 1678 Size: 362 3303 2531 Fragments arranged by size: 3303 2531 362 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2466 2498 2840 2872 4837 Size: 32 135 32 894 32 342 32 1965 Cuts at: 4837 4869 4911 4943 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1965 894 342 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3060 3181 3316 3364 3540 3786 Size: 988 281 1122 121 135 48 176 246 Cuts at: 3786 669 Size: 3079 Fragments arranged by size: 3079 1122 988 281 246 176 135 121 48 HindIII A'AGCT_T Cuts at: 748 748 Size: 6196 HpaI GTT'AAC Cuts at: 3316 3786 3316 Size: 470 5726 KasI G'GCGC_C Cuts at: 2036 2036 Size: 6196 KpnI G_GTAC'C Cuts at: 3521 3521 Size: 6196 MfeI C'AATT_G Cuts at: 3795 3795 Size: 6196 MluI A'CGCG_T Cuts at: 6072 6072 Size: 6196 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2357 5310 5494 1305 Size: 1052 2953 184 2007 Fragments arranged by size: 2953 2007 1052 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2111 10 Size: 54 1425 343 279 4095 Fragments arranged by size: 4095 1425 343 279 54 MslI CAynn'nnrTG Cuts at: 518 1166 2030 2150 2259 4154 4513 4672 518 Size: 648 864 120 109 1895 359 159 2042 Fragments arranged by size: 2042 1895 864 648 359 159 120 109 MspA1I CmG'CkG Cuts at: 1606 2072 2433 2786 3000 3151 3208 3434 3612 Size: 466 361 353 214 151 57 226 178 Cuts at: 3612 4289 5437 5682 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 466 361 353 245 226 214 178 151 57 NarI GG'CG_CC Cuts at: 2037 2037 Size: 6196 NcoI C'CATG_G Cuts at: 513 1065 2004 2526 513 Size: 552 939 522 4183 Fragments arranged by size: 4183 939 552 522 NdeI CA'TA_TG Cuts at: 387 3416 387 Size: 3029 3167 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6196 NotI GC'GGCC_GC Cuts at: 3643 3643 Size: 6196 NspI r_CATG'y Cuts at: 1132 1229 2183 3239 3554 5099 1132 Size: 97 954 1056 315 1545 2229 Fragments arranged by size: 2229 1545 1056 954 315 97 PasI CC'CwG_GG Cuts at: 1348 2899 1348 Size: 1551 4645 PciI A'CATG_T Cuts at: 2179 5095 2179 Size: 2916 3280 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6196 PfoI T'CCnGG_A Cuts at: 2601 2601 Size: 6196 PmeI GTTT'AAAC Cuts at: 3500 3500 Size: 6196 PmlI CAC'GTG Cuts at: 3463 3463 Size: 6196 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4265 4297 5778 5810 4265 Size: 32 1481 32 4651 Fragments arranged by size: 4651 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2318 2383 2318 Size: 65 6131 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6196 PsiI TTA'TAA Cuts at: 3766 3766 Size: 6196 PstI C_TGCA'G Cuts at: 830 2131 3548 830 Size: 1301 1417 3478 Fragments arranged by size: 3478 1417 1301 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6196 PvuII CAG'CTG Cuts at: 3000 3208 3434 3000 Size: 208 226 5762 Fragments arranged by size: 5762 226 208 SacII CC_GC'GG Cuts at: 2787 3152 2787 Size: 365 5831 SalI G'TCGA_C Cuts at: 1936 3538 1936 Size: 1602 4594 SanDI GG'GwC_CC Cuts at: 2383 2383 Size: 6196 SapI GCTCTTCn'nnn_ Cuts at: 2530 2530 Size: 6196 SbfI CC_TGCA'GG Cuts at: 3548 3548 Size: 6196 ScaI AGT'ACT Cuts at: 4444 6156 4444 Size: 1712 4484 SfcI C'TryA_G Cuts at: 826 1045 2127 3544 4679 5360 5551 826 Size: 219 1082 1417 1135 681 191 1471 Fragments arranged by size: 1471 1417 1135 1082 681 219 191 SfoI GGC'GCC Cuts at: 2038 2038 Size: 6196 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6196 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6196 SmaI CCC'GGG Cuts at: 1872 2020 3523 1872 Size: 148 1503 4545 Fragments arranged by size: 4545 1503 148 SmlI C'TyrA_G Cuts at: 820 1017 1943 2668 4300 5201 5463 820 Size: 197 926 725 1632 901 262 1553 Fragments arranged by size: 1632 1553 926 901 725 262 197 SnaBI TAC'GTA Cuts at: 493 3987 493 Size: 3494 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6196 SphI G_CATG'C Cuts at: 1132 3239 3554 1132 Size: 2107 315 3774 Fragments arranged by size: 3774 2107 315 SrfI GCCC'GGGC Cuts at: 2020 2020 Size: 6196 SspI AAT'ATT Cuts at: 5 52 4120 5 Size: 47 4068 2081 Fragments arranged by size: 4068 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6196 StyI C'CwwG_G Cuts at: 513 1065 2004 2526 3635 513 Size: 552 939 522 1109 3074 Fragments arranged by size: 3074 1109 939 552 522 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2865 4223 4240 4393 4578 5023 325 Size: 1255 1285 1358 17 153 185 445 1498 Fragments arranged by size: 1498 1358 1285 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2442 4442 6154 Size: 274 80 33 51 1443 465 2000 1712 Cuts at: 6154 96 Size: 138 Fragments arranged by size: 2000 1712 1443 465 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2302 2334 2758 2790 6156 6188 2302 Size: 32 424 32 3366 32 2310 Fragments arranged by size: 3366 2310 424 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2135 3455 1316 Size: 819 1320 4057 Fragments arranged by size: 4057 1320 819 XbaI T'CTAG_A Cuts at: 3532 3532 Size: 6196 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2104 2360 2104 Size: 256 5940 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6196 XmaI C'CCGG_G Cuts at: 1870 2018 3521 1870 Size: 148 1503 4545 Fragments arranged by size: 4545 1503 148 XmnI GAAnn'nnTTC Cuts at: 4325 4325 Size: 6196 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2908 4004 276 Size: 53 83 186 936 1374 1096 2468 Fragments arranged by size: 2468 1374 1096 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SexAI SfiI SwaI