Restriction map for
pFN21AB5402
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AvrII BalI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BpmI BsaBI BseAI BsiWI BsmBI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV FbaI FseI
HindIII HpaI KpnI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NcoI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflMI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SexAI SfiI
SfuI SmlI SnaBI SpeI SphI
SspBI SspI SwaI Tru9I Tsp509I
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 364 GTCTAC GT^[AC][GT]AC
AfaI 936 GTAC GT^AC
AgeI 1086 ACCGGT A^CCGGT
ApaI 161 GGGCCC GGGCC^C
Asp700I 541 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 748 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 164 CCCGGG C^[CT]CG[GA]G
AviII 32 TGCGCA TGC^GCA
BamHI 680 GGATCC G^GATCC
BbeI 23 GGCGCC GGCGC^C
BlpI 1116 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1116 GCTCAGC GC^T[ACGT]AGC
BsaAI 190 TACGTG [CT]AC^GT[GA]
BsaI 897 GGTCTC GGTCTC(1/5)
BsaWI 1086 ACCGGT [AT]^CCGG[AT]
BsiEI 251 CGGCCG CG[GA][CT]^CG
BsoBI 164 CCCGGG C^[CT]CG[GA]G
BspMI 605 ACCTGC ACCTGC(4/8)
BstAPI 288 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 815 CCATCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1116 GCTCAGC GC^T[ACGT]AGC
DraII 773 AGGTCCT [GA]G^G[ACGT]CC[CT]
EagI 251 CGGCCG C^GGCCG
EarI 1005 CTCTTC CTCTTC(1/4)
EclXI 251 CGGCCG C^GGCCG
Eco52I 251 CGGCCG C^GGCCG
EcoO109I 773 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 578 ATGCAT ATGCA^T
FspI 32 TGCGCA TGC^GCA
HgaI 245 GCGTC GACGC(5/10)
HincII 133 GTCAAC GT[CT]^[GA]AC
HindII 133 GTCAAC GT[CT]^[GA]AC
HinfI 940 GAATC G^A[ACGT]TC
KasI 23 GGCGCC G^GCGCC
Ksp632I 1005 CTCTTC CTCTTC(1/4)
KspI 358 CCGCGG CCGC^GG
MaeIII 7 GTCAC ^GT[ACGT]AC
NarI 23 GGCGCC GG^CGCC
NsiI 578 ATGCAT ATGCA^T
NspI 1043 ACATGC [GA]CATG^[CT]
PflFI 748 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1086 ACCGGT A^CCGGT
PpuMI 773 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 28 CAGCTG CAG^CTG
RsaI 936 GTAC GT^AC
SacI 1114 GAGCTC GAGCT^C
SacII 358 CCGCGG CCGC^GG
SapI 1004 GCTCTTC GCTCTTC(1/4)
SbfI 225 CCTGCAGG CCTGCA^GG
SfoI 23 GGCGCC GGC^GCC
SgrAI 1085 CACCGGTG C[GA]^CCGG[CT]G
SmaI 164 CCCGGG CCC^GGG
Sse8387I 225 CCTGCAGG CCTGCA^GG
TfiI 940 GAATC G^A[AT]TC
Tsp45I 7 GTCAC ^GT[GC]AC
Tth111I 748 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 164 CCCGGG C^CCGGG
XmaI 164 CCCGGG C^CCGGG
XmnI 541 GAAATCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 23 GGCGCC G[GA]^CG[CT]C
244 GGCGTC
AspHI 73 GTGCTC G[AT]GC[AT]^C
1114 GAGCTC
BbsI 691 GAAGAC GAAGAC(2/6)
837 GAAGAC
BcnI 164 CCCGG CC^[GC]GG
165 CCGGG
BpuAI 691 GAAGAC GAAGAC(2/6)
837 GAAGAC
BsaHI 23 GGCGCC G[GA]^CG[CT]C
244 GGCGTC
BsgI 607 CTGCAC GTGCAG(16/14)
1059 CTGCAC
BsiHKAI 73 GTGCTC G[AT]GC[AT]^C
1114 GAGCTC
DsaI 254 CCGTGG C^C[GA][CT]GG
358 CCGCGG
EaeI 251 CGGCCG [CT]^GGCC[GA]
1139 CGGCCA
EcoNI 225 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
855 CCTGAAGGAGG
EcoT14I 561 CCTTGG C^C[AT][AT]GG
987 CCAAGG
HaeII 23 GGCGCC [GA]GCGC^[CT]
307 AGCGCC
Hin1I 23 GGCGCC G[GA]^CG[CT]C
244 GGCGTC
HphI 326 TCACC GGTGA(8/7)
808 TCACC
MslI 581 CATCCAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
816 CATCTGCATG
MspA1I 28 CAGCTG C[AC]G^C[GT]G
358 CCGCGG
NaeI 83 GCCGGC GCC^GGC
352 GCCGGC
NciI 164 CCCGG CC^[GC]GG
165 CCGGG
NgoMIV 83 GCCGGC G^CCGGC
352 GCCGGC
PstI 226 CTGCAG CTGCA^G
931 CTGCAG
SfaNI 39 GATGC GCATC(5/9)
580 GCATC
StuI 650 AGGCCT AGG^CCT
863 AGGCCT
StyI 561 CCTTGG C^C[AT][AT]GG
987 CCAAGG
XcmI 783 CCACTGTGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
812 CCACCATCTGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
AvaII BanI BanII BbvI BmyI
BsaJI BseRI BsiYI BslI BsmAI
Bsp1286I BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeII MboI MboII MflI MnlI
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfcI TaqI TseI TspRI TthHB8I
XhoII