Restriction map for
pFN21AB5402
(Linear) (Six-base) MAPSORT of: KIBB5402.seq Check: 2814 from: 1 to: 1152
KIBB5402.orf
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
AccI GT'mk_AC
Cuts at: 0 365 1152
Size: 365 787
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 877 1152
Size: 877 275
AgeI A'CCGG_T
Cuts at: 0 1086 1152
Size: 1086 66
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 603 1152
Size: 603 549
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 789 821 1152
Size: 789 32 331
Fragments arranged by size:
789 331 32
AlwNI CAG_nnn'CTG
Cuts at: 0 294 930 1032 1152
Size: 294 636 102 120
Fragments arranged by size:
636 294 120 102
ApaI G_GGCC'C
Cuts at: 0 165 1152
Size: 165 987
AvaI C'yCGr_G
Cuts at: 0 164 1152
Size: 164 988
BamHI G'GATC_C
Cuts at: 0 680 1152
Size: 680 472
BanI G'GyrC_C
Cuts at: 0 23 1051 1083 1089 1152
Size: 23 1028 32 6 63
Fragments arranged by size:
1028 63 32 23 6
BanII G_rGCy'C
Cuts at: 0 165 468 1039 1118 1152
Size: 165 303 571 79 34
Fragments arranged by size:
571 303 165 79 34
BbeI G_GCGC'C
Cuts at: 0 27 1152
Size: 27 1125
BbsI GAAGACnn'nnnn_
Cuts at: 0 698 844 1152
Size: 698 146 308
Fragments arranged by size:
698 308 146
BfrBI ATG'CAT
Cuts at: 0 580 1152
Size: 580 572
BlpI GC'TnA_GC
Cuts at: 0 1117 1152
Size: 1117 35
Bme1580I G_kGCm'C
Cuts at: 0 165 1152
Size: 165 987
BmgBI CAC'GTC
Cuts at: 0 11 1152
Size: 11 1141
BmrI ACTGGGnnnn_n'
Cuts at: 0 529 1152
Size: 529 623
BsaI GGTCTCn'nnnn_
Cuts at: 0 903 1152
Size: 903 249
BsaAI yAC'GTr
Cuts at: 0 192 1152
Size: 192 960
BsaHI Gr'CG_yC
Cuts at: 0 24 245 1152
Size: 24 221 907
Fragments arranged by size:
907 221 24
BsaWI w'CCGG_w
Cuts at: 0 1086 1152
Size: 1086 66
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 700 730 758 788 1152
Size: 700 30 28 30 364
Fragments arranged by size:
700 364 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 316 900 903 1152
Size: 316 584 3 249
Fragments arranged by size:
584 316 249 3
BseYI C'CCAG_C
Cuts at: 0 793 971 1152
Size: 793 178 181
Fragments arranged by size:
793 181 178
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 1044 1152
Size: 592 452 108
Fragments arranged by size:
592 452 108
BsiEI CG_ry'CG
Cuts at: 0 254 1152
Size: 254 898
BsiHKAI G_wGCw'C
Cuts at: 0 77 1118 1152
Size: 77 1041 34
Fragments arranged by size:
1041 77 34
Bsp1286I G_dGCh'C
Cuts at: 0 77 165 468 1039 1118 1152
Size: 77 88 303 571 79 34
Fragments arranged by size:
571 303 88 79 77 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 614 1152
Size: 614 538
BsrFI r'CCGG_y
Cuts at: 0 83 352 1086 1152
Size: 83 269 734 66
Fragments arranged by size:
734 269 83 66
BssHII G'CGCG_C
Cuts at: 0 61 184 211 213 215 1152
Size: 61 123 27 2 2 937
Fragments arranged by size:
937 123 61 27 2 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 294 1152
Size: 294 858
BstXI CCAn_nnnn'nTGG
Cuts at: 0 822 1152
Size: 822 330
BstYI r'GATC_y
Cuts at: 0 680 797 947 1152
Size: 680 117 150 205
Fragments arranged by size:
680 205 150 117
BtgI C'CryG_G
Cuts at: 0 254 358 1152
Size: 254 104 794
Fragments arranged by size:
794 254 104
EaeI y'GGCC_r
Cuts at: 0 251 1139 1152
Size: 251 888 13
Fragments arranged by size:
888 251 13
EagI C'GGCC_G
Cuts at: 0 251 1152
Size: 251 901
EarI CTCTTCn'nnn_
Cuts at: 0 1011 1152
Size: 1011 141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 841 1152
Size: 841 311
EcoICRI GAG'CTC
Cuts at: 0 1116 1152
Size: 1116 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 877 1152
Size: 877 275
EcoNI CCTnn'n_nnAGG
Cuts at: 0 229 859 1152
Size: 229 630 293
Fragments arranged by size:
630 293 229
EcoO109I rG'GnC_Cy
Cuts at: 0 774 1152
Size: 774 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 882 1152
Size: 882 270
FspI TGC'GCA
Cuts at: 0 34 1152
Size: 34 1118
HaeII r_GCGC'y
Cuts at: 0 27 311 1152
Size: 27 284 841
Fragments arranged by size:
841 284 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 455 487 700 732 758 790 1152
Size: 455 32 213 32 26 32 362
Fragments arranged by size:
455 362 213 32 32 32 26
HincII GTy'rAC
Cuts at: 0 135 1152
Size: 135 1017
KasI G'GCGC_C
Cuts at: 0 23 1152
Size: 23 1129
MslI CAynn'nnrTG
Cuts at: 0 585 820 1152
Size: 585 235 332
Fragments arranged by size:
585 332 235
MspA1I CmG'CkG
Cuts at: 0 30 360 1152
Size: 30 330 792
Fragments arranged by size:
792 330 30
NaeI GCC'GGC
Cuts at: 0 85 354 1152
Size: 85 269 798
Fragments arranged by size:
798 269 85
NarI GG'CG_CC
Cuts at: 0 24 1152
Size: 24 1128
NgoMIV G'CCGG_C
Cuts at: 0 83 352 1152
Size: 83 269 800
Fragments arranged by size:
800 269 83
NsiI A_TGCA'T
Cuts at: 0 582 1152
Size: 582 570
NspI r_CATG'y
Cuts at: 0 1047 1152
Size: 1047 105
PpuMI rG'GwC_Cy
Cuts at: 0 774 1152
Size: 774 378
PspOMI G'GGCC_C
Cuts at: 0 161 1152
Size: 161 991
PstI C_TGCA'G
Cuts at: 0 230 935 1152
Size: 230 705 217
Fragments arranged by size:
705 230 217
PvuII CAG'CTG
Cuts at: 0 30 1152
Size: 30 1122
SacI G_AGCT'C
Cuts at: 0 1118 1152
Size: 1118 34
SacII CC_GC'GG
Cuts at: 0 361 1152
Size: 361 791
SapI GCTCTTCn'nnn_
Cuts at: 0 1011 1152
Size: 1011 141
SbfI CC_TGCA'GG
Cuts at: 0 230 1152
Size: 230 922
SfcI C'TryA_G
Cuts at: 0 226 507 931 1152
Size: 226 281 424 221
Fragments arranged by size:
424 281 226 221
SfoI GGC'GCC
Cuts at: 0 25 1152
Size: 25 1127
SgrAI Cr'CCGG_yG
Cuts at: 0 1086 1152
Size: 1086 66
SmaI CCC'GGG
Cuts at: 0 166 1152
Size: 166 986
StuI AGG'CCT
Cuts at: 0 652 865 1152
Size: 652 213 287
Fragments arranged by size:
652 287 213
StyI C'CwwG_G
Cuts at: 0 561 987 1152
Size: 561 426 165
Fragments arranged by size:
561 426 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 825 1152
Size: 825 327
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 476 508 1152
Size: 476 32 644
Fragments arranged by size:
644 476 32
Tth111I GACn'n_nGTC
Cuts at: 0 751 1152
Size: 751 401
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 790 819 1152
Size: 790 29 333
Fragments arranged by size:
790 333 29
XmaI C'CCGG_G
Cuts at: 0 164 1152
Size: 164 988
XmnI GAAnn'nnTTC
Cuts at: 0 545 1152
Size: 545 607
Enzymes that do cut:
AccI AcuI AgeI AlfI AloI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BfrBI BlpI Bme1580I
BmgBI BmrI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssHII
BstAPI BstXI BstYI BtgI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
HincII KasI MslI MspA1I NaeI NarI NgoMIV NsiI
NspI PpuMI PspOMI PstI PvuII SacI SacII SapI
SbfI SfcI SfoI SgrAI SmaI StuI StyI TaqII
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AhdI
AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbvCI BcgI BciVI BclI BglI BglII BmtI BplI
BpmI Bpu10I BpuEI BsaBI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrGI BssSI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EcoRI EcoRV FseI FspAI HindIII HpaI KpnI MfeI
MluI MmeI MscI NcoI NdeI NheI NotI NruI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI
ScaI SexAI SfiI SgfI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TatI XbaI XhoI ZraI