(Circular) (Six-base) MAPSORT of: pFN21AB6278.seq Check: 2941 from: 1 to: 7048 pFN21AB6278 7048 bp With 182 enzymes: SgfI * April 15, 2010 09:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3500 3500 Size: 7048 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4858 278 Size: 53 83 186 936 3322 2468 Fragments arranged by size: 3322 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3604 4391 1937 Size: 1667 787 4594 Fragments arranged by size: 4594 1667 787 Acc65I G'GTAC_C Cuts at: 3839 4369 3839 Size: 530 6518 AclI AA'CG_TT Cuts at: 1563 3398 5175 5548 1563 Size: 1835 1777 373 3063 Fragments arranged by size: 3063 1835 1777 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2417 2562 3084 3092 3159 3305 3696 5111 Size: 785 145 522 8 67 146 391 1415 Cuts at: 5111 6495 6763 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1415 1384 785 522 391 268 146 145 67 8 AflII C'TTAA_G Cuts at: 820 1017 3730 820 Size: 197 2713 4138 Fragments arranged by size: 4138 2713 197 AflIII A'CryG_T Cuts at: 1203 5947 6924 1203 Size: 4744 977 1327 Fragments arranged by size: 4744 1327 977 AgeI A'CCGG_T Cuts at: 5879 5879 Size: 7048 AhdI GACnn_n'nnGTC Cuts at: 5777 5777 Size: 7048 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7048 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4163 1184 Size: 2979 4069 AlwNI CAG_nnn'CTG Cuts at: 4328 4791 6363 4328 Size: 463 1572 5013 Fragments arranged by size: 5013 1572 463 ApaI G_GGCC'C Cuts at: 2266 2266 Size: 7048 ApaLI G'TGCA_C Cuts at: 5105 6261 5105 Size: 1156 5892 ApoI r'AATT_y Cuts at: 1493 2437 2613 3430 4043 4357 4583 4735 6844 Size: 944 176 817 613 314 226 152 2109 Cuts at: 6844 1493 Size: 1697 Fragments arranged by size: 2109 1697 944 817 613 314 226 176 152 AseI AT'TA_AT Cuts at: 160 1032 2874 2964 5602 160 Size: 872 1842 90 2638 1606 Fragments arranged by size: 2638 1842 1606 872 90 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7048 AvaI C'yCGr_G Cuts at: 1870 1943 2842 4373 1870 Size: 73 899 1531 4545 Fragments arranged by size: 4545 1531 899 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2787 2820 2787 Size: 33 7015 BamHI G'GATC_C Cuts at: 4378 4745 4378 Size: 367 6681 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3839 4369 5824 618 Size: 325 217 627 2052 530 1455 1842 Fragments arranged by size: 2052 1842 1455 627 530 325 217 BanII G_rGCy'C Cuts at: 1391 2266 3925 4367 1391 Size: 875 1659 442 4072 Fragments arranged by size: 4072 1659 875 442 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3911 928 Size: 971 2012 4065 Fragments arranged by size: 4065 2012 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5239 5273 1903 Size: 34 3302 34 3678 Fragments arranged by size: 3678 3302 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4940 6150 4940 Size: 1210 5838 BclI T'GATC_A Cuts at: 1548 1548 Size: 7048 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5659 136 Size: 107 122 71 631 605 3987 1525 Fragments arranged by size: 3987 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3103 7043 3103 Size: 3940 3108 BlpI GC'TnA_GC Cuts at: 4465 4465 Size: 7048 Bme1580I G_kGCm'C Cuts at: 1790 2266 5109 6265 1790 Size: 476 2843 1156 2573 Fragments arranged by size: 2843 2573 1156 476 BmgBI CAC'GTC Cuts at: 1318 2056 1318 Size: 738 6310 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4779 5737 442 Size: 416 53 83 399 24 3362 958 1753 Fragments arranged by size: 3362 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7048 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3516 3963 5708 1239 Size: 2277 447 1745 2579 Fragments arranged by size: 2579 2277 1745 447 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5173 6038 6336 5173 Size: 865 298 5885 Fragments arranged by size: 5885 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5711 882 Size: 617 4212 2219 Fragments arranged by size: 4212 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4839 493 Size: 711 3635 2702 Fragments arranged by size: 3635 2702 711 BsaBI GATnn'nnATC Cuts at: 7042 7042 Size: 7048 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3473 4855 5237 5872 Size: 53 83 186 936 1940 1382 382 635 Cuts at: 5872 275 Size: 1451 Fragments arranged by size: 1940 1451 1382 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5481 5879 6153 6300 6711 5481 Size: 398 274 147 411 5818 Fragments arranged by size: 5818 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2324 2354 3514 3544 1178 Size: 30 165 30 921 30 1160 30 4682 Fragments arranged by size: 4682 1160 921 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2252 2255 2328 1186 Size: 1066 3 73 5906 Fragments arranged by size: 5906 1066 73 3 BseYI C'CCAG_C Cuts at: 6251 6747 6251 Size: 496 6552 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2286 1163 Size: 573 550 5925 Fragments arranged by size: 5925 573 550 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2250 4498 5259 6287 1540 Size: 275 186 249 2248 761 1028 2301 Fragments arranged by size: 2301 2248 1028 761 275 249 186 BsiHKAI G_wGCw'C Cuts at: 2320 3925 5109 5194 6265 2320 Size: 1605 1184 85 1071 3103 Fragments arranged by size: 3103 1605 1184 1071 85 BsmI GAATG_Cn' Cuts at: 3631 3937 4559 4652 3631 Size: 306 622 93 6027 Fragments arranged by size: 6027 622 306 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2266 2320 3925 4367 5109 5194 6265 Size: 399 476 54 1605 442 742 85 1071 Cuts at: 6265 1391 Size: 2174 Fragments arranged by size: 2174 1605 1071 742 476 442 399 85 54 BspEI T'CCGG_A Cuts at: 6711 6711 Size: 7048 BspHI T'CATG_A Cuts at: 4937 6667 4937 Size: 1730 5318 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2207 2542 3079 3500 4403 844 Size: 1363 335 537 421 903 3489 Fragments arranged by size: 3489 1363 903 537 421 335 BsrBI CCG'CTC Cuts at: 1394 1823 2012 2179 2947 4935 1394 Size: 429 189 167 768 1988 3507 Fragments arranged by size: 3507 1988 768 429 189 167 BsrDI GCAATG_nn' Cuts at: 65 1248 5543 5717 65 Size: 1183 4295 174 1396 Fragments arranged by size: 4295 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5692 5879 1700 Size: 110 3882 187 2869 Fragments arranged by size: 3882 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3187 96 Size: 3091 3957 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7048 BssSI C'ACGA_G Cuts at: 5108 6120 5108 Size: 1012 6036 BstAPI GCAn_nnn'nTGC Cuts at: 794 3433 794 Size: 2639 4409 BstBI TT'CG_AA Cuts at: 5852 5852 Size: 7048 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7048 BstXI CCAn_nnnn'nTGG Cuts at: 2328 2328 Size: 7048 BstYI r'GATC_y Cuts at: 1972 2529 3103 4378 4745 5130 5147 6599 6685 Size: 557 574 1275 367 385 17 1452 86 Cuts at: 6685 6697 7043 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1275 574 557 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3605 3605 Size: 7048 BtgI C'CryG_G Cuts at: 513 1065 2092 3268 513 Size: 552 1027 1176 4293 Fragments arranged by size: 4293 1176 1027 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2205 487 Size: 683 56 979 5330 Fragments arranged by size: 5330 979 683 56 BtsI GCAGTG_nn' Cuts at: 4564 5358 5378 4564 Size: 794 20 6234 Fragments arranged by size: 6234 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7048 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7013 DraI TTT'AAA Cuts at: 4352 4352 Size: 7048 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6055 809 Size: 507 4739 1802 Fragments arranged by size: 4739 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2089 2190 2247 Size: 54 1425 50 275 18 259 101 57 Cuts at: 2247 4495 5384 7013 8 Size: 2248 889 1629 43 Fragments arranged by size: 2248 1629 1425 889 275 259 101 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2247 4495 1537 Size: 275 435 2248 4090 Fragments arranged by size: 4090 2248 435 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2581 3013 3135 3672 3829 4978 1353 Size: 1228 432 122 537 157 1149 3423 Fragments arranged by size: 3423 1228 1149 537 432 157 122 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4255 5635 6009 6155 387 Size: 1064 138 2666 1380 374 146 1280 Fragments arranged by size: 2666 1380 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3923 3923 Size: 7048 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2417 2562 3084 3092 3159 3305 3516 Size: 393 785 145 522 8 67 146 211 Cuts at: 3516 3696 3963 5111 5708 6495 6763 1239 Size: 180 267 1148 597 787 268 1524 Fragments arranged by size: 1524 1148 787 785 597 522 393 268 267 211 180 146 145 67 8 EcoO109I rG'GnC_Cy Cuts at: 2262 2263 2262 Size: 1 7047 EcoRI G'AATT_C Cuts at: 4357 4735 4357 Size: 378 6670 EcoRV GAT'ATC Cuts at: 4285 4285 Size: 7048 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4304 4304 Size: 7048 FspI TGC'GCA Cuts at: 5554 5554 Size: 7048 HaeII r_GCGC'y Cuts at: 1678 6195 1678 Size: 4517 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2324 2356 3898 3910 3930 Size: 32 135 32 752 32 1542 12 20 Cuts at: 3930 3942 5689 5721 5763 5795 1373 Size: 12 1747 32 42 32 2626 Fragments arranged by size: 2626 1747 1542 752 135 42 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 4392 4638 669 Size: 988 281 2454 246 3079 Fragments arranged by size: 3079 2454 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7048 HpaI GTT'AAC Cuts at: 4638 4638 Size: 7048 KpnI G_GTAC'C Cuts at: 3843 4373 3843 Size: 530 6518 MfeI C'AATT_G Cuts at: 2724 4647 2724 Size: 1923 5125 MluI A'CGCG_T Cuts at: 6924 6924 Size: 7048 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6162 6346 1305 Size: 4857 184 2007 Fragments arranged by size: 4857 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5226 Fragments arranged by size: 5226 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4032 5006 5365 5524 518 Size: 648 2866 974 359 159 2042 Fragments arranged by size: 2866 2042 974 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2150 3331 4464 5141 6289 6534 1606 Size: 544 1181 1133 677 1148 245 2120 Fragments arranged by size: 2120 1181 1148 1133 677 544 245 NcoI C'CATG_G Cuts at: 513 1065 3268 513 Size: 552 2203 4293 Fragments arranged by size: 4293 2203 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7048 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7048 NotI GC'GGCC_GC Cuts at: 2247 4495 2247 Size: 2248 4800 NspI r_CATG'y Cuts at: 1132 1229 4406 5951 1132 Size: 97 3177 1545 2229 Fragments arranged by size: 3177 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7048 PciI A'CATG_T Cuts at: 5947 5947 Size: 7048 PflMI CCAn_nnn'nTGG Cuts at: 1108 2696 3887 1108 Size: 1588 1191 4269 Fragments arranged by size: 4269 1588 1191 PfoI T'CCnGG_A Cuts at: 3337 3616 3337 Size: 279 6769 PmeI GTTT'AAAC Cuts at: 4352 4352 Size: 7048 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5117 5149 6630 6662 5117 Size: 32 1481 32 5503 Fragments arranged by size: 5503 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7048 PsiI TTA'TAA Cuts at: 4618 4618 Size: 7048 PspOMI G'GGCC_C Cuts at: 2262 2262 Size: 7048 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4243 4275 4243 Size: 32 7016 PstI C_TGCA'G Cuts at: 830 2109 2204 2869 3643 4195 4400 830 Size: 1279 95 665 774 552 205 3478 Fragments arranged by size: 3478 1279 774 665 552 205 95 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7048 PvuII CAG'CTG Cuts at: 3331 3331 Size: 7048 SacI G_AGCT'C Cuts at: 3925 3925 Size: 7048 SalI G'TCGA_C Cuts at: 1936 4390 1936 Size: 2454 4594 SapI GCTCTTCn'nnn_ Cuts at: 2581 2581 Size: 7048 SbfI CC_TGCA'GG Cuts at: 4400 4400 Size: 7048 ScaI AGT'ACT Cuts at: 5296 7008 5296 Size: 1712 5336 SfcI C'TryA_G Cuts at: 826 1045 2105 2200 2865 3171 3588 3639 4191 Size: 219 1060 95 665 306 417 51 552 Cuts at: 4191 4396 5531 6212 6403 826 Size: 205 1135 681 191 1471 Fragments arranged by size: 1471 1135 1060 681 665 552 417 306 219 205 191 95 51 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7048 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7048 SmaI CCC'GGG Cuts at: 1872 4375 1872 Size: 2503 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3730 5152 6053 6315 820 Size: 197 926 1787 1422 901 262 1553 Fragments arranged by size: 1787 1553 1422 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4839 493 Size: 4346 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7048 SphI G_CATG'C Cuts at: 1132 4406 1132 Size: 3274 3774 SspI AAT'ATT Cuts at: 5 52 2457 2794 3160 4222 4972 5 Size: 47 2405 337 366 1062 750 2081 Fragments arranged by size: 2405 2081 1062 750 366 337 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7048 StyI C'CwwG_G Cuts at: 513 1065 2049 3268 4487 513 Size: 552 984 1219 1219 3074 Fragments arranged by size: 3074 1219 1219 984 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5075 5092 5245 5430 5875 325 Size: 1255 3495 17 153 185 445 1498 Fragments arranged by size: 3495 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3038 3187 3406 Size: 274 80 33 51 1443 1061 149 219 Cuts at: 3406 3736 3972 4142 5294 7006 96 Size: 330 236 170 1152 1712 138 Fragments arranged by size: 1712 1443 1152 1061 330 274 236 219 170 149 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3249 3281 3514 3546 3961 3993 7008 7040 3249 Size: 32 233 32 415 32 3015 32 3257 Fragments arranged by size: 3257 3015 415 233 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7048 XbaI T'CTAG_A Cuts at: 2824 4384 2824 Size: 1560 5488 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2540 2540 Size: 7048 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7048 XmaI C'CCGG_G Cuts at: 1870 4373 1870 Size: 2503 4545 XmnI GAAnn'nnTTC Cuts at: 2662 5177 2662 Size: 2515 4533 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4856 276 Size: 53 83 186 936 3322 2468 Fragments arranged by size: 3322 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI