(Circular) (Six-base) MAPSORT of: pFN21AB5980.seq Check: 3043 from: 1 to: 6406 pFN21AB5980 6406 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2575 2575 Size: 6406 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4216 278 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3749 1937 Size: 1812 4594 Acc65I G'GTAC_C Cuts at: 2238 3727 2238 Size: 1489 4917 AclI AA'CG_TT Cuts at: 1563 4533 4906 1563 Size: 2970 373 3063 Fragments arranged by size: 3063 2970 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2437 2940 4469 5853 6121 1632 Size: 805 503 1529 1384 268 1917 Fragments arranged by size: 1917 1529 1384 805 503 268 AfeI AGC'GCT Cuts at: 2312 2312 Size: 6406 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6209 AflIII A'CryG_T Cuts at: 1203 2223 5305 6282 1203 Size: 1020 3082 977 1327 Fragments arranged by size: 3082 1327 1020 977 AgeI A'CCGG_T Cuts at: 2119 2783 5237 2119 Size: 664 2454 3288 Fragments arranged by size: 3288 2454 664 AhdI GACnn_n'nnGTC Cuts at: 5135 5135 Size: 6406 AleI CACnn'nnGTG Cuts at: 1166 2120 1166 Size: 954 5452 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6406 AlwNI CAG_nnn'CTG Cuts at: 2134 2551 2590 2878 4149 5721 2134 Size: 417 39 288 1271 1572 2819 Fragments arranged by size: 2819 1572 1271 417 288 39 ApaI G_GGCC'C Cuts at: 2085 2085 Size: 6406 ApaLI G'TGCA_C Cuts at: 3251 4463 5619 3251 Size: 1212 1156 4038 Fragments arranged by size: 4038 1212 1156 ApoI r'AATT_y Cuts at: 1493 3715 3941 4093 6202 1493 Size: 2222 226 152 2109 1697 Fragments arranged by size: 2222 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4960 160 Size: 872 3928 1606 Fragments arranged by size: 3928 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 AvaI C'yCGr_G Cuts at: 1870 1943 2084 2092 2648 2969 3016 3501 3731 Size: 73 141 8 556 321 47 485 230 Cuts at: 3731 1870 Size: 4545 Fragments arranged by size: 4545 556 485 321 230 141 73 47 8 BamHI G'GATC_C Cuts at: 3736 4103 3736 Size: 367 6039 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 2238 2688 2728 2975 Size: 325 217 627 335 116 450 40 247 Cuts at: 2975 3161 3314 3727 5182 618 Size: 186 153 413 1455 1842 Fragments arranged by size: 1842 1455 627 450 413 335 325 247 217 186 153 116 40 BanII G_rGCy'C Cuts at: 1391 2085 2099 2153 3047 3195 3725 1391 Size: 694 14 54 894 148 530 4072 Fragments arranged by size: 4072 894 694 530 148 54 14 BbeI G_GCGC'C Cuts at: 2692 2732 2979 3318 2692 Size: 40 247 339 5780 Fragments arranged by size: 5780 339 247 40 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2178 3276 3417 928 Size: 971 279 1098 141 3917 Fragments arranged by size: 3917 1098 971 279 141 BbvCI CC'TCA_GC Cuts at: 2907 3035 2907 Size: 128 6278 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3139 3173 3346 3380 4597 4631 1903 Size: 34 1202 34 173 34 1217 34 3678 Fragments arranged by size: 3678 1217 1202 173 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4298 5508 4298 Size: 1210 5196 BclI T'GATC_A Cuts at: 1548 1548 Size: 6406 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2148 2891 2903 Size: 107 122 71 631 605 476 743 12 Cuts at: 2903 3359 5017 136 Size: 456 1658 1525 Fragments arranged by size: 1658 1525 743 631 605 476 456 122 107 71 12 BglII A'GATC_T Cuts at: 6401 6401 Size: 6406 BlpI GC'TnA_GC Cuts at: 3823 3823 Size: 6406 Bme1580I G_kGCm'C Cuts at: 1790 2085 2562 3255 4467 5623 1790 Size: 295 477 693 1212 1156 2573 Fragments arranged by size: 2573 1212 1156 693 477 295 BmgBI CAC'GTC Cuts at: 1318 2224 1318 Size: 906 5500 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2267 2403 3565 Size: 416 53 83 399 24 850 136 1162 Cuts at: 3565 4137 5095 442 Size: 572 958 1753 Fragments arranged by size: 1753 1162 958 850 572 416 399 136 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6406 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2516 2870 2900 3209 3364 3426 3668 5066 Size: 1277 354 30 309 155 62 242 1398 Cuts at: 5066 1239 Size: 2579 Fragments arranged by size: 2579 1398 1277 354 309 242 155 62 30 Bpu10I CC'TnA_GC Cuts at: 2907 3035 2907 Size: 128 6278 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2941 3533 4531 5396 5694 2941 Size: 592 998 865 298 3653 Fragments arranged by size: 3653 998 865 592 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2420 3119 5069 882 Size: 617 921 699 1950 2219 Fragments arranged by size: 2219 1950 921 699 617 BsaAI yAC'GTr Cuts at: 493 1204 2245 4197 493 Size: 711 1041 1952 2702 Fragments arranged by size: 2702 1952 1041 711 BsaBI GATnn'nnATC Cuts at: 3215 3221 6400 3215 Size: 6 3179 3221 Fragments arranged by size: 3221 3179 6 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2689 2729 2976 3315 Size: 53 83 186 936 1156 40 247 339 Cuts at: 3315 4213 4595 5230 275 Size: 898 382 635 1451 Fragments arranged by size: 1451 1156 936 898 635 382 339 247 186 83 53 40 BsaWI w'CCGG_w Cuts at: 2119 2783 3516 4839 5237 5511 5658 6069 2119 Size: 664 733 1323 398 274 147 411 2456 Fragments arranged by size: 2456 1323 733 664 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2466 2496 1178 Size: 30 165 30 1063 30 5088 Fragments arranged by size: 5088 1063 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2507 2867 3537 1186 Size: 1321 360 670 4055 Fragments arranged by size: 4055 1321 670 360 BseYI C'CCAG_C Cuts at: 2262 2292 2503 2995 3246 3472 5609 6105 2262 Size: 30 211 492 251 226 2137 496 2563 Fragments arranged by size: 2563 2137 496 492 251 226 211 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2576 1163 Size: 573 840 4993 Fragments arranged by size: 4993 840 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3028 3856 4617 5645 1540 Size: 275 186 1027 828 761 1028 2301 Fragments arranged by size: 2301 1028 1027 828 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3255 3350 4467 4552 5623 3255 Size: 95 1117 85 1071 4038 Fragments arranged by size: 4038 1117 1071 95 85 BsmI GAATG_Cn' Cuts at: 3400 3917 4010 3400 Size: 517 93 5796 Fragments arranged by size: 5796 517 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2085 2099 2153 2562 3047 3195 3255 Size: 399 295 14 54 409 485 148 60 Cuts at: 3255 3350 3725 4467 4552 5623 1391 Size: 95 375 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 485 409 399 375 295 148 95 85 60 54 14 BspEI T'CCGG_A Cuts at: 6069 6069 Size: 6406 BspHI T'CATG_A Cuts at: 4295 6025 4295 Size: 1730 4676 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2575 2877 2975 3761 844 Size: 1731 302 98 786 3489 Fragments arranged by size: 3489 1731 786 302 98 BsrBI CCG'CTC Cuts at: 1394 1823 2745 2857 4293 1394 Size: 429 922 112 1436 3507 Fragments arranged by size: 3507 1436 922 429 112 BsrDI GCAATG_nn' Cuts at: 65 1248 4901 5075 65 Size: 1183 3653 174 1396 Fragments arranged by size: 3653 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2119 2783 5050 5237 1700 Size: 110 309 664 2267 187 2869 Fragments arranged by size: 2869 2267 664 309 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6406 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6406 BssSI C'ACGA_G Cuts at: 4466 5478 4466 Size: 1012 5394 BstAPI GCAn_nnn'nTGC Cuts at: 794 2590 3243 3354 794 Size: 1796 653 111 3846 Fragments arranged by size: 3846 1796 653 111 BstBI TT'CG_AA Cuts at: 5210 5210 Size: 6406 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6406 BstYI r'GATC_y Cuts at: 1972 2457 3736 4103 4488 4505 5957 6043 6055 Size: 485 1279 367 385 17 1452 86 12 Cuts at: 6055 6401 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1279 485 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3055 3055 Size: 6406 BtgI C'CryG_G Cuts at: 513 1065 2706 2886 513 Size: 552 1641 180 4033 Fragments arranged by size: 4033 1641 552 180 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3421 487 Size: 683 56 2195 3472 Fragments arranged by size: 3472 2195 683 56 BtsI GCAGTG_nn' Cuts at: 2757 3922 4716 4736 2757 Size: 1165 794 20 4427 Fragments arranged by size: 4427 1165 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6406 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6371 DraI TTT'AAA Cuts at: 3710 3710 Size: 6406 DraIII CAC_nnn'GTG Cuts at: 2209 2248 2619 2712 3448 2209 Size: 39 371 93 736 5167 Fragments arranged by size: 5167 736 371 93 39 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5413 809 Size: 507 4097 1802 Fragments arranged by size: 4097 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2112 3025 3372 Size: 54 1425 50 275 18 282 913 347 Cuts at: 3372 3853 4742 6371 8 Size: 481 889 1629 43 Fragments arranged by size: 1629 1425 913 889 481 347 282 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3025 3853 1537 Size: 275 1213 828 4090 Fragments arranged by size: 4090 1213 828 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2330 2564 2959 3007 3080 4336 1353 Size: 977 234 395 48 73 1256 3423 Fragments arranged by size: 3423 1256 977 395 234 73 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4993 5367 5513 387 Size: 1064 138 3404 374 146 1280 Fragments arranged by size: 3404 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2437 2516 2870 2900 2940 3209 3364 Size: 393 805 79 354 30 40 269 155 Cuts at: 3364 3426 3668 4469 5066 5853 6121 1239 Size: 62 242 801 597 787 268 1524 Fragments arranged by size: 1524 805 801 787 597 393 354 269 268 242 155 79 62 40 30 EcoNI CCTnn'n_nnAGG Cuts at: 3566 3602 3566 Size: 36 6370 EcoO109I rG'GnC_Cy Cuts at: 2140 2548 2866 2987 3133 3559 2140 Size: 408 318 121 146 426 4987 Fragments arranged by size: 4987 426 408 318 146 121 EcoRI G'AATT_C Cuts at: 3715 4093 3715 Size: 378 6028 FspI TGC'GCA Cuts at: 2252 4912 2252 Size: 2660 3746 HaeII r_GCGC'y Cuts at: 1678 2013 2314 2692 2732 2979 3318 3624 5553 Size: 335 301 378 40 247 339 306 1929 Cuts at: 5553 1678 Size: 2531 Fragments arranged by size: 2531 1929 378 339 335 306 301 247 40 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5047 5079 5121 5153 1373 Size: 32 135 32 3475 32 42 32 2626 Fragments arranged by size: 3475 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3750 3996 669 Size: 988 281 1812 246 3079 Fragments arranged by size: 3079 1812 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6406 HpaI GTT'AAC Cuts at: 3996 3996 Size: 6406 KasI G'GCGC_C Cuts at: 2688 2728 2975 3314 2688 Size: 40 247 339 5780 Fragments arranged by size: 5780 339 247 40 KpnI G_GTAC'C Cuts at: 2242 3731 2242 Size: 1489 4917 MfeI C'AATT_G Cuts at: 4005 4005 Size: 6406 MluI A'CGCG_T Cuts at: 6282 6282 Size: 6406 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2178 2562 5520 5704 1305 Size: 873 384 2958 184 2007 Fragments arranged by size: 2958 2007 873 384 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2114 3374 10 Size: 54 1425 343 282 1260 3042 Fragments arranged by size: 3042 1425 1260 343 282 54 MslI CAynn'nnrTG Cuts at: 518 1166 2120 4364 4723 4882 518 Size: 648 954 2244 359 159 2042 Fragments arranged by size: 2244 2042 954 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2037 2046 2363 2940 3674 3822 4499 5647 Size: 431 9 317 577 734 148 677 1148 Cuts at: 5647 5892 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 734 677 577 431 317 245 148 9 NarI GG'CG_CC Cuts at: 2689 2729 2976 3315 2689 Size: 40 247 339 5780 Fragments arranged by size: 5780 339 247 40 NcoI C'CATG_G Cuts at: 513 1065 2886 513 Size: 552 1821 4033 Fragments arranged by size: 4033 1821 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6406 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6406 NotI GC'GGCC_GC Cuts at: 3853 3853 Size: 6406 NspI r_CATG'y Cuts at: 1132 1229 3764 5309 1132 Size: 97 2535 1545 2229 Fragments arranged by size: 2535 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6406 PciI A'CATG_T Cuts at: 5305 5305 Size: 6406 PflMI CCAn_nnn'nTGG Cuts at: 1108 2283 2712 1108 Size: 1175 429 4802 Fragments arranged by size: 4802 1175 429 PfoI T'CCnGG_A Cuts at: 2394 2394 Size: 6406 PmeI GTTT'AAAC Cuts at: 3710 3710 Size: 6406 PmlI CAC'GTG Cuts at: 2245 2245 Size: 6406 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4475 4507 5988 6020 4475 Size: 32 1481 32 4861 Fragments arranged by size: 4861 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2866 2987 2866 Size: 121 6285 PshAI GACnn'nnGTC Cuts at: 1613 2432 3417 1613 Size: 819 985 4602 Fragments arranged by size: 4602 985 819 PsiI TTA'TAA Cuts at: 3976 3976 Size: 6406 PspOMI G'GGCC_C Cuts at: 2081 2081 Size: 6406 PstI C_TGCA'G Cuts at: 830 2139 2874 3758 830 Size: 1309 735 884 3478 Fragments arranged by size: 3478 1309 884 735 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6406 PvuII CAG'CTG Cuts at: 2363 2363 Size: 6406 SalI G'TCGA_C Cuts at: 1936 3748 1936 Size: 1812 4594 SapI GCTCTTCn'nnn_ Cuts at: 2564 2564 Size: 6406 SbfI CC_TGCA'GG Cuts at: 3758 3758 Size: 6406 ScaI AGT'ACT Cuts at: 4654 6366 4654 Size: 1712 4694 SfcI C'TryA_G Cuts at: 826 1045 2135 2870 3415 3754 4889 5570 5761 Size: 219 1090 735 545 339 1135 681 191 Cuts at: 5761 826 Size: 1471 Fragments arranged by size: 1471 1135 1090 735 681 545 339 219 191 SfoI GGC'GCC Cuts at: 2690 2730 2977 3316 2690 Size: 40 247 339 5780 Fragments arranged by size: 5780 339 247 40 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6406 SgrAI Cr'CCGG_yG Cuts at: 1700 2119 1700 Size: 419 5987 SmaI CCC'GGG Cuts at: 1872 2971 3018 3503 3733 1872 Size: 1099 47 485 230 4545 Fragments arranged by size: 4545 1099 485 230 47 SmlI C'TyrA_G Cuts at: 820 1017 1943 2920 3548 4510 5411 5673 820 Size: 197 926 977 628 962 901 262 1553 Fragments arranged by size: 1553 977 962 926 901 628 262 197 SnaBI TAC'GTA Cuts at: 493 4197 493 Size: 3704 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6406 SphI G_CATG'C Cuts at: 1132 3764 1132 Size: 2632 3774 SspI AAT'ATT Cuts at: 5 52 4330 5 Size: 47 4278 2081 Fragments arranged by size: 4278 2081 47 StuI AGG'CCT Cuts at: 1517 2072 1517 Size: 555 5851 StyI C'CwwG_G Cuts at: 513 1065 2886 3845 513 Size: 552 1821 959 3074 Fragments arranged by size: 3074 1821 959 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2915 3238 4433 4450 4603 4788 5233 Size: 1255 1335 323 1195 17 153 185 445 Cuts at: 5233 325 Size: 1498 Fragments arranged by size: 1498 1335 1255 1195 445 323 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3645 4652 6364 Size: 274 80 33 51 1443 1668 1007 1712 Cuts at: 6364 96 Size: 138 Fragments arranged by size: 1712 1668 1443 1007 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2466 2498 6366 6398 2466 Size: 32 3868 32 2474 Fragments arranged by size: 3868 2474 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6406 XbaI T'CTAG_A Cuts at: 3742 3742 Size: 6406 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6406 XmaI C'CCGG_G Cuts at: 1870 2969 3016 3501 3731 1870 Size: 1099 47 485 230 4545 Fragments arranged by size: 4545 1099 485 230 47 XmnI GAAnn'nnTTC Cuts at: 4535 4535 Size: 6406 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4214 276 Size: 53 83 186 936 2680 2468 Fragments arranged by size: 2680 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI