Restriction map for pFN21AE1054
 (Circular) (Six-base) MAPSORT of: pFN21AE1054.seq  Check: 8584  from: 1  to: 6316



pFN21AE1054  6316 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2657    2657 
   Size:       6316
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3270    4126     278 
   Size:         53      83     186     936    1734     856    2468

  Fragments arranged by size: 

                2468    1734     936     856     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3659    1937 
   Size:       1722    4594
 
Acc65I G'GTAC_C

Cuts at:   3637    3637 
   Size:       6316
 
AclI AA'CG_TT

Cuts at:   1563    4443    4816    1563 
   Size:       2880     373    3063

  Fragments arranged by size: 

                3063    2880     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2933    4379    5763    6031    1632 
   Size:       1301    1446    1384     268    1917

  Fragments arranged by size: 

                1917    1446    1384    1301     268
 
AfeI AGC'GCT

Cuts at:   2946    2946 
   Size:       6316
 
AflII C'TTAA_G

Cuts at:    820    1017    2752    3365     820 
   Size:        197    1735     613    3771

  Fragments arranged by size: 

                3771    1735     613     197
 
AflIII A'CryG_T

Cuts at:   1203    3007    5215    6192    1203 
   Size:       1804    2208     977    1327

  Fragments arranged by size: 

                2208    1804    1327     977
 
AgeI A'CCGG_T

Cuts at:   3281    5147    3281 
   Size:       1866    4450
 
AhdI GACnn_n'nnGTC

Cuts at:   5045    5045 
   Size:       6316
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       6316
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6316
 
AlwNI CAG_nnn'CTG

Cuts at:   3503    4059    5631    3503 
   Size:        556    1572    4188

  Fragments arranged by size: 

                4188    1572     556
 
ApaLI G'TGCA_C

Cuts at:   4373    5529    4373 
   Size:       1156    5160
 
ApoI r'AATT_y

Cuts at:   1493    2214    2315    3625    3851    4003    6112    1493 
   Size:        721     101    1310     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1310     721     226     152     101
 
AseI AT'TA_AT

Cuts at:    160    1032    4870     160 
   Size:        872    3838    1606

  Fragments arranged by size: 

                3838    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6316
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3641    1870 
   Size:         73    1698    4545

  Fragments arranged by size: 

                4545    1698      73
 
BamHI G'GATC_C

Cuts at:   3646    4013    3646 
   Size:        367    5949
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3637    5092     618 
   Size:        325     217     627    1850    1455    1842

  Fragments arranged by size: 

                1850    1842    1455     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2750    3635    1391 
   Size:       1359     885    4072

  Fragments arranged by size: 

                4072    1359     885
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    5345
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4507    4541    1903 
   Size:         34    2570      34    3678

  Fragments arranged by size: 

                3678    2570      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4208    5418    4208 
   Size:       1210    5106
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6316
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4927     136 
   Size:        107     122      71     631     605    3255    1525

  Fragments arranged by size: 

                3255    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2793    6311    2793 
   Size:       3518    2798
 
BlpI GC'TnA_GC

Cuts at:   3733    3733 
   Size:       6316
 
Bme1580I G_kGCm'C

Cuts at:   1790    4377    5533    1790 
   Size:       2587    1156    2573

  Fragments arranged by size: 

                2587    2573    1156
 
BmgBI CAC'GTC

Cuts at:   1318    2106    1318 
   Size:        788    5528
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3541    4047    5005 
   Size:        416      53      83     399      24    2124     506     958

Cuts at:   5005     442 
   Size:       1753

  Fragments arranged by size: 

                2124    1753     958     506     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6316
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3489    3489 
   Size:       6316
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2101    3026    4976    1239 
   Size:        862     925    1950    2579

  Fragments arranged by size: 

                2579    1950     925     862
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3560    4441    5306    5604    3560 
   Size:        881     865     298    4272

  Fragments arranged by size: 

                4272     881     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4979     882 
   Size:        617    3480    2219

  Fragments arranged by size: 

                3480    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4107     493 
   Size:        711    2903    2702

  Fragments arranged by size: 

                2903    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6310    6310 
   Size:       6316
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3267    4123    4505    5140 
   Size:         53      83     186     936    1734     856     382     635

Cuts at:   5140     275 
   Size:       1451

  Fragments arranged by size: 

                1734    1451     936     856     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2674    3281    4749    5147    5421    5568    5979    2674 
   Size:        607    1468     398     274     147     411    3011

  Fragments arranged by size: 

                3011    1468     607     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    6091

  Fragments arranged by size: 

                6091     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       6316
 
BseYI C'CCAG_C

Cuts at:   3403    5519    6015    3403 
   Size:       2116     496    3704

  Fragments arranged by size: 

                3704    2116     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2158    1163 
   Size:        573     422    5321

  Fragments arranged by size: 

                5321     573     422
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3286    3766    4527    5555    1540 
   Size:        275     186    1285     480     761    1028    2301

  Fragments arranged by size: 

                2301    1285    1028     761     480     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   4377    4462    5533    4377 
   Size:         85    1071    5160

  Fragments arranged by size: 

                5160    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2910    3827    3920    2910 
   Size:        917      93    5306

  Fragments arranged by size: 

                5306     917      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2750    3635    4377    4462    5533    1391 
   Size:        399     960     885     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     960     885     742     399      85
 
BspEI T'CCGG_A

Cuts at:   5979    5979 
   Size:       6316
 
BspHI T'CATG_A

Cuts at:   4205    5935    4205 
   Size:       1730    4586
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2361    2577    2657    3671     844 
   Size:       1517     216      80    1014    3489

  Fragments arranged by size: 

                3489    1517    1014     216      80
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4203    1394 
   Size:        429    2380    3507

  Fragments arranged by size: 

                3507    2380     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3427    4811    4985      65 
   Size:       1183    2179    1384     174    1396

  Fragments arranged by size: 

                2179    1396    1384    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3281    4960    5147    1700 
   Size:        110    1471    1679     187    2869

  Fragments arranged by size: 

                2869    1679    1471     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6316
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6316
 
BssSI C'ACGA_G

Cuts at:   4376    5388    4376 
   Size:       1012    5304
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6316
 
BstBI TT'CG_AA

Cuts at:   5120    5120 
   Size:       6316
 
BstEII G'GTnAC_C

Cuts at:   1184    2702    1184 
   Size:       1518    4798
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3554    3588    3554 
   Size:         34    6282
 
BstYI r'GATC_y

Cuts at:   1972    2130    2194    2793    3525    3646    4013    4398    4415 
   Size:        158      64     599     732     121     367     385      17

Cuts at:   4415    5867    5953    5965    6311    1972 
   Size:       1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1452     732     599     385     367     346     158
                 121      86      64      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2037    3031     513 
   Size:        552     972     994    3798

  Fragments arranged by size: 

                3798     994     972     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2206     487 
   Size:        683      56     980    4597

  Fragments arranged by size: 

                4597     980     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2125    3832    4626    4646    2125 
   Size:       1707     794      20    3795

  Fragments arranged by size: 

                3795    1707     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6316
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6281
 
DraI TTT'AAA

Cuts at:   3620    3620 
   Size:       6316
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    2392    5323     809 
   Size:        507    1076    2931    1802

  Fragments arranged by size: 

                2931    1802    1076     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2607    3763    4652 
   Size:         54    1425      50     275      18     777    1156     889

Cuts at:   4652    6281       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425    1156     889     777     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3763    1537 
   Size:        275    1951    4090

  Fragments arranged by size: 

                4090    1951     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    4246    1353 
   Size:       2893    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2822    4903    5277    5423     387 
   Size:       1064     138    1233    2081     374     146    1280

  Fragments arranged by size: 

                2081    1280    1233    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2101    2933    3026    4379    4976    5763    6031 
   Size:        393     469     832      93    1353     597     787     268

Cuts at:   6031    1239 
   Size:       1524

  Fragments arranged by size: 

                1524    1353     832     787     597     469     393     268
                  93
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2017    2017 
   Size:       6316
 
EcoRI G'AATT_C

Cuts at:   3625    4003    3625 
   Size:        378    5938
 
FspI TGC'GCA

Cuts at:   4822    4822 
   Size:       6316
 
HaeII r_GCGC'y

Cuts at:   1678    2948    5463    1678 
   Size:       1270    2515    2531

  Fragments arranged by size: 

                2531    2515    1270
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2470    2486    2502    2518    3115 
   Size:         32     135      32     898      16      16      16     597

Cuts at:   3115    3147    3416    3448    4957    4989    5031    5063    1373 
   Size:         32     269      32    1509      32      42      32    2626

  Fragments arranged by size: 

                2626    1509     898     597     269     135      42      32
                  32      32      32      32      32      16      16      16
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2842    3660    3906     669 
   Size:        988     281     904     818     246    3079

  Fragments arranged by size: 

                3079     988     904     818     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2268     748 
   Size:       1520    4796
 
HpaI GTT'AAC

Cuts at:   3906    3906 
   Size:       6316
 
KpnI G_GTAC'C

Cuts at:   3641    3641 
   Size:       6316
 
MfeI C'AATT_G

Cuts at:   2295    2581    3915    2295 
   Size:        286    1334    4696

  Fragments arranged by size: 

                4696    1334     286
 
MluI A'CGCG_T

Cuts at:   6192    6192 
   Size:       6316
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2912    5430    5614    1305 
   Size:       1607    2518     184    2007

  Fragments arranged by size: 

                2518    2007    1607     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    4494

  Fragments arranged by size: 

                4494    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2455    2724    3062    4274    4633    4792     518 
   Size:        648    1289     269     338    1212     359     159    2042

  Fragments arranged by size: 

                2042    1289    1212     648     359     338     269     159
            
 
MspA1I CmG'CkG

Cuts at:   1606    3046    3732    4409    5557    5802    1606 
   Size:       1440     686     677    1148     245    2120

  Fragments arranged by size: 

                2120    1440    1148     686     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2037     513 
   Size:        552     972    4792

  Fragments arranged by size: 

                4792     972     552
 
NdeI CA'TA_TG

Cuts at:    387    2721     387 
   Size:       2334    3982
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6316
 
NotI GC'GGCC_GC

Cuts at:   3763    3763 
   Size:       6316
 
NspI r_CATG'y

Cuts at:   1132    1229    2460    3011    3674    5219    1132 
   Size:         97    1231     551     663    1545    2229

  Fragments arranged by size: 

                2229    1545    1231     663     551      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6316
 
PciI A'CATG_T

Cuts at:   3007    5215    3007 
   Size:       2208    4108
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       6316
 
PfoI T'CCnGG_A

Cuts at:   2012    2012 
   Size:       6316
 
PmeI GTTT'AAAC

Cuts at:   3620    3620 
   Size:       6316
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4385    4417    5898    5930    4385 
   Size:         32    1481      32    4771

  Fragments arranged by size: 

                4771    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       6316
 
PsiI TTA'TAA

Cuts at:   3886    3886 
   Size:       6316
 
PstI C_TGCA'G

Cuts at:    830    2358    2574    3214    3668     830 
   Size:       1528     216     640     454    3478

  Fragments arranged by size: 

                3478    1528     640     454     216
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6316
 
PvuII CAG'CTG

Cuts at:   3046    3046 
   Size:       6316
 
SalI G'TCGA_C

Cuts at:   1936    3658    1936 
   Size:       1722    4594
 
SbfI CC_TGCA'GG

Cuts at:   3668    3668 
   Size:       6316
 
ScaI AGT'ACT

Cuts at:   3551    4564    6276    3551 
   Size:       1013    1712    3591

  Fragments arranged by size: 

                3591    1712    1013
 
SexAI A'CCwGG_T

Cuts at:   2706    2706 
   Size:       6316
 
SfcI C'TryA_G

Cuts at:    826    1045    2354    2570    2656    3210    3664    4799    5480 
   Size:        219    1309     216      86     554     454    1135     681

Cuts at:   5480    5671     826 
   Size:        191    1471

  Fragments arranged by size: 

                1471    1309    1135     681     554     454     219     216
                 191      86
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6316
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6316
 
SmaI CCC'GGG

Cuts at:   1872    3643    1872 
   Size:       1771    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2752    3365    3575    4420    5321    5583 
   Size:        197     926     809     613     210     845     901     262

Cuts at:   5583     820 
   Size:       1553

  Fragments arranged by size: 

                1553     926     901     845     809     613     262     210
                 197
 
SnaBI TAC'GTA

Cuts at:    493    4107     493 
   Size:       3614    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       6316
 
SphI G_CATG'C

Cuts at:   1132    3674    1132 
   Size:       2542    3774
 
SspI AAT'ATT

Cuts at:      5      52    2554    2973    4240       5 
   Size:         47    2502     419    1267    2081

  Fragments arranged by size: 

                2502    2081    1267     419      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6316
 
StyI C'CwwG_G

Cuts at:    513    1065    2037    3755     513 
   Size:        552     972    1718    3074

  Fragments arranged by size: 

                3074    1718     972     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3289    4343    4360    4513    4698    5143     325 
   Size:       1255    1709    1054      17     153     185     445    1498

  Fragments arranged by size: 

                1709    1498    1255    1054     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2526    2996    3549 
   Size:        274      80      33      51    1443     549     470     553

Cuts at:   3549    4562    6274      96 
   Size:       1013    1712     138

  Fragments arranged by size: 

                1712    1443    1013     553     549     470     274     138
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6276    6308    6276 
   Size:         32    6284
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6316
 
XbaI T'CTAG_A

Cuts at:   3652    3652 
   Size:       6316
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2949    2949 
   Size:       6316
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6316
 
XmaI C'CCGG_G

Cuts at:   1870    3641    1870 
   Size:       1771    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2405    4445    2405 
   Size:       2040    4276
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3268    4124     276 
   Size:         53      83     186     936    1734     856    2468

  Fragments arranged by size: 

                2468    1734     936     856     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PshAI     PsiI     PstI
     PvuI    PvuII     SalI     SbfI     ScaI    SexAI     SfcI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI    BfrBI
   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII  EcoICRI EcoO109I
    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NruI     NsiI     PacI     PmlI    PpuMI   PspOMI    PspXI     PsrI
    RsrII     SacI    SacII    SanDI     SapI     SfiI     SfoI     SrfI
     SwaI
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