(Circular) (Six-base) MAPSORT of: pFN21AE1054.seq Check: 8584 from: 1 to: 6316 pFN21AE1054 6316 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2657 2657 Size: 6316 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3270 4126 278 Size: 53 83 186 936 1734 856 2468 Fragments arranged by size: 2468 1734 936 856 186 83 53 AccI GT'mk_AC Cuts at: 1937 3659 1937 Size: 1722 4594 Acc65I G'GTAC_C Cuts at: 3637 3637 Size: 6316 AclI AA'CG_TT Cuts at: 1563 4443 4816 1563 Size: 2880 373 3063 Fragments arranged by size: 3063 2880 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2933 4379 5763 6031 1632 Size: 1301 1446 1384 268 1917 Fragments arranged by size: 1917 1446 1384 1301 268 AfeI AGC'GCT Cuts at: 2946 2946 Size: 6316 AflII C'TTAA_G Cuts at: 820 1017 2752 3365 820 Size: 197 1735 613 3771 Fragments arranged by size: 3771 1735 613 197 AflIII A'CryG_T Cuts at: 1203 3007 5215 6192 1203 Size: 1804 2208 977 1327 Fragments arranged by size: 2208 1804 1327 977 AgeI A'CCGG_T Cuts at: 3281 5147 3281 Size: 1866 4450 AhdI GACnn_n'nnGTC Cuts at: 5045 5045 Size: 6316 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6316 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6316 AlwNI CAG_nnn'CTG Cuts at: 3503 4059 5631 3503 Size: 556 1572 4188 Fragments arranged by size: 4188 1572 556 ApaLI G'TGCA_C Cuts at: 4373 5529 4373 Size: 1156 5160 ApoI r'AATT_y Cuts at: 1493 2214 2315 3625 3851 4003 6112 1493 Size: 721 101 1310 226 152 2109 1697 Fragments arranged by size: 2109 1697 1310 721 226 152 101 AseI AT'TA_AT Cuts at: 160 1032 4870 160 Size: 872 3838 1606 Fragments arranged by size: 3838 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6316 AvaI C'yCGr_G Cuts at: 1870 1943 3641 1870 Size: 73 1698 4545 Fragments arranged by size: 4545 1698 73 BamHI G'GATC_C Cuts at: 3646 4013 3646 Size: 367 5949 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3637 5092 618 Size: 325 217 627 1850 1455 1842 Fragments arranged by size: 1850 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2750 3635 1391 Size: 1359 885 4072 Fragments arranged by size: 4072 1359 885 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5345 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4507 4541 1903 Size: 34 2570 34 3678 Fragments arranged by size: 3678 2570 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4208 5418 4208 Size: 1210 5106 BclI T'GATC_A Cuts at: 1548 1548 Size: 6316 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4927 136 Size: 107 122 71 631 605 3255 1525 Fragments arranged by size: 3255 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2793 6311 2793 Size: 3518 2798 BlpI GC'TnA_GC Cuts at: 3733 3733 Size: 6316 Bme1580I G_kGCm'C Cuts at: 1790 4377 5533 1790 Size: 2587 1156 2573 Fragments arranged by size: 2587 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2106 1318 Size: 788 5528 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3541 4047 5005 Size: 416 53 83 399 24 2124 506 958 Cuts at: 5005 442 Size: 1753 Fragments arranged by size: 2124 1753 958 506 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6316 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3489 3489 Size: 6316 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2101 3026 4976 1239 Size: 862 925 1950 2579 Fragments arranged by size: 2579 1950 925 862 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3560 4441 5306 5604 3560 Size: 881 865 298 4272 Fragments arranged by size: 4272 881 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4979 882 Size: 617 3480 2219 Fragments arranged by size: 3480 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4107 493 Size: 711 2903 2702 Fragments arranged by size: 2903 2702 711 BsaBI GATnn'nnATC Cuts at: 6310 6310 Size: 6316 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3267 4123 4505 5140 Size: 53 83 186 936 1734 856 382 635 Cuts at: 5140 275 Size: 1451 Fragments arranged by size: 1734 1451 936 856 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2674 3281 4749 5147 5421 5568 5979 2674 Size: 607 1468 398 274 147 411 3011 Fragments arranged by size: 3011 1468 607 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6091 Fragments arranged by size: 6091 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6316 BseYI C'CCAG_C Cuts at: 3403 5519 6015 3403 Size: 2116 496 3704 Fragments arranged by size: 3704 2116 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2158 1163 Size: 573 422 5321 Fragments arranged by size: 5321 573 422 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3286 3766 4527 5555 1540 Size: 275 186 1285 480 761 1028 2301 Fragments arranged by size: 2301 1285 1028 761 480 275 186 BsiHKAI G_wGCw'C Cuts at: 4377 4462 5533 4377 Size: 85 1071 5160 Fragments arranged by size: 5160 1071 85 BsmI GAATG_Cn' Cuts at: 2910 3827 3920 2910 Size: 917 93 5306 Fragments arranged by size: 5306 917 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2750 3635 4377 4462 5533 1391 Size: 399 960 885 742 85 1071 2174 Fragments arranged by size: 2174 1071 960 885 742 399 85 BspEI T'CCGG_A Cuts at: 5979 5979 Size: 6316 BspHI T'CATG_A Cuts at: 4205 5935 4205 Size: 1730 4586 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2361 2577 2657 3671 844 Size: 1517 216 80 1014 3489 Fragments arranged by size: 3489 1517 1014 216 80 BsrBI CCG'CTC Cuts at: 1394 1823 4203 1394 Size: 429 2380 3507 Fragments arranged by size: 3507 2380 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3427 4811 4985 65 Size: 1183 2179 1384 174 1396 Fragments arranged by size: 2179 1396 1384 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3281 4960 5147 1700 Size: 110 1471 1679 187 2869 Fragments arranged by size: 2869 1679 1471 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6316 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6316 BssSI C'ACGA_G Cuts at: 4376 5388 4376 Size: 1012 5304 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6316 BstBI TT'CG_AA Cuts at: 5120 5120 Size: 6316 BstEII G'GTnAC_C Cuts at: 1184 2702 1184 Size: 1518 4798 BstXI CCAn_nnnn'nTGG Cuts at: 3554 3588 3554 Size: 34 6282 BstYI r'GATC_y Cuts at: 1972 2130 2194 2793 3525 3646 4013 4398 4415 Size: 158 64 599 732 121 367 385 17 Cuts at: 4415 5867 5953 5965 6311 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 732 599 385 367 346 158 121 86 64 17 12 BtgI C'CryG_G Cuts at: 513 1065 2037 3031 513 Size: 552 972 994 3798 Fragments arranged by size: 3798 994 972 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2206 487 Size: 683 56 980 4597 Fragments arranged by size: 4597 980 683 56 BtsI GCAGTG_nn' Cuts at: 2125 3832 4626 4646 2125 Size: 1707 794 20 3795 Fragments arranged by size: 3795 1707 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6316 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6281 DraI TTT'AAA Cuts at: 3620 3620 Size: 6316 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2392 5323 809 Size: 507 1076 2931 1802 Fragments arranged by size: 2931 1802 1076 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2607 3763 4652 Size: 54 1425 50 275 18 777 1156 889 Cuts at: 4652 6281 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1156 889 777 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3763 1537 Size: 275 1951 4090 Fragments arranged by size: 4090 1951 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4246 1353 Size: 2893 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2822 4903 5277 5423 387 Size: 1064 138 1233 2081 374 146 1280 Fragments arranged by size: 2081 1280 1233 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2101 2933 3026 4379 4976 5763 6031 Size: 393 469 832 93 1353 597 787 268 Cuts at: 6031 1239 Size: 1524 Fragments arranged by size: 1524 1353 832 787 597 469 393 268 93 EcoNI CCTnn'n_nnAGG Cuts at: 2017 2017 Size: 6316 EcoRI G'AATT_C Cuts at: 3625 4003 3625 Size: 378 5938 FspI TGC'GCA Cuts at: 4822 4822 Size: 6316 HaeII r_GCGC'y Cuts at: 1678 2948 5463 1678 Size: 1270 2515 2531 Fragments arranged by size: 2531 2515 1270 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2470 2486 2502 2518 3115 Size: 32 135 32 898 16 16 16 597 Cuts at: 3115 3147 3416 3448 4957 4989 5031 5063 1373 Size: 32 269 32 1509 32 42 32 2626 Fragments arranged by size: 2626 1509 898 597 269 135 42 32 32 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 669 1657 1938 2842 3660 3906 669 Size: 988 281 904 818 246 3079 Fragments arranged by size: 3079 988 904 818 281 246 HindIII A'AGCT_T Cuts at: 748 2268 748 Size: 1520 4796 HpaI GTT'AAC Cuts at: 3906 3906 Size: 6316 KpnI G_GTAC'C Cuts at: 3641 3641 Size: 6316 MfeI C'AATT_G Cuts at: 2295 2581 3915 2295 Size: 286 1334 4696 Fragments arranged by size: 4696 1334 286 MluI A'CGCG_T Cuts at: 6192 6192 Size: 6316 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2912 5430 5614 1305 Size: 1607 2518 184 2007 Fragments arranged by size: 2518 2007 1607 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4494 Fragments arranged by size: 4494 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2455 2724 3062 4274 4633 4792 518 Size: 648 1289 269 338 1212 359 159 2042 Fragments arranged by size: 2042 1289 1212 648 359 338 269 159 MspA1I CmG'CkG Cuts at: 1606 3046 3732 4409 5557 5802 1606 Size: 1440 686 677 1148 245 2120 Fragments arranged by size: 2120 1440 1148 686 677 245 NcoI C'CATG_G Cuts at: 513 1065 2037 513 Size: 552 972 4792 Fragments arranged by size: 4792 972 552 NdeI CA'TA_TG Cuts at: 387 2721 387 Size: 2334 3982 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6316 NotI GC'GGCC_GC Cuts at: 3763 3763 Size: 6316 NspI r_CATG'y Cuts at: 1132 1229 2460 3011 3674 5219 1132 Size: 97 1231 551 663 1545 2229 Fragments arranged by size: 2229 1545 1231 663 551 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6316 PciI A'CATG_T Cuts at: 3007 5215 3007 Size: 2208 4108 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6316 PfoI T'CCnGG_A Cuts at: 2012 2012 Size: 6316 PmeI GTTT'AAAC Cuts at: 3620 3620 Size: 6316 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4385 4417 5898 5930 4385 Size: 32 1481 32 4771 Fragments arranged by size: 4771 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6316 PsiI TTA'TAA Cuts at: 3886 3886 Size: 6316 PstI C_TGCA'G Cuts at: 830 2358 2574 3214 3668 830 Size: 1528 216 640 454 3478 Fragments arranged by size: 3478 1528 640 454 216 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6316 PvuII CAG'CTG Cuts at: 3046 3046 Size: 6316 SalI G'TCGA_C Cuts at: 1936 3658 1936 Size: 1722 4594 SbfI CC_TGCA'GG Cuts at: 3668 3668 Size: 6316 ScaI AGT'ACT Cuts at: 3551 4564 6276 3551 Size: 1013 1712 3591 Fragments arranged by size: 3591 1712 1013 SexAI A'CCwGG_T Cuts at: 2706 2706 Size: 6316 SfcI C'TryA_G Cuts at: 826 1045 2354 2570 2656 3210 3664 4799 5480 Size: 219 1309 216 86 554 454 1135 681 Cuts at: 5480 5671 826 Size: 191 1471 Fragments arranged by size: 1471 1309 1135 681 554 454 219 216 191 86 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6316 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6316 SmaI CCC'GGG Cuts at: 1872 3643 1872 Size: 1771 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2752 3365 3575 4420 5321 5583 Size: 197 926 809 613 210 845 901 262 Cuts at: 5583 820 Size: 1553 Fragments arranged by size: 1553 926 901 845 809 613 262 210 197 SnaBI TAC'GTA Cuts at: 493 4107 493 Size: 3614 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6316 SphI G_CATG'C Cuts at: 1132 3674 1132 Size: 2542 3774 SspI AAT'ATT Cuts at: 5 52 2554 2973 4240 5 Size: 47 2502 419 1267 2081 Fragments arranged by size: 2502 2081 1267 419 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6316 StyI C'CwwG_G Cuts at: 513 1065 2037 3755 513 Size: 552 972 1718 3074 Fragments arranged by size: 3074 1718 972 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3289 4343 4360 4513 4698 5143 325 Size: 1255 1709 1054 17 153 185 445 1498 Fragments arranged by size: 1709 1498 1255 1054 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2526 2996 3549 Size: 274 80 33 51 1443 549 470 553 Cuts at: 3549 4562 6274 96 Size: 1013 1712 138 Fragments arranged by size: 1712 1443 1013 553 549 470 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6276 6308 6276 Size: 32 6284 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6316 XbaI T'CTAG_A Cuts at: 3652 3652 Size: 6316 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2949 2949 Size: 6316 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6316 XmaI C'CCGG_G Cuts at: 1870 3641 1870 Size: 1771 4545 XmnI GAAnn'nnTTC Cuts at: 2405 4445 2405 Size: 2040 4276 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3268 4124 276 Size: 53 83 186 936 1734 856 2468 Fragments arranged by size: 2468 1734 936 856 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI