(Circular) (Six-base) MAPSORT of: pFN21AB7752.seq Check: 2873 from: 1 to: 6175 pFN21AB7752 6175 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3985 278 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3518 1937 Size: 1581 4594 Acc65I G'GTAC_C Cuts at: 3496 3496 Size: 6175 AclI AA'CG_TT Cuts at: 1563 4302 4675 1563 Size: 2739 373 3063 Fragments arranged by size: 3063 2739 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2511 2632 2777 3281 4238 5622 5890 1632 Size: 879 121 145 504 957 1384 268 1917 Fragments arranged by size: 1917 1384 957 879 504 268 145 121 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5978 AflIII A'CryG_T Cuts at: 1203 5074 6051 1203 Size: 3871 977 1327 Fragments arranged by size: 3871 1327 977 AgeI A'CCGG_T Cuts at: 5006 5006 Size: 6175 AhdI GACnn_n'nnGTC Cuts at: 4904 4904 Size: 6175 AleI CACnn'nnGTG Cuts at: 1166 2687 2948 1166 Size: 1521 261 4393 Fragments arranged by size: 4393 1521 261 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6175 AlwNI CAG_nnn'CTG Cuts at: 2292 3918 5490 2292 Size: 1626 1572 2977 Fragments arranged by size: 2977 1626 1572 ApaLI G'TGCA_C Cuts at: 4232 5388 4232 Size: 1156 5019 ApoI r'AATT_y Cuts at: 1493 2573 3357 3484 3710 3862 5971 1493 Size: 1080 784 127 226 152 2109 1697 Fragments arranged by size: 2109 1697 1080 784 226 152 127 AseI AT'TA_AT Cuts at: 160 1032 4729 160 Size: 872 3697 1606 Fragments arranged by size: 3697 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 AvaI C'yCGr_G Cuts at: 1870 1943 2992 3369 3412 3500 1870 Size: 73 1049 377 43 88 4545 Fragments arranged by size: 4545 1049 377 88 73 43 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2188 2221 2188 Size: 33 6142 BamHI G'GATC_C Cuts at: 3505 3872 3505 Size: 367 5808 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3496 4951 618 Size: 325 217 627 1709 1455 1842 Fragments arranged by size: 1842 1709 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2484 3311 3320 3419 3494 1391 Size: 1093 827 9 99 75 4072 Fragments arranged by size: 4072 1093 827 99 75 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2745 3393 928 Size: 971 846 648 3710 Fragments arranged by size: 3710 971 846 648 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4366 4400 1903 Size: 34 2429 34 3678 Fragments arranged by size: 3678 2429 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4067 5277 4067 Size: 1210 4965 BclI T'GATC_A Cuts at: 1548 1548 Size: 6175 BfrBI ATG'CAT Cuts at: 3220 3220 Size: 6175 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2522 3171 4786 Size: 107 122 71 631 605 850 649 1615 Cuts at: 4786 136 Size: 1525 Fragments arranged by size: 1615 1525 850 649 631 605 122 107 71 BglII A'GATC_T Cuts at: 6170 6170 Size: 6175 BlpI GC'TnA_GC Cuts at: 3592 3592 Size: 6175 Bme1580I G_kGCm'C Cuts at: 1790 4236 5392 1790 Size: 2446 1156 2573 Fragments arranged by size: 2573 2446 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6175 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2918 3191 3906 Size: 416 53 83 399 24 1501 273 715 Cuts at: 3906 4864 442 Size: 958 1753 Fragments arranged by size: 1753 1501 958 715 416 399 273 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6175 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3424 3424 Size: 6175 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3356 4835 1239 Size: 2117 1479 2579 Fragments arranged by size: 2579 2117 1479 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2849 3272 4300 5165 5463 2849 Size: 423 1028 865 298 3561 Fragments arranged by size: 3561 1028 865 423 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4838 882 Size: 617 3339 2219 Fragments arranged by size: 3339 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3966 493 Size: 711 2762 2702 Fragments arranged by size: 2762 2702 711 BsaBI GATnn'nnATC Cuts at: 6169 6169 Size: 6175 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2895 3186 3982 4364 Size: 53 83 186 936 1362 291 796 382 Cuts at: 4364 4999 275 Size: 635 1451 Fragments arranged by size: 1451 1362 936 796 635 382 291 186 83 53 BsaWI w'CCGG_w Cuts at: 3433 4608 5006 5280 5427 5838 3433 Size: 1175 398 274 147 411 3770 Fragments arranged by size: 3770 1175 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2021 2051 2616 2646 2765 Size: 30 165 30 618 30 565 30 119 Cuts at: 2765 2795 1178 Size: 30 4558 Fragments arranged by size: 4558 618 565 165 119 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 2462 3409 3440 1186 Size: 877 399 947 31 3921 Fragments arranged by size: 3921 947 877 399 31 BseYI C'CCAG_C Cuts at: 5378 5874 5378 Size: 496 5679 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3146 1163 Size: 573 1410 4192 Fragments arranged by size: 4192 1410 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3170 3625 4386 5414 1540 Size: 275 186 1169 455 761 1028 2301 Fragments arranged by size: 2301 1169 1028 761 455 275 186 BsiHKAI G_wGCw'C Cuts at: 3057 3419 4236 4321 5392 3057 Size: 362 817 85 1071 3840 Fragments arranged by size: 3840 1071 817 362 85 BsmI GAATG_Cn' Cuts at: 3222 3686 3779 3222 Size: 464 93 5618 Fragments arranged by size: 5618 464 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3335 3335 Size: 6175 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2484 3057 3311 3320 3419 3494 4236 Size: 399 694 573 254 9 99 75 742 Cuts at: 4236 4321 5392 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 694 573 399 254 99 85 75 9 BspEI T'CCGG_A Cuts at: 5838 5838 Size: 6175 BspHI T'CATG_A Cuts at: 4064 5794 4064 Size: 1730 4445 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3048 3229 3530 844 Size: 2204 181 301 3489 Fragments arranged by size: 3489 2204 301 181 BsrBI CCG'CTC Cuts at: 1394 1823 2434 2991 3374 4062 1394 Size: 429 611 557 383 688 3507 Fragments arranged by size: 3507 688 611 557 429 383 BsrDI GCAATG_nn' Cuts at: 65 1248 2511 3466 4670 4844 65 Size: 1183 1263 955 1204 174 1396 Fragments arranged by size: 1396 1263 1204 1183 955 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3165 3289 4819 5006 1700 Size: 110 1355 124 1530 187 2869 Fragments arranged by size: 2869 1530 1355 187 124 110 BsrGI T'GTAC_A Cuts at: 96 3141 96 Size: 3045 3130 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6175 BssSI C'ACGA_G Cuts at: 4235 5247 4235 Size: 1012 5163 BstAPI GCAn_nnn'nTGC Cuts at: 794 2322 2968 3461 794 Size: 1528 646 493 3508 Fragments arranged by size: 3508 1528 646 493 BstBI TT'CG_AA Cuts at: 4979 4979 Size: 6175 BstEII G'GTnAC_C Cuts at: 1184 2064 1184 Size: 880 5295 BstXI CCAn_nnnn'nTGG Cuts at: 2374 2374 Size: 6175 BstYI r'GATC_y Cuts at: 1972 2500 3505 3872 4257 4274 5726 5812 5824 Size: 528 1005 367 385 17 1452 86 12 Cuts at: 5824 6170 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1005 528 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2355 2367 513 Size: 552 939 351 12 4321 Fragments arranged by size: 4321 939 552 351 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2335 2513 487 Size: 683 56 1109 178 4149 Fragments arranged by size: 4149 1109 683 178 56 BtsI GCAGTG_nn' Cuts at: 2165 2462 3403 3691 4485 4505 2165 Size: 297 941 288 794 20 3835 Fragments arranged by size: 3835 941 794 297 288 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6175 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2007 2042 529 Size: 35 1443 35 4662 Fragments arranged by size: 4662 1443 35 35 DraI TTT'AAA Cuts at: 3479 3479 Size: 6175 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5182 809 Size: 507 3866 1802 Fragments arranged by size: 3866 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2095 2364 2400 Size: 54 1425 50 275 18 265 269 36 Cuts at: 2400 2593 2676 3167 3622 4511 6140 8 Size: 193 83 491 455 889 1629 43 Fragments arranged by size: 1629 1425 889 491 455 275 269 265 193 83 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 3167 3622 1537 Size: 275 1355 455 4090 Fragments arranged by size: 4090 1355 455 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2487 3017 3308 4105 1353 Size: 1134 530 291 797 3423 Fragments arranged by size: 3423 1134 797 530 291 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2455 2584 3419 4762 5136 5282 Size: 1064 138 866 129 835 1343 374 146 Cuts at: 5282 387 Size: 1280 Fragments arranged by size: 1343 1280 1064 866 835 374 146 138 129 EcoICRI GAG'CTC Cuts at: 3417 3417 Size: 6175 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2511 2632 2777 3281 3356 4238 4835 Size: 393 879 121 145 504 75 882 597 Cuts at: 4835 5622 5890 1239 Size: 787 268 1524 Fragments arranged by size: 1524 882 879 787 597 504 393 268 145 121 75 EcoNI CCTnn'n_nnAGG Cuts at: 2756 2756 Size: 6175 EcoO109I rG'GnC_Cy Cuts at: 3448 3448 Size: 6175 EcoRI G'AATT_C Cuts at: 3357 3484 3862 3357 Size: 127 378 5670 Fragments arranged by size: 5670 378 127 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2248 2248 Size: 6175 FseI GG_CCGG'CC Cuts at: 3169 3169 Size: 6175 FspI TGC'GCA Cuts at: 4681 4681 Size: 6175 HaeII r_GCGC'y Cuts at: 1678 5322 1678 Size: 3644 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 2765 2797 4816 Size: 32 135 32 449 32 712 32 2019 Cuts at: 4816 4848 4890 4922 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2019 712 449 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2982 3064 3519 3765 669 Size: 988 281 1044 82 455 246 3079 Fragments arranged by size: 3079 1044 988 455 281 246 82 HindIII A'AGCT_T Cuts at: 748 748 Size: 6175 HpaI GTT'AAC Cuts at: 3765 3765 Size: 6175 KpnI G_GTAC'C Cuts at: 3500 3500 Size: 6175 MfeI C'AATT_G Cuts at: 3774 3774 Size: 6175 MluI A'CGCG_T Cuts at: 6051 6051 Size: 6175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2916 5289 5473 1305 Size: 1611 2373 184 2007 Fragments arranged by size: 2373 2007 1611 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2366 2402 2595 10 Size: 54 1425 343 534 36 193 3590 Fragments arranged by size: 3590 1425 534 343 193 54 36 MslI CAynn'nnrTG Cuts at: 518 1166 2354 2366 2687 2948 4133 4492 4651 Size: 648 1188 12 321 261 1185 359 159 Cuts at: 4651 518 Size: 2042 Fragments arranged by size: 2042 1188 1185 648 359 321 261 159 12 MspA1I CmG'CkG Cuts at: 1606 2202 2289 2329 3155 3591 4268 5416 5661 Size: 596 87 40 826 436 677 1148 245 Cuts at: 5661 1606 Size: 2120 Fragments arranged by size: 2120 1148 826 677 596 436 245 87 40 NaeI GCC'GGC Cuts at: 3167 3167 Size: 6175 NcoI C'CATG_G Cuts at: 513 1065 2004 2367 513 Size: 552 939 363 4321 Fragments arranged by size: 4321 939 552 363 NdeI CA'TA_TG Cuts at: 387 387 Size: 6175 NgoMIV G'CCGG_C Cuts at: 3165 3165 Size: 6175 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6175 NotI GC'GGCC_GC Cuts at: 3622 3622 Size: 6175 NsiI A_TGCA'T Cuts at: 3222 3222 Size: 6175 NspI r_CATG'y Cuts at: 1132 1229 2122 3224 3533 5078 1132 Size: 97 893 1102 309 1545 2229 Fragments arranged by size: 2229 1545 1102 893 309 97 PasI CC'CwG_GG Cuts at: 1348 2545 1348 Size: 1197 4978 PciI A'CATG_T Cuts at: 5074 5074 Size: 6175 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6175 PfoI T'CCnGG_A Cuts at: 3333 3333 Size: 6175 PmeI GTTT'AAAC Cuts at: 3479 3479 Size: 6175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4244 4276 5757 5789 4244 Size: 32 1481 32 4630 Fragments arranged by size: 4630 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3448 3448 Size: 6175 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6175 PsiI TTA'TAA Cuts at: 3745 3745 Size: 6175 PstI C_TGCA'G Cuts at: 830 2469 2964 3527 830 Size: 1639 495 563 3478 Fragments arranged by size: 3478 1639 563 495 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6175 PvuII CAG'CTG Cuts at: 2289 2329 2289 Size: 40 6135 SacI G_AGCT'C Cuts at: 3419 3419 Size: 6175 SalI G'TCGA_C Cuts at: 1936 3517 1936 Size: 1581 4594 SapI GCTCTTCn'nnn_ Cuts at: 3308 3308 Size: 6175 SbfI CC_TGCA'GG Cuts at: 3527 3527 Size: 6175 ScaI AGT'ACT Cuts at: 4423 6135 4423 Size: 1712 4463 SfcI C'TryA_G Cuts at: 826 1045 2465 2960 3523 4658 5339 5530 826 Size: 219 1420 495 563 1135 681 191 1471 Fragments arranged by size: 1471 1420 1135 681 563 495 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6175 SmaI CCC'GGG Cuts at: 1872 3502 1872 Size: 1630 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2864 3251 4279 5180 5442 820 Size: 197 926 921 387 1028 901 262 1553 Fragments arranged by size: 1553 1028 926 921 901 387 262 197 SnaBI TAC'GTA Cuts at: 493 3966 493 Size: 3473 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6175 SphI G_CATG'C Cuts at: 1132 3533 1132 Size: 2401 3774 SspI AAT'ATT Cuts at: 5 52 4099 5 Size: 47 4047 2081 Fragments arranged by size: 4047 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6175 StyI C'CwwG_G Cuts at: 513 1065 2004 2367 3283 3614 513 Size: 552 939 363 916 331 3074 Fragments arranged by size: 3074 939 916 552 363 331 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2617 4202 4219 4372 4557 5002 325 Size: 1255 1037 1585 17 153 185 445 1498 Fragments arranged by size: 1585 1498 1255 1037 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3141 4421 6133 Size: 274 80 33 51 1443 1164 1280 1712 Cuts at: 6133 96 Size: 138 Fragments arranged by size: 1712 1443 1280 1164 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2616 2648 3171 3203 6135 6167 2616 Size: 32 523 32 2932 32 2624 Fragments arranged by size: 2932 2624 523 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6175 XbaI T'CTAG_A Cuts at: 2380 3511 2380 Size: 1131 5044 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2686 2905 2686 Size: 219 5956 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6175 XmaI C'CCGG_G Cuts at: 1870 3500 1870 Size: 1630 4545 XmnI GAAnn'nnTTC Cuts at: 4304 4304 Size: 6175 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3983 276 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoRV FspAI KasI NarI NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI