Restriction map for
pFN21AB7752
(Linear) (Six-base) MAPSORT of: KIBB7752.seq Check: 5550 from: 1 to: 1470
KIBB7752.orf
With 182 enzymes: SgfI *
October 14, 2008 16:51 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 627 772 1276 1470
Size: 506 121 145 504 194
Fragments arranged by size:
506 504 194 145 121
AleI CACnn'nnGTG
Cuts at: 0 682 943 1470
Size: 682 261 527
Fragments arranged by size:
682 527 261
AlwNI CAG_nnn'CTG
Cuts at: 0 287 1470
Size: 287 1183
ApoI r'AATT_y
Cuts at: 0 568 1352 1470
Size: 568 784 118
Fragments arranged by size:
784 568 118
AvaI C'yCGr_G
Cuts at: 0 987 1364 1407 1470
Size: 987 377 43 63
Fragments arranged by size:
987 377 63 43
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 183 216 1470
Size: 183 33 1254
Fragments arranged by size:
1254 183 33
BanII G_rGCy'C
Cuts at: 0 479 1306 1315 1414 1470
Size: 479 827 9 99 56
Fragments arranged by size:
827 479 99 56 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 740 1388 1470
Size: 740 648 82
Fragments arranged by size:
740 648 82
BfrBI ATG'CAT
Cuts at: 0 1215 1470
Size: 1215 255
BglI GCCn_nnn'nGGC
Cuts at: 0 517 1166 1470
Size: 517 649 304
Fragments arranged by size:
649 517 304
BmrI ACTGGGnnnn_n'
Cuts at: 0 913 1186 1470
Size: 913 273 284
Fragments arranged by size:
913 284 273
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1419 1470
Size: 1419 51
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1351 1470
Size: 1351 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 844 1267 1470
Size: 844 423 203
Fragments arranged by size:
844 423 203
BsaHI Gr'CG_yC
Cuts at: 0 890 1181 1470
Size: 890 291 289
Fragments arranged by size:
890 291 289
BsaWI w'CCGG_w
Cuts at: 0 1428 1470
Size: 1428 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 16 46 611 641 760 790 1470
Size: 16 30 565 30 119 30 680
Fragments arranged by size:
680 565 119 30 30 30 16
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 457 1404 1435 1470
Size: 58 399 947 31 35
Fragments arranged by size:
947 399 58 35 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1141 1470
Size: 1141 329
BsiEI CG_ry'CG
Cuts at: 0 1165 1470
Size: 1165 305
BsiHKAI G_wGCw'C
Cuts at: 0 1052 1414 1470
Size: 1052 362 56
Fragments arranged by size:
1052 362 56
BsmI GAATG_Cn'
Cuts at: 0 1217 1470
Size: 1217 253
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1330 1470
Size: 1330 140
Bsp1286I G_dGCh'C
Cuts at: 0 479 1052 1306 1315 1414 1470
Size: 479 573 254 9 99 56
Fragments arranged by size:
573 479 254 99 56 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1043 1224 1470
Size: 1043 181 246
Fragments arranged by size:
1043 246 181
BsrBI CCG'CTC
Cuts at: 0 429 986 1369 1470
Size: 429 557 383 101
Fragments arranged by size:
557 429 383 101
BsrDI GCAATG_nn'
Cuts at: 0 506 1461 1470
Size: 506 955 9
Fragments arranged by size:
955 506 9
BsrFI r'CCGG_y
Cuts at: 0 1160 1284 1470
Size: 1160 124 186
Fragments arranged by size:
1160 186 124
BsrGI T'GTAC_A
Cuts at: 0 1136 1470
Size: 1136 334
BstAPI GCAn_nnn'nTGC
Cuts at: 0 317 963 1456 1470
Size: 317 646 493 14
Fragments arranged by size:
646 493 317 14
BstEII G'GTnAC_C
Cuts at: 0 59 1470
Size: 59 1411
BstXI CCAn_nnnn'nTGG
Cuts at: 0 369 1470
Size: 369 1101
BstYI r'GATC_y
Cuts at: 0 495 1470
Size: 495 975
BtgI C'CryG_G
Cuts at: 0 350 362 1470
Size: 350 12 1108
Fragments arranged by size:
1108 350 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 330 508 1470
Size: 330 178 962
Fragments arranged by size:
962 330 178
BtsI GCAGTG_nn'
Cuts at: 0 160 457 1398 1470
Size: 160 297 941 72
Fragments arranged by size:
941 297 160 72
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2 37 1470
Size: 2 35 1433
Fragments arranged by size:
1433 35 2
EaeI y'GGCC_r
Cuts at: 0 90 359 395 588 671 1162 1470
Size: 90 269 36 193 83 491 308
Fragments arranged by size:
491 308 269 193 90 83 36
EagI C'GGCC_G
Cuts at: 0 1162 1470
Size: 1162 308
EarI CTCTTCn'nnn_
Cuts at: 0 482 1012 1303 1470
Size: 482 530 291 167
Fragments arranged by size:
530 482 291 167
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 450 579 1414 1470
Size: 450 129 835 56
Fragments arranged by size:
835 450 129 56
EcoICRI GAG'CTC
Cuts at: 0 1412 1470
Size: 1412 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 627 772 1276 1351 1470
Size: 506 121 145 504 75 119
Fragments arranged by size:
506 504 145 121 119 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 751 1470
Size: 751 719
EcoO109I rG'GnC_Cy
Cuts at: 0 1443 1470
Size: 1443 27
EcoRI G'AATT_C
Cuts at: 0 1352 1470
Size: 1352 118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 243 1470
Size: 243 1227
FseI GG_CCGG'CC
Cuts at: 0 1164 1470
Size: 1164 306
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 760 792 1470
Size: 16 32 712 32 678
Fragments arranged by size:
712 678 32 32 16
HincII GTy'rAC
Cuts at: 0 977 1059 1470
Size: 977 82 411
Fragments arranged by size:
977 411 82
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 911 1470
Size: 911 559
MscI TGG'CCA
Cuts at: 0 361 397 590 1470
Size: 361 36 193 880
Fragments arranged by size:
880 361 193 36
MslI CAynn'nnrTG
Cuts at: 0 349 361 682 943 1470
Size: 349 12 321 261 527
Fragments arranged by size:
527 349 321 261 12
MspA1I CmG'CkG
Cuts at: 0 197 284 324 1150 1470
Size: 197 87 40 826 320
Fragments arranged by size:
826 320 197 87 40
NaeI GCC'GGC
Cuts at: 0 1162 1470
Size: 1162 308
NcoI C'CATG_G
Cuts at: 0 362 1470
Size: 362 1108
NgoMIV G'CCGG_C
Cuts at: 0 1160 1470
Size: 1160 310
NsiI A_TGCA'T
Cuts at: 0 1217 1470
Size: 1217 253
NspI r_CATG'y
Cuts at: 0 117 1219 1470
Size: 117 1102 251
Fragments arranged by size:
1102 251 117
PasI CC'CwG_GG
Cuts at: 0 540 1470
Size: 540 930
PfoI T'CCnGG_A
Cuts at: 0 1328 1470
Size: 1328 142
PpuMI rG'GwC_Cy
Cuts at: 0 1443 1470
Size: 1443 27
PstI C_TGCA'G
Cuts at: 0 464 959 1470
Size: 464 495 511
Fragments arranged by size:
511 495 464
PvuII CAG'CTG
Cuts at: 0 284 324 1470
Size: 284 40 1146
Fragments arranged by size:
1146 284 40
SacI G_AGCT'C
Cuts at: 0 1414 1470
Size: 1414 56
SapI GCTCTTCn'nnn_
Cuts at: 0 1303 1470
Size: 1303 167
SfcI C'TryA_G
Cuts at: 0 460 955 1470
Size: 460 495 515
Fragments arranged by size:
515 495 460
SmlI C'TyrA_G
Cuts at: 0 859 1246 1470
Size: 859 387 224
Fragments arranged by size:
859 387 224
StyI C'CwwG_G
Cuts at: 0 362 1278 1470
Size: 362 916 192
Fragments arranged by size:
916 362 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 612 1470
Size: 612 858
TatI w'GTAC_w
Cuts at: 0 1136 1470
Size: 1136 334
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 611 643 1166 1198 1470
Size: 611 32 523 32 272
Fragments arranged by size:
611 523 272 32 32
XbaI T'CTAG_A
Cuts at: 0 375 1470
Size: 375 1095
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 681 900 1470
Size: 681 219 570
Fragments arranged by size:
681 570 219
Enzymes that do cut:
AcuI AleI AlwNI ApoI AvaI BaeI BanII BbsI
BfrBI BglI BmrI BplI BpmI BpuEI BsaHI BsaWI
BsaXI BseRI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
Hin4I HincII MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NsiI NspI PasI PfoI PpuMI PstI PvuII
SacI SapI SfcI SmlI StyI TaqII TatI TstI
XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BamHI BanI BbeI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmtI Bpu10I BsaI
BsaAI BsaBI BseYI BsiWI BspEI BspHI BssHII BssSI
BstBI BstZ17I Bsu36I ClaI DraI DraIII DrdI EcoRV
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI NarI NdeI NheI NotI NruI PacI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XhoI XmaI
XmnI ZraI