(Circular) (Six-base) MAPSORT of: pFN21AB7600.seq Check: 8390 from: 1 to: 6007 pFN21AB7600 6007 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2126 2622 2126 Size: 496 5511 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2673 3817 278 Size: 53 83 186 936 1137 1144 2468 Fragments arranged by size: 2468 1144 1137 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2571 3350 1937 Size: 634 779 4594 Fragments arranged by size: 4594 779 634 Acc65I G'GTAC_C Cuts at: 3328 3328 Size: 6007 AclI AA'CG_TT Cuts at: 1563 4134 4507 1563 Size: 2571 373 3063 Fragments arranged by size: 3063 2571 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4070 5454 5722 1632 Size: 2438 1384 268 1917 Fragments arranged by size: 2438 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5810 AflIII A'CryG_T Cuts at: 1203 4906 5883 1203 Size: 3703 977 1327 Fragments arranged by size: 3703 1327 977 AgeI A'CCGG_T Cuts at: 2059 4838 2059 Size: 2779 3228 AhdI GACnn_n'nnGTC Cuts at: 2128 2674 4736 2128 Size: 546 2062 3399 Fragments arranged by size: 3399 2062 546 AleI CACnn'nnGTG Cuts at: 1166 2042 3065 1166 Size: 876 1023 4108 Fragments arranged by size: 4108 1023 876 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6007 AlwNI CAG_nnn'CTG Cuts at: 2027 2872 3013 3148 3750 5322 2027 Size: 845 141 135 602 1572 2712 Fragments arranged by size: 2712 1572 845 602 141 135 ApaI G_GGCC'C Cuts at: 2401 2939 2401 Size: 538 5469 ApaLI G'TGCA_C Cuts at: 4064 5220 4064 Size: 1156 4851 ApoI r'AATT_y Cuts at: 1493 3316 3542 3694 5803 1493 Size: 1823 226 152 2109 1697 Fragments arranged by size: 2109 1823 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4561 160 Size: 872 3529 1606 Fragments arranged by size: 3529 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 AvaI C'yCGr_G Cuts at: 1870 1943 2939 2976 3332 1870 Size: 73 996 37 356 4545 Fragments arranged by size: 4545 996 356 73 37 BamHI G'GATC_C Cuts at: 3337 3704 3337 Size: 367 5640 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3328 4783 618 Size: 325 217 627 1541 1455 1842 Fragments arranged by size: 1842 1541 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2239 2265 2401 2540 2726 2939 3326 1391 Size: 848 26 136 139 186 213 387 4072 Fragments arranged by size: 4072 848 387 213 186 139 136 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5036 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4198 4232 1903 Size: 34 2261 34 3678 Fragments arranged by size: 3678 2261 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3899 5109 3899 Size: 1210 4797 BclI T'GATC_A Cuts at: 1548 2106 2863 1548 Size: 558 757 4692 Fragments arranged by size: 4692 757 558 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2018 2636 3200 Size: 107 122 71 631 605 346 618 564 Cuts at: 3200 4618 136 Size: 1418 1525 Fragments arranged by size: 1525 1418 631 618 605 564 346 122 107 71 BglII A'GATC_T Cuts at: 6002 6002 Size: 6007 BlpI GC'TnA_GC Cuts at: 2238 2909 3424 2238 Size: 671 515 4821 Fragments arranged by size: 4821 671 515 Bme1580I G_kGCm'C Cuts at: 1790 2401 2800 2939 4068 5224 1790 Size: 611 399 139 1129 1156 2573 Fragments arranged by size: 2573 1156 1129 611 399 139 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6007 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2067 3738 4696 Size: 416 53 83 399 24 650 1671 958 Cuts at: 4696 442 Size: 1753 Fragments arranged by size: 1753 1671 958 650 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6007 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2279 2915 3095 4667 1239 Size: 1040 636 180 1572 2579 Fragments arranged by size: 2579 1572 1040 636 180 Bpu10I CC'TnA_GC Cuts at: 2831 2831 Size: 6007 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4132 4997 5295 4132 Size: 865 298 4844 Fragments arranged by size: 4844 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4670 882 Size: 617 3171 2219 Fragments arranged by size: 3171 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3249 3798 493 Size: 711 2045 549 2702 Fragments arranged by size: 2702 2045 711 549 BsaBI GATnn'nnATC Cuts at: 6001 6001 Size: 6007 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2520 2670 3814 4196 Size: 53 83 186 936 987 150 1144 382 Cuts at: 4196 4831 275 Size: 635 1451 Fragments arranged by size: 1451 1144 987 936 635 382 186 150 83 53 BsaWI w'CCGG_w Cuts at: 2059 4440 4838 5112 5259 5670 2059 Size: 2381 398 274 147 411 2396 Fragments arranged by size: 2396 2381 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2363 2393 1178 Size: 30 165 30 960 30 4792 Fragments arranged by size: 4792 960 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2330 2609 3311 1186 Size: 1144 279 702 3882 Fragments arranged by size: 3882 1144 702 279 BseYI C'CCAG_C Cuts at: 2581 2767 3006 5210 5706 2581 Size: 186 239 2204 496 2882 Fragments arranged by size: 2882 2204 496 239 186 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2498 2702 2879 3101 1163 Size: 573 762 204 177 222 4069 Fragments arranged by size: 4069 762 573 222 204 177 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3457 4218 5246 1540 Size: 275 186 1456 761 1028 2301 Fragments arranged by size: 2301 1456 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2763 4068 4153 5224 2763 Size: 1305 85 1071 3546 Fragments arranged by size: 3546 1305 1071 85 BsiWI C'GTAC_G Cuts at: 2713 2713 Size: 6007 BsmI GAATG_Cn' Cuts at: 3045 3159 3518 3611 3045 Size: 114 359 93 5441 Fragments arranged by size: 5441 359 114 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2239 2265 2401 2540 2726 2763 2800 Size: 399 449 26 136 139 186 37 37 Cuts at: 2800 2939 3326 4068 4153 5224 1391 Size: 139 387 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 449 399 387 186 139 139 136 85 37 37 26 BspEI T'CCGG_A Cuts at: 5670 5670 Size: 6007 BspHI T'CATG_A Cuts at: 3896 5626 3896 Size: 1730 4277 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2126 2622 2956 3362 844 Size: 1282 496 334 406 3489 Fragments arranged by size: 3489 1282 496 406 334 BsrBI CCG'CTC Cuts at: 1394 1823 2484 3894 1394 Size: 429 661 1410 3507 Fragments arranged by size: 3507 1410 661 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3000 4502 4676 65 Size: 1183 1752 1502 174 1396 Fragments arranged by size: 1752 1502 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2059 2513 4651 4838 1700 Size: 110 249 454 2138 187 2869 Fragments arranged by size: 2869 2138 454 249 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6007 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6007 BssSI C'ACGA_G Cuts at: 3254 4067 5079 3254 Size: 813 1012 4182 Fragments arranged by size: 4182 1012 813 BstAPI GCAn_nnn'nTGC Cuts at: 794 2141 794 Size: 1347 4660 BstBI TT'CG_AA Cuts at: 4811 4811 Size: 6007 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6007 BstXI CCAn_nnnn'nTGG Cuts at: 2080 2656 2080 Size: 576 5431 BstYI r'GATC_y Cuts at: 1972 3337 3704 4089 4106 5558 5644 5656 6002 Size: 1365 367 385 17 1452 86 12 346 Cuts at: 6002 1972 Size: 1977 Fragments arranged by size: 1977 1452 1365 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2343 2467 513 Size: 552 1278 124 4053 Fragments arranged by size: 4053 1278 552 124 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2629 487 Size: 683 56 1403 3865 Fragments arranged by size: 3865 1403 683 56 BtsI GCAGTG_nn' Cuts at: 3523 4317 4337 3523 Size: 794 20 5193 Fragments arranged by size: 5193 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6007 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5972 DraI TTT'AAA Cuts at: 3311 3311 Size: 6007 DraIII CAC_nnn'GTG Cuts at: 3217 3217 Size: 6007 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5014 809 Size: 507 3698 1802 Fragments arranged by size: 3698 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2433 3454 Size: 54 1425 50 275 18 261 342 1021 Cuts at: 3454 4343 5972 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1021 889 342 275 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3454 1537 Size: 275 1642 4090 Fragments arranged by size: 4090 1642 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3937 1353 Size: 2584 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2375 2618 4594 4968 5114 387 Size: 1064 138 786 243 1976 374 146 1280 Fragments arranged by size: 1976 1280 1064 786 374 243 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2279 2915 3095 4070 4667 5454 5722 Size: 393 647 636 180 975 597 787 268 Cuts at: 5722 1239 Size: 1524 Fragments arranged by size: 1524 975 787 647 636 597 393 268 180 EcoO109I rG'GnC_Cy Cuts at: 2208 2353 2397 2827 2935 2936 3052 3106 3145 Size: 145 44 430 108 1 116 54 39 Cuts at: 3145 3202 3260 2208 Size: 57 58 4955 Fragments arranged by size: 4955 430 145 116 108 58 57 54 44 39 1 EcoRI G'AATT_C Cuts at: 3316 3694 3316 Size: 378 5629 FspI TGC'GCA Cuts at: 4513 4513 Size: 6007 HaeII r_GCGC'y Cuts at: 1678 2421 2984 3195 5154 1678 Size: 743 563 211 1959 2531 Fragments arranged by size: 2531 1959 743 563 211 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2114 2146 2363 2395 2396 Size: 32 135 32 542 32 217 32 1 Cuts at: 2396 2428 2660 2692 4648 4680 4722 4754 1373 Size: 32 232 32 1956 32 42 32 2626 Fragments arranged by size: 2626 1956 542 232 217 135 42 32 32 32 32 32 32 32 32 1 HincII GTy'rAC Cuts at: 669 1657 1938 2572 3351 3597 669 Size: 988 281 634 779 246 3079 Fragments arranged by size: 3079 988 779 634 281 246 HindIII A'AGCT_T Cuts at: 748 2774 748 Size: 2026 3981 HpaI GTT'AAC Cuts at: 3597 3597 Size: 6007 KpnI G_GTAC'C Cuts at: 3332 3332 Size: 6007 MfeI C'AATT_G Cuts at: 2441 3606 2441 Size: 1165 4842 MluI A'CGCG_T Cuts at: 5883 5883 Size: 6007 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2292 2427 2571 2712 5121 5305 1305 Size: 987 135 144 141 2409 184 2007 Fragments arranged by size: 2409 2007 987 184 144 141 135 MscI TGG'CCA Cuts at: 10 64 1489 1832 2093 2435 10 Size: 54 1425 343 261 342 3582 Fragments arranged by size: 3582 1425 343 342 261 54 MslI CAynn'nnrTG Cuts at: 518 1166 2042 2754 3065 3965 4324 4483 518 Size: 648 876 712 311 900 359 159 2042 Fragments arranged by size: 2042 900 876 712 648 359 311 159 MspA1I CmG'CkG Cuts at: 1606 2232 2469 2692 2818 2913 3423 4100 5248 Size: 626 237 223 126 95 510 677 1148 Cuts at: 5248 5493 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 626 510 245 237 223 126 95 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5455 NdeI CA'TA_TG Cuts at: 387 387 Size: 6007 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6007 NotI GC'GGCC_GC Cuts at: 3454 3454 Size: 6007 NspI r_CATG'y Cuts at: 1132 1229 2101 3365 4910 1132 Size: 97 872 1264 1545 2229 Fragments arranged by size: 2229 1545 1264 872 97 PasI CC'CwG_GG Cuts at: 1348 3034 1348 Size: 1686 4321 PciI A'CATG_T Cuts at: 4906 4906 Size: 6007 PflMI CCAn_nnn'nTGG Cuts at: 1108 2407 3217 1108 Size: 1299 810 3898 Fragments arranged by size: 3898 1299 810 PfoI T'CCnGG_A Cuts at: 2994 3141 2994 Size: 147 5860 PmeI GTTT'AAAC Cuts at: 3311 3311 Size: 6007 PmlI CAC'GTG Cuts at: 3249 3249 Size: 6007 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4076 4108 5589 5621 4076 Size: 32 1481 32 4462 Fragments arranged by size: 4462 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2208 2353 3106 3145 3260 2208 Size: 145 753 39 115 4955 Fragments arranged by size: 4955 753 145 115 39 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6007 PsiI TTA'TAA Cuts at: 3577 3577 Size: 6007 PspOMI G'GGCC_C Cuts at: 2397 2935 2397 Size: 538 5469 PstI C_TGCA'G Cuts at: 830 2337 2817 3018 3359 830 Size: 1507 480 201 341 3478 Fragments arranged by size: 3478 1507 480 341 201 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6007 PvuII CAG'CTG Cuts at: 2818 2913 2818 Size: 95 5912 SacII CC_GC'GG Cuts at: 2470 2470 Size: 6007 SalI G'TCGA_C Cuts at: 1936 2570 3349 1936 Size: 634 779 4594 Fragments arranged by size: 4594 779 634 SanDI GG'GwC_CC Cuts at: 3260 3260 Size: 6007 SbfI CC_TGCA'GG Cuts at: 3359 3359 Size: 6007 ScaI AGT'ACT Cuts at: 4255 5967 4255 Size: 1712 4295 SexAI A'CCwGG_T Cuts at: 3075 3075 Size: 6007 SfcI C'TryA_G Cuts at: 826 1045 2333 2813 3014 3355 4490 5171 5362 Size: 219 1288 480 201 341 1135 681 191 Cuts at: 5362 826 Size: 1471 Fragments arranged by size: 1471 1288 1135 681 480 341 219 201 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6007 SmaI CCC'GGG Cuts at: 1872 2978 3334 1872 Size: 1106 356 4545 Fragments arranged by size: 4545 1106 356 SmlI C'TyrA_G Cuts at: 820 1017 1943 4111 5012 5274 820 Size: 197 926 2168 901 262 1553 Fragments arranged by size: 2168 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3798 493 Size: 3305 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6007 SphI G_CATG'C Cuts at: 1132 2101 3365 1132 Size: 969 1264 3774 Fragments arranged by size: 3774 1264 969 SspI AAT'ATT Cuts at: 5 52 3931 5 Size: 47 3879 2081 Fragments arranged by size: 3879 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6007 StyI C'CwwG_G Cuts at: 513 1065 2151 2367 2421 3446 513 Size: 552 1086 216 54 1025 3074 Fragments arranged by size: 3074 1086 1025 552 216 54 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2054 2453 4034 4051 4204 4389 4834 Size: 1255 474 399 1581 17 153 185 445 Cuts at: 4834 325 Size: 1498 Fragments arranged by size: 1581 1498 1255 474 445 399 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4253 5965 96 Size: 274 80 33 51 1443 2276 1712 138 Fragments arranged by size: 2276 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5967 5999 5967 Size: 32 5975 Tth111I GACn'n_nGTC Cuts at: 1316 2500 1316 Size: 1184 4823 XbaI T'CTAG_A Cuts at: 3343 3343 Size: 6007 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2122 2428 3160 2122 Size: 306 732 4969 Fragments arranged by size: 4969 732 306 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6007 XmaI C'CCGG_G Cuts at: 1870 2976 3332 1870 Size: 1106 356 4545 Fragments arranged by size: 4545 1106 356 XmnI GAAnn'nnTTC Cuts at: 4136 4136 Size: 6007 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2671 3815 276 Size: 53 83 186 936 1137 1144 2468 Fragments arranged by size: 2468 1144 1137 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SapI SfiI SfoI SrfI SwaI