(Circular) (Six-base) MAPSORT of: pFN21AE0199.seq Check: 7980 from: 1 to: 8023 pFN21AE0199 8023 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2976 2976 Size: 8023 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5833 278 Size: 53 83 186 936 4297 2468 Fragments arranged by size: 4297 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4056 5366 1937 Size: 2119 1310 4594 Fragments arranged by size: 4594 2119 1310 Acc65I G'GTAC_C Cuts at: 3581 5344 3581 Size: 1763 6260 AclI AA'CG_TT Cuts at: 1563 6150 6523 1563 Size: 4587 373 3063 Fragments arranged by size: 4587 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2561 2978 3013 3450 3771 4230 4611 6086 Size: 929 417 35 437 321 459 381 1475 Cuts at: 6086 7470 7738 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1475 1384 929 459 437 417 381 321 268 35 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7826 AflIII A'CryG_T Cuts at: 1203 2997 3148 6922 7899 1203 Size: 1794 151 3774 977 1327 Fragments arranged by size: 3774 1794 1327 977 151 AgeI A'CCGG_T Cuts at: 3578 6854 3578 Size: 3276 4747 AhdI GACnn_n'nnGTC Cuts at: 3549 6752 3549 Size: 3203 4820 AleI CACnn'nnGTG Cuts at: 1166 2908 2982 4515 1166 Size: 1742 74 1533 4674 Fragments arranged by size: 4674 1742 1533 74 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8023 AlwNI CAG_nnn'CTG Cuts at: 4223 5179 5766 7338 4223 Size: 956 587 1572 4908 Fragments arranged by size: 4908 1572 956 587 ApaI G_GGCC'C Cuts at: 2086 2201 2439 3337 3608 4527 5244 2086 Size: 115 238 898 271 919 717 4865 Fragments arranged by size: 4865 919 898 717 271 238 115 ApaLI G'TGCA_C Cuts at: 2762 2911 6080 7236 2762 Size: 149 3169 1156 3549 Fragments arranged by size: 3549 3169 1156 149 ApoI r'AATT_y Cuts at: 1493 2738 4921 5303 5332 5558 5710 7819 1493 Size: 1245 2183 382 29 226 152 2109 1697 Fragments arranged by size: 2183 2109 1697 1245 382 226 152 29 AseI AT'TA_AT Cuts at: 160 1032 6577 160 Size: 872 5545 1606 Fragments arranged by size: 5545 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8023 AvaI C'yCGr_G Cuts at: 1870 1943 2484 2514 2666 3216 3546 5348 1870 Size: 73 541 30 152 550 330 1802 4545 Fragments arranged by size: 4545 1802 550 541 330 152 73 30 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3565 3573 3598 3606 3565 Size: 8 25 8 7982 Fragments arranged by size: 7982 25 8 8 BamHI G'GATC_C Cuts at: 2629 5353 5720 2629 Size: 2724 367 4932 Fragments arranged by size: 4932 2724 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2384 2925 3192 3220 3305 Size: 325 217 627 597 541 267 28 85 Cuts at: 3305 3581 4461 4492 5344 6799 618 Size: 276 880 31 852 1455 1842 Fragments arranged by size: 1842 1455 880 852 627 597 541 325 276 267 217 85 31 28 BanII G_rGCy'C Cuts at: 1391 2052 2086 2201 2396 2439 3246 3337 3608 Size: 661 34 115 195 43 807 91 271 Cuts at: 3608 4527 5172 5244 5342 1391 Size: 919 645 72 98 4072 Fragments arranged by size: 4072 919 807 661 645 271 195 115 98 91 72 43 34 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2966 3438 3839 928 Size: 971 1067 472 401 5112 Fragments arranged by size: 5112 1067 971 472 401 BbvCI CC'TCA_GC Cuts at: 2840 2840 Size: 8023 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3044 3078 6214 6248 1903 Size: 34 1107 34 3136 34 3678 Fragments arranged by size: 3678 3136 1107 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3881 3891 5915 7125 3881 Size: 10 2024 1210 4779 Fragments arranged by size: 4779 2024 1210 10 BclI T'GATC_A Cuts at: 1548 5200 1548 Size: 3652 4371 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3603 4264 5176 Size: 107 122 71 631 605 1931 661 912 Cuts at: 5176 6634 136 Size: 1458 1525 Fragments arranged by size: 1931 1525 1458 912 661 631 605 122 107 71 BglII A'GATC_T Cuts at: 8018 8018 Size: 8023 BlpI GC'TnA_GC Cuts at: 4224 4689 5440 4224 Size: 465 751 6807 Fragments arranged by size: 6807 751 465 Bme1580I G_kGCm'C Cuts at: 1790 2086 2201 2251 2439 2766 2915 3223 3337 Size: 296 115 50 188 327 149 308 114 Cuts at: 3337 3608 4466 4527 5244 6084 7240 1790 Size: 271 858 61 717 840 1156 2573 Fragments arranged by size: 2573 1156 858 840 717 327 308 296 271 188 149 115 114 61 50 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8023 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2067 2082 2827 Size: 416 53 83 399 24 650 15 745 Cuts at: 2827 2851 3331 3605 4460 4509 5754 6712 442 Size: 24 480 274 855 49 1245 958 1753 Fragments arranged by size: 1753 1245 958 855 745 650 480 416 399 274 83 53 49 24 24 15 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8023 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2415 5205 2415 Size: 2790 5233 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2337 2815 3424 3625 3939 4568 4818 6683 Size: 1098 478 609 201 314 629 250 1865 Cuts at: 6683 1239 Size: 2579 Fragments arranged by size: 2579 1865 1098 629 609 478 314 250 201 Bpu10I CC'TnA_GC Cuts at: 2840 2840 Size: 8023 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3518 4667 6148 7013 7311 3518 Size: 1149 1481 865 298 4230 Fragments arranged by size: 4230 1481 1149 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2171 3557 6686 882 Size: 617 672 1386 3129 2219 Fragments arranged by size: 3129 2219 1386 672 617 BsaAI yAC'GTr Cuts at: 493 1204 5814 493 Size: 711 4610 2702 Fragments arranged by size: 4610 2702 711 BsaBI GATnn'nnATC Cuts at: 2347 8017 2347 Size: 5670 2353 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3174 5830 6212 6847 Size: 53 83 186 936 1641 2656 382 635 Cuts at: 6847 275 Size: 1451 Fragments arranged by size: 2656 1641 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3578 6456 6854 7128 7275 7686 3578 Size: 2878 398 274 147 411 3915 Fragments arranged by size: 3915 2878 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2795 2825 3513 3543 3907 Size: 30 165 30 1392 30 688 30 364 Cuts at: 3907 3937 4715 4745 5215 5245 1178 Size: 30 778 30 470 30 3956 Fragments arranged by size: 3956 1392 778 688 470 364 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2690 3670 4560 5076 5178 1186 Size: 1504 980 890 516 102 4031 Fragments arranged by size: 4031 1504 980 890 516 102 BseYI C'CCAG_C Cuts at: 2312 2410 3563 3756 3928 4073 4305 4560 4602 Size: 98 1153 193 172 145 232 255 42 Cuts at: 4602 5030 7226 7722 2312 Size: 428 2196 496 2613 Fragments arranged by size: 2613 2196 1153 496 428 255 232 193 172 145 98 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2173 2244 3094 3396 1163 Size: 573 437 71 850 302 5790 Fragments arranged by size: 5790 850 573 437 302 71 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5473 6234 7262 1540 Size: 275 186 3472 761 1028 2301 Fragments arranged by size: 3472 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2766 2784 2915 3009 6084 6169 7240 2766 Size: 18 131 94 3075 85 1071 3549 Fragments arranged by size: 3549 3075 1071 131 94 85 18 BsmI GAATG_Cn' Cuts at: 4093 5534 5627 4093 Size: 1441 93 6489 Fragments arranged by size: 6489 1441 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2086 2201 2251 2396 2439 2766 Size: 399 262 34 115 50 145 43 327 Cuts at: 2766 2784 2915 3009 3223 3246 3337 3608 4466 Size: 18 131 94 214 23 91 271 858 Cuts at: 4466 4527 5172 5244 5342 6084 6169 7240 1391 Size: 61 645 72 98 742 85 1071 2174 Fragments arranged by size: 2174 1071 858 742 645 399 327 271 262 214 145 131 115 98 94 91 85 72 61 50 43 34 23 18 BspEI T'CCGG_A Cuts at: 7686 7686 Size: 8023 BspHI T'CATG_A Cuts at: 5912 7642 5912 Size: 1730 6293 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2295 2976 3774 5378 844 Size: 1451 681 798 1604 3489 Fragments arranged by size: 3489 1604 1451 798 681 BsrBI CCG'CTC Cuts at: 1394 1823 2797 3256 3678 5910 1394 Size: 429 974 459 422 2232 3507 Fragments arranged by size: 3507 2232 974 459 429 422 BsrDI GCAATG_nn' Cuts at: 65 1248 3040 3418 3462 4985 6518 6692 65 Size: 1183 1792 378 44 1523 1533 174 1396 Fragments arranged by size: 1792 1533 1523 1396 1183 378 174 44 BsrFI r'CCGG_y Cuts at: 1700 1810 3578 6667 6854 1700 Size: 110 1768 3089 187 2869 Fragments arranged by size: 3089 2869 1768 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8023 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8023 BssSI C'ACGA_G Cuts at: 3008 6083 7095 3008 Size: 3075 1012 3936 Fragments arranged by size: 3936 3075 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2761 3374 3766 3782 794 Size: 1967 613 392 16 5035 Fragments arranged by size: 5035 1967 613 392 16 BstBI TT'CG_AA Cuts at: 6827 6827 Size: 8023 BstEII G'GTnAC_C Cuts at: 1184 3907 1184 Size: 2723 5300 BstXI CCAn_nnnn'nTGG Cuts at: 2162 2465 4489 4795 2162 Size: 303 2024 306 5390 Fragments arranged by size: 5390 2024 306 303 BstYI r'GATC_y Cuts at: 1972 2629 3127 5282 5353 5720 6105 6122 7574 Size: 657 498 2155 71 367 385 17 1452 Cuts at: 7574 7660 7672 8018 1972 Size: 86 12 346 1977 Fragments arranged by size: 2155 1977 1452 657 498 385 367 346 86 71 17 12 Bsu36I CC'TnA_GG Cuts at: 3506 5046 3506 Size: 1540 6483 BtgI C'CryG_G Cuts at: 513 1065 2788 3196 4095 4140 513 Size: 552 1723 408 899 45 4396 Fragments arranged by size: 4396 1723 899 552 408 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2356 487 Size: 683 56 1130 6154 Fragments arranged by size: 6154 1130 683 56 BtsI GCAGTG_nn' Cuts at: 3358 3813 4156 5539 6333 6353 3358 Size: 455 343 1383 794 20 5028 Fragments arranged by size: 5028 1383 794 455 343 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8023 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7988 DraI TTT'AAA Cuts at: 5327 5327 Size: 8023 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7030 809 Size: 507 5714 1802 Fragments arranged by size: 5714 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3533 5470 6359 Size: 54 1425 50 275 18 1703 1937 889 Cuts at: 6359 7988 8 Size: 1629 43 Fragments arranged by size: 1937 1703 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5470 1537 Size: 275 3658 4090 Fragments arranged by size: 4090 3658 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3492 4113 5953 1353 Size: 2139 621 1840 3423 Fragments arranged by size: 3423 2139 1840 621 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6610 6984 7130 387 Size: 1064 138 5021 374 146 1280 Fragments arranged by size: 5021 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2337 2561 2815 2978 3013 3424 3450 Size: 393 705 224 254 163 35 411 26 Cuts at: 3450 3625 3771 3939 4230 4568 4611 4818 6086 Size: 175 146 168 291 338 43 207 1268 Cuts at: 6086 6683 7470 7738 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1268 787 705 597 411 393 338 291 268 254 224 207 175 168 163 146 43 35 26 EcoNI CCTnn'n_nnAGG Cuts at: 5091 5091 Size: 8023 EcoO109I rG'GnC_Cy Cuts at: 2082 2083 2197 2435 2436 2519 2662 2844 3266 Size: 1 114 238 1 83 143 182 422 Cuts at: 3266 3333 3455 3543 3605 4134 4205 4379 4523 Size: 67 122 88 62 529 71 174 144 Cuts at: 4523 4524 4592 5069 5241 2082 Size: 1 68 477 172 4864 Fragments arranged by size: 4864 529 477 422 238 182 174 172 144 143 122 114 88 83 71 68 67 62 1 1 1 EcoRI G'AATT_C Cuts at: 5303 5332 5710 5303 Size: 29 378 7616 Fragments arranged by size: 7616 378 29 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3801 3801 Size: 8023 FspI TGC'GCA Cuts at: 2882 6529 2882 Size: 3647 4376 HaeII r_GCGC'y Cuts at: 1678 7170 1678 Size: 5492 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2488 2520 2678 2710 3119 Size: 32 135 32 916 32 158 32 409 Cuts at: 3119 3151 3255 3287 3535 3567 3829 3861 6664 Size: 32 104 32 248 32 262 32 2803 Cuts at: 6664 6696 6738 6770 1373 Size: 32 42 32 2626 Fragments arranged by size: 2803 2626 916 409 262 248 158 135 104 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2235 5367 5613 669 Size: 988 281 297 3132 246 3079 Fragments arranged by size: 3132 3079 988 297 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8023 HpaI GTT'AAC Cuts at: 5613 5613 Size: 8023 KpnI G_GTAC'C Cuts at: 3585 5348 3585 Size: 1763 6260 MfeI C'AATT_G Cuts at: 5622 5622 Size: 8023 MluI A'CGCG_T Cuts at: 7899 7899 Size: 8023 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2250 7137 7321 1305 Size: 945 4887 184 2007 Fragments arranged by size: 4887 2007 945 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6201 Fragments arranged by size: 6201 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2160 2867 2908 2982 2998 3075 4515 Size: 648 994 707 41 74 16 77 1440 Cuts at: 4515 5255 5981 6340 6499 518 Size: 740 726 359 159 2042 Fragments arranged by size: 2042 1440 994 740 726 707 648 359 159 77 74 41 16 MspA1I CmG'CkG Cuts at: 1606 2187 2539 2758 4223 5034 5143 5206 5439 Size: 581 352 219 1465 811 109 63 233 Cuts at: 5439 6116 7264 7509 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1465 1148 811 677 581 352 245 233 219 109 63 NcoI C'CATG_G Cuts at: 513 1065 4095 4140 513 Size: 552 3030 45 4396 Fragments arranged by size: 4396 3030 552 45 NdeI CA'TA_TG Cuts at: 387 4846 387 Size: 4459 3564 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8023 NotI GC'GGCC_GC Cuts at: 5470 5470 Size: 8023 NruI TCG'CGA Cuts at: 2341 2341 Size: 8023 NspI r_CATG'y Cuts at: 1132 1229 2560 2866 2881 3001 3152 5381 6926 Size: 97 1331 306 15 120 151 2229 1545 Cuts at: 6926 1132 Size: 2229 Fragments arranged by size: 2229 2229 1545 1331 306 151 120 97 15 PasI CC'CwG_GG Cuts at: 1348 3329 5173 1348 Size: 1981 1844 4198 Fragments arranged by size: 4198 1981 1844 PciI A'CATG_T Cuts at: 2997 3148 6922 2997 Size: 151 3774 4098 Fragments arranged by size: 4098 3774 151 PflMI CCAn_nnn'nTGG Cuts at: 1108 3329 3722 4130 1108 Size: 2221 393 408 5001 Fragments arranged by size: 5001 2221 408 393 PfoI T'CCnGG_A Cuts at: 2353 3883 2353 Size: 1530 6493 PmeI GTTT'AAAC Cuts at: 5327 5327 Size: 8023 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6092 6124 7605 7637 6092 Size: 32 1481 32 6478 Fragments arranged by size: 6478 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2519 2662 3266 3455 3543 4134 4205 5069 2519 Size: 143 604 189 88 591 71 864 5473 Fragments arranged by size: 5473 864 604 591 189 143 88 71 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8023 PsiI TTA'TAA Cuts at: 5593 5593 Size: 8023 PspOMI G'GGCC_C Cuts at: 2082 2197 2435 3333 3604 4523 5240 2082 Size: 115 238 898 271 919 717 4865 Fragments arranged by size: 4865 919 898 717 271 238 115 PstI C_TGCA'G Cuts at: 830 2292 2732 3020 3350 3820 5375 830 Size: 1462 440 288 330 470 1555 3478 Fragments arranged by size: 3478 1555 1462 470 440 330 288 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8023 PvuII CAG'CTG Cuts at: 2187 2539 2758 4223 5034 5143 2187 Size: 352 219 1465 811 109 5067 Fragments arranged by size: 5067 1465 811 352 219 109 SalI G'TCGA_C Cuts at: 1936 5365 1936 Size: 3429 4594 SanDI GG'GwC_CC Cuts at: 2519 3543 2519 Size: 1024 6999 SapI GCTCTTCn'nnn_ Cuts at: 4113 4113 Size: 8023 SbfI CC_TGCA'GG Cuts at: 5375 5375 Size: 8023 ScaI AGT'ACT Cuts at: 6271 7983 6271 Size: 1712 6311 SfcI C'TryA_G Cuts at: 826 1045 2288 2728 3016 3234 3346 3816 4112 Size: 219 1243 440 288 218 112 470 296 Cuts at: 4112 4402 5371 6506 7187 7378 826 Size: 290 969 1135 681 191 1471 Fragments arranged by size: 1471 1243 1135 969 681 470 440 296 290 288 219 218 191 112 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8023 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8023 SmaI CCC'GGG Cuts at: 1872 2486 2516 3548 5350 1872 Size: 614 30 1032 1802 4545 Fragments arranged by size: 4545 1802 1032 614 30 SmlI C'TyrA_G Cuts at: 820 1017 1943 2666 3497 4682 6127 7028 7290 Size: 197 926 723 831 1185 1445 901 262 Cuts at: 7290 820 Size: 1553 Fragments arranged by size: 1553 1445 1185 926 901 831 723 262 197 SnaBI TAC'GTA Cuts at: 493 5814 493 Size: 5321 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8023 SphI G_CATG'C Cuts at: 1132 5381 1132 Size: 4249 3774 SspI AAT'ATT Cuts at: 5 52 5947 5 Size: 47 5895 2081 Fragments arranged by size: 5895 2081 47 StuI AGG'CCT Cuts at: 1517 4313 4639 1517 Size: 2796 326 4901 Fragments arranged by size: 4901 2796 326 StyI C'CwwG_G Cuts at: 513 1065 2586 3860 4095 4140 4383 5462 513 Size: 552 1521 1274 235 45 243 1079 3074 Fragments arranged by size: 3074 1521 1274 1079 552 243 235 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2326 3060 4018 4291 4346 4679 6050 Size: 1255 746 734 958 273 55 333 1371 Cuts at: 6050 6067 6220 6405 6850 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1371 1255 958 746 734 445 333 273 185 153 55 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2859 6269 7981 Size: 274 80 33 51 1443 882 3410 1712 Cuts at: 7981 96 Size: 138 Fragments arranged by size: 3410 1712 1443 882 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2795 2827 3907 3939 4715 4747 7983 8015 2795 Size: 32 1080 32 776 32 3236 32 2803 Fragments arranged by size: 3236 2803 1080 776 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8023 XbaI T'CTAG_A Cuts at: 5359 5359 Size: 8023 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3235 4642 5254 3235 Size: 1407 612 6004 Fragments arranged by size: 6004 1407 612 XhoI C'TCGA_G Cuts at: 1943 2666 1943 Size: 723 7300 XmaI C'CCGG_G Cuts at: 1870 2484 2514 3546 5348 1870 Size: 614 30 1032 1802 4545 Fragments arranged by size: 4545 1802 1032 614 30 XmnI GAAnn'nnTTC Cuts at: 2804 6152 2804 Size: 3348 4675 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5831 276 Size: 53 83 186 936 4297 2468 Fragments arranged by size: 4297 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI