(Circular) (Six-base) MAPSORT of: pFN21AA0133.seq Check: 8283 from: 1 to: 9277 pFN21AA0133 9277 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2410 2613 2674 3030 2410 Size: 203 61 356 8657 Fragments arranged by size: 8657 356 203 61 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6462 7087 278 Size: 53 83 186 936 4926 625 2468 Fragments arranged by size: 4926 2468 936 625 186 83 53 AccI GT'mk_AC Cuts at: 1937 2396 6620 1937 Size: 459 4224 4594 Fragments arranged by size: 4594 4224 459 Acc65I G'GTAC_C Cuts at: 6598 6598 Size: 9277 AclI AA'CG_TT Cuts at: 1563 7404 7777 1563 Size: 5841 373 3063 Fragments arranged by size: 5841 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2210 2273 3260 3285 4104 6028 6098 7340 Size: 578 63 987 25 819 1924 70 1242 Cuts at: 7340 8724 8992 1632 Size: 1384 268 1917 Fragments arranged by size: 1924 1917 1384 1242 987 819 578 268 70 63 25 AflII C'TTAA_G Cuts at: 820 1017 2033 820 Size: 197 1016 8064 Fragments arranged by size: 8064 1016 197 AflIII A'CryG_T Cuts at: 1203 3540 5778 6363 8176 9153 1203 Size: 2337 2238 585 1813 977 1327 Fragments arranged by size: 2337 2238 1813 1327 977 585 AgeI A'CCGG_T Cuts at: 8108 8108 Size: 9277 AhdI GACnn_n'nnGTC Cuts at: 3544 5768 8006 3544 Size: 2224 2238 4815 Fragments arranged by size: 4815 2238 2224 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9277 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2267 3615 3704 1184 Size: 1083 1348 89 6757 Fragments arranged by size: 6757 1348 1083 89 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3845 3877 4613 4645 3845 Size: 32 736 32 8477 Fragments arranged by size: 8477 736 32 32 AlwNI CAG_nnn'CTG Cuts at: 2437 2689 3449 3535 3751 5230 5294 5488 5642 Size: 252 760 86 216 1479 64 194 154 Cuts at: 5642 5764 6469 7020 8592 2437 Size: 122 705 551 1572 3122 Fragments arranged by size: 3122 1572 1479 760 705 551 252 216 194 154 122 86 64 ApaI G_GGCC'C Cuts at: 3485 3847 3903 5326 3485 Size: 362 56 1423 7436 Fragments arranged by size: 7436 1423 362 56 ApaLI G'TGCA_C Cuts at: 6276 7334 8490 6276 Size: 1058 1156 7063 Fragments arranged by size: 7063 1156 1058 ApoI r'AATT_y Cuts at: 1493 3141 4991 5646 6586 6812 6964 9073 1493 Size: 1648 1850 655 940 226 152 2109 1697 Fragments arranged by size: 2109 1850 1697 1648 940 655 226 152 AseI AT'TA_AT Cuts at: 160 1032 2283 7831 160 Size: 872 1251 5548 1606 Fragments arranged by size: 5548 1606 1251 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9277 AvaI C'yCGr_G Cuts at: 1870 1943 3478 5325 6106 6602 1870 Size: 73 1535 1847 781 496 4545 Fragments arranged by size: 4545 1847 1535 781 496 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4019 4052 4019 Size: 33 9244 BamHI G'GATC_C Cuts at: 2345 6607 6974 2345 Size: 4262 367 4648 Fragments arranged by size: 4648 4262 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4481 5334 6598 8053 618 Size: 325 217 627 2694 853 1264 1455 1842 Fragments arranged by size: 2694 1842 1455 1264 853 627 325 217 BanII G_rGCy'C Cuts at: 1391 2778 3485 3741 3847 3903 5326 6516 6596 Size: 1387 707 256 106 56 1423 1190 80 Cuts at: 6596 1391 Size: 4072 Fragments arranged by size: 4072 1423 1387 1190 707 256 106 80 56 BbeI G_GCGC'C Cuts at: 5338 5338 Size: 9277 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2810 3248 3364 6094 928 Size: 971 911 438 116 2730 4111 Fragments arranged by size: 4111 2730 971 911 438 116 BbvCI CC'TCA_GC Cuts at: 2678 3450 5825 6321 2678 Size: 772 2375 496 5634 Fragments arranged by size: 5634 2375 772 496 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7468 7502 1903 Size: 34 5531 34 3678 Fragments arranged by size: 5531 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4277 7169 8379 4277 Size: 2892 1210 5175 Fragments arranged by size: 5175 2892 1210 BclI T'GATC_A Cuts at: 1548 5518 1548 Size: 3970 5307 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3353 3608 7888 Size: 107 122 71 631 605 1681 255 4280 Cuts at: 7888 136 Size: 1525 Fragments arranged by size: 4280 1681 1525 631 605 255 122 107 71 BglII A'GATC_T Cuts at: 3924 9272 3924 Size: 5348 3929 BlpI GC'TnA_GC Cuts at: 2453 2690 3186 5054 6694 2453 Size: 237 496 1868 1640 5036 Fragments arranged by size: 5036 1868 1640 496 237 Bme1580I G_kGCm'C Cuts at: 1790 2651 3485 3847 3903 4486 5326 6280 7338 Size: 861 834 362 56 583 840 954 1058 Cuts at: 7338 8494 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1058 954 861 840 834 583 362 56 BmgBI CAC'GTC Cuts at: 1318 2579 6031 1318 Size: 1261 3452 4564 Fragments arranged by size: 4564 3452 1261 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2500 6352 7008 Size: 416 53 83 399 24 1083 3852 656 Cuts at: 7008 7966 442 Size: 958 1753 Fragments arranged by size: 3852 1753 1083 958 656 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9277 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2594 3533 3578 3941 4447 4682 5741 6107 Size: 1355 939 45 363 506 235 1059 366 Cuts at: 6107 7937 1239 Size: 1830 2579 Fragments arranged by size: 2579 1830 1355 1059 939 506 366 363 235 45 Bpu10I CC'TnA_GC Cuts at: 2678 2748 3450 5825 6321 2678 Size: 70 702 2375 496 5634 Fragments arranged by size: 5634 2375 702 496 70 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2249 4805 5752 6515 7402 8267 8565 2249 Size: 2556 947 763 887 865 298 2961 Fragments arranged by size: 2961 2556 947 887 865 763 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7940 882 Size: 617 6441 2219 Fragments arranged by size: 6441 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3181 3543 6364 7068 493 Size: 711 1977 362 2821 704 2702 Fragments arranged by size: 2821 2702 1977 711 704 362 BsaBI GATnn'nnATC Cuts at: 5039 9271 5039 Size: 4232 5045 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5335 6459 7084 7466 Size: 53 83 186 936 3802 1124 625 382 Cuts at: 7466 8101 275 Size: 635 1451 Fragments arranged by size: 3802 1451 1124 936 635 625 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4077 7710 8108 8382 8529 8940 4077 Size: 3633 398 274 147 411 4414 Fragments arranged by size: 4414 3633 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2484 2514 1178 Size: 30 165 30 1081 30 7941 Fragments arranged by size: 7941 1081 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2252 2745 3044 3679 4620 4795 4798 5036 Size: 1066 493 299 635 941 175 3 238 Cuts at: 5036 6020 6338 1186 Size: 984 318 4125 Fragments arranged by size: 4125 1066 984 941 635 493 318 299 238 175 3 BseYI C'CCAG_C Cuts at: 4485 5230 5245 8480 8976 4485 Size: 745 15 3235 496 4786 Fragments arranged by size: 4786 3235 745 496 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2898 3626 3764 5816 5970 6098 1163 Size: 573 1162 728 138 2052 154 128 4342 Fragments arranged by size: 4342 2052 1162 728 573 154 138 128 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6576 6727 7488 8516 1540 Size: 275 186 4575 151 761 1028 2301 Fragments arranged by size: 4575 2301 1028 761 275 186 151 BsiHKAI G_wGCw'C Cuts at: 3741 3825 4879 5067 6126 6280 6516 7338 7423 Size: 84 1054 188 1059 154 236 822 85 Cuts at: 7423 8494 3741 Size: 1071 4524 Fragments arranged by size: 4524 1071 1059 1054 822 236 188 154 85 84 BsmI GAATG_Cn' Cuts at: 4467 5087 6788 6881 4467 Size: 620 1701 93 6863 Fragments arranged by size: 6863 1701 620 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2651 2778 3485 3741 3825 3847 3903 Size: 399 861 127 707 256 84 22 56 Cuts at: 3903 4486 4879 5067 5326 6126 6280 6516 6596 Size: 583 393 188 259 800 154 236 80 Cuts at: 6596 7338 7423 8494 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 861 800 742 707 583 399 393 259 256 236 188 154 127 85 84 80 56 22 BspEI T'CCGG_A Cuts at: 8940 8940 Size: 9277 BspHI T'CATG_A Cuts at: 7166 8896 7166 Size: 1730 7547 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2410 2554 2613 2674 3030 3300 4047 5263 Size: 1566 144 59 61 356 270 747 1216 Cuts at: 5263 6264 6632 844 Size: 1001 368 3489 Fragments arranged by size: 3489 1566 1216 1001 747 368 356 270 144 61 59 BsrBI CCG'CTC Cuts at: 1394 1823 7164 1394 Size: 429 5341 3507 Fragments arranged by size: 5341 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 7772 7946 65 Size: 1183 6524 174 1396 Fragments arranged by size: 6524 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3220 7921 8108 1700 Size: 110 1410 4701 187 2869 Fragments arranged by size: 4701 2869 1410 187 110 BsrGI T'GTAC_A Cuts at: 96 6297 96 Size: 6201 3076 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9277 BssSI C'ACGA_G Cuts at: 3740 6315 7337 8349 3740 Size: 2575 1022 1012 4668 Fragments arranged by size: 4668 2575 1022 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3469 3751 5278 6121 794 Size: 2675 282 1527 843 3950 Fragments arranged by size: 3950 2675 1527 843 282 BstBI TT'CG_AA Cuts at: 3334 8081 3334 Size: 4747 4530 BstEII G'GTnAC_C Cuts at: 1184 6382 1184 Size: 5198 4079 BstXI CCAn_nnnn'nTGG Cuts at: 2914 3795 4850 2914 Size: 881 1055 7341 Fragments arranged by size: 7341 1055 881 BstYI r'GATC_y Cuts at: 1972 2217 2345 3531 3924 5638 6607 6974 7359 Size: 245 128 1186 393 1714 969 367 385 Cuts at: 7359 7376 8828 8914 8926 9272 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1714 1452 1186 969 393 385 367 346 245 128 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2627 6150 2627 Size: 3523 5754 BtgI C'CryG_G Cuts at: 513 1065 2004 2835 3404 3489 4317 4773 513 Size: 552 939 831 569 85 828 456 5017 Fragments arranged by size: 5017 939 831 828 569 552 456 85 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3112 4125 6292 487 Size: 683 56 1886 1013 2167 3472 Fragments arranged by size: 3472 2167 1886 1013 683 56 BtsI GCAGTG_nn' Cuts at: 3643 5395 5753 5819 5987 6146 6793 7587 7607 Size: 1752 358 66 168 159 647 794 20 Cuts at: 7607 3643 Size: 5313 Fragments arranged by size: 5313 1752 794 647 358 168 159 66 20 ClaI AT'CG_AT Cuts at: 1554 3312 1554 Size: 1758 7519 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9242 DraI TTT'AAA Cuts at: 6581 6581 Size: 9277 DraIII CAC_nnn'GTG Cuts at: 5852 6121 5852 Size: 269 9008 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8284 809 Size: 507 6968 1802 Fragments arranged by size: 6968 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2424 2536 2665 Size: 54 1425 50 275 18 594 112 129 Cuts at: 2665 2904 3105 4177 4290 4776 6573 6724 7613 Size: 239 201 1072 113 486 1797 151 889 Cuts at: 7613 9242 8 Size: 1629 43 Fragments arranged by size: 1797 1629 1425 1072 889 594 486 275 239 201 151 129 113 112 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6573 6724 1537 Size: 275 4761 151 4090 Fragments arranged by size: 4761 4090 275 151 EarI CTCTTCn'nnn_ Cuts at: 1353 3147 3411 4344 5620 6131 7207 1353 Size: 1794 264 933 1276 511 1076 3423 Fragments arranged by size: 3423 1794 1276 1076 933 511 264 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7864 8238 8384 387 Size: 1064 138 6275 374 146 1280 Fragments arranged by size: 6275 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3739 6514 3739 Size: 2775 6502 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2273 2594 3260 3285 3533 3578 Size: 393 578 63 321 666 25 248 45 Cuts at: 3578 3941 4104 4447 4682 5741 6028 6098 6107 Size: 363 163 343 235 1059 287 70 9 Cuts at: 6107 7340 7937 8724 8992 1239 Size: 1233 597 787 268 1524 Fragments arranged by size: 1524 1233 1059 787 666 597 578 393 363 343 321 287 268 248 235 163 70 63 45 25 9 EcoNI CCTnn'n_nnAGG Cuts at: 3026 5465 3026 Size: 2439 6838 EcoO109I rG'GnC_Cy Cuts at: 3482 3900 5207 5242 5322 3482 Size: 418 1307 35 80 7437 Fragments arranged by size: 7437 1307 418 80 35 EcoRI G'AATT_C Cuts at: 3141 4991 6586 6964 3141 Size: 1850 1595 378 5454 Fragments arranged by size: 5454 1850 1595 378 EcoRV GAT'ATC Cuts at: 3252 3252 Size: 9277 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2153 4406 4663 5074 6403 2153 Size: 2253 257 411 1329 5027 Fragments arranged by size: 5027 2253 1329 411 257 FspI TGC'GCA Cuts at: 7783 7783 Size: 9277 HaeII r_GCGC'y Cuts at: 1678 3359 5338 8424 1678 Size: 1681 1979 3086 2531 Fragments arranged by size: 3086 2531 1979 1681 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2269 2301 2741 2773 3424 Size: 32 135 32 697 32 440 32 651 Cuts at: 3424 3456 3530 3562 4403 4435 4960 4992 5199 Size: 32 74 32 841 32 525 32 207 Cuts at: 5199 5231 5754 5786 6376 6408 7918 7950 7992 Size: 32 523 32 590 32 1510 32 42 Cuts at: 7992 8024 1373 Size: 32 2626 Fragments arranged by size: 2626 1510 841 697 651 590 525 523 440 207 135 74 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2397 2569 6621 6867 669 Size: 988 281 459 172 4052 246 3079 Fragments arranged by size: 4052 3079 988 459 281 246 172 HindIII A'AGCT_T Cuts at: 748 2036 2180 4144 5925 748 Size: 1288 144 1964 1781 4100 Fragments arranged by size: 4100 1964 1781 1288 144 HpaI GTT'AAC Cuts at: 6867 6867 Size: 9277 KasI G'GCGC_C Cuts at: 5334 5334 Size: 9277 KpnI G_GTAC'C Cuts at: 6602 6602 Size: 9277 MfeI C'AATT_G Cuts at: 6876 6876 Size: 9277 MluI A'CGCG_T Cuts at: 9153 9153 Size: 9277 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3418 4656 5472 6348 8391 8575 1305 Size: 2113 1238 816 876 2043 184 2007 Fragments arranged by size: 2113 2043 2007 1238 876 816 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2538 2667 2906 3107 4179 Size: 54 1425 343 706 129 239 201 1072 Cuts at: 4179 4292 10 Size: 113 4995 Fragments arranged by size: 4995 1425 1072 706 343 239 201 129 113 54 MslI CAynn'nnrTG Cuts at: 518 1166 4547 5045 5702 7235 7594 7753 518 Size: 648 3381 498 657 1533 359 159 2042 Fragments arranged by size: 3381 2042 1533 657 648 498 359 159 MspA1I CmG'CkG Cuts at: 1606 2677 3234 3446 4033 4660 4720 5813 6085 Size: 1071 557 212 587 627 60 1093 272 Cuts at: 6085 6398 6693 7370 8518 8763 1606 Size: 313 295 677 1148 245 2120 Fragments arranged by size: 2120 1148 1093 1071 677 627 587 557 313 295 272 245 212 60 NarI GG'CG_CC Cuts at: 5335 5335 Size: 9277 NcoI C'CATG_G Cuts at: 513 1065 2004 2835 4317 4773 513 Size: 552 939 831 1482 456 5017 Fragments arranged by size: 5017 1482 939 831 552 456 NdeI CA'TA_TG Cuts at: 387 387 Size: 9277 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9277 NotI GC'GGCC_GC Cuts at: 6724 6724 Size: 9277 NspI r_CATG'y Cuts at: 1132 1229 3507 5782 6635 8180 1132 Size: 97 2278 2275 853 1545 2229 Fragments arranged by size: 2278 2275 2229 1545 853 97 PasI CC'CwG_GG Cuts at: 1348 5155 1348 Size: 3807 5470 PciI A'CATG_T Cuts at: 5778 8176 5778 Size: 2398 6879 PflMI CCAn_nnn'nTGG Cuts at: 1108 5203 5764 5852 1108 Size: 4095 561 88 4533 Fragments arranged by size: 4533 4095 561 88 PfoI T'CCnGG_A Cuts at: 3510 3534 3765 6435 3510 Size: 24 231 2670 6352 Fragments arranged by size: 6352 2670 231 24 PmeI GTTT'AAAC Cuts at: 6581 6581 Size: 9277 PmlI CAC'GTG Cuts at: 3181 3543 3181 Size: 362 8915 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2708 2740 3146 3178 7346 7378 8859 8891 2708 Size: 32 406 32 4168 32 1481 32 3094 Fragments arranged by size: 4168 3094 1481 406 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5207 5207 Size: 9277 PshAI GACnn'nnGTC Cuts at: 1613 4497 5388 1613 Size: 2884 891 5502 Fragments arranged by size: 5502 2884 891 PsiI TTA'TAA Cuts at: 2172 6847 2172 Size: 4675 4602 PspOMI G'GGCC_C Cuts at: 3481 3843 3899 5322 3481 Size: 362 56 1423 7436 Fragments arranged by size: 7436 1423 362 56 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2118 2150 2118 Size: 32 9245 PstI C_TGCA'G Cuts at: 830 2480 2559 3027 3804 4044 5271 5286 5304 Size: 1650 79 468 777 240 1227 15 18 Cuts at: 5304 5402 6141 6417 6486 6629 830 Size: 98 739 276 69 143 3478 Fragments arranged by size: 3478 1650 1227 777 739 468 276 240 143 98 79 69 18 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9277 PvuII CAG'CTG Cuts at: 2677 3446 4033 4660 4720 5813 6398 2677 Size: 769 587 627 60 1093 585 5556 Fragments arranged by size: 5556 1093 769 627 587 585 60 SacI G_AGCT'C Cuts at: 3741 6516 3741 Size: 2775 6502 SalI G'TCGA_C Cuts at: 1936 2395 6619 1936 Size: 459 4224 4594 Fragments arranged by size: 4594 4224 459 SapI GCTCTTCn'nnn_ Cuts at: 6131 6131 Size: 9277 SbfI CC_TGCA'GG Cuts at: 2480 3027 6629 2480 Size: 547 3602 5128 Fragments arranged by size: 5128 3602 547 ScaI AGT'ACT Cuts at: 7525 9237 7525 Size: 1712 7565 SexAI A'CCwGG_T Cuts at: 5186 5186 Size: 9277 SfcI C'TryA_G Cuts at: 826 1045 2476 2555 3023 3800 3874 4040 4212 Size: 219 1431 79 468 777 74 166 172 Cuts at: 4212 5267 5282 5300 5398 6137 6413 6482 6625 Size: 1055 15 18 98 739 276 69 143 Cuts at: 6625 7760 8441 8632 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1431 1135 1055 777 739 681 468 276 219 191 172 166 143 98 79 74 69 18 15 SfoI GGC'GCC Cuts at: 5336 5336 Size: 9277 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9277 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9277 SmaI CCC'GGG Cuts at: 1872 5327 6604 1872 Size: 3455 1277 4545 Fragments arranged by size: 4545 3455 1277 SmlI C'TyrA_G Cuts at: 820 1017 1943 2033 2264 4820 5731 6530 7381 Size: 197 926 90 231 2556 911 799 851 Cuts at: 7381 8282 8544 820 Size: 901 262 1553 Fragments arranged by size: 2556 1553 926 911 901 851 799 262 231 197 90 SnaBI TAC'GTA Cuts at: 493 7068 493 Size: 6575 2702 SpeI A'CTAG_T Cuts at: 152 4070 152 Size: 3918 5359 SphI G_CATG'C Cuts at: 1132 3507 6635 1132 Size: 2375 3128 3774 Fragments arranged by size: 3774 3128 2375 SspI AAT'ATT Cuts at: 5 52 7201 5 Size: 47 7149 2081 Fragments arranged by size: 7149 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9277 StyI C'CwwG_G Cuts at: 513 1065 2004 2533 2835 4317 4773 5425 6716 Size: 552 939 529 302 1482 456 652 1291 Cuts at: 6716 513 Size: 3074 Fragments arranged by size: 3074 1482 1291 939 652 552 529 456 302 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3229 5335 5714 7304 7321 7474 7659 Size: 1255 1649 2106 379 1590 17 153 185 Cuts at: 7659 8104 325 Size: 445 1498 Fragments arranged by size: 2106 1649 1590 1498 1255 445 379 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4924 6297 7523 Size: 274 80 33 51 1443 2947 1373 1226 Cuts at: 7523 9235 96 Size: 1712 138 Fragments arranged by size: 2947 1712 1443 1373 1226 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4180 4212 9237 9269 4180 Size: 32 5025 32 4188 Fragments arranged by size: 5025 4188 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9277 XbaI T'CTAG_A Cuts at: 2938 6613 2938 Size: 3675 5602 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3073 3073 Size: 9277 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9277 XmaI C'CCGG_G Cuts at: 1870 5325 6602 1870 Size: 3455 1277 4545 Fragments arranged by size: 4545 3455 1277 XmnI GAAnn'nnTTC Cuts at: 6188 7406 6188 Size: 1218 8059 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6460 7085 276 Size: 53 83 186 936 4926 625 2468 Fragments arranged by size: 4926 2468 936 625 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BfrBI BplI BsiWI BsmBI BstZ17I FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI RsrII SacII SanDI SfiI SrfI SwaI