(Circular) (Six-base) MAPSORT of: pFN21AB8963.seq Check: 3720 from: 1 to: 5755 pFN21AB8963 5755 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3565 278 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3098 1937 Size: 1161 4594 Acc65I G'GTAC_C Cuts at: 3076 3076 Size: 5755 AclI AA'CG_TT Cuts at: 1563 3882 4255 1563 Size: 2319 373 3063 Fragments arranged by size: 3063 2319 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2689 3818 5202 5470 1632 Size: 1057 1129 1384 268 1917 Fragments arranged by size: 1917 1384 1129 1057 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 4654 5631 1203 Size: 3451 977 1327 Fragments arranged by size: 3451 1327 977 AgeI A'CCGG_T Cuts at: 4586 4586 Size: 5755 AhdI GACnn_n'nnGTC Cuts at: 4484 4484 Size: 5755 AleI CACnn'nnGTG Cuts at: 1166 2117 1166 Size: 951 4804 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2042 1184 Size: 858 4897 AlwNI CAG_nnn'CTG Cuts at: 3498 5070 3498 Size: 1572 4183 ApaLI G'TGCA_C Cuts at: 2533 3812 4968 2533 Size: 1279 1156 3320 Fragments arranged by size: 3320 1279 1156 ApoI r'AATT_y Cuts at: 1493 2710 2905 3064 3290 3442 5551 1493 Size: 1217 195 159 226 152 2109 1697 Fragments arranged by size: 2109 1697 1217 226 195 159 152 AseI AT'TA_AT Cuts at: 160 1032 4309 160 Size: 872 3277 1606 Fragments arranged by size: 3277 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 2518 3080 1870 Size: 73 575 562 4545 Fragments arranged by size: 4545 575 562 73 AvrII C'CTAG_G Cuts at: 2813 2813 Size: 5755 BamHI G'GATC_C Cuts at: 2637 2817 3085 3452 2637 Size: 180 268 367 4940 Fragments arranged by size: 4940 367 268 180 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2809 3076 4531 618 Size: 325 217 627 1022 267 1455 1842 Fragments arranged by size: 1842 1455 1022 627 325 267 217 BanII G_rGCy'C Cuts at: 1391 2761 3074 1391 Size: 1370 313 4072 Fragments arranged by size: 4072 1370 313 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4784 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3946 3980 1903 Size: 34 50 34 1925 34 3678 Fragments arranged by size: 3678 1925 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3647 4857 3647 Size: 1210 4545 BclI T'GATC_A Cuts at: 1548 2604 1548 Size: 1056 4699 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2077 4366 136 Size: 107 122 71 631 605 405 2289 1525 Fragments arranged by size: 2289 1525 631 605 405 122 107 71 BglII A'GATC_T Cuts at: 5750 5750 Size: 5755 BlpI GC'TnA_GC Cuts at: 2209 3172 2209 Size: 963 4792 Bme1580I G_kGCm'C Cuts at: 1790 2537 3816 4972 1790 Size: 747 1279 1156 2573 Fragments arranged by size: 2573 1279 1156 747 BmgBI CAC'GTC Cuts at: 1318 2983 1318 Size: 1665 4090 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2539 3486 4444 Size: 416 53 83 399 24 1122 947 958 Cuts at: 4444 442 Size: 1753 Fragments arranged by size: 1753 1122 958 947 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2492 2633 4415 1239 Size: 1253 141 1782 2579 Fragments arranged by size: 2579 1782 1253 141 Bpu10I CC'TnA_GC Cuts at: 2177 2330 2177 Size: 153 5602 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2387 3880 4745 5043 2387 Size: 1493 865 298 3099 Fragments arranged by size: 3099 1493 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4418 882 Size: 617 2919 2219 Fragments arranged by size: 2919 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2446 3546 493 Size: 711 1242 1100 2702 Fragments arranged by size: 2702 1242 1100 711 BsaBI GATnn'nnATC Cuts at: 5749 5749 Size: 5755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2084 3562 3944 4579 Size: 53 83 186 936 551 1478 382 635 Cuts at: 4579 275 Size: 1451 Fragments arranged by size: 1478 1451 936 635 551 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4188 4586 4860 5007 5418 4188 Size: 398 274 147 411 4525 Fragments arranged by size: 4525 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5530 Fragments arranged by size: 5530 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2441 2751 1186 Size: 1255 310 4190 Fragments arranged by size: 4190 1255 310 BseYI C'CCAG_C Cuts at: 2040 2754 4958 5454 2040 Size: 714 2204 496 2341 Fragments arranged by size: 2341 2204 714 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2282 2517 1163 Size: 573 546 235 4401 Fragments arranged by size: 4401 573 546 235 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2478 3205 3966 4994 1540 Size: 275 186 477 727 761 1028 2301 Fragments arranged by size: 2301 1028 761 727 477 275 186 BsiHKAI G_wGCw'C Cuts at: 2052 2451 2537 3816 3901 4972 2052 Size: 399 86 1279 85 1071 2835 Fragments arranged by size: 2835 1279 1071 399 86 85 BsmI GAATG_Cn' Cuts at: 3266 3359 3266 Size: 93 5662 BsmBI CGTCTCn'nnnn_ Cuts at: 3015 3015 Size: 5755 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2451 2537 2761 3074 3816 3901 Size: 399 262 399 86 224 313 742 85 Cuts at: 3901 4972 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 399 313 262 224 86 85 BspEI T'CCGG_A Cuts at: 5418 5418 Size: 5755 BspHI T'CATG_A Cuts at: 3644 5374 3644 Size: 1730 4025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3110 844 Size: 2266 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3642 1394 Size: 429 1819 3507 Fragments arranged by size: 3507 1819 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4250 4424 65 Size: 1183 3002 174 1396 Fragments arranged by size: 3002 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2360 4399 4586 1700 Size: 110 550 2039 187 2869 Fragments arranged by size: 2869 2039 550 187 110 BsrGI T'GTAC_A Cuts at: 96 2418 2945 96 Size: 2322 527 2906 Fragments arranged by size: 2906 2322 527 BssHII G'CGCG_C Cuts at: 1925 2022 2064 1925 Size: 97 42 5616 Fragments arranged by size: 5616 97 42 BssSI C'ACGA_G Cuts at: 2074 2530 3815 4827 2074 Size: 456 1285 1012 3002 Fragments arranged by size: 3002 1285 1012 456 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5755 BstBI TT'CG_AA Cuts at: 4559 4559 Size: 5755 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5755 BstXI CCAn_nnnn'nTGG Cuts at: 2122 2312 2575 2728 2122 Size: 190 263 153 5149 Fragments arranged by size: 5149 263 190 153 BstYI r'GATC_y Cuts at: 1972 2523 2637 2817 3085 3452 3837 3854 5306 Size: 551 114 180 268 367 385 17 1452 Cuts at: 5306 5392 5404 5750 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 551 385 367 346 268 180 114 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2053 2574 2784 513 Size: 552 988 521 210 3484 Fragments arranged by size: 3484 988 552 521 210 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2350 2554 487 Size: 683 56 1124 204 3688 Fragments arranged by size: 3688 1124 683 204 56 BtsI GCAGTG_nn' Cuts at: 2299 3271 4065 4085 2299 Size: 972 794 20 3969 Fragments arranged by size: 3969 972 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5720 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4762 809 Size: 507 3446 1802 Fragments arranged by size: 3446 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2232 2475 2781 Size: 54 1425 50 275 18 402 243 306 Cuts at: 2781 2838 2985 3202 4091 5720 8 Size: 57 147 217 889 1629 43 Fragments arranged by size: 1629 1425 889 402 306 275 243 217 147 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2475 3202 1537 Size: 275 663 727 4090 Fragments arranged by size: 4090 727 663 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3685 1353 Size: 2332 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2077 2548 4342 4716 4862 387 Size: 1064 138 488 471 1794 374 146 1280 Fragments arranged by size: 1794 1280 1064 488 471 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2492 2633 2689 3818 4415 5202 5470 Size: 393 860 141 56 1129 597 787 268 Cuts at: 5470 1239 Size: 1524 Fragments arranged by size: 1524 1129 860 787 597 393 268 141 56 EcoRI G'AATT_C Cuts at: 3064 3442 3064 Size: 378 5377 FspI TGC'GCA Cuts at: 4261 4261 Size: 5755 HaeII r_GCGC'y Cuts at: 1678 4902 1678 Size: 3224 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4396 4428 4470 4502 1373 Size: 32 135 32 2824 32 42 32 2626 Fragments arranged by size: 2824 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3099 3345 669 Size: 988 281 1161 246 3079 Fragments arranged by size: 3079 1161 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KpnI G_GTAC'C Cuts at: 3080 3080 Size: 5755 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4869 5053 1305 Size: 3564 184 2007 Fragments arranged by size: 3564 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2234 2840 10 Size: 54 1425 343 402 606 2925 Fragments arranged by size: 2925 1425 606 402 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2117 2120 2198 2573 2726 3713 4072 Size: 648 951 3 78 375 153 987 359 Cuts at: 4072 4231 518 Size: 159 2042 Fragments arranged by size: 2042 987 951 648 375 359 159 153 78 3 MspA1I CmG'CkG Cuts at: 1606 2055 2868 3171 3848 4996 5241 1606 Size: 449 813 303 677 1148 245 2120 Fragments arranged by size: 2120 1148 813 677 449 303 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5203 NdeI CA'TA_TG Cuts at: 387 2456 387 Size: 2069 3686 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5755 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 3113 4658 1132 Size: 97 1884 1545 2229 Fragments arranged by size: 2229 1884 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5755 PciI A'CATG_T Cuts at: 4654 4654 Size: 5755 PflMI CCAn_nnn'nTGG Cuts at: 1108 2754 1108 Size: 1646 4109 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 5337 5369 3824 Size: 32 1481 32 4210 Fragments arranged by size: 4210 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5755 PsiI TTA'TAA Cuts at: 3325 3325 Size: 5755 PstI C_TGCA'G Cuts at: 830 3107 830 Size: 2277 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 PvuII CAG'CTG Cuts at: 2868 2868 Size: 5755 SacII CC_GC'GG Cuts at: 2056 2056 Size: 5755 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 4003 5715 4003 Size: 1712 4043 SexAI A'CCwGG_T Cuts at: 2223 2223 Size: 5755 SfcI C'TryA_G Cuts at: 826 1045 3103 4238 4919 5110 826 Size: 219 2058 1135 681 191 1471 Fragments arranged by size: 2058 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5755 SmaI CCC'GGG Cuts at: 1872 3082 1872 Size: 1210 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2402 3859 4760 5022 820 Size: 197 926 459 1457 901 262 1553 Fragments arranged by size: 1553 1457 926 901 459 262 197 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 3113 1132 Size: 1981 3774 SspI AAT'ATT Cuts at: 5 52 3679 5 Size: 47 3627 2081 Fragments arranged by size: 3627 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5755 StyI C'CwwG_G Cuts at: 513 1065 2813 3194 513 Size: 552 1748 381 3074 Fragments arranged by size: 3074 1748 552 381 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3782 3799 3952 4137 4582 325 Size: 1255 2202 17 153 185 445 1498 Fragments arranged by size: 2202 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2418 2945 4001 Size: 274 80 33 51 1443 441 527 1056 Cuts at: 4001 5713 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1056 527 441 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5715 5747 5715 Size: 32 5723 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5755 XbaI T'CTAG_A Cuts at: 3091 3091 Size: 5755 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2575 2848 2575 Size: 273 5482 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5755 XmaI C'CCGG_G Cuts at: 1870 3080 1870 Size: 1210 4545 XmnI GAAnn'nnTTC Cuts at: 3884 3884 Size: 5755 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3563 276 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI