(Circular) (Six-base) MAPSORT of: pFN21AB9746.seq Check: 2520 from: 1 to: 6331 pFN21AB9746 6331 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4141 278 Size: 53 83 186 936 2605 2468 Fragments arranged by size: 2605 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2107 3674 1937 Size: 170 1567 4594 Fragments arranged by size: 4594 1567 170 Acc65I G'GTAC_C Cuts at: 3652 3652 Size: 6331 AclI AA'CG_TT Cuts at: 1563 4458 4831 1563 Size: 2895 373 3063 Fragments arranged by size: 3063 2895 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2551 2887 3139 3307 4394 5778 6046 1632 Size: 919 336 252 168 1087 1384 268 1917 Fragments arranged by size: 1917 1384 1087 919 336 268 252 168 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6134 AflIII A'CryG_T Cuts at: 1203 5230 6207 1203 Size: 4027 977 1327 Fragments arranged by size: 4027 1327 977 AgeI A'CCGG_T Cuts at: 5162 5162 Size: 6331 AhdI GACnn_n'nnGTC Cuts at: 2148 3240 5060 2148 Size: 1092 1820 3419 Fragments arranged by size: 3419 1820 1092 AleI CACnn'nnGTG Cuts at: 1166 2358 2526 3450 3534 1166 Size: 1192 168 924 84 3963 Fragments arranged by size: 3963 1192 924 168 84 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6331 AlwNI CAG_nnn'CTG Cuts at: 4074 5646 4074 Size: 1572 4759 ApaLI G'TGCA_C Cuts at: 3161 4388 5544 3161 Size: 1227 1156 3948 Fragments arranged by size: 3948 1227 1156 ApoI r'AATT_y Cuts at: 1493 2349 2601 2685 2853 3189 3357 3640 3866 Size: 856 252 84 168 336 168 283 226 Cuts at: 3866 4018 6127 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 856 336 283 252 226 168 168 152 84 AseI AT'TA_AT Cuts at: 160 1032 4885 160 Size: 872 3853 1606 Fragments arranged by size: 3853 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6331 AvaI C'yCGr_G Cuts at: 1870 1943 3656 1870 Size: 73 1713 4545 Fragments arranged by size: 4545 1713 73 BamHI G'GATC_C Cuts at: 3021 3661 4028 3021 Size: 640 367 5324 Fragments arranged by size: 5324 640 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3652 5107 618 Size: 325 217 627 1865 1455 1842 Fragments arranged by size: 1865 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3650 1391 Size: 2259 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2941 3575 928 Size: 971 1042 634 3684 Fragments arranged by size: 3684 1042 971 634 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4522 4556 1903 Size: 34 2585 34 3678 Fragments arranged by size: 3678 2585 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4223 5433 4223 Size: 1210 5121 BclI T'GATC_A Cuts at: 1548 1548 Size: 6331 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4942 136 Size: 107 122 71 631 605 3270 1525 Fragments arranged by size: 3270 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6326 6326 Size: 6331 BlpI GC'TnA_GC Cuts at: 2326 2673 3748 2326 Size: 347 1075 4909 Fragments arranged by size: 4909 1075 347 Bme1580I G_kGCm'C Cuts at: 1790 3165 4392 5548 1790 Size: 1375 1227 1156 2573 Fragments arranged by size: 2573 1375 1227 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6331 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2451 3039 3375 Size: 416 53 83 399 24 1034 588 336 Cuts at: 3375 4062 5020 442 Size: 687 958 1753 Fragments arranged by size: 1753 1034 958 687 588 416 399 336 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6331 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3135 4991 1239 Size: 1896 1856 2579 Fragments arranged by size: 2579 1896 1856 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4456 5321 5619 4456 Size: 865 298 5168 Fragments arranged by size: 5168 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4994 882 Size: 617 3495 2219 Fragments arranged by size: 3495 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2120 3166 4122 493 Size: 711 916 1046 956 2702 Fragments arranged by size: 2702 1046 956 916 711 BsaBI GATnn'nnATC Cuts at: 6325 6325 Size: 6331 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4138 4520 5155 275 Size: 53 83 186 936 2605 382 635 1451 Fragments arranged by size: 2605 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4764 5162 5436 5583 5994 4764 Size: 398 274 147 411 5101 Fragments arranged by size: 5101 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6106 Fragments arranged by size: 6106 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2564 1186 Size: 1378 4953 BseYI C'CCAG_C Cuts at: 5534 6030 5534 Size: 496 5835 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5758 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3781 4542 5570 1540 Size: 275 186 1780 761 1028 2301 Fragments arranged by size: 2301 1780 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3165 4392 4477 5548 3165 Size: 1227 85 1071 3948 Fragments arranged by size: 3948 1227 1071 85 BsmI GAATG_Cn' Cuts at: 2633 2894 3842 3935 2633 Size: 261 948 93 5029 Fragments arranged by size: 5029 948 261 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3165 3650 4392 4477 5548 1391 Size: 399 1375 485 742 85 1071 2174 Fragments arranged by size: 2174 1375 1071 742 485 399 85 BspEI T'CCGG_A Cuts at: 5994 5994 Size: 6331 BspHI T'CATG_A Cuts at: 4220 5950 4220 Size: 1730 4601 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3686 844 Size: 2842 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4218 1394 Size: 429 2395 3507 Fragments arranged by size: 3507 2395 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2390 4826 5000 65 Size: 1183 1142 2436 174 1396 Fragments arranged by size: 2436 1396 1183 1142 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4975 5162 1700 Size: 110 3165 187 2869 Fragments arranged by size: 3165 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3259 96 Size: 3163 3168 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6331 BssSI C'ACGA_G Cuts at: 4391 5403 4391 Size: 1012 5319 BstAPI GCAn_nnn'nTGC Cuts at: 794 2889 794 Size: 2095 4236 BstBI TT'CG_AA Cuts at: 5135 5135 Size: 6331 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6331 BstYI r'GATC_y Cuts at: 1972 3021 3661 4028 4413 4430 5882 5968 5980 Size: 1049 640 367 385 17 1452 86 12 Cuts at: 5980 6326 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1049 640 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4840 Fragments arranged by size: 4840 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5592 Fragments arranged by size: 5592 683 56 BtsI GCAGTG_nn' Cuts at: 3847 4641 4661 3847 Size: 794 20 5517 Fragments arranged by size: 5517 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6331 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2620 2655 3544 3579 529 Size: 35 2056 35 889 35 3281 Fragments arranged by size: 3281 2056 889 35 35 35 DraI TTT'AAA Cuts at: 3550 3635 3550 Size: 85 6246 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5338 809 Size: 507 4022 1802 Fragments arranged by size: 4022 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3778 4667 6296 Size: 54 1425 50 275 18 1948 889 1629 Cuts at: 6296 8 Size: 43 Fragments arranged by size: 1948 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3778 1537 Size: 275 1966 4090 Fragments arranged by size: 4090 1966 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4261 1353 Size: 2908 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4918 5292 5438 387 Size: 1064 138 3329 374 146 1280 Fragments arranged by size: 3329 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2551 2887 3135 3139 3307 4394 4991 Size: 393 919 336 248 4 168 1087 597 Cuts at: 4991 5778 6046 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1087 919 787 597 393 336 268 248 168 4 EcoRI G'AATT_C Cuts at: 2349 2601 2685 2853 3357 3640 4018 2349 Size: 252 84 168 504 283 378 4662 Fragments arranged by size: 4662 504 378 283 252 168 84 FspI TGC'GCA Cuts at: 4837 4837 Size: 6331 HaeII r_GCGC'y Cuts at: 1678 5478 1678 Size: 3800 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2134 2166 2232 2264 3226 Size: 32 135 32 562 32 66 32 962 Cuts at: 3226 3258 4972 5004 5046 5078 1373 Size: 32 1714 32 42 32 2626 Fragments arranged by size: 2626 1714 962 562 135 66 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3675 3921 669 Size: 988 281 1737 246 3079 Fragments arranged by size: 3079 1737 988 281 246 HindIII A'AGCT_T Cuts at: 748 2478 2498 748 Size: 1730 20 4581 Fragments arranged by size: 4581 1730 20 HpaI GTT'AAC Cuts at: 3921 3921 Size: 6331 KpnI G_GTAC'C Cuts at: 3656 3656 Size: 6331 MfeI C'AATT_G Cuts at: 3930 3930 Size: 6331 MluI A'CGCG_T Cuts at: 6207 6207 Size: 6331 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2007 5445 5629 1305 Size: 702 3438 184 2007 Fragments arranged by size: 3438 2007 702 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4509 Fragments arranged by size: 4509 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2122 2190 2274 2358 2374 2458 2526 Size: 648 956 68 84 84 16 84 68 Cuts at: 2526 2610 2778 3198 3438 3450 3534 3618 4289 Size: 84 168 420 240 12 84 84 671 Cuts at: 4289 4648 4807 518 Size: 359 159 2042 Fragments arranged by size: 2042 956 671 648 420 359 240 168 159 84 84 84 84 84 84 68 68 16 12 MspA1I CmG'CkG Cuts at: 1606 3523 3747 4424 5572 5817 1606 Size: 1917 224 677 1148 245 2120 Fragments arranged by size: 2120 1917 1148 677 245 224 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4840 Fragments arranged by size: 4840 939 552 NdeI CA'TA_TG Cuts at: 387 2455 3615 387 Size: 2068 1160 3103 Fragments arranged by size: 3103 2068 1160 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6331 NotI GC'GGCC_GC Cuts at: 3778 3778 Size: 6331 NspI r_CATG'y Cuts at: 1132 1229 2273 3689 5234 1132 Size: 97 1044 1416 1545 2229 Fragments arranged by size: 2229 1545 1416 1044 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6331 PciI A'CATG_T Cuts at: 5230 5230 Size: 6331 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6331 PmeI GTTT'AAAC Cuts at: 3635 3635 Size: 6331 PmlI CAC'GTG Cuts at: 2120 2120 Size: 6331 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4400 4432 5913 5945 4400 Size: 32 1481 32 4786 Fragments arranged by size: 4786 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6331 PsiI TTA'TAA Cuts at: 3901 3901 Size: 6331 PstI C_TGCA'G Cuts at: 830 3683 830 Size: 2853 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6331 SalI G'TCGA_C Cuts at: 1936 3673 1936 Size: 1737 4594 SbfI CC_TGCA'GG Cuts at: 3683 3683 Size: 6331 ScaI AGT'ACT Cuts at: 2518 2992 4579 6291 2518 Size: 474 1587 1712 2558 Fragments arranged by size: 2558 1712 1587 474 SexAI A'CCwGG_T Cuts at: 2583 2583 Size: 6331 SfcI C'TryA_G Cuts at: 826 1045 3679 4814 5495 5686 826 Size: 219 2634 1135 681 191 1471 Fragments arranged by size: 2634 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6331 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6331 SmaI CCC'GGG Cuts at: 1872 3658 1872 Size: 1786 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4435 5336 5598 820 Size: 197 926 2492 901 262 1553 Fragments arranged by size: 2492 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4122 493 Size: 3629 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6331 SphI G_CATG'C Cuts at: 1132 3689 1132 Size: 2557 3774 SspI AAT'ATT Cuts at: 5 52 4255 5 Size: 47 4203 2081 Fragments arranged by size: 4203 2081 47 StuI AGG'CCT Cuts at: 1517 2312 2564 2648 2816 2984 3152 3320 3488 Size: 795 252 84 168 168 168 168 168 Cuts at: 3488 1517 Size: 4360 Fragments arranged by size: 4360 795 252 168 168 168 168 168 84 StyI C'CwwG_G Cuts at: 513 1065 2004 3770 513 Size: 552 939 1766 3074 Fragments arranged by size: 3074 1766 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2532 4358 4375 4528 4713 5158 325 Size: 1255 952 1826 17 153 185 445 1498 Fragments arranged by size: 1826 1498 1255 952 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2422 2516 2990 Size: 274 80 33 51 1443 445 94 474 Cuts at: 2990 3259 4577 6289 96 Size: 269 1318 1712 138 Fragments arranged by size: 1712 1443 1318 474 445 274 269 138 94 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6291 6323 6291 Size: 32 6299 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6331 XbaI T'CTAG_A Cuts at: 3667 3667 Size: 6331 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6331 XmaI C'CCGG_G Cuts at: 1870 3656 1870 Size: 1786 4545 XmnI GAAnn'nnTTC Cuts at: 2564 2732 2900 4460 2564 Size: 168 168 1560 4435 Fragments arranged by size: 4435 1560 168 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4139 276 Size: 53 83 186 936 2605 2468 Fragments arranged by size: 2605 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI