(Circular) (Six-base) MAPSORT of: pFN21AA2033.seq Check: 1722 from: 1 to: 6943 pFN21AA2033 6943 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2852 3382 3860 4753 Size: 53 83 186 936 1316 530 478 893 Cuts at: 4753 278 Size: 2468 Fragments arranged by size: 2468 1316 936 893 530 478 186 83 53 AccI GT'mk_AC Cuts at: 1937 2872 3208 3376 3628 3712 3880 4216 4286 Size: 935 336 168 252 84 168 336 70 Cuts at: 4286 1937 Size: 4594 Fragments arranged by size: 4594 935 336 336 252 168 168 84 70 Acc65I G'GTAC_C Cuts at: 4264 4264 Size: 6943 AclI AA'CG_TT Cuts at: 1563 5070 5443 1563 Size: 3507 373 3063 Fragments arranged by size: 3507 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2824 2988 3324 3832 5006 6390 6658 1632 Size: 1192 164 336 508 1174 1384 268 1917 Fragments arranged by size: 1917 1384 1192 1174 508 336 268 164 AflII C'TTAA_G Cuts at: 820 1017 2692 820 Size: 197 1675 5071 Fragments arranged by size: 5071 1675 197 AflIII A'CryG_T Cuts at: 1203 5842 6819 1203 Size: 4639 977 1327 Fragments arranged by size: 4639 1327 977 AgeI A'CCGG_T Cuts at: 5774 5774 Size: 6943 AhdI GACnn_n'nnGTC Cuts at: 2841 3093 3177 3513 3849 4017 4185 5672 2841 Size: 252 84 336 336 168 168 1487 4112 Fragments arranged by size: 4112 1487 336 336 252 168 168 84 AleI CACnn'nnGTG Cuts at: 1166 2799 1166 Size: 1633 5310 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6943 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2123 2155 2123 Size: 32 6911 AlwNI CAG_nnn'CTG Cuts at: 4686 6258 4686 Size: 1572 5371 ApaLI G'TGCA_C Cuts at: 5000 6156 5000 Size: 1156 5787 ApoI r'AATT_y Cuts at: 1493 2051 2297 2541 2596 3274 3294 3462 3798 Size: 558 246 244 55 678 20 168 336 Cuts at: 3798 3966 4252 4478 4630 6739 1493 Size: 168 286 226 152 2109 1697 Fragments arranged by size: 2109 1697 678 558 336 286 246 244 226 168 168 152 55 20 AseI AT'TA_AT Cuts at: 160 1032 5497 160 Size: 872 4465 1606 Fragments arranged by size: 4465 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6943 AvaI C'yCGr_G Cuts at: 1870 1943 4268 1870 Size: 73 2325 4545 Fragments arranged by size: 4545 2325 73 BamHI G'GATC_C Cuts at: 4273 4640 4273 Size: 367 6576 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4264 5719 618 Size: 325 217 627 2477 1455 1842 Fragments arranged by size: 2477 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4262 1391 Size: 2871 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3312 928 Size: 971 1413 4559 Fragments arranged by size: 4559 1413 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5134 5168 1903 Size: 34 3197 34 3678 Fragments arranged by size: 3678 3197 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4835 6045 4835 Size: 1210 5733 BclI T'GATC_A Cuts at: 1548 2383 1548 Size: 835 6108 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5554 136 Size: 107 122 71 631 605 3882 1525 Fragments arranged by size: 3882 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6938 6938 Size: 6943 BlpI GC'TnA_GC Cuts at: 4360 4360 Size: 6943 Bme1580I G_kGCm'C Cuts at: 1790 5004 6160 1790 Size: 3214 1156 2573 Fragments arranged by size: 3214 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2110 1318 Size: 792 6151 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4674 5632 442 Size: 416 53 83 399 24 3257 958 1753 Fragments arranged by size: 3257 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6943 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 2904 3072 3156 3240 3408 3492 3576 Size: 899 766 168 84 84 168 84 84 Cuts at: 3576 3660 3996 4080 4164 4248 5603 1239 Size: 84 336 84 84 84 1355 2579 Fragments arranged by size: 2579 1355 899 766 336 168 168 84 84 84 84 84 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5068 5933 6231 5068 Size: 865 298 5780 Fragments arranged by size: 5780 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2143 5606 882 Size: 617 644 3463 2219 Fragments arranged by size: 3463 2219 644 617 BsaAI yAC'GTr Cuts at: 493 1204 4734 493 Size: 711 3530 2702 Fragments arranged by size: 3530 2702 711 BsaBI GATnn'nnATC Cuts at: 6937 6937 Size: 6943 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2849 3379 3857 4750 Size: 53 83 186 936 1316 530 478 893 Cuts at: 4750 5132 5767 275 Size: 382 635 1451 Fragments arranged by size: 1451 1316 936 893 635 530 478 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3890 5376 5774 6048 6195 6606 3890 Size: 1486 398 274 147 411 4227 Fragments arranged by size: 4227 1486 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2123 2153 2165 2195 1178 Size: 30 165 30 720 30 12 30 5926 Fragments arranged by size: 5926 720 165 30 30 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 1186 Size: 892 6051 BseYI C'CCAG_C Cuts at: 6146 6642 6146 Size: 496 6447 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3604 1163 Size: 573 1868 4502 Fragments arranged by size: 4502 1868 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4393 5154 6182 1540 Size: 275 186 2392 761 1028 2301 Fragments arranged by size: 2392 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5004 5089 6160 5004 Size: 85 1071 5787 Fragments arranged by size: 5787 1071 85 BsmI GAATG_Cn' Cuts at: 4454 4547 4454 Size: 93 6850 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4262 5004 5089 6160 1391 Size: 399 2472 742 85 1071 2174 Fragments arranged by size: 2472 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6606 6606 Size: 6943 BspHI T'CATG_A Cuts at: 2287 4832 6562 2287 Size: 2545 1730 2668 Fragments arranged by size: 2668 2545 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3626 4298 844 Size: 2782 672 3489 Fragments arranged by size: 3489 2782 672 BsrBI CCG'CTC Cuts at: 1394 1823 4830 1394 Size: 429 3007 3507 Fragments arranged by size: 3507 3007 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2222 5438 5612 65 Size: 1183 974 3216 174 1396 Fragments arranged by size: 3216 1396 1183 974 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5587 5774 1700 Size: 110 3777 187 2869 Fragments arranged by size: 3777 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6943 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6943 BssSI C'ACGA_G Cuts at: 5003 6015 5003 Size: 1012 5931 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6943 BstBI TT'CG_AA Cuts at: 5747 5747 Size: 6943 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6943 BstYI r'GATC_y Cuts at: 1972 4273 4640 5025 5042 6494 6580 6592 6938 Size: 2301 367 385 17 1452 86 12 346 Cuts at: 6938 1972 Size: 1977 Fragments arranged by size: 2301 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2578 513 Size: 552 939 574 4878 Fragments arranged by size: 4878 939 574 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6204 Fragments arranged by size: 6204 683 56 BtsI GCAGTG_nn' Cuts at: 4459 5253 5273 4459 Size: 794 20 6129 Fragments arranged by size: 6129 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6943 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6908 DraI TTT'AAA Cuts at: 4247 4247 Size: 6943 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5950 809 Size: 507 4634 1802 Fragments arranged by size: 4634 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2326 4390 Size: 54 1425 50 275 18 213 283 2064 Cuts at: 4390 5279 6908 8 Size: 889 1629 43 Fragments arranged by size: 2064 1629 1425 889 283 275 213 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4390 1537 Size: 275 2578 4090 Fragments arranged by size: 4090 2578 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2158 2469 2964 4873 1353 Size: 805 311 495 1909 3423 Fragments arranged by size: 3423 1909 805 495 311 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5530 5904 6050 387 Size: 1064 138 3941 374 146 1280 Fragments arranged by size: 3941 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2824 2904 2988 3072 3156 3240 Size: 393 506 686 80 84 84 84 84 Cuts at: 3240 3324 3408 3492 3576 3660 3832 3996 4080 Size: 84 84 84 84 84 172 164 84 Cuts at: 4080 4164 4248 5006 5603 6390 6658 1239 Size: 84 84 758 597 787 268 1524 Fragments arranged by size: 1524 787 758 686 597 506 393 268 172 164 84 84 84 84 84 84 84 84 84 84 84 84 80 EcoRI G'AATT_C Cuts at: 2051 2596 3462 3966 4252 4630 2051 Size: 545 866 504 286 378 4364 Fragments arranged by size: 4364 866 545 504 378 286 EcoRV GAT'ATC Cuts at: 2149 2149 Size: 6943 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2194 2438 2562 2194 Size: 244 124 6575 Fragments arranged by size: 6575 244 124 FspI TGC'GCA Cuts at: 5449 5449 Size: 6943 HaeII r_GCGC'y Cuts at: 1678 6090 1678 Size: 4412 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2165 2197 2827 2859 3079 Size: 32 135 32 593 32 630 32 220 Cuts at: 3079 3111 3163 3195 3499 3531 3667 3699 3835 Size: 32 52 32 304 32 136 32 136 Cuts at: 3835 3867 4003 4035 4171 4203 5584 5616 5658 Size: 32 136 32 136 32 1381 32 42 Cuts at: 5658 5690 1373 Size: 32 2626 Fragments arranged by size: 2626 1381 630 593 304 220 136 136 136 136 135 52 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2184 2361 3693 4287 4533 669 Size: 988 281 246 177 1332 594 246 3079 Fragments arranged by size: 3079 1332 988 594 281 246 246 177 HindIII A'AGCT_T Cuts at: 748 2431 2919 3255 3423 3564 3759 3927 748 Size: 1683 488 336 168 141 195 168 3764 Fragments arranged by size: 3764 1683 488 336 195 168 168 141 HpaI GTT'AAC Cuts at: 3693 4533 3693 Size: 840 6103 KpnI G_GTAC'C Cuts at: 4268 4268 Size: 6943 MfeI C'AATT_G Cuts at: 4542 4542 Size: 6943 MluI A'CGCG_T Cuts at: 6819 6819 Size: 6943 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3497 3581 3833 4001 6057 6241 1305 Size: 2192 84 252 168 2056 184 2007 Fragments arranged by size: 2192 2056 2007 252 184 168 84 MscI TGG'CCA Cuts at: 10 64 1489 1832 2045 2328 10 Size: 54 1425 343 213 283 4625 Fragments arranged by size: 4625 1425 343 283 213 54 MslI CAynn'nnrTG Cuts at: 518 1166 2247 2292 2799 2983 3487 4901 5260 Size: 648 1081 45 507 184 504 1414 359 Cuts at: 5260 5419 518 Size: 159 2042 Fragments arranged by size: 2042 1414 1081 648 507 504 359 184 159 45 MspA1I CmG'CkG Cuts at: 1606 4359 5036 6184 6429 1606 Size: 2753 677 1148 245 2120 Fragments arranged by size: 2753 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2578 513 Size: 552 939 574 4878 Fragments arranged by size: 4878 939 574 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6943 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6943 NotI GC'GGCC_GC Cuts at: 4390 4390 Size: 6943 NspI r_CATG'y Cuts at: 1132 1229 2781 2865 3117 3201 3369 3537 3873 Size: 97 1552 84 252 84 168 168 336 Cuts at: 3873 4209 4301 5846 1132 Size: 336 92 1545 2229 Fragments arranged by size: 2229 1552 1545 336 336 252 168 168 97 92 84 84 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6943 PciI A'CATG_T Cuts at: 5842 5842 Size: 6943 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6943 PmeI GTTT'AAAC Cuts at: 4247 4247 Size: 6943 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2123 2155 5012 5044 6525 6557 2123 Size: 32 2857 32 1481 32 2509 Fragments arranged by size: 2857 2509 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6943 PsiI TTA'TAA Cuts at: 4074 4513 4074 Size: 439 6504 PstI C_TGCA'G Cuts at: 830 4295 830 Size: 3465 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6943 SalI G'TCGA_C Cuts at: 1936 4285 1936 Size: 2349 4594 SbfI CC_TGCA'GG Cuts at: 4295 4295 Size: 6943 ScaI AGT'ACT Cuts at: 5191 6903 5191 Size: 1712 5231 SexAI A'CCwGG_T Cuts at: 2133 2133 Size: 6943 SfcI C'TryA_G Cuts at: 826 1045 4291 5426 6107 6298 826 Size: 219 3246 1135 681 191 1471 Fragments arranged by size: 3246 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6943 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6943 SmaI CCC'GGG Cuts at: 1872 4270 1872 Size: 2398 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2692 5047 5948 6210 820 Size: 197 926 749 2355 901 262 1553 Fragments arranged by size: 2355 1553 926 901 749 262 197 SnaBI TAC'GTA Cuts at: 493 4734 493 Size: 4241 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6943 SphI G_CATG'C Cuts at: 1132 2781 4301 1132 Size: 1649 1520 3774 Fragments arranged by size: 3774 1649 1520 SspI AAT'ATT Cuts at: 5 52 4867 5 Size: 47 4815 2081 Fragments arranged by size: 4815 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6943 StyI C'CwwG_G Cuts at: 513 1065 2004 2520 2578 4382 513 Size: 552 939 516 58 1804 3074 Fragments arranged by size: 3074 1804 939 552 516 58 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2353 2486 4970 4987 5140 5325 5770 Size: 1255 773 133 2484 17 153 185 445 Cuts at: 5770 325 Size: 1498 Fragments arranged by size: 2484 1498 1255 773 445 185 153 133 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2369 2626 5189 Size: 274 80 33 51 1443 392 257 2563 Cuts at: 5189 6901 96 Size: 1712 138 Fragments arranged by size: 2563 1712 1443 392 274 257 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2501 2533 3089 3121 3173 3205 6903 6935 2501 Size: 32 556 32 52 32 3698 32 2509 Fragments arranged by size: 3698 2509 556 52 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6943 XbaI T'CTAG_A Cuts at: 4279 4279 Size: 6943 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6943 XmaI C'CCGG_G Cuts at: 1870 4268 1870 Size: 2398 4545 XmnI GAAnn'nnTTC Cuts at: 2174 2588 5072 2174 Size: 414 2484 4045 Fragments arranged by size: 4045 2484 414 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2850 3380 3858 4751 Size: 53 83 186 936 1316 530 478 893 Cuts at: 4751 276 Size: 2468 Fragments arranged by size: 2468 1316 936 893 530 478 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI