Restriction map for
pFN21AA2033
(Linear) (Six-base) MAPSORT of: KIAA2033.seq Check: 4009 from: 1 to: 2238
KIAA2033.orf
With 182 enzymes: SgfI *
August 7, 2008 14:13 ..
AatII G_ACGT'C
Cuts at: 0 847 1377 1855 2238
Size: 847 530 478 383
Fragments arranged by size:
847 530 478 383
AccI GT'mk_AC
Cuts at: 0 867 1203 1371 1623 1707 1875 2211 2238
Size: 867 336 168 252 84 168 336 27
Fragments arranged by size:
867 336 336 252 168 168 84 27
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 819 983 1319 1827 2238
Size: 819 164 336 508 411
Fragments arranged by size:
819 508 411 336 164
AflII C'TTAA_G
Cuts at: 0 687 2238
Size: 687 1551
AhdI GACnn_n'nnGTC
Cuts at: 0 836 1088 1172 1508 1844 2012 2180 2238
Size: 836 252 84 336 336 168 168 58
Fragments arranged by size:
836 336 336 252 168 168 84 58
AleI CACnn'nnGTG
Cuts at: 0 794 2238
Size: 794 1444
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 118 150 2238
Size: 118 32 2088
Fragments arranged by size:
2088 118 32
ApoI r'AATT_y
Cuts at: 0 46 292 536 591 1269 1289 1457 1793
Size: 46 246 244 55 678 20 168 336
Cuts at: 1793 1961 2238
Size: 168 277
Fragments arranged by size:
678 336 277 246 244 168 168 55
46 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 1307 2238
Size: 1307 931
BclI T'GATC_A
Cuts at: 0 378 2238
Size: 378 1860
BmgBI CAC'GTC
Cuts at: 0 105 2238
Size: 105 2133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 899 1067 1151 1235 1403 1487 1571
Size: 133 766 168 84 84 168 84 84
Cuts at: 1571 1655 1991 2075 2159 2238
Size: 84 336 84 84 79
Fragments arranged by size:
766 336 168 168 133 84 84 84
84 84 84 84 79
BsaI GGTCTCn'nnnn_
Cuts at: 0 138 2238
Size: 138 2100
BsaHI Gr'CG_yC
Cuts at: 0 844 1374 1852 2238
Size: 844 530 478 386
Fragments arranged by size:
844 530 478 386
BsaWI w'CCGG_w
Cuts at: 0 1885 2238
Size: 1885 353
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 118 148 160 190 2238
Size: 118 30 12 30 2048
Fragments arranged by size:
2048 118 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 2238
Size: 73 2165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1599 2238
Size: 1599 639
BspHI T'CATG_A
Cuts at: 0 282 2238
Size: 282 1956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1621 2238
Size: 1621 617
BsrDI GCAATG_nn'
Cuts at: 0 217 2238
Size: 217 2021
BtgI C'CryG_G
Cuts at: 0 573 2238
Size: 573 1665
EaeI y'GGCC_r
Cuts at: 0 38 321 2238
Size: 38 283 1917
Fragments arranged by size:
1917 283 38
EarI CTCTTCn'nnn_
Cuts at: 0 153 464 959 2238
Size: 153 311 495 1279
Fragments arranged by size:
1279 495 311 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 819 899 983 1067 1151 1235 1319
Size: 133 686 80 84 84 84 84 84
Cuts at: 1319 1403 1487 1571 1655 1827 1991 2075 2159
Size: 84 84 84 84 172 164 84 84
Cuts at: 2159 2238
Size: 79
Fragments arranged by size:
686 172 164 133 84 84 84 84
84 84 84 84 84 84 84 80
79
EcoRI G'AATT_C
Cuts at: 0 46 591 1457 1961 2238
Size: 46 545 866 504 277
Fragments arranged by size:
866 545 504 277 46
EcoRV GAT'ATC
Cuts at: 0 144 2238
Size: 144 2094
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 189 433 557 2238
Size: 189 244 124 1681
Fragments arranged by size:
1681 244 189 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 822 854 1074 1106 1158 1190
Size: 160 32 630 32 220 32 52 32
Cuts at: 1190 1494 1526 1662 1694 1830 1862 1998 2030
Size: 304 32 136 32 136 32 136 32
Cuts at: 2030 2166 2198 2238
Size: 136 32 40
Fragments arranged by size:
630 304 220 160 136 136 136 136
52 40 32 32 32 32 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 179 356 1688 2238
Size: 179 177 1332 550
Fragments arranged by size:
1332 550 179 177
HindIII A'AGCT_T
Cuts at: 0 426 914 1250 1418 1559 1754 1922 2238
Size: 426 488 336 168 141 195 168 316
Fragments arranged by size:
488 426 336 316 195 168 168 141
HpaI GTT'AAC
Cuts at: 0 1688 2238
Size: 1688 550
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1492 1576 1828 1996 2238
Size: 1492 84 252 168 242
Fragments arranged by size:
1492 252 242 168 84
MscI TGG'CCA
Cuts at: 0 40 323 2238
Size: 40 283 1915
Fragments arranged by size:
1915 283 40
MslI CAynn'nnrTG
Cuts at: 0 242 287 794 978 1482 2238
Size: 242 45 507 184 504 756
Fragments arranged by size:
756 507 504 242 184 45
NcoI C'CATG_G
Cuts at: 0 573 2238
Size: 573 1665
NspI r_CATG'y
Cuts at: 0 776 860 1112 1196 1364 1532 1868 2204
Size: 776 84 252 84 168 168 336 336
Cuts at: 2204 2238
Size: 34
Fragments arranged by size:
776 336 336 252 168 168 84 84
34
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 118 150 2238
Size: 118 32 2088
Fragments arranged by size:
2088 118 32
PsiI TTA'TAA
Cuts at: 0 2069 2238
Size: 2069 169
SexAI A'CCwGG_T
Cuts at: 0 128 2238
Size: 128 2110
SmlI C'TyrA_G
Cuts at: 0 687 2238
Size: 687 1551
SphI G_CATG'C
Cuts at: 0 776 2238
Size: 776 1462
StyI C'CwwG_G
Cuts at: 0 515 573 2238
Size: 515 58 1665
Fragments arranged by size:
1665 515 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 348 481 2238
Size: 348 133 1757
Fragments arranged by size:
1757 348 133
TatI w'GTAC_w
Cuts at: 0 364 621 2238
Size: 364 257 1617
Fragments arranged by size:
1617 364 257
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 496 528 1084 1116 1168 1200 2238
Size: 496 32 556 32 52 32 1038
Fragments arranged by size:
1038 556 496 52 32 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 169 583 2238
Size: 169 414 1655
Fragments arranged by size:
1655 414 169
ZraI GAC'GTC
Cuts at: 0 845 1375 1853 2238
Size: 845 530 478 385
Fragments arranged by size:
845 530 478 385
Enzymes that do cut:
AatII AccI AcuI AflII AhdI AleI AloI ApoI
BbsI BclI BmgBI BpmI BsaI BsaHI BsaWI BsaXI
BseRI BsgI BspHI BspMI BsrDI BtgI EaeI EarI
Eco57MI EcoRI EcoRV FalI Hin4I HincII HindIII HpaI
MmeI MscI MslI NcoI NspI PpiI PsiI SexAI
SmlI SphI StyI TaqII TatI TstI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflIII AgeI AlfI AlwNI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BseYI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoO109I FseI FspI FspAI HaeII KasI
KpnI MfeI MluI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI Tth111I
XbaI XcmI XhoI XmaI