(Circular) (Six-base) MAPSORT of: pFN21AB0999.seq Check: 5429 from: 1 to: 12055 pFN21AB0999 12055 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2262 4772 6876 7254 7780 8080 8352 2262 Size: 2510 2104 378 526 300 272 5965 Fragments arranged by size: 5965 2510 2104 526 378 300 272 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2115 5739 6788 8666 Size: 53 83 186 936 579 3624 1049 1878 Cuts at: 8666 9865 278 Size: 1199 2468 Fragments arranged by size: 3624 2468 1878 1199 1049 936 579 186 83 53 AccI GT'mk_AC Cuts at: 1937 6788 7812 9398 1937 Size: 4851 1024 1586 4594 Fragments arranged by size: 4851 4594 1586 1024 Acc65I G'GTAC_C Cuts at: 9376 9376 Size: 12055 AclI AA'CG_TT Cuts at: 1563 10182 10555 1563 Size: 8619 373 3063 Fragments arranged by size: 8619 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2421 2603 2739 4037 4188 4425 4913 4922 Size: 789 182 136 1298 151 237 488 9 Cuts at: 4922 5503 6263 6269 6572 7315 8827 10118 11502 Size: 581 760 6 303 743 1512 1291 1384 Cuts at: 11502 11770 1632 Size: 268 1917 Fragments arranged by size: 1917 1512 1384 1298 1291 789 760 743 581 488 303 268 237 182 151 136 9 6 AfeI AGC'GCT Cuts at: 2906 2906 Size: 12055 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 11858 AflIII A'CryG_T Cuts at: 1203 3294 10954 11931 1203 Size: 2091 7660 977 1327 Fragments arranged by size: 7660 2091 1327 977 AgeI A'CCGG_T Cuts at: 2443 10886 2443 Size: 8443 3612 AhdI GACnn_n'nnGTC Cuts at: 10784 10784 Size: 12055 AleI CACnn'nnGTG Cuts at: 1166 2035 5675 7487 7694 8445 1166 Size: 869 3640 1812 207 751 4776 Fragments arranged by size: 4776 3640 1812 869 751 207 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2699 5412 5693 1184 Size: 1515 2713 281 7546 Fragments arranged by size: 7546 2713 1515 281 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2879 2911 8124 8156 2879 Size: 32 5213 32 6778 Fragments arranged by size: 6778 5213 32 32 AlwNI CAG_nnn'CTG Cuts at: 3395 4226 5152 6232 6968 8134 9798 11370 3395 Size: 831 926 1080 736 1166 1664 1572 4080 Fragments arranged by size: 4080 1664 1572 1166 1080 926 831 736 ApaI G_GGCC'C Cuts at: 2754 4305 5468 6239 9273 2754 Size: 1551 1163 771 3034 5536 Fragments arranged by size: 5536 3034 1551 1163 771 ApaLI G'TGCA_C Cuts at: 4600 6488 9101 10112 11268 4600 Size: 1888 2613 1011 1156 5387 Fragments arranged by size: 5387 2613 1888 1156 1011 ApoI r'AATT_y Cuts at: 1493 9364 9590 9742 11851 1493 Size: 7871 226 152 2109 1697 Fragments arranged by size: 7871 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 10609 160 Size: 872 9577 1606 Fragments arranged by size: 9577 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12055 AvaI C'yCGr_G Cuts at: 1870 1943 2225 2282 2627 4729 4857 4959 5061 Size: 73 282 57 345 2102 128 102 102 Cuts at: 5061 5381 5782 6338 6516 6580 6830 7212 7857 Size: 320 401 556 178 64 250 382 645 Cuts at: 7857 7881 9058 9380 1870 Size: 24 1177 322 4545 Fragments arranged by size: 4545 2102 1177 645 556 401 382 345 322 320 282 250 178 128 102 102 73 64 57 24 BamHI G'GATC_C Cuts at: 2012 6102 9385 9752 2012 Size: 4090 3283 367 4315 Fragments arranged by size: 4315 4090 3283 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2491 3468 4195 4325 4733 Size: 325 217 627 704 977 727 130 408 Cuts at: 4733 5083 5396 5512 5584 5677 5750 6389 6602 Size: 350 313 116 72 93 73 639 213 Cuts at: 6602 6670 7274 7796 8065 8475 8600 9376 10831 Size: 68 604 522 269 410 125 776 1455 Cuts at: 10831 618 Size: 1842 Fragments arranged by size: 1842 1455 977 776 727 704 639 627 604 522 410 408 350 325 313 269 217 213 130 125 116 93 73 72 68 BanII G_rGCy'C Cuts at: 1391 2055 2121 2250 2754 3932 4305 4392 4420 Size: 664 66 129 504 1178 373 87 28 Cuts at: 4420 4587 4730 4956 5081 5468 5633 6239 6377 Size: 167 143 226 125 387 165 606 138 Cuts at: 6377 6590 6647 6723 7704 7833 7941 7964 7971 Size: 213 57 76 981 129 108 23 7 Cuts at: 7971 8193 8210 8238 8259 8790 8804 9273 9309 Size: 222 17 28 21 531 14 469 36 Cuts at: 9309 9374 1391 Size: 65 4072 Fragments arranged by size: 4072 1178 981 664 606 531 504 469 387 373 226 222 213 167 165 143 138 129 129 125 108 87 76 66 65 57 36 28 28 23 21 17 14 7 BbeI G_GCGC'C Cuts at: 3472 4329 4737 6606 8069 3472 Size: 857 408 1869 1463 7458 Fragments arranged by size: 7458 1869 1463 857 408 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2409 3392 3639 3656 3739 928 Size: 971 510 983 247 17 83 9244 Fragments arranged by size: 9244 983 971 510 247 83 17 BbvCI CC'TCA_GC Cuts at: 4548 4653 5040 5245 5998 7157 4548 Size: 105 387 205 753 1159 9446 Fragments arranged by size: 9446 1159 753 387 205 105 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4067 4101 4425 4459 4739 4773 6854 Size: 34 2130 34 324 34 280 34 2081 Cuts at: 6854 6888 10246 10280 1903 Size: 34 3358 34 3678 Fragments arranged by size: 3678 3358 2130 2081 324 280 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 9947 11157 9947 Size: 1210 10845 BclI T'GATC_A Cuts at: 1548 5214 9204 1548 Size: 3666 3990 4399 Fragments arranged by size: 4399 3990 3666 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2191 2430 2500 Size: 107 122 71 631 605 519 239 70 Cuts at: 2500 3075 3815 4655 4670 5064 5600 5982 7791 Size: 575 740 840 15 394 536 382 1809 Cuts at: 7791 7876 10666 136 Size: 85 2790 1525 Fragments arranged by size: 2790 1809 1525 840 740 631 605 575 536 519 394 382 239 122 107 85 71 70 15 BglII A'GATC_T Cuts at: 2571 3996 8964 12050 2571 Size: 1425 4968 3086 2576 Fragments arranged by size: 4968 3086 2576 1425 BlpI GC'TnA_GC Cuts at: 8492 9472 8492 Size: 980 11075 Bme1580I G_kGCm'C Cuts at: 1790 2754 4200 4305 4604 5202 5468 5589 5682 Size: 964 1446 105 299 598 266 121 93 Cuts at: 5682 5753 6176 6239 6392 6492 6523 6673 6831 Size: 71 423 63 153 100 31 150 158 Cuts at: 6831 7263 8605 8736 9105 9273 10116 11272 1790 Size: 432 1342 131 369 168 843 1156 2573 Fragments arranged by size: 2573 1446 1342 1156 964 843 598 432 423 369 299 266 168 158 153 150 131 121 105 100 93 71 63 31 BmgBI CAC'GTC Cuts at: 1318 2037 5431 5830 5857 7401 7476 1318 Size: 719 3394 399 27 1544 75 5897 Fragments arranged by size: 5897 3394 1544 719 399 75 27 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2078 4505 5884 Size: 416 53 83 399 24 661 2427 1379 Cuts at: 5884 7647 7799 8208 8562 9786 10744 442 Size: 1763 152 409 354 1224 958 1753 Fragments arranged by size: 2427 1763 1753 1379 1224 958 661 416 409 399 354 152 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 12055 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2287 3835 7960 8881 9358 2287 Size: 1548 4125 921 477 4984 Fragments arranged by size: 4984 4125 1548 921 477 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 2504 2624 3179 3398 4738 7085 7528 Size: 905 360 120 555 219 1340 2347 443 Cuts at: 7528 7625 7955 8294 8372 8631 8796 10715 1239 Size: 97 330 339 78 259 165 1919 2579 Fragments arranged by size: 2579 2347 1919 1340 905 555 443 360 339 330 259 219 165 120 97 78 Bpu10I CC'TnA_GC Cuts at: 2478 4548 4653 5040 5245 5920 5998 6621 7157 Size: 2070 105 387 205 675 78 623 536 Cuts at: 7157 2478 Size: 7376 Fragments arranged by size: 7376 2070 675 623 536 387 205 105 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3626 4661 6350 6383 10180 11045 11343 3626 Size: 1035 1689 33 3797 865 298 4338 Fragments arranged by size: 4338 3797 1689 1035 865 298 33 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3311 3887 4487 6020 7416 7504 7642 Size: 617 1812 576 600 1533 1396 88 138 Cuts at: 7642 8649 9022 10718 882 Size: 1007 373 1696 2219 Fragments arranged by size: 2219 1812 1696 1533 1396 1007 617 600 576 373 138 88 BsaAI yAC'GTr Cuts at: 493 1204 6118 9846 493 Size: 711 4914 3728 2702 Fragments arranged by size: 4914 3728 2702 711 BsaBI GATnn'nnATC Cuts at: 12049 12049 Size: 12055 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2112 3469 4124 4326 Size: 53 83 186 936 579 1357 655 202 Cuts at: 4326 4734 5034 5603 5736 6513 6603 6785 6791 Size: 408 300 569 133 777 90 182 6 Cuts at: 6791 8066 8663 9131 9862 10244 10879 275 Size: 1275 597 468 731 382 635 1451 Fragments arranged by size: 1451 1357 1275 936 777 731 655 635 597 579 569 468 408 382 300 202 186 182 133 90 83 53 6 BsaWI w'CCGG_w Cuts at: 2443 2706 4363 4412 5569 8710 8922 8987 10488 Size: 263 1657 49 1157 3141 212 65 1501 Cuts at: 10488 10886 11160 11307 11718 2443 Size: 398 274 147 411 2780 Fragments arranged by size: 3141 2780 1657 1501 1157 411 398 274 263 212 147 65 49 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2138 2168 2768 2798 2879 Size: 30 165 30 735 30 600 30 81 Cuts at: 2879 2909 4736 4766 5528 5558 5693 5723 6434 Size: 30 1827 30 762 30 135 30 711 Cuts at: 6434 6464 6917 6947 7334 7364 7501 7531 8124 Size: 30 453 30 387 30 137 30 593 Cuts at: 8124 8154 9237 9267 1178 Size: 30 1083 30 3966 Fragments arranged by size: 3966 1827 1083 762 735 711 600 593 453 387 165 137 135 81 30 30 30 30 30 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2528 2621 2624 2875 3572 3614 3617 3629 Size: 1342 93 3 251 697 42 3 12 Cuts at: 3629 3797 4094 4127 4160 4163 4175 4254 4449 Size: 168 297 33 33 3 12 79 195 Cuts at: 4449 4493 4502 4505 4532 4541 4565 4595 4631 Size: 44 9 3 27 9 24 30 36 Cuts at: 4631 4826 5347 5986 6335 6557 7729 7936 9085 Size: 195 521 639 349 222 1172 207 1149 Cuts at: 9085 9317 1186 Size: 232 3924 Fragments arranged by size: 3924 1342 1172 1149 697 639 521 349 297 251 232 222 207 195 195 168 93 79 44 42 36 33 33 30 27 24 12 12 9 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 2187 2287 2456 3798 4219 4312 4829 4993 5125 Size: 100 169 1342 421 93 517 164 132 Cuts at: 5125 5372 5561 5666 6625 7140 7279 7323 7703 Size: 247 189 105 959 515 139 44 380 Cuts at: 7703 7918 7952 8436 8789 9029 11258 11754 2187 Size: 215 34 484 353 240 2229 496 2488 Fragments arranged by size: 2488 2229 1342 959 517 515 496 484 421 380 353 247 240 215 189 169 164 139 132 105 100 93 44 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2108 2705 2709 4437 4791 1163 Size: 573 372 597 4 1728 354 8427 Fragments arranged by size: 8427 1728 597 573 372 354 4 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2363 3099 3898 4665 5803 5943 Size: 275 186 362 736 799 767 1138 140 Cuts at: 5943 6362 6472 8351 9505 10266 11294 1540 Size: 419 110 1879 1154 761 1028 2301 Fragments arranged by size: 2301 1879 1154 1138 1028 799 767 761 736 419 362 275 186 140 110 BsiHKAI G_wGCw'C Cuts at: 2250 3114 4604 4730 5897 6492 6837 6867 7257 Size: 864 1490 126 1167 595 345 30 390 Cuts at: 7257 7497 8210 8259 9105 10116 10201 11272 2250 Size: 240 713 49 846 1011 85 1071 3033 Fragments arranged by size: 3033 1490 1167 1071 1011 864 846 713 595 390 345 240 126 85 49 30 BsiWI C'GTAC_G Cuts at: 6445 6445 Size: 12055 BsmI GAATG_Cn' Cuts at: 4088 4214 5661 9566 9659 4088 Size: 126 1447 3905 93 6484 Fragments arranged by size: 6484 3905 1447 126 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3448 5930 3448 Size: 2482 9573 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2121 2250 2754 3114 3932 4200 Size: 399 265 66 129 504 360 818 268 Cuts at: 4200 4305 4392 4420 4587 4604 4730 4956 5081 Size: 105 87 28 167 17 126 226 125 Cuts at: 5081 5202 5468 5589 5633 5682 5753 5897 6176 Size: 121 266 121 44 49 71 144 279 Cuts at: 6176 6239 6377 6392 6492 6523 6590 6647 6673 Size: 63 138 15 100 31 67 57 26 Cuts at: 6673 6723 6831 6837 6867 7257 7263 7497 7704 Size: 50 108 6 30 390 6 234 207 Cuts at: 7704 7833 7941 7964 7971 8193 8210 8238 8259 Size: 129 108 23 7 222 17 28 21 Cuts at: 8259 8605 8736 8790 8804 9105 9273 9309 9374 Size: 346 131 54 14 301 168 36 65 Cuts at: 9374 10116 10201 11272 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 818 742 504 399 390 360 346 301 279 268 266 265 234 226 222 207 168 167 144 138 131 129 129 126 125 121 121 108 108 105 100 87 85 71 67 66 65 63 57 54 50 49 44 36 31 30 28 28 26 23 21 17 17 15 14 7 6 6 BspEI T'CCGG_A Cuts at: 11718 11718 Size: 12055 BspHI T'CATG_A Cuts at: 2803 3288 9944 11674 2803 Size: 485 6656 1730 3184 Fragments arranged by size: 6656 3184 1730 485 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2262 2286 4772 5025 6147 6876 6987 7254 Size: 1418 24 2486 253 1122 729 111 267 Cuts at: 7254 7780 8080 8352 9164 9410 844 Size: 526 300 272 812 246 3489 Fragments arranged by size: 3489 2486 1418 1122 812 729 526 300 272 267 253 246 111 24 BsrBI CCG'CTC Cuts at: 1394 1823 3756 4137 4704 4913 6297 6364 6397 Size: 429 1933 381 567 209 1384 67 33 Cuts at: 6397 7414 7438 7456 7876 7926 9136 9942 1394 Size: 1017 24 18 420 50 1210 806 3507 Fragments arranged by size: 3507 1933 1384 1210 1017 806 567 429 420 381 209 67 50 33 24 18 BsrDI GCAATG_nn' Cuts at: 65 1248 6591 10550 10724 65 Size: 1183 5343 3959 174 1396 Fragments arranged by size: 5343 3959 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2443 4063 4624 4809 6253 6410 6558 Size: 110 633 1620 561 185 1444 157 148 Cuts at: 6558 6680 7071 7892 8062 9078 10699 10886 1700 Size: 122 391 821 170 1016 1621 187 2869 Fragments arranged by size: 2869 1621 1620 1444 1016 821 633 561 391 187 185 170 157 148 122 110 BsrGI T'GTAC_A Cuts at: 96 2772 3270 5742 96 Size: 2676 498 2472 6409 Fragments arranged by size: 6409 2676 2472 498 BssHII G'CGCG_C Cuts at: 1925 6596 1925 Size: 4671 7384 BssSI C'ACGA_G Cuts at: 3113 6693 6795 7256 10115 11127 3113 Size: 3580 102 461 2859 1012 4041 Fragments arranged by size: 4041 3580 2859 1012 461 102 BstAPI GCAn_nnn'nTGC Cuts at: 794 3538 5017 6677 6968 7111 794 Size: 2744 1479 1660 291 143 5738 Fragments arranged by size: 5738 2744 1660 1479 291 143 BstBI TT'CG_AA Cuts at: 10859 10859 Size: 12055 BstEII G'GTnAC_C Cuts at: 1184 4936 5270 8153 8411 8425 1184 Size: 3752 334 2883 258 14 4814 Fragments arranged by size: 4814 3752 2883 334 258 14 BstXI CCAn_nnnn'nTGG Cuts at: 5509 7135 7291 7489 8248 8797 5509 Size: 1626 156 198 759 549 8767 Fragments arranged by size: 8767 1626 759 549 198 156 BstYI r'GATC_y Cuts at: 1972 2012 2396 2571 3021 3996 6102 6309 8964 Size: 40 384 175 450 975 2106 207 2655 Cuts at: 8964 9385 9752 10137 10154 11606 11692 11704 12050 Size: 421 367 385 17 1452 86 12 346 Cuts at: 12050 1972 Size: 1977 Fragments arranged by size: 2655 2106 1977 1452 975 450 421 385 384 367 346 207 175 86 40 17 12 Bsu36I CC'TnA_GG Cuts at: 3664 7721 3664 Size: 4057 7998 BtgI C'CryG_G Cuts at: 513 1065 2379 2498 2556 3472 3822 4098 4542 Size: 552 1314 119 58 916 350 276 444 Cuts at: 4542 4659 5475 5493 5549 5704 5719 5797 6082 Size: 117 816 18 56 155 15 78 285 Cuts at: 6082 6276 6356 6635 6664 6943 7039 7134 7290 Size: 194 80 279 29 279 96 95 156 Cuts at: 7290 7713 8158 8596 8611 8871 9258 513 Size: 423 445 438 15 260 387 3310 Fragments arranged by size: 3310 1314 916 816 552 445 444 438 423 387 350 285 279 279 276 260 194 156 155 119 117 96 95 80 78 58 56 29 18 15 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2843 3898 4428 7993 487 Size: 683 56 1617 1055 530 3565 4549 Fragments arranged by size: 4549 3565 1617 1055 683 530 56 BtsI GCAGTG_nn' Cuts at: 3085 3953 4219 4566 4774 5276 5298 7780 9314 Size: 868 266 347 208 502 22 2482 1534 Cuts at: 9314 9571 10365 10385 3085 Size: 257 794 20 4755 Fragments arranged by size: 4755 2482 1534 868 794 502 347 266 257 208 22 20 ClaI AT'CG_AT Cuts at: 1554 3132 1554 Size: 1578 10477 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 12020 DraI TTT'AAA Cuts at: 9359 9359 Size: 12055 DraIII CAC_nnn'GTG Cuts at: 3118 8570 3118 Size: 5452 6603 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3284 11062 809 Size: 507 1968 7778 1802 Fragments arranged by size: 7778 1968 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2258 2376 3096 Size: 54 1425 50 275 18 428 118 720 Cuts at: 3096 3180 3252 3895 3909 4071 4626 4662 5800 Size: 84 72 643 14 162 555 36 1138 Cuts at: 5800 5940 6273 6359 6469 6507 6560 6682 7125 Size: 140 333 86 110 38 53 122 443 Cuts at: 7125 7868 7894 7996 8348 9080 9502 10391 12020 Size: 743 26 102 352 732 422 889 1629 Cuts at: 12020 8 Size: 43 Fragments arranged by size: 1629 1425 1138 889 743 732 720 643 555 443 428 422 352 333 275 162 140 122 118 110 102 86 84 72 54 53 50 43 38 36 26 18 14 EagI C'GGCC_G Cuts at: 1537 1812 3096 3895 4662 5800 5940 6359 6469 Size: 275 1284 799 767 1138 140 419 110 Cuts at: 6469 8348 9502 1537 Size: 1879 1154 4090 Fragments arranged by size: 4090 1879 1284 1154 1138 799 767 419 275 140 110 EarI CTCTTCn'nnn_ Cuts at: 1353 3971 6548 8247 9985 1353 Size: 2618 2577 1699 1738 3423 Fragments arranged by size: 3423 2618 2577 1738 1699 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2209 3605 5658 6171 8546 8857 Size: 1064 138 620 1396 2053 513 2375 311 Cuts at: 8857 10642 11016 11162 387 Size: 1785 374 146 1280 Fragments arranged by size: 2375 2053 1785 1396 1280 1064 620 513 374 311 146 138 EcoICRI GAG'CTC Cuts at: 2248 4728 8208 8257 2248 Size: 2480 3480 49 6046 Fragments arranged by size: 6046 3480 2480 49 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2421 2504 2603 2624 2739 3179 Size: 393 512 277 83 99 21 115 440 Cuts at: 3179 3398 4037 4188 4425 4738 4913 4922 5503 Size: 219 639 151 237 313 175 9 581 Cuts at: 5503 6263 6269 6572 7085 7315 7528 7625 7955 Size: 760 6 303 513 230 213 97 330 Cuts at: 7955 8294 8372 8631 8796 8827 10118 10715 11502 Size: 339 78 259 165 31 1291 597 787 Cuts at: 11502 11770 1239 Size: 268 1524 Fragments arranged by size: 1524 1291 787 760 639 597 581 513 512 440 393 339 330 313 303 277 268 259 237 230 219 213 175 165 151 115 99 97 83 78 31 21 9 6 EcoNI CCTnn'n_nnAGG Cuts at: 2411 3641 5744 5819 6209 6737 2411 Size: 1230 2103 75 390 528 7729 Fragments arranged by size: 7729 2103 1230 528 390 75 EcoO109I rG'GnC_Cy Cuts at: 2199 2750 2751 5161 5455 5465 5924 6202 6229 Size: 551 1 2410 294 10 459 278 27 Cuts at: 6229 6236 6265 7162 7208 7852 8303 9162 9270 Size: 7 29 897 46 644 451 859 108 Cuts at: 9270 2199 Size: 4984 Fragments arranged by size: 4984 2410 897 859 644 551 459 451 294 278 108 46 29 27 10 7 1 EcoRI G'AATT_C Cuts at: 9364 9742 9364 Size: 378 11677 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2863 3337 2863 Size: 474 11581 FseI GG_CCGG'CC Cuts at: 4628 4628 Size: 12055 FspI TGC'GCA Cuts at: 10561 10561 Size: 12055 HaeII r_GCGC'y Cuts at: 1678 2326 2908 3472 3810 4064 4329 4508 4676 Size: 648 582 564 338 254 265 179 168 Cuts at: 4676 4737 5694 6606 7064 8069 11202 1678 Size: 61 957 912 458 1005 3133 2531 Fragments arranged by size: 3133 2531 1005 957 912 648 582 564 458 338 265 254 179 168 61 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2138 2170 2851 2883 3125 Size: 32 135 32 566 32 681 32 242 Cuts at: 3125 3157 3647 3679 4736 4768 5528 5560 6434 Size: 32 490 32 1057 32 760 32 874 Cuts at: 6434 6466 6917 6949 7206 7238 9149 9181 10696 Size: 32 451 32 257 32 1911 32 1515 Cuts at: 10696 10728 10770 10802 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1911 1515 1057 874 760 681 566 490 451 257 242 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4231 6789 9399 9645 669 Size: 988 281 2293 2558 2610 246 3079 Fragments arranged by size: 3079 2610 2558 2293 988 281 246 HindIII A'AGCT_T Cuts at: 748 8312 748 Size: 7564 4491 HpaI GTT'AAC Cuts at: 9645 9645 Size: 12055 KasI G'GCGC_C Cuts at: 3468 4325 4733 6602 8065 3468 Size: 857 408 1869 1463 7458 Fragments arranged by size: 7458 1869 1463 857 408 KpnI G_GTAC'C Cuts at: 9380 9380 Size: 12055 MfeI C'AATT_G Cuts at: 9654 9654 Size: 12055 MluI A'CGCG_T Cuts at: 11931 11931 Size: 12055 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2811 5361 7397 9369 11169 11353 1305 Size: 1506 2550 2036 1972 1800 184 2007 Fragments arranged by size: 2550 2036 2007 1972 1800 1506 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2378 6275 7998 10 Size: 54 1425 343 546 3897 1723 4067 Fragments arranged by size: 4067 3897 1723 1425 546 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2035 3236 3266 3293 5675 5882 7133 Size: 648 869 1201 30 27 2382 207 1251 Cuts at: 7133 7289 7487 7593 7673 7694 8445 10013 10372 Size: 156 198 106 80 21 751 1568 359 Cuts at: 10372 10531 518 Size: 159 2042 Fragments arranged by size: 2382 2042 1568 1251 1201 869 751 648 359 207 198 159 156 106 80 30 27 21 MspA1I CmG'CkG Cuts at: 1606 2191 2602 2770 3073 3178 3474 4544 4652 Size: 585 411 168 303 105 296 1070 108 Cuts at: 4652 4900 5523 5542 5706 5732 5799 6084 6358 Size: 248 623 19 164 26 67 285 274 Cuts at: 6358 6496 6637 6945 6968 7041 7533 7542 7551 Size: 138 141 308 23 73 492 9 9 Cuts at: 7551 7694 8353 8383 8419 8538 8758 9260 9267 Size: 143 659 30 36 119 220 502 7 Cuts at: 9267 9471 10148 11296 11541 1606 Size: 204 677 1148 245 2120 Fragments arranged by size: 2120 1148 1070 677 659 623 585 502 492 411 308 303 296 285 274 248 245 220 204 168 164 143 141 138 119 108 105 73 67 36 30 26 23 19 9 9 7 NaeI GCC'GGC Cuts at: 4065 4626 6255 6560 6682 8064 4065 Size: 561 1629 305 122 1382 8056 Fragments arranged by size: 8056 1629 1382 561 305 122 NarI GG'CG_CC Cuts at: 3469 4326 4734 6603 8066 3469 Size: 857 408 1869 1463 7458 Fragments arranged by size: 7458 1869 1463 857 408 NcoI C'CATG_G Cuts at: 513 1065 2556 3822 5475 5493 7134 7290 8596 Size: 552 1491 1266 1653 18 1641 156 1306 Cuts at: 8596 8871 513 Size: 275 3697 Fragments arranged by size: 3697 1653 1641 1491 1306 1266 552 275 156 18 NdeI CA'TA_TG Cuts at: 387 387 Size: 12055 NgoMIV G'CCGG_C Cuts at: 4063 4624 6253 6558 6680 8062 4063 Size: 561 1629 305 122 1382 8056 Fragments arranged by size: 8056 1629 1382 561 305 122 NheI G'CTAG_C Cuts at: 1052 1052 Size: 12055 NotI GC'GGCC_GC Cuts at: 6359 8348 9502 6359 Size: 1989 1154 8912 Fragments arranged by size: 8912 1989 1154 NspI r_CATG'y Cuts at: 1132 1229 3055 3298 4181 5234 5450 5881 9413 Size: 97 1826 243 883 1053 216 431 3532 Cuts at: 9413 10958 1132 Size: 1545 2229 Fragments arranged by size: 3532 2229 1826 1545 1053 883 431 243 216 97 PasI CC'CwG_GG Cuts at: 1348 4321 5284 6232 8399 8528 1348 Size: 2973 963 948 2167 129 4875 Fragments arranged by size: 4875 2973 2167 963 948 129 PciI A'CATG_T Cuts at: 3294 10954 3294 Size: 7660 4395 PflMI CCAn_nnn'nTGG Cuts at: 1108 2530 2920 8134 8422 1108 Size: 1422 390 5214 288 4741 Fragments arranged by size: 5214 4741 1422 390 288 PfoI T'CCnGG_A Cuts at: 2121 3324 3375 3885 8127 8547 8738 2121 Size: 1203 51 510 4242 420 191 5438 Fragments arranged by size: 5438 4242 1203 510 420 191 51 PmeI GTTT'AAAC Cuts at: 9359 9359 Size: 12055 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2291 2323 2879 2911 2996 3028 8124 8156 10124 Size: 32 556 32 85 32 5096 32 1968 Cuts at: 10124 10156 11637 11669 2291 Size: 32 1481 32 2677 Fragments arranged by size: 5096 2677 1968 1481 556 85 32 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5161 5924 6202 7208 5161 Size: 763 278 1006 10008 Fragments arranged by size: 10008 1006 763 278 PshAI GACnn'nnGTC Cuts at: 1613 8666 1613 Size: 7053 5002 PsiI TTA'TAA Cuts at: 9625 9625 Size: 12055 PspOMI G'GGCC_C Cuts at: 2750 4301 5464 6235 9269 2750 Size: 1551 1163 771 3034 5536 Fragments arranged by size: 5536 3034 1551 1163 771 PstI C_TGCA'G Cuts at: 830 3045 4769 5022 6738 9219 9407 830 Size: 2215 1724 253 1716 2481 188 3478 Fragments arranged by size: 3478 2481 2215 1724 1716 253 188 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12055 PvuII CAG'CTG Cuts at: 2602 3178 4900 5542 5732 8538 2602 Size: 576 1722 642 190 2806 6119 Fragments arranged by size: 6119 2806 1722 642 576 190 SacI G_AGCT'C Cuts at: 2250 4730 8210 8259 2250 Size: 2480 3480 49 6046 Fragments arranged by size: 6046 3480 2480 49 SacII CC_GC'GG Cuts at: 3475 4545 5707 5800 6085 6359 6638 6946 7042 Size: 1070 1162 93 285 274 279 308 96 Cuts at: 7042 9261 3475 Size: 2219 6269 Fragments arranged by size: 6269 2219 1162 1070 308 285 279 274 96 93 SalI G'TCGA_C Cuts at: 1936 6787 9397 1936 Size: 4851 2610 4594 Fragments arranged by size: 4851 4594 2610 SanDI GG'GwC_CC Cuts at: 5161 7208 5161 Size: 2047 10008 SapI GCTCTTCn'nnn_ Cuts at: 6548 8247 6548 Size: 1699 10356 SbfI CC_TGCA'GG Cuts at: 6738 9407 6738 Size: 2669 9386 ScaI AGT'ACT Cuts at: 2999 10303 12015 2999 Size: 7304 1712 3039 Fragments arranged by size: 7304 3039 1712 SexAI A'CCwGG_T Cuts at: 5266 7513 5266 Size: 2247 9808 SfcI C'TryA_G Cuts at: 826 1045 3041 4765 5018 6734 8170 9215 9403 Size: 219 1996 1724 253 1716 1436 1045 188 Cuts at: 9403 10538 11219 11410 826 Size: 1135 681 191 1471 Fragments arranged by size: 1996 1724 1716 1471 1436 1135 1045 681 253 219 191 188 SfiI GGCCn_nnn'nGGCC Cuts at: 4655 7876 4655 Size: 3221 8834 SfoI GGC'GCC Cuts at: 3470 4327 4735 6604 8067 3470 Size: 857 408 1869 1463 7458 Fragments arranged by size: 7458 1869 1463 857 408 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12055 SgrAI Cr'CCGG_yG Cuts at: 1700 4063 6410 8062 1700 Size: 2363 2347 1652 5693 Fragments arranged by size: 5693 2363 2347 1652 SmaI CCC'GGG Cuts at: 1872 2227 4859 4961 5063 6518 7859 9060 9382 Size: 355 2632 102 102 1455 1341 1201 322 Cuts at: 9382 1872 Size: 4545 Fragments arranged by size: 4545 2632 1455 1341 1201 355 322 102 102 SmlI C'TyrA_G Cuts at: 820 1017 1943 3641 4676 5381 6365 6398 10159 Size: 197 926 1698 1035 705 984 33 3761 Cuts at: 10159 11060 11322 820 Size: 901 262 1553 Fragments arranged by size: 3761 1698 1553 1035 984 926 901 705 262 197 33 SnaBI TAC'GTA Cuts at: 493 9846 493 Size: 9353 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 12055 SphI G_CATG'C Cuts at: 1132 3055 5234 5450 9413 1132 Size: 1923 2179 216 3963 3774 Fragments arranged by size: 3963 3774 2179 1923 216 SrfI GCCC'GGGC Cuts at: 6518 6518 Size: 12055 SspI AAT'ATT Cuts at: 5 52 9979 5 Size: 47 9927 2081 Fragments arranged by size: 9927 2081 47 StuI AGG'CCT Cuts at: 1517 2745 2927 5222 6215 1517 Size: 1228 182 2295 993 7357 Fragments arranged by size: 7357 2295 1228 993 182 StyI C'CwwG_G Cuts at: 513 1065 2412 2508 2556 2976 3822 4281 4473 Size: 552 1347 96 48 420 846 459 192 Cuts at: 4473 4629 4823 5407 5451 5475 5493 5820 5898 Size: 156 194 584 44 24 18 327 78 Cuts at: 5898 7134 7290 7845 8596 8871 9012 9494 513 Size: 1236 156 555 751 275 141 482 3074 Fragments arranged by size: 3074 1347 1236 846 751 584 555 552 482 459 420 327 275 194 192 156 156 141 96 78 48 44 24 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2559 4366 5289 5341 5407 5732 6099 Size: 1255 979 1807 923 52 66 325 367 Cuts at: 6099 6224 6789 6855 7516 7793 8227 8496 8581 Size: 125 565 66 661 277 434 269 85 Cuts at: 8581 8656 8663 8870 8930 10082 10099 10252 10437 Size: 75 7 207 60 1152 17 153 185 Cuts at: 10437 10882 325 Size: 445 1498 Fragments arranged by size: 1807 1498 1255 1152 979 923 661 565 445 434 367 325 277 269 207 185 153 125 85 75 66 66 60 52 17 7 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2772 2850 2997 Size: 274 80 33 51 1443 795 78 147 Cuts at: 2997 3270 5742 10301 12013 96 Size: 273 2472 4559 1712 138 Fragments arranged by size: 4559 2472 1712 1443 795 274 273 147 138 80 78 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2060 2092 5846 5878 6087 6119 7334 7366 9237 Size: 32 3754 32 209 32 1215 32 1871 Cuts at: 9237 9269 12015 12047 2060 Size: 32 2746 32 2068 Fragments arranged by size: 3754 2746 2068 1871 1215 209 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3940 1316 Size: 2624 9431 XbaI T'CTAG_A Cuts at: 9391 9391 Size: 12055 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2023 3077 3919 5153 5458 7486 8131 2023 Size: 1054 842 1234 305 2028 645 5947 Fragments arranged by size: 5947 2028 1234 1054 842 645 305 XhoI C'TCGA_G Cuts at: 1943 5381 1943 Size: 3438 8617 XmaI C'CCGG_G Cuts at: 1870 2225 4857 4959 5061 6516 7857 9058 9380 Size: 355 2632 102 102 1455 1341 1201 322 Cuts at: 9380 1870 Size: 4545 Fragments arranged by size: 4545 2632 1455 1341 1201 355 322 102 102 XmnI GAAnn'nnTTC Cuts at: 3011 10184 3011 Size: 7173 4882 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2113 5737 6786 8664 Size: 53 83 186 936 579 3624 1049 1878 Cuts at: 8664 9863 276 Size: 1199 2468 Fragments arranged by size: 3624 2468 1878 1199 1049 936 579 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BstZ17I EcoRV FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SwaI