Restriction map for
pFN21AB1290
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BfrI
BglII BlnI BpuAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspHI BsrDI BssSI Bst1107I
BstAPI BstXI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FseI FspI HindIII KasI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 690 GACGCC G[GA]^CG[CT]C
AhdI 532 GACAGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 199 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 2194 GGGCCC GGGCC^C
Asp700I 1758 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 532 GACAGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 710 TGGCCA TGG^CCA
BclI 1863 TGATCA T^GATCA
BlpI 651 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 651 GCTCAGC GC^T[ACGT]AGC
BsaAI 182 TACGTG [CT]AC^GT[GA]
BsaHI 690 GACGCC G[GA]^CG[CT]C
BsaI 1359 GGTCTC GGTCTC(1/5)
BsmI 1383 GAATGC GAATGC(1/-1)
Bsp1407I 1930 TGTACA T^GTACA
BspLU11I 1974 ACATGT A^CATGT
BspMI 1248 ACCTGC ACCTGC(4/8)
BsrFI 194 ACCGGC [GA]^CCGG[CT]
BsrGI 1930 TGTACA T^GTACA
BssHII 1686 GCGCGC G^CGCGC
BstBI 561 TTCGAA TT^CGAA
BstEII 1980 GGTTACC G^GT[ACGT]ACC
BstPI 1980 GGTTACC G^GT[ACGT]ACC
CelII 651 GCTCAGC GC^T[ACGT]AGC
Cfr10I 194 ACCGGC [GA]^CCGG[CT]
DraII 2194 GGGCCCC [GA]G^G[ACGT]CC[CT]
Eam1105I 532 GACAGGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2199 CCTGCCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2194 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1980 GGTTACC G^GT[ACGT]ACC
FbaI 1863 TGATCA T^GATCA
HgaI 690 GACGC GACGC(5/10)
Hin1I 690 GACGCC G[GA]^CG[CT]C
HincII 676 GTTAAC GT[CT]^[GA]AC
HindII 676 GTTAAC GT[CT]^[GA]AC
HpaI 676 GTTAAC GTT^AAC
MluNI 710 TGGCCA TGG^CCA
MscI 710 TGGCCA TGG^CCA
NheI 240 GCTAGC G^CTAGC
NspI 1974 ACATGT [GA]CATG^[CT]
NspV 561 TTCGAA TT^CGAA
PflMI 1258 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfuI 561 TTCGAA TT^CGAA
SspBI 1930 TGTACA T^GTACA
Van91I 1258 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 793 TCTAGA T^CTAGA
XcmI 1346 CCACCCCAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1758 GAAGCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AocI 835 CCTGAGG CC^T[ACGT]AGG
2203 CCTCAGG
BanI 925 GGCACC G^G[CT][GA]CC
1414 GGCACC
BglI 29 GCCACAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1606 GCCGAGCAGGC
BsmFI 2185 GGGAC GGGAC(10/14)
1342 GTCCC
Bsu36I 835 CCTGAGG CC^T[ACGT]AGG
2203 CCTCAGG
EaeI 27 CGGCCA [CT]^GGCC[GA]
710 TGGCCA
Eco81I 835 CCTGAGG CC^T[ACGT]AGG
2203 CCTCAGG
FauI 1656 GCGGG CCCGC(4/6)
1671 GCGGG
HaeII 1659 GGCGCT [GA]GCGC^[CT]
1784 AGCGCC
HphI 1876 GGTGA GGTGA(8/7)
2126 TCACC
MseI 677 TTAA T^TAA
1400 TTAA
MslI 765 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1922 CACTTCCATG
PvuII 728 CAGCTG CAG^CTG
1633 CAGCTG
SacI 635 GAGCTC GAGCT^C
1892 GAGCTC
SfaNI 512 GCATC GCATC(5/9)
751 GCATC
SmaI 209 CCCGGG CCC^GGG
1616 CCCGGG
SmlI 394 CTTGAG C^T[CT][GA]AG
1807 CTTGAG
TfiI 555 GAATC G^A[AT]TC
2157 GAATC
Tru9I 677 TTAA T^TAA
1400 TTAA
Tsp45I 2002 GTGAC ^GT[GC]AC
2125 GTCAC
XmaCI 209 CCCGGG C^CCGGG
1616 CCCGGG
XmaI 209 CCCGGG C^CCGGG
1616 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrBI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfcI StyI TaqI TseI
Tsp509I TspRI TthHB8I XhoII